{"id":2951,"date":"2023-01-07T12:34:18","date_gmt":"2023-01-07T18:34:18","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2951"},"modified":"2023-01-19T13:20:33","modified_gmt":"2023-01-19T19:20:33","slug":"cell-meter-fluorimetric-mitochondrial-superoxide-activity-assay-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-fluorimetric-mitochondrial-superoxide-activity-assay-kit\/","title":{"rendered":"Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit"},"content":{"rendered":"\n

Las mitocondrias son los principales productores de super\u00f3xido celular. La producci\u00f3n de super\u00f3xido baja a moderada es fundamental para la regulaci\u00f3n adecuada de muchos procesos celulares esenciales, incluida la expresi\u00f3n g\u00e9nica, la transducci\u00f3n de se\u00f1ales y la adaptaci\u00f3n muscular al entrenamiento de ejercicios de resistencia. La producci\u00f3n descontrolada de super\u00f3xido mitocondrial puede desencadenar un da\u00f1o oxidativo celular que contribuye a la patogenia de una amplia variedad de trastornos, como el c\u00e1ncer, las enfermedades cardiovasculares, las enfermedades neurodegenerativas y el envejecimiento. La detecci\u00f3n de super\u00f3xido mitocondrial intracelular es de importancia central para comprender la regulaci\u00f3n celular redox adecuada y el impacto de su desregulaci\u00f3n en diversas patolog\u00edas. <\/p>\n\n\n\n

El kit de ensayo de actividad de super\u00f3xido mitocondrial fluorim\u00e9trico Cell Meter\u2122 utiliza nuestro exclusivo indicador de super\u00f3xido para cuantificar el nivel de super\u00f3xido en c\u00e9lulas vivas. MitoROS\u2122 580 es permeable a las c\u00e9lulas vivas y puede dirigirse r\u00e1pida y selectivamente al super\u00f3xido en las mitocondrias. Genera fluorescencia roja cuando reacciona con super\u00f3xido. El kit de detecci\u00f3n de super\u00f3xido intracelular fluorim\u00e9trico Cell Meter\u2122 proporciona un ensayo fluorim\u00e9trico sensible de un solo paso para detectar super\u00f3xido mitocondrial en c\u00e9lulas vivas con una hora de incubaci\u00f3n. Este kit se puede utilizar en lectores de microplacas de fluorescencia y aplicaciones de microscop\u00eda de fluorescencia. <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22971<\/td>Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit<\/td>200 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>540 nm<\/td><\/tr>
Emisi\u00f3n<\/td>590 nm<\/td><\/tr>
Cutoff<\/td>570 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: MitoROS\u2122 580<\/td>1 vial <\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 botella (20 mL)<\/td><\/tr>
Componente C: DMSO<\/td>1 vial (100 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n madre de MitoROS\u2122 580 (500X): <\/em> Agregue 50 \u00b5L de DMSO (Componente C) en el vial de MitoROS\u2122 580 (Componente A) y mezcle bien para preparar una soluci\u00f3n madre de MitoROS\u2122 580 500X. Proteger de la luz. Nota: 25 \u00b5L de soluci\u00f3n madre 500X MitoROS\u2122 580 es suficiente para 1 placa. Para el almacenamiento, selle los tubos herm\u00e9ticamente.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    \n\n\n\n
    \n

    PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

    Agregue 25 \u03bcL de soluci\u00f3n madre de MitoROS\u2122 580 500X en 10 mL de buffer de ensayo (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo de MitoROS\u2122 580. Nota: Esta soluci\u00f3n de trabajo MitoROS\u2122 580 es estable durante al menos 2 horas a temperatura ambiente.<\/p>\n\n\n\n

    Para obtener guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/em><\/figcaption><\/figure>\n\n\n\n

    Figura 1.<\/strong> Im\u00e1genes de fluorescencia de la medici\u00f3n de super\u00f3xido en c\u00e9lulas HeLa utilizando el kit de detecci\u00f3n de super\u00f3xido intracelular fluorim\u00e9trico Cell Meter\u2122 (n.\u00b0 de cat\u00e1logo 22971). Se colocaron 100 \u00b5l de Cell Meter en  100.000 c\u00e9lulas HeLa\/pozo\/ incubando durante la noche en una placa de fondo transparente\/pared negra de 96 pozos<\/a>[mp1]<\/a> . Tratamiento con AMA: las c\u00e9lulas se trataron con antimicina A (AMA) 50 \u00b5M a 37 \u00b0C durante 30 minutos y luego se incubaron con MitoROS\u2122 580 durante 1 hora. Control no tratado: las c\u00e9lulas HeLa se incubaron con MitoROS\u2122 580 a 37 \u00b0C durante 1 hora sin tratamiento con AMA. La se\u00f1al de fluorescencia se midi\u00f3 utilizando un microscopio de fluorescencia con un filtro TRITC.<\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"
    Fig. 2<\/em><\/figcaption><\/figure>\n\n\n\n

    Figura 2<\/strong>. Detecci\u00f3n de super\u00f3xido intracelular en c\u00e9lulas HeLa utilizando el kit de detecci\u00f3n de super\u00f3xido intracelular fluorim\u00e9trico Cell Meter\u2122 (Cat#22971). Se sembraron c\u00e9lulas HeLa a 100.000 c\u00e9lulas\/pocillo\/100 \u00b5l durante la noche en una placa de fondo transparente\/pared negra de 96 pocillos. Las c\u00e9lulas se incubaron con piocianina (Pyo) 50 \u00b5M; Antimicina A (AMA) 50 \u00b5M o sin tratamiento (Control) a 37 \u00baC durante 30 minutos. A continuaci\u00f3n, las c\u00e9lulas se incubaron con MitoROS\u2122 580 a 37 \u00baC durante 1 hora. La se\u00f1al de fluorescencia se control\u00f3 a Ex\/Em = 540\/590 nm (corte = 570 nm) con modo de lectura inferior utilizando un lector de microplacas CLARIOstar (BMG Labtech).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Alternativos <\/mark><\/p>\n\n\n\n

    Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
    Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Flow Cytometry*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

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    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

    GRP75-faciliated Mitochondria-associated ER Membrane (MAM) Integrity controls Cisplatin-resistance in Ovarian Cancer Patients<\/a>
    Authors: <\/strong>Li, Jing and Qi, Fangzheng and Su, Huishan and Zhang, Chuanshan and Zhang, Qing and Chen, Ying and Chen, Ping and Su, Linjia and Chen, Yanan and Yang, Yuqi and others,
    Journal: <\/strong>International journal of biological sciences (2022): 2914<\/p>\n\n\n\n

    High-glucose-induced apoptosis, ROS production and pro-inflammatory response in cardiomyocytes is attenuated by metformin treatment via PP2A activation<\/a>
    Authors: <\/strong>Cheng, Gang and Li, Lihuan
    Journal: <\/strong>Journal of Biosciences (2020): 1–11<\/p>\n\n\n\n

    Effect of antioxidants on the H2O2-induced premature senescence of human fibroblasts<\/a>
    Authors: <\/strong>Pie{\\’n}kowska, Natalia and Bartosz, Grzegorz and Pichla, Monika and Grzesik-Pietrasiewicz, Michalina and Gruchala, Martyna and Sadowska-Bartosz, Izabela
    Journal: <\/strong>Aging (Albany NY) (2020): 1910<\/p>\n\n\n\n

    Nitroxide Radical-Containing Redox Nanoparticles Protect Neuroblastoma SH-SY5Y Cells against 6-Hydroxydopamine Toxicity<\/a>
    Authors: <\/strong>Pichla, Monika and Pulaski, {\\L}ukasz and Kania, Katarzyna Dominika and Stefaniuk, Ireneusz and Cieniek, Bogumi{\\l} and Pie{\\’n}kowska, Natalia and Bartosz, Grzegorz and Sadowska-Bartosz, Izabela
    Journal: <\/strong>Oxidative Medicine and Cellular Longevity (2020)<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las 60 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation
    Authors: <\/strong>Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.
    Journal: <\/strong>J Endod (2014): 51<\/p>\n\n\n\n

    Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides
    Authors: <\/strong>Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.
    Journal: <\/strong>J Toxicol Sci (2014): 71<\/p>\n\n\n\n

    Green synthesis of carbon dots with down- and up-conversion fluorescent properties for sensitive detection of hypochlorite with a dual-readout assay
    Authors: <\/strong>Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S.
    Journal: <\/strong>Analyst (2013): 6551<\/p>\n\n\n\n

    Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay
    Authors: <\/strong>Thorn RM, Robinson GM, Reynolds DM.
    Journal: <\/strong>Antimicrob Agents Chemother (2013): 2216<\/p>\n\n\n\n

    Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite
    Authors: <\/strong>Setlow B, Yu J, Li YQ, Setlow P.
    Journal: <\/strong>Lett Appl Microbiol (2013): 259<\/p>\n\n\n\n

    A simple yet effective chromogenic reagent for the rapid estimation of bromate and hypochlorite in drinking water
    Authors: <\/strong>Zhang J, Yang X.
    Journal: <\/strong>Analyst (2013): 434<\/p>\n\n\n\n

    Effect of hypochlorite-based disinfectants on inactivation of murine norovirus and attempt to eliminate or prevent infection in mice by addition to drinking water
    Authors: <\/strong>Takimoto K, Taharaguchi M, Sakai K, Takagi H, Tohya Y, Yamada YK.
    Journal: <\/strong>Exp Anim (2013): 237<\/p>\n\n\n\n

    Use of pyrogallol red and pyranine as probes to evaluate antioxidant capacities towards hypochlorite
    Authors: <\/strong>Perez-Cruz F, Cortes C, Atala E, Bohle P, Valenzuela F, Olea-Azar C, Speisky H, Aspee A, Lissi E, Lopez-Alarcon C, Bridi R.
    Journal: <\/strong>Molecules (2013): 1638<\/p>\n\n\n\n

    Enhancement of anti-cholinesterase activity of aqueous samples by hypochlorite oxidation for monitoring traces of organophosphorus pesticides in water
    Authors: <\/strong>Kanno A, Kawakami T, Takahashi Y, Onodera S.
    Journal: <\/strong>J Toxicol Sci (2012): 389<\/p>\n\n\n\n

    Colorimetric determination of hypochlorite with unmodified gold nanoparticles through the oxidation of a stabilizer thiol compound
    Authors: <\/strong>Zhang J, Wang X, Yang X.
    Journal: <\/strong>Analyst (2012): 2806<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

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    A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de actividad de super\u00f3xido mitocondrial fluorim\u00e9trico Cell Meter\u2122 esta optimizado para uso en lectores de microplacas y utiliza nuestro exclusivo indicador de super\u00f3xido para cuantificar el nivel de super\u00f3xido en c\u00e9lulas vivas.<\/p>\n","protected":false},"featured_media":2956,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2951"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":13,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2951\/revisions"}],"predecessor-version":[{"id":3609,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2951\/revisions\/3609"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2956"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2951"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2951"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}