{"id":3047,"date":"2023-01-08T12:37:15","date_gmt":"2023-01-08T18:37:15","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=3047"},"modified":"2023-01-20T16:50:17","modified_gmt":"2023-01-20T22:50:17","slug":"calbryte-520-am","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calbryte-520-am\/","title":{"rendered":"Calbryte\u2122 520 AM"},"content":{"rendered":"\n<p>El ensayo de flujo de calcio intracelular es un m\u00e9todo ampliamente utilizado para monitorear las v\u00edas de transducci\u00f3n de se\u00f1ales y la detecci\u00f3n de alto rendimiento de receptores acoplados a prote\u00edna G (GPCR) y objetivos de canales de calcio. Seguido por Fluo-3 que se introdujo en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron m\u00e1s tarde con una relaci\u00f3n se\u00f1al\/fondo mejorado y se convirtieron en los indicadores de Ca2+ ampliamente utilizados para microscop\u00eda confocal, citometr\u00eda de flujo y aplicaciones de detecci\u00f3n de alto rendimiento. Sin embargo, todav\u00eda hay algunos problemas graves con Fluo-4. Por ejemplo, En cuanto a Fluo-3, en la mayor\u00eda de los ensayos de calcio intracelular con Fluo-4 AM, se requiere probenecid para evitar que el Fluo-4 cargado en las c\u00e9lulas se escape de las c\u00e9lulas. compromete los resultados del ensayo ya que est\u00e1 bien documentado que el probenecid tiene una variedad de efectos celulares complicados. Calbryte\u2122 520, AM es un nuevo indicador fluorescente y permeable a las c\u00e9lulas para la medici\u00f3n del calcio intracelular Al igual que otros colorantes esteres AM, Calbryte\u2122 520 AM no es fluorescente ni activable. <\/p>\n\n\n\n<p>Una vez que Calbryte\u2122 520 AM ingresa a la c\u00e9lula, es f\u00e1cilmente hidrolizado por la esterasa intracelular donde se activa y responde al calcio. El indicador activado ahora es una mol\u00e9cula polar que es incapaz de difundirse libremente a trav\u00e9s de la membrana celular, esencialmente atrap\u00e1ndolo dentro de la c\u00e9lula. Al unirse a los iones de calcio, Calbryte\u2122 520 produce una se\u00f1al de fluorescencia brillante con una relaci\u00f3n se\u00f1al\/fondo extremadamente alta. Tiene la misma longitud de onda de excitaci\u00f3n y emisi\u00f3n que Fluo-4, por lo que la misma configuraci\u00f3n de ensayo de Fluo-4 se puede aplicar f\u00e1cilmente a los ensayos de calcio basados \u200b\u200ben Calbryte\u2122 520. Su relaci\u00f3n se\u00f1al\/fondo muy mejorada y sus propiedades de retenci\u00f3n intracelular hacen de Calbryte\u2122 520 AM el indicador m\u00e1s robusto para evaluar los objetivos de los canales de calcio y GPCR, as\u00ed como para detectar sus agonistas y antagonistas en c\u00e9lulas vivas.&nbsp; &nbsp;<\/p>\n\n\n\n<div style=\"height:27px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>AAT-20650<\/td><td>Calbryte\u2122 520 AM<\/td><td>2 x 50 ug<\/td><\/tr><tr><td>AAT-20651<\/td><td>Calbryte\u2122 520 AM<\/td><td>10 x 50 ug<\/td><\/tr><tr><td>AAT-20653<\/td><td>Calbryte\u2122 520 AM<\/td><td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:12px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-1 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/safety-data-sheet-sds\/safety-data-sheet-for-calbryte-520-am-catalog-20650.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">SDS<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/protocol-and-product-information-sheet-pis\/protocol-for-calbryte-520-am-version-74a186ec32.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Protocol<\/a><\/p>\n<\/div>\n\n\n\n<p>Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar a &nbsp;(&lt; -15 \u00b0C), Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n<div style=\"height:46px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Plataforma<\/mark><\/p>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n<\/td><td>490 nm<\/td><\/tr><tr><td>Emisi\u00f3n<\/td><td>252 nm<\/td><\/tr><tr><td>Corte<\/td><td>515 nm<\/td><\/tr><tr><td>Placa Recomendada<\/td><td>Paredes negras \/ fondo claro <\/td><\/tr><tr><td>Especificaciones Instrumento<\/td><td>Modo de lectura inferior \/ Manejo de l\u00edquidos programable<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Cit\u00f3metro de flujo<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Laser de Excitaci\u00f3n<\/td><td>488 nm <\/td><\/tr><tr><td>Filtro de Emisi\u00f3n<\/td><td>530\/30 nm <\/td><\/tr><tr><td>Especificaciones instrumento<\/td><td>Canal FITC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Microscopio de Flourescencia<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n<\/td><td>FITC<\/td><\/tr><tr><td>Emisi\u00f3n<\/td><td>FITC<\/td><\/tr><tr><td>Placa recomendada<\/td><td>Paredes negras \/ fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:41px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Espectro<\/mark><\/p>\n\n\n\n<p><em>Abrir en&nbsp;<a href=\"https:\/\/www.aatbio.com\/fluorescence-excitation-emission-spectrum-graph-viewer\/calbryte_520\" target=\"_blank\" rel=\"noopener\" title=\"\">Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74.png\" alt=\"\" class=\"wp-image-3048\" width=\"860\" height=\"488\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74.png 1010w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74-300x170.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74-768x436.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74-600x341.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-74-280x160.png 280w\" sizes=\"(max-width: 860px) 100vw, 860px\" \/><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Propiedades espectrales<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n (nm)<\/td><td>493<\/td><\/tr><tr><td>Emisi\u00f3n (nm)<\/td><td>515<\/td><\/tr><tr><td>Rendimiento cu\u00e1ntico<\/td><td>0.75<sup>1<\/sup><\/td><\/tr><\/tbody><\/table><figcaption><sup>1<\/sup> Calcium bound (pH 7.2)<\/figcaption><\/figure>\n\n\n\n<div style=\"height:59px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-2 wp-block-group has-background\" style=\"background-color:#fafafa\"><div class=\"wp-block-group__inner-container\">\n<h6><strong>PREPARACION DE SOLUCIONES DE STOCK<\/strong><\/h6>\n\n\n\n<p style=\"font-size:16px\"><em>A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n<p style=\"font-size:16px\"><em>Soluci\u00f3n madre Calbryte\u2122 520 AM<\/em><br>Prepare una soluci\u00f3n madre de 2 a 5 mM de Calbryte\u2122 520 AM en DMSO anhidro.<\/p>\n<\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div class=\"wp-container-3 wp-block-group has-background\" style=\"background-color:#fafafa\"><div class=\"wp-block-group__inner-container\">\n<h6><strong>PREPARACION DE SOLUCIONES DE TRABAJO<\/strong><\/h6>\n\n\n\n<p style=\"font-size:16px\"><em>Soluci\u00f3n de trabajo Calbryte\u2122 520 AM<\/em><br>El d\u00eda del experimento, disuelva Calbryte\u2122 520 AM en DMSO o descongele una al\u00edcuota de la soluci\u00f3n madre indicadora a temperatura ambiente. Prepare una soluci\u00f3n de trabajo de tinte de 2 a 20 \u00b5M en un buffer de su elecci\u00f3n (p. ej., Hanks y Hepes) con Pluronic\u00ae F-127 al 0,04 %. Para la mayor\u00eda de las l\u00edneas celulares, se recomienda Calbryte\u2122 520 AM a una concentraci\u00f3n final de 4 a 5 \u03bcM. La concentraci\u00f3n exacta de indicadores necesarios para la carga de c\u00e9lulas debe determinarse emp\u00edricamente.<\/p>\n\n\n\n<p style=\"font-size:16px\"><br><strong>Nota<\/strong>: El detergente no i\u00f3nico Pluronic\u00ae F-127 a veces se usa para aumentar la solubilidad acuosa de Calbryte\u2122 520 AM. Se puede comprar una variedad de soluciones Pluronic\u00ae F-127 de AAT Bioquest.<br><strong>Nota<\/strong>: Si sus c\u00e9lulas contienen transportadores de aniones org\u00e1nicos, se puede agregar probenecid (1-2 mM) a la soluci\u00f3n de trabajo del colorante (la concentraci\u00f3n final en el pocillo ser\u00e1 de 0,5-1 mM) para reducir la fuga de los indicadores desesterificados. Se puede comprar una variedad de productos de probenecid ReadiUse\u2122, que incluyen sal de sodio soluble en agua y soluci\u00f3n estabilizada, en AAT Bioquest.<\/p>\n<\/div><\/div>\n\n\n\n<div style=\"height:57px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Calculadora<\/mark><\/p>\n\n\n\n<p>Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<br>Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de Calbryte\u2122 520 AM a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n<figure id=\"product_calculator_table\" class=\"wp-block-table\"><table><tbody><tr><td><\/td><td><strong>0.1 mg<\/strong><\/td><td><strong>0.5 mg<\/strong><\/td><td><strong>1 mg<\/strong><\/td><td><strong>5 mg<\/strong><\/td><td><strong>10 mg<\/strong><\/td><\/tr><tr><td><strong>1 mM<\/strong><\/td><td>91.667 \u00b5L<\/td><td>458.337 \u00b5L<\/td><td>916.674 \u00b5L<\/td><td>4.583 mL<\/td><td>9.167 mL<\/td><\/tr><tr><td><strong>5 mM<\/strong><\/td><td>18.333 \u00b5L<\/td><td>91.667 \u00b5L<\/td><td>183.335 \u00b5L<\/td><td>916.674 \u00b5L<\/td><td>1.833 mL<\/td><\/tr><tr><td><strong>10 mM<\/strong><\/td><td>9.167 \u00b5L<\/td><td>45.834 \u00b5L<\/td><td>91.667 \u00b5L<\/td><td>458.337 \u00b5L<\/td><td>916.674 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Imagenes<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75.png\" alt=\"\" class=\"wp-image-3049\" width=\"633\" height=\"351\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75.png 900w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75-300x167.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75-768x427.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75-600x333.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-75-280x156.png 280w\" sizes=\"(max-width: 633px) 100vw, 633px\" \/><figcaption>Fig. 1<\/figcaption><\/figure>\n\n\n\n<p style=\"font-size:16px\"><strong>Figura 1.<\/strong>  La respuesta de ATP se midi\u00f3 en c\u00e9lulas CHO-K1 usando Calbryte\u2122 520 AM (Cat No. 20653) y Fluo-4, AM (Cat No. 20550). Se sembraron c\u00e9lulas CHO-K1 50.000 c\u00e9lulas\/100 \u00b5l\/pozo durante la noche a en una placa costar de 96 pocillos de pared negra\/fondo transparente. Se a\u00f1adieron a los pozos 100 \u00b5l de 10 \u00b5g\/ml de Calbryte\u2122 520 AM en buffer HH con probenecid o 10 \u00b5g\/ml de Fluo-4, AM en buffer HH con probenecid y se incubaron durante 45 minutos a 37 \u00b0C. Ambas soluciones de carga de tinte se eliminaron y se reemplazaron con 200 \u00b5l de buffer HH\/pocillo. Se a\u00f1adi\u00f3 ATP (50 \u00b5l\/pocillo) para lograr la concentraci\u00f3n final indicada de 10 \u00b5M. Las im\u00e1genes fueron adquiridas en un microscopio Keyence en el canal FITC.<\/p>\n\n\n\n<div style=\"height:58px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76.png\" alt=\"\" class=\"wp-image-3051\" width=\"528\" height=\"486\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76.png 1000w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76-300x276.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76-768x707.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76-600x553.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/01\/image-76-174x160.png 174w\" sizes=\"(max-width: 528px) 100vw, 528px\" \/><figcaption>Fig. 2<\/figcaption><\/figure>\n\n\n\n<p style=\"font-size:16px\"><strong>Figura 2.<\/strong> La respuesta a la dosis de carbacol se midi\u00f3 en c\u00e9lulas CHO-M1 con Calbryte\u2122 520 AM y Fluo-4 AM. Se sembraron c\u00e9lulas CHO-M1 durante la noche a 50.000 c\u00e9lulas\/100 \u00b5l\/pocillo en una placa costar de 96 pocillos de pared negra\/fondo transparente. Se a\u00f1adieron 100 \u00b5l de 10 \u00b5g\/ml de Calbryte\u2122 520 AM en buffer HH o 10 \u00b5g\/ml de Fluo-4 en buffer HH y se incubaron durante 45 minutos a 37 \u00b0C. A continuaci\u00f3n, la soluci\u00f3n de carga de tinte se elimin\u00f3 y se reemplaz\u00f3 con 200 \u00b5l de buffer HH\/pocillo. FlexStation 3 a\u00f1adi\u00f3 carbacol (50 \u00b5l\/pocillo) para alcanzar las concentraciones finales indicadas.<\/p>\n\n\n\n<div style=\"height:55px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Formatos Alternativos<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:16px\"><table><tbody><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-green-fluorescence\" target=\"_blank\" rel=\"noopener\" title=\"\">Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-orange-fluorescence\">C<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-orange-fluorescence\" target=\"_blank\" rel=\"noopener\" title=\"\">ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescenc<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-orange-fluorescence\">e*<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-red-fluorescence\">C<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-red-fluorescence\" target=\"_blank\" rel=\"noopener\" title=\"\">ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescenc<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-red-fluorescence\">e*<\/a><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-optimized-for-flow-cytometry\">C<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-optimized-for-flow-cytometry\" target=\"_blank\" rel=\"noopener\" title=\"\">ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometr<\/a><a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-optimized-for-flow-cytometry\">y*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:48px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Productos Relacionados<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:16px\"><table><tbody><tr><td>Nombre<\/td><td>Excitation (nm)<\/td><td>Emission (nm)<\/td><td>Quantum yield<\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-590-am\" target=\"_blank\" rel=\"noreferrer noopener\">Calbryte\u2122 590 AM<\/a><\/td><td>581<\/td><td>593<\/td><td>&#8211;<\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-630-am\" target=\"_blank\" rel=\"noreferrer noopener\">Calbryte\u2122 630 AM<\/a><\/td><td>607<\/td><td>624<\/td><td>&#8211;<\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520l-am\" target=\"_blank\" rel=\"noreferrer noopener\">Calbryte\u2122-520L AM<\/a><\/td><td>493<\/td><td>515<\/td><td>0.75<sup>1<\/sup><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/calbryte-520xl-am\" target=\"_blank\" rel=\"noreferrer noopener\">Calbryte\u2122-520XL AM<\/a><\/td><td>493<\/td><td>515<\/td><td>0.75<sup>1<\/sup><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/cal-520-am\" target=\"_blank\" rel=\"noreferrer noopener\">Cal-520\u00ae, AM<\/a><\/td><td>493<\/td><td>515<\/td><td>0.75<sup>1<\/sup><\/td><\/tr><tr><td><a href=\"https:\/\/www.aatbio.com\/products\/mag-520-am\" target=\"_blank\" rel=\"noreferrer noopener\">Mag-520\u2122 AM<\/a><\/td><td>506<\/td><td>525<\/td><td>&#8211;<\/td><\/tr><\/tbody><\/table><figcaption><sup>1<\/sup> Calcium bound (pH 7.2)<\/figcaption><\/figure>\n\n\n\n<div style=\"height:58px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\"><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><em>Ver todas las 166 bibliografias:&nbsp;<\/em><a href=\"https:\/\/www.aatbio.com\/resources\/citation-explorer?catalog=20650\">Citation Explorer<\/a><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211124721017290\" target=\"_blank\" rel=\"noreferrer noopener\">The influence of spontaneous and visual activity on the development of direction selectivity maps in mouse retina<\/a><br><strong>Authors:&nbsp;<\/strong>Tiriac, Alexandre and Bistrong, Karina and Pitcher, Miah N and Tworig, Joshua M and Feller, Marla B<br><strong>Journal:&nbsp;<\/strong>Cell reports&nbsp;(2022):&nbsp;110225<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/arxiv.org\/pdf\/2202.13462\" target=\"_blank\" rel=\"noreferrer noopener\">Temporal signals drive the emergence of multicellular information networks<\/a><br><strong>Authors:&nbsp;<\/strong>Li, Guanyu and LeFebre, Ryan and Starman, Alia and Chappell, Patrick and Mugler, Andrew and Sun, Bo<br><strong>Journal:&nbsp;<\/strong>arXiv preprint arXiv:2202.13462&nbsp;(2022)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/link.springer.com\/article\/10.1007\/s12015-021-10319-3\" target=\"_blank\" rel=\"noreferrer noopener\">Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte Differentiation<\/a><br><strong>Authors:&nbsp;<\/strong>Cheng, Yu-Che and Huang, Chi-Jung and Ku, Wei-Chi and Guo, Shu-Lin and Tien, Lu-Tai and Lee, Yih-Jing and Chien, Chih-Cheng<br><strong>Journal:&nbsp;<\/strong>Stem Cell Reviews and Reports&nbsp;(2022):&nbsp;1&#8211;14<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.nature.com\/articles\/s41467-022-28930-4\" target=\"_blank\" rel=\"noreferrer noopener\">iRhom pseudoproteases regulate ER stress-induced cell death through IP3 receptors and BCL-2<\/a><br><strong>Authors:&nbsp;<\/strong>Dulloo, Iqbal and Atakpa-Adaji, Peace and Yeh, Yi-Chun and Levet, Cl{\\&#8217;e}mence and Muliyil, Sonia and Lu, Fangfang and Taylor, Colin W and Freeman, Matthew<br><strong>Journal:&nbsp;<\/strong>Nature Communications&nbsp;(2022):&nbsp;1&#8211;18<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.researchgate.net\/profile\/Sandra-Postic\/publication\/361582778_pH-Dependence_of_Glucose-_Dependent_Activity_of_Beta_Cell_Networks_in_Acute_Mouse_Pancreatic_Tissue_Slice\/links\/62bab7a1f9dee438e8c7f51c\/pH-Dependence-of-Glucose-Dependent-Activity-of-Beta-Cell-Networks-in-Acute-Mouse-Pancreatic-Tissue-Slice.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">pH-Dependence of Glucose-Dependent Activity of Beta Cell Networks in Acute Mouse Pancreatic Tissue Slice<\/a><br><strong>Authors:&nbsp;<\/strong>Postic, S and Gosak, M and Tsai, WH and Pfabe, J and Sarikas, S and Stoz\u02c7er, A and Koros\u02c7ak, D and Yang, SB and Slak Rupnik, M<br><strong>Journal:&nbsp;<\/strong>Front. Endocrinol. 13: 916688. doi: 10.3389\/fendo&nbsp;(2022)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.258796\/2122724\/jcs258796.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">CPVT-associated calmodulin variants N53I and A102V dysregulate Ca2+ signalling via different mechanisms<\/a><br><strong>Authors:&nbsp;<\/strong>Prakash, Ohm and Held, Marie and McCormick, Liam F and Gupta, Nitika and Lian, Lu-Yun and Antonyuk, Svetlana and Haynes, Lee P and Thomas, N Lowri and Helassa, Nordine<br><strong>Journal:&nbsp;<\/strong>Journal of Cell Science&nbsp;(2022):&nbsp;jcs258796<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/adma.202102641\" target=\"_blank\" rel=\"noreferrer noopener\">Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology<\/a><br><strong>Authors:&nbsp;<\/strong>Uslu, Fazil E and Davidson, Christopher D and Mailand, Erik and Bouklas, Nikolaos and Baker, Brendon M and Sakar, Mahmut Selman<br><strong>Journal:&nbsp;<\/strong>Advanced Materials&nbsp;(2021):&nbsp;2102641<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.hindawi.com\/journals\/omcl\/2021\/5552530\/\" target=\"_blank\" rel=\"noreferrer noopener\">Pharmic Activation of PKG2 Alleviates Diabetes-Induced Osteoblast Dysfunction by Suppressing PLC$\\beta$1-Ca2+-Mediated Endoplasmic Reticulum Stress<\/a><br><strong>Authors:&nbsp;<\/strong>Jia, Tingting and Wang, Ya-nan and Feng, Yao and Wang, Chenchen and Zhang, Dongjiao and Xu, Xin<br><strong>Journal:&nbsp;<\/strong>Oxidative Medicine and Cellular Longevity&nbsp;(2021)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/conservancy.umn.edu\/bitstream\/handle\/11299\/225110\/Li_umn_0130E_22835.pdf%3Fsequence%3D1\" target=\"_blank\" rel=\"noreferrer noopener\">Mechanistic Investigation of Cell Cryopreservation Aided by Raman Spectroscopy: Challenges and Solutions for hiPSC Technologies<\/a><br><strong>Authors:&nbsp;<\/strong>Li, Rui<br><strong>Journal:&nbsp;<\/strong>(2021)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/journals.plos.org\/ploscompbiol\/article%3Fid%3D10.1371\/journal.pcbi.1009002\" target=\"_blank\" rel=\"noreferrer noopener\">NMDA receptor inhibition increases, synchronizes, and stabilizes the collective pancreatic beta cell activity: Insights through multilayer network analysis<\/a><br><strong>Authors:&nbsp;<\/strong>{\\v{S}}terk, Marko and Kri{\\v{z}}an{\\v{c}}i{\\&#8217;c} Bombek, Lidija and Skelin Klemen, Ma{\\v{s}}a and Slak Rupnik, Marjan and Marhl, Marko and Sto{\\v{z}}er, Andra{\\v{z}} and Gosak, Marko<br><strong>Journal:&nbsp;<\/strong>PLoS Computational Biology&nbsp;(2021):&nbsp;e1009002<\/p>\n\n\n\n<div style=\"height:58px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Referencias<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><em>Ver todas las 63 referencias:&nbsp;<\/em><a href=\"https:\/\/www.aatbio.com\/resources\/citation-explorer?catalog=20650\">Citation Explorer<\/a><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1357272517301863\" target=\"_blank\" rel=\"noreferrer noopener\">Calreticulin regulates TGF-\u03b21-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling<\/a><br><strong>Authors:&nbsp;<\/strong>Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling<br><strong>Journal:&nbsp;<\/strong>The International Journal of Biochemistry &amp; Cell Biology&nbsp;(2017)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.nrronline.org\/article.asp%3Fissn%3D1673-5374%3Byear%3D2017%3Bvolume%3D12%3Bissue%3D6%3Bspage%3D945%3Bepage%3D952%3Baulast%3DLu\" target=\"_blank\" rel=\"noreferrer noopener\">Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells<\/a><br><strong>Authors:&nbsp;<\/strong>Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined<br><strong>Journal:&nbsp;<\/strong>Neural Regeneration Research&nbsp;(2017):&nbsp;945<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.nature.com\/articles\/srep41781\" target=\"_blank\" rel=\"noreferrer noopener\">Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels<\/a><br><strong>Authors:&nbsp;<\/strong>Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang<br><strong>Journal:&nbsp;<\/strong>Scientific Reports&nbsp;(2017):&nbsp;41781<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.ijcem.com\/files\/ijcem0051456.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Dexmedetomidine reduces hypoxia\/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons<\/a><br><strong>Authors:&nbsp;<\/strong>Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei<br><strong>Journal:&nbsp;<\/strong>Int J Clin Exp Med&nbsp;(2017):&nbsp;6861&#8211;6868<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12409\/full\" target=\"_blank\" rel=\"noreferrer noopener\">Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection<\/a><br><strong>Authors:&nbsp;<\/strong>Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun<br><strong>Journal:&nbsp;<\/strong>Journal of cellular and molecular medicine&nbsp;(2015):&nbsp;581&#8211;594<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0006291X15007561\" target=\"_blank\" rel=\"noreferrer noopener\">The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia\/reperfusion injury<\/a><br><strong>Authors:&nbsp;<\/strong>Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia<br><strong>Journal:&nbsp;<\/strong>Biochemical and biophysical research communications&nbsp;(2015):&nbsp;537&#8211;542<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/link.springer.com\/article\/10.1007\/s00702-014-1166-6\" target=\"_blank\" rel=\"noreferrer noopener\">Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats<\/a><br><strong>Authors:&nbsp;<\/strong>Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min<br><strong>Journal:&nbsp;<\/strong>Journal of Neural Transmission&nbsp;(2014):&nbsp;593&#8211;600<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.nrronline.org\/article.asp?issn=1673-5374;year=2014;volume=9;issue=22;spage=2002;epage=2007;aulast=Li\" target=\"_blank\" rel=\"noreferrer noopener\">Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem\/progenitor cells<\/a><br><strong>Authors:&nbsp;<\/strong>Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined<br><strong>Journal:&nbsp;<\/strong>Neural regeneration research&nbsp;(2014):&nbsp;2002<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/europepmc.org\/articles\/pmc4458643\" target=\"_blank\" rel=\"noreferrer noopener\">Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.<\/a><br><strong>Authors:&nbsp;<\/strong>Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui<br><strong>Journal:&nbsp;<\/strong>International journal of ophthalmology&nbsp;(2014):&nbsp;441&#8211;447<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjd.12108\/full\" target=\"_blank\" rel=\"noreferrer noopener\">Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve&#8211;mast cell interaction in a hapten-induced mouse model of atopic dermatitis<\/a><br><strong>Authors:&nbsp;<\/strong>Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A<br><strong>Journal:&nbsp;<\/strong>British Journal of Dermatology&nbsp;(2013):&nbsp;771&#8211;778<\/p>\n\n\n\n<div style=\"height:52px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Aplication Notes (en Ingles)<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/calbryte-trade-520-calbryte-trade-590-and-calbryte-trade-630-calcium-detection-reagents\" target=\"_blank\" rel=\"noopener\" title=\"\">Calbryte\u2122 520, Calbryte\u2122 590 and Calbryte\u2122 630 Calcium Detection Reagents<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/introducing-calbryte-series\" target=\"_blank\" rel=\"noopener\" title=\"\">Introducing Calbryte\u2122 Series<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/the-eight-best-green-fluorescent-calcium-indicators\" target=\"_blank\" rel=\"noopener\" title=\"\">The Eight Best Green Fluorescent Calcium Indicators<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/what-s-a-ratiometric-indicator\" target=\"_blank\" rel=\"noopener\" title=\"\">What&#8217;s A Ratiometric Indicator<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/calbryte-trade-520-calbryte-trade-590-and-calbryte-trade-630-calcium-detection-reagents\" target=\"_blank\" rel=\"noopener\" title=\"\">Calbryte\u2122 520, Calbryte\u2122 590 and Calbryte\u2122 630 Calcium Detection Reagents<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Kit de ensayo de Flujo de calcio intracelular Mod. Calbryte\u2122 520 AM. Este es un m\u00e9todo ampliamente utilizado para monitorear las v\u00edas de transducci\u00f3n de se\u00f1ales y la detecci\u00f3n de alto rendimiento de receptores acoplados a prote\u00edna G (GPCR) y objetivos de canales de calcio. <\/p>\n","protected":false},"featured_media":3050,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3047"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":10,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3047\/revisions"}],"predecessor-version":[{"id":3752,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3047\/revisions\/3752"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3050"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3047"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3047"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}