{"id":3059,"date":"2023-01-08T13:36:39","date_gmt":"2023-01-08T19:36:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=3059"},"modified":"2023-01-20T16:49:11","modified_gmt":"2023-01-20T22:49:11","slug":"calbryte-590-am","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calbryte-590-am\/","title":{"rendered":"Calbryte\u2122 590 AM"},"content":{"rendered":"\n<p>El ensayo de flujo de calcio intracelular es un m\u00e9todo ampliamente utilizado para monitorear las v\u00edas de transducci\u00f3n de se\u00f1ales y la detecci\u00f3n de alto rendimiento de receptores acoplados a prote\u00edna G (GPCR) y objetivos de canales de calcio. Seguido por Rhod-2 que se introdujo en 1989, Rhod-4 y Cal-590 se desarrollaron m\u00e1s tarde con una relaci\u00f3n se\u00f1al\/fondo mejorada, y se convirtieron en los indicadores de Ca2+ fluorescentes rojos ampliamente utilizados para microscop\u00eda confocal, citometr\u00eda de flujo y aplicaciones de detecci\u00f3n de alto rendimiento En c\u00e9lulas CHO y HEK, Rhod-4 y Cal-590 tienen una respuesta de calcio celular que es 10 veces m\u00e1s sensible que Rhod-2 AM. Sin embargo, Cal-590 y Rhod-4 son a\u00fan menos sensibles al calcio en las c\u00e9lulas que los indicadores de calcio fluorescentes verdes correspondientes (p. ej., Fluo-8 y Cal-520). <\/p>\n\n\n\n<p>Calbryte\u2122 590 es una nueva generaci\u00f3n de indicadores fluorescentes rojos para la medici\u00f3n del calcio intracelular. u relaci\u00f3n se\u00f1al\/fondo muy mejorada y sus propiedades de retenci\u00f3n intracelular hacen de Calbryte\u2122 590 AM el indicador fluorescente rojo m\u00e1s robusto para evaluar GPCR y canales de calcio, as\u00ed como para detectar sus agonistas y antagonistas en c\u00e9lulas vivas. Al igual que otras marcas de colorante AM en c\u00e9lulas, el Calbryte\u2122 590 AM \u00e9ster no es fluorescente y una vez que ingresa a la c\u00e9lula, es hidrolizado por la esterasa intracelular y se activa. El indicador activado es una mol\u00e9cula polar que ya no es capaz de difundirse libremente a trav\u00e9s de la membrana celular, esencialmente atrapada dentro de las c\u00e9lulas.\u00a0 <\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>AAT-20700<\/td><td>Calbryte\u2122 590 AM<\/td><td>2 x 50 ug<\/td><\/tr><tr><td>AAT-20701<\/td><td>Calbryte\u2122 590 AM<\/td><td>10 x 50 ug<\/td><\/tr><tr><td>AAT-20702<\/td><td>Calbryte\u2122 590 AM<\/td><td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:12px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-1 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/safety-data-sheet-sds\/safety-data-sheet-for-calbryte-590-am-catalog-20700.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">SDS<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/protocol-and-product-information-sheet-pis\/protocol-for-calbryte-590-am-version-97c8221cc0.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Protocol<\/a><\/p>\n<\/div>\n\n\n\n<p>Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar a &nbsp;(&lt; -15 \u00b0C), Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n<div style=\"height:46px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Plataforma<\/mark><\/p>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n<\/td><td>540 nm<\/td><\/tr><tr><td>Emisi\u00f3n<\/td><td>590 nm<\/td><\/tr><tr><td>Corte<\/td><td>570 nm<\/td><\/tr><tr><td>Placa Recomendada<\/td><td>Paredes negras \/ fondo claro <\/td><\/tr><tr><td>Especificaciones Instrumento<\/td><td>Modo de lectura inferior \/ Manejo de l\u00edquidos programable<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Microscopio de Flourescencia<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n<\/td><td>TRITC\/Cy3<\/td><\/tr><tr><td>Emisi\u00f3n<\/td><td>TRITC\/Cy3<\/td><\/tr><tr><td>Placa recomendada<\/td><td>Paredes negras \/ fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:41px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Espectro<\/mark><\/p>\n\n\n\n<p><em>Abrir en&nbsp;<a href=\"https:\/\/www.aatbio.com\/fluorescence-excitation-emission-spectrum-graph-viewer\/calbryte_590\" target=\"_blank\" rel=\"noopener\" title=\"\">Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77.png\" alt=\"\" class=\"wp-image-3063\" width=\"857\" height=\"487\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77.png 1010w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77-300x170.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77-768x436.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77-600x341.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-77-280x160.png 280w\" sizes=\"(max-width: 857px) 100vw, 857px\" \/><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Propiedades espectrales<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n (nm)<\/td><td>581<\/td><\/tr><tr><td>Emisi\u00f3n (nm)<\/td><td>593<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:59px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-2 wp-block-group has-background\" style=\"background-color:#fafafa\"><div class=\"wp-block-group__inner-container\">\n<h6><strong>PREPARACION DE SOLUCIONES DE STOCK<\/strong><\/h6>\n\n\n\n<p style=\"font-size:16px\"><em>A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n<p style=\"font-size:16px\"><em>Soluci\u00f3n madre Calbryte\u2122 590 AM<\/em><br>Prepare una soluci\u00f3n madre de 2 a 5 mM de Calbryte\u2122 590 AM en DMSO anhidro de alta calidad.<\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div class=\"wp-container-3 wp-block-group has-background\" style=\"background-color:#fafafa\"><div class=\"wp-block-group__inner-container\">\n<h6><strong>PREPARACION DE SOLUCIONES DE TRABAJO<\/strong><\/h6>\n\n\n\n<p style=\"font-size:16px\"><em>Soluci\u00f3n de trabajo Calbryte\u2122 590 AM<\/em><br>El d\u00eda del experimento, disuelva Calbryte\u2122 590 AM en DMSO o descongele una al\u00edcuota de la soluci\u00f3n madre indicadora a temperatura ambiente. Prepare una soluci\u00f3n de trabajo de tinte de 2 a 20 \u00b5M en un buffer de su elecci\u00f3n (p. ej., tamp\u00f3n Hanks y Hepes) con Pluronic\u00ae F-127 al 0,04 %. Para la mayor\u00eda de las l\u00edneas celulares, se recomienda Calbryte\u2122 590 AM a una concentraci\u00f3n final de 4 a 5 \u03bcM. La concentraci\u00f3n exacta de indicadores necesarios para la carga de c\u00e9lulas debe determinarse emp\u00edricamente.<br><strong>Nota<\/strong>: El detergente no i\u00f3nico Pluronic\u00ae F-127 a veces se usa para aumentar la solubilidad acuosa de Calbryte\u2122 590 AM. Se puede comprar una variedad de soluciones Pluronic\u00ae F-127 de AAT Bioquest.<br><strong>Nota<\/strong>: Si sus c\u00e9lulas contienen transportadores de aniones org\u00e1nicos, se puede agregar probenecid (1-2 mM) a la soluci\u00f3n de trabajo del colorante (la concentraci\u00f3n final en el pocillo ser\u00e1 de 0.5-1 mM) para reducir la fuga de los indicadores desesterificados. Se puede comprar una variedad de productos de probenecid ReadiUse\u2122, que incluyen sal de sodio soluble en agua y soluci\u00f3n estabilizada, en AAT Bioquest.<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:57px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Calculadora<\/mark><\/p>\n\n\n\n<p>Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<br>Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de Calbryte\u2122 590 AM a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n<figure id=\"product_calculator_table\" class=\"wp-block-table\"><table><tbody><tr><td><\/td><td><strong>0.1 mg<\/strong><\/td><td><strong>0.5 mg<\/strong><\/td><td><strong>1 mg<\/strong><\/td><td><strong>5 mg<\/strong><\/td><td><strong>10 mg<\/strong><\/td><\/tr><tr><td><strong>1 mM<\/strong><\/td><td>82.05 \u00b5L<\/td><td>410.25 \u00b5L<\/td><td>820.499 \u00b5L<\/td><td>4.102 mL<\/td><td>8.205 mL<\/td><\/tr><tr><td><strong>5 mM<\/strong><\/td><td>16.41 \u00b5L<\/td><td>82.05 \u00b5L<\/td><td>164.1 \u00b5L<\/td><td>820.499 \u00b5L<\/td><td>1.641 mL<\/td><\/tr><tr><td><strong>10 mM<\/strong><\/td><td>8.205 \u00b5L<\/td><td>41.025 \u00b5L<\/td><td>82.05 \u00b5L<\/td><td>410.25 \u00b5L<\/td><td>820.499 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Imagen<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78.png\" alt=\"\" class=\"wp-image-3064\" width=\"686\" height=\"474\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78.png 999w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78-300x207.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78-768x530.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78-600x414.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-78-232x160.png 232w\" sizes=\"(max-width: 686px) 100vw, 686px\" \/><\/figure>\n\n\n\n<p style=\"font-size:16px\"><strong>Figura 1.<\/strong>  Se midi\u00f3 una dosis-respuesta de ATP en c\u00e9lulas CHO-K1 con Calbryte\u2122 590 AM. Se sembraron c\u00e9lulas CHO-K1 durante la noche a 50.000 c\u00e9lulas\/100 \u00b5l\/pozo en una placa costar de 96 pocillos de pared negra\/fondo transparente. Se a\u00f1adieron 100 \u00b5l de 10 \u00b5g\/ml de Calbryte\u2122 590 AM en buffer HH con probenecid y se incubaron durante 60 min a 37 \u00b0C. A continuaci\u00f3n, la soluci\u00f3n de tinte se elimin\u00f3 y se reemplaz\u00f3 con 200 \u00b5l de buffer HH\/pozo. FlexStation 3 a\u00f1adi\u00f3 ATP (50 \u00b5l\/pocillo) para alcanzar las concentraciones finales indicadas.<\/p>\n\n\n\n<div style=\"height:43px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Productos Relacionados<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:16px\"><table><tbody><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520-potassium-salt\">Calbryte\u2122 520, potassium salt<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-590-potassium-salt\">Calbryte\u2122 590, potassium salt<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-630-potassium-salt\">Calbryte\u2122 630, potassium salt<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520l-potassium-salt\">Calbryte\u2122-520L, potassium salt<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520xl-azide\">Calbryte\u2122-520XL azide<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520xl-potassium-salt\">Calbryte\u2122-520XL, potassium salt<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520xl-dextran\">Calbryte\u2122-520XL-Dextran<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/chemical-phosphorylation-reagent-i-cpr-i\">Chemical Phosphorylation Reagent I (CPR I)<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-mitochondrial-hydroxyl-radical-detection-kit-red-fluorescence\">Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cal-green-1-hexapotassium-salt\">Cal Green\u2122 1, hexapotassium salt<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:51px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211124721017290\" target=\"_blank\" rel=\"noreferrer noopener\">The influence of spontaneous and visual activity on the development of direction selectivity maps in mouse retina<\/a><br><strong>Authors:&nbsp;<\/strong>Tiriac, Alexandre and Bistrong, Karina and Pitcher, Miah N and Tworig, Joshua M and Feller, Marla B<br><strong>Journal:&nbsp;<\/strong>Cell reports&nbsp;(2022):&nbsp;110225<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/rupress.org\/jem\/article\/213035\/Microglia-modulate-blood-flow-neurovascular\" target=\"_blank\" rel=\"noreferrer noopener\">Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions<\/a><br><strong>Authors:&nbsp;<\/strong>Cs{\\&#8217;a}sz{\\&#8217;a}r, Eszter and L{\\&#8217;e}n{\\&#8217;a}rt, Nikolett and Cser{\\&#8217;e}p, Csaba and K{\\&#8221;o}rnyei, Zsuzsanna and Fekete, Rebeka and P{\\&#8217;o}sfai, Bal{\\&#8217;a}zs and Bal{\\&#8217;a}zsfi, Di{\\&#8217;a}na and Hangya, Bal{\\&#8217;a}zs and Schwarcz, Anett D and Szabadits, Eszter and others,<br><strong>Journal:&nbsp;<\/strong>Journal of Experimental Medicine&nbsp;(2022)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.biorxiv.org\/content\/biorxiv\/early\/2021\/03\/07\/2021.03.05.434122.full.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Neurodegeneration in human brain organoids infected with herpes simplex virus type 1<\/a><br><strong>Authors:&nbsp;<\/strong>Rybak-Wolf, Agnieszka and Wyler, Emanuel and Legnini, Ivano and Loewa, Anna and Gla{\\v{z}}ar, Petar and Kim, Seung Joon and Pentimalli, Tancredi Massimo and Martinez, Anna Oliveras and Beyersdorf, Benjamin and Woehler, Andrew and others,<br><strong>Journal:&nbsp;<\/strong>bioRxiv&nbsp;(2021)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/dukespace.lib.duke.edu\/dspace\/bitstream\/handle\/10161\/22967\/Le_duke_0066D_16017.pdf%3Fsequence%3D1\" target=\"_blank\" rel=\"noreferrer noopener\">Investigating the Molecular Mechanisms of TMEM16F&#8211;a Ca2+ Activated Phospholipid Scramblase and Ion Channel<\/a><br><strong>Authors:&nbsp;<\/strong>Le, Trieu Phuong Hai<br><strong>Journal:&nbsp;<\/strong>(2021)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.nature.com\/articles\/s41467-021-24739-9\" target=\"_blank\" rel=\"noreferrer noopener\">KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals<\/a><br><strong>Authors:&nbsp;<\/strong>Thillaiappan, Nagendra Babu and Smith, Holly A and Atakpa-Adaji, Peace and Taylor, Colin W<br><strong>Journal:&nbsp;<\/strong>Nature communications&nbsp;(2021):&nbsp;1&#8211;13<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.jbc.org\/content\/early\/2020\/07\/24\/jbc.AC120.014872.full.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Evidence that polyphenols do not inhibit the phospholipid scramblase TMEM16F<\/a><br><strong>Authors:&nbsp;<\/strong>Le, Trieu and Le, Son C and Zhang, Yang and Liang, Pengfei and Yang, Huanghe<br><strong>Journal:&nbsp;<\/strong>Journal of Biological Chemistry&nbsp;(2020):&nbsp;12537&#8211;12544<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2020.10.08.329169v2.full\" target=\"_blank\" rel=\"noreferrer noopener\">A fluorescent sensor for spatiotemporally resolved endocannabinoid dynamics in vitro and in vivo<\/a><br><strong>Authors:&nbsp;<\/strong>Dong, Ao and He, Kaikai and Dudok, Barna and Farrell, Jordan S and Guan, Wuqiang and Liput, Daniel J and Puhl, Henry L and Cai, Ruyi and Duan, Jiali and Albarran, Eddy and others,<br><strong>Journal:&nbsp;<\/strong>BioRxiv&nbsp;(2020)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211124720314728\" target=\"_blank\" rel=\"noreferrer noopener\">Ca2+ Release by IP3 Receptors Is Required to Orient the Mitotic Spindle<\/a><br><strong>Authors:&nbsp;<\/strong>Lagos-Cabr{\\&#8217;e}, Raul and Ivanova, Adelina and Taylor, Colin W<br><strong>Journal:&nbsp;<\/strong>Cell reports&nbsp;(2020):&nbsp;108483<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.science.org\/doi\/full\/10.1126\/sciadv.aba0310\" target=\"_blank\" rel=\"noreferrer noopener\">TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development<\/a><br><strong>Authors:&nbsp;<\/strong>Zhang, Yang and Le, Trieu and Grabau, Ryan and Mohseni, Zahra and Kim, Hoejeong and Natale, David R and Feng, Liping and Pan, Hua and Yang, Huanghe<br><strong>Journal:&nbsp;<\/strong>Science advances&nbsp;(2020):&nbsp;eaba0310<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6986806\/\" target=\"_blank\" rel=\"noreferrer noopener\">Biased modulators of NMDA receptors control channel opening and ion selectivity<\/a><br><strong>Authors:&nbsp;<\/strong>Perszyk, Riley E and Swanger, Sharon A and Shelley, Chris and Khatri, Alpa and Fernandez-Cuervo, Gabriela and Epplin, Matthew P and Zhang, Jing and Le, Phuong and B{\\&#8221;u}low, Pernille and Garnier-Amblard, Ethel and others,<br><strong>Journal:&nbsp;<\/strong>Nature chemical biology&nbsp;(2020):&nbsp;188&#8211;196<\/p>\n\n\n\n<div style=\"height:61px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Referencias<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\">A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux<br><strong>Authors:&nbsp;<\/strong>Bailey S, Macardle PJ.<br><strong>Journal:&nbsp;<\/strong>J Immunol Methods&nbsp;(2006):&nbsp;220<\/p>\n\n\n\n<p style=\"font-size:14px\">Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy<br><strong>Authors:&nbsp;<\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.<br><strong>Journal:&nbsp;<\/strong>Gen Physiol Biophys&nbsp;(2004):&nbsp;357<\/p>\n\n\n\n<p style=\"font-size:14px\">Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry<br><strong>Authors:&nbsp;<\/strong>Patel H, Porter RH, Palmer AM, Croucher MJ.<br><strong>Journal:&nbsp;<\/strong>Br J Pharmacol&nbsp;(2003):&nbsp;671<\/p>\n\n\n\n<p style=\"font-size:14px\">Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics<br><strong>Authors:&nbsp;<\/strong>Loughrey CM, MacEachern KE, Cooper J, Smith GL.<br><strong>Journal:&nbsp;<\/strong>Cell Calcium&nbsp;(2003):&nbsp;1<\/p>\n\n\n\n<p style=\"font-size:14px\">A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification<br><strong>Authors:&nbsp;<\/strong>Zhang Z, Kitching P.<br><strong>Journal:&nbsp;<\/strong>J Virol Methods&nbsp;(2000):&nbsp;187<\/p>\n\n\n\n<p style=\"font-size:14px\">Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang<br><strong>Authors:&nbsp;<\/strong>Rockwell PL, Storey BT.<br><strong>Journal:&nbsp;<\/strong>Mol Reprod Dev&nbsp;(2000):&nbsp;335<\/p>\n\n\n\n<p style=\"font-size:14px\">MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells<br><strong>Authors:&nbsp;<\/strong>Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.<br><strong>Journal:&nbsp;<\/strong>Am J Physiol Gastrointest Liver Physiol&nbsp;(2000):&nbsp;G522<\/p>\n\n\n\n<p style=\"font-size:14px\">Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue<br><strong>Authors:&nbsp;<\/strong>Walczysko P, Wagner E, Albrechtova JT.<br><strong>Journal:&nbsp;<\/strong>Cell Calcium&nbsp;(2000):&nbsp;23<\/p>\n\n\n\n<p style=\"font-size:14px\">[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 &#8211; a comparison with frog skeletal muscle fibres<br><strong>Authors:&nbsp;<\/strong>Wohlfart B., undefined<br><strong>Journal:&nbsp;<\/strong>Acta Physiol Scand&nbsp;(2000):&nbsp;1<\/p>\n\n\n\n<p style=\"font-size:14px\">Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations<br><strong>Authors:&nbsp;<\/strong>Rockwell PL, Storey BT.<br><strong>Journal:&nbsp;<\/strong>Mol Reprod Dev&nbsp;(1999):&nbsp;418<\/p>\n\n\n\n<div style=\"height:52px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Aplication Notes (en Ingles)<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/a-comparison-of-fluorescent-red-calcium-indicators-for-detecting-intracellular-calcium-mobilization-in-cho-cells\" target=\"_blank\" rel=\"noopener\" title=\"\">A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/calbryte-trade-520-calbryte-trade-590-and-calbryte-trade-630-calcium-detection-reagents\" target=\"_blank\" rel=\"noopener\" title=\"\">Calbryte\u2122 520, Calbryte\u2122 590 and Calbryte\u2122 630 Calcium Detection Reagents<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/introducing-calbryte-series\" target=\"_blank\" rel=\"noopener\" title=\"\">Introducing Calbryte\u2122 Series<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/a-comparison-of-fluorescent-red-calcium-indicators-for-detecting-intracellular-calcium-mobilization-in-cho-cells\" target=\"_blank\" rel=\"noopener\" title=\"\">A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/calbryte-trade-520-calbryte-trade-590-and-calbryte-trade-630-calcium-detection-reagents\" target=\"_blank\" rel=\"noopener\" title=\"\">Calbryte\u2122 520, Calbryte\u2122 590 and Calbryte\u2122 630 Calcium Detection Reagents<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Kit de ensayo de Flujo de calcio intracelular Calbryte\u2122 590 AM. Este ensayo es un m\u00e9todo ampliamente utilizado para monitorear las v\u00edas de transducci\u00f3n de se\u00f1ales y la detecci\u00f3n de alto rendimiento de receptores acoplados a prote\u00edna G (GPCR) y objetivos de canales de calcio. <\/p>\n","protected":false},"featured_media":3064,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3059"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3059\/revisions"}],"predecessor-version":[{"id":3751,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3059\/revisions\/3751"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3064"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3059"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3059"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}