{"id":3033,"date":"2023-01-08T11:23:27","date_gmt":"2023-01-08T17:23:27","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3033"},"modified":"2023-02-28T22:06:06","modified_gmt":"2023-03-01T04:06:06","slug":"screen-quest-calbryte-520-probenecid-free-and-wash-free-calcium-assay-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/screen-quest-calbryte-520-probenecid-free-and-wash-free-calcium-assay-kit\/","title":{"rendered":"Screen Quest\u2122 Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit"},"content":{"rendered":"\n

Los ensayos de flujo de calcio son los m\u00e9todos preferidos en el descubrimiento de f\u00e1rmacos para la detecci\u00f3n de receptores acoplados a prote\u00edna G (GPCR). El kit de ensayo de calcio Screen Quest\u2122 Calbryte-520 sin probenecid y sin lavado proporciona un ensayo homog\u00e9neo m\u00e1s robustobasado en fluorescencia para detectar la movilizaci\u00f3n de calcio intracelular. <\/p>\n\n\n\n

Las c\u00e9lulas que expresan  GPCR de inter\u00e9s que se\u00f1aliza a trav\u00e9s del calcio est\u00e1n precargadas con nuestro Calbryte\u2122-520 AM patentado que puede atravesar la membrana celular. Calbryte\u2122-520 AM es el indicador de calcio m\u00e1s brillante disponible para la detecci\u00f3n HTS. Una vez dentro de la c\u00e9lula, los grupos bloqueadores lipof\u00edlicos de Calbryte\u2122-520 AM son escindidos por esterasa celular no espec\u00edfica, lo que da como resultado un tinte fluorescente cargado negativamente que permanece dentro de las c\u00e9lulas y su fluorescencia aumenta considerablemente al unirse al calcio. Cuando las c\u00e9lulas se estimulan con compuestos de detecci\u00f3n, el receptor se\u00f1ala la liberaci\u00f3n de calcio intracelular, lo que aumenta en gran medida la fluorescencia de Calbryte\u2122-520 AM. Las caracter\u00edsticas de su excelente retenci\u00f3n celular, alta sensibilidad y aumentos de fluorescencia de 100 a 250 veces (cuando forma complejos con calcio) hacen de Calbryte\u2122-520 AM un indicador ideal para la medici\u00f3n de calcio celular. Calbryte\u2122-520 AM es el \u00fanico tinte de calcio que no requiere probenecid para una mejor retenci\u00f3n celular. <\/p>\n\n\n\n

Este kit de ensayo de calcio Screen Quest\u2122 Calbryte-520 sin probenecid y sin lavado proporciona el m\u00e9todo de ensayo m\u00e1s optimizado para monitorear los receptores acoplados a prote\u00edna G (GPCR) y los canales de calcio con l\u00edneas celulares fr\u00e1giles o dif\u00edciles. El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pozos o de 384 pozos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n.\u00a0 <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-36317<\/td>Screen Quest\u2122 Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>1 placa<\/td><\/tr>
AAT-36318<\/td>Screen Quest\u2122 Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>10 placas<\/td><\/tr>
AAT-36319<\/td>Screen Quest\u2122 Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>100 placas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Cutoff<\/td>515 nm<\/td><\/tr>
Placa recomendada<\/td>Paredes negras, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior \/ Manejo de l\u00edquidos programable<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Otros Instrumentos: ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, NOVOStar, ViewLux<\/p>\n\n\n\n

<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Calbryte\u2122 520 AM<\/td>1 vial<\/td><\/tr>
Componente B: 10X Pluronic\u00ae F127 Plus<\/td>1 vial (1 ml)<\/td><\/tr>
Componente C: HHBS (Hanks\u2019 buffer con 20 mM Hepes)<\/td>1 vial (9 ml)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>493<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>515<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.751<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Calcium bound (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n madre Calbryte\u2122 520 AM<\/em>
    Agregue 20 \u00b5L (Cat. # 36317) o 200 \u00b5L (Cat. # 36318 y # 36319) de DMSO en el vial de Calbryte\u2122 520 AM (Componente A) y mezcle bien.
    Tenga en cuenta que 20 \u00b5L de soluci\u00f3n madre Calbryte\u2122 520 AM es suficiente para una placa. La soluci\u00f3n madre de Calbryte\u2122 520 AM sin usar se puede dividir en al\u00edcuotas y almacenar a < -20 \u00b0C durante m\u00e1s de un mes si los tubos est\u00e1n sellados herm\u00e9ticamente.
    Nota<\/strong> Proteger de la luz y evitar ciclos repetidos de congelaci\u00f3n-descongelaci\u00f3n.<\/li>
  2. Buffer de ensayo (1X)<\/em>
    Mezcle 9 mL de HHBS (Componente C, no incluido en el kit Cat. # 36319) con 1 mL de 10X Pluronic\u00ae F127 Plus (10X) (Componente B) y mezcle bien.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    \n\n\n\n

    PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

    \n

    Soluci\u00f3n de carga de tinte Calbryte\u2122 520 AM<\/em>
    Agregue 20 \u00b5L de soluci\u00f3n madre Calbryte\u2122 520 AM en 10 mL de buffer de ensayo (1X) y mezcle bien.
    Nota<\/strong>: Esta soluci\u00f3n de trabajo es estable durante al menos 2 horas a temperatura ambiente.
    Tenga en cuenta que 10 ml de soluci\u00f3n de carga de colorante son suficientes para una placa de 96 pocillos.<\/p>\n\n\n\n

    Para obtener guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/figcaption><\/figure>\n\n\n\n

    Figura 1<\/strong>. Comparaci\u00f3n de la respuesta de la se\u00f1al fluorescente del receptor P2Y end\u00f3geno al ATP en c\u00e9lulas CHO-K1. Se sembraron 50.000 c\u00e9lulas CHO-K1\/100 \u00b5l\/poczo  durante la noche a en una placa costar de 96 pozos s de pared negra\/fondo transparente. La respuesta del flujo de calcio se midi\u00f3 con Screen Quest\u2122 Calbryte\u2122 520 Probenecid-Free and Wash-Free Calcium Assay Kit, FLIPR Calcium 4 Assay Kit y Fluo-4 Direct Calcium Assay kit. FlexStation 3 a\u00f1adi\u00f3 ATP (50 \u00b5l\/pozo) para alcanzar las concentraciones finales indicadas.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Relacionados<\/mark><\/p>\n\n\n\n

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    Screen Quest\u2122 Fluo-8 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
    Screen Quest\u2122 Fura-2 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
    Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit *Medium Removal*<\/a><\/td><\/tr>
    Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

    Ver todas las 15 bibliografias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Selective activation of TRPA1 ion channels by nitrobenzene skin sensitizers DNFB and DNCB<\/a>
    Authors: <\/strong>Wu, Han and Niu, Canyang and Qu, Yaxuan and Sun, Xiaoying and Wang, KeWei
    Journal: <\/strong>Journal of Biological Chemistry (2022)<\/p>\n\n\n\n

    Preferential Gs protein coupling of the galanin Gal1 receptor in the $\\mu$-opioid-Gal1 receptor heterotetramer<\/a>
    Authors: <\/strong>De Oliveira, Paulo A and Moreno, Estefan{\\’\\i}a and Casajuana-Martin, Nil and Casad{\\’o}-Anguera, Ver{\\`o}nica and Cai, Ning-Sheng and Camacho-Hernandez, Gisela Andrea and Zhu, Hu and Bonifazi, Alessandro and Hall, Matthew D and Weinshenker, David and others,
    Journal: <\/strong>Pharmacological Research (2022): 106322<\/p>\n\n\n\n

    Selective activation of TRPA1 ion channel by skin sensitizer nitrobenzene DNFB<\/a>
    Authors: <\/strong>Wu, Han and Qu, Yaxuan and Sun, Xiaoying and Wang, Kewei
    Journal: <\/strong>Authorea Preprints (2021)<\/p>\n\n\n\n

    Diquafosol tetrasodium elicits total cholesterol release from rabbit meibomian gland cells via P2Y 2 purinergic receptor signalling<\/a>
    Authors: <\/strong>Endo, Ken-ichi and Sakamoto, Asuka and Fujisawa, Koushi
    Journal: <\/strong>Scientific reports (2021): 1–8<\/p>\n\n\n\n

    Identification of two natural coumarin enantiomers for selective inhibition of TRPV2 channels<\/a>
    Authors: <\/strong>Zhou, Qiqi and Shi, Yuntao and Qi, Hang and Liu, Huijie and Wei, Ningning and Jiang, Yong and Wang, KeWei
    Journal: <\/strong>The FASEB Journal (2020): 12338–12353<\/p>\n\n\n\n

    Calreticulin regulates TGF-\u03b21-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling<\/a>
    Authors: <\/strong>Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
    Journal: <\/strong>The International Journal of Biochemistry & Cell Biology (2017)<\/p>\n\n\n\n

    Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells<\/a>
    Authors: <\/strong>Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
    Journal: <\/strong>Neural Regeneration Research (2017): 945<\/p>\n\n\n\n

    Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels<\/a>
    Authors: <\/strong>Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
    Journal: <\/strong>Scientific Reports (2017): 41781<\/p>\n\n\n\n

    Dexmedetomidine reduces hypoxia\/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons<\/a>
    Authors: <\/strong>Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
    Journal: <\/strong>Int J Clin Exp Med (2017): 6861–6868<\/p>\n\n\n\n

    Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection<\/a>
    Authors: <\/strong>Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
    Journal: <\/strong>Journal of cellular and molecular medicine (2015): 581–594<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las 172 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Calcium imaging of cortical neurons using Fura-2 AM
    Authors: <\/strong>Barreto-Chang OL, Dolmetsch RE.
    Journal: <\/strong>J Vis Exp. (2009)<\/p>\n\n\n\n

    A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
    Authors: <\/strong>Bailey S, Macardle PJ.
    Journal: <\/strong>J Immunol Methods (2006): 220<\/p>\n\n\n\n

    Measurement of [Ca2+]i in whole cell suspensions using fura-2
    Authors: <\/strong>Hirst RA, Harrison C, Hirota K, Lambert DG.
    Journal: <\/strong>Methods Mol Biol (2006): 37<\/p>\n\n\n\n

    Load of calcium probe Fura -2\/AM in Escherichia coli cells
    Authors: <\/strong>Shao M, Wang HM, Liu ZH, Shen P, Cai RX.
    Journal: <\/strong>Wei Sheng Wu Xue Bao (2005): 805<\/p>\n\n\n\n

    Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
    Authors: <\/strong>Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
    Journal: <\/strong>FEMS Microbiol Lett (2004): 49<\/p>\n\n\n\n

    Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy
    Authors: <\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
    Journal: <\/strong>Gen Physiol Biophys (2004): 357<\/p>\n\n\n\n

    Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2
    Authors: <\/strong>McConnell G, Riis E.
    Journal: <\/strong>Phys Med Biol (2004): 4757<\/p>\n\n\n\n

    Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2
    Authors: <\/strong>Xu T, Yang W, Huo XL, Song T.
    Journal: <\/strong>J Biochem Biophys Methods (2004): 219<\/p>\n\n\n\n

    Cytoplasmic calcium measurement in rotavirus enterotoxin-enhanced green fluorescent protein (NSP4-EGFP) expressing cells loaded with Fura-2
    Authors: <\/strong>Berkova Z, Morris AP, Estes MK.
    Journal: <\/strong>Cell Calcium (2003): 55<\/p>\n\n\n\n

    Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
    Authors: <\/strong>Patel H, Porter RH, Palmer AM, Croucher MJ.
    Journal: <\/strong>Br J Pharmacol (2003): 671<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a>
    Introducing Calbryte\u2122 Series<\/a>
    A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
    A Meta-Analysis of Common Calcium Indicators<\/a>
    A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de calcio sin probenecid y sin lavado Modelo Screen Quest\u2122 Calbryte-520. Este kit proporciona el m\u00e9todo de ensayo m\u00e1s optimizado para monitorear los receptores acoplados a prote\u00edna G (GPCR) y los canales de calcio con l\u00edneas celulares fr\u00e1giles o dif\u00edciles.<\/p>\n","protected":false},"featured_media":3034,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3033"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":10,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3033\/revisions"}],"predecessor-version":[{"id":7581,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3033\/revisions\/7581"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3034"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3033"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3033"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}