{"id":2943,"date":"2023-01-07T12:12:33","date_gmt":"2023-01-07T18:12:33","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2943"},"modified":"2023-01-20T16:54:27","modified_gmt":"2023-01-20T22:54:27","slug":"cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-red-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kit-red-fluorescence\/","title":{"rendered":"Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit *Red Fluorescence*"},"content":{"rendered":"\n

Las especies reactivas de ox\u00edgeno (ROS) son subproductos naturales del metabolismo normal del ox\u00edgeno y juegan un papel importante en la se\u00f1alizaci\u00f3n celular. Sin embargo, los niveles de ROS pueden aumentar dram\u00e1ticamente durante los estados relacionados con el estr\u00e9s oxidativo, lo que resulta en un da\u00f1o significativo a las estructuras celulares. <\/p>\n\n\n\n

El papel del estr\u00e9s oxidativo en las enfermedades cardiovasculares, la diabetes, la osteoporosis, los accidentes cerebrovasculares, las enfermedades inflamatorias, las enfermedades neurodegenerativas y el c\u00e1ncer ha sido bien establecido. A trav\u00e9s de las mediciones de ROS, los investigadores pueden determinar c\u00f3mo el estr\u00e9s oxidativo modula diversas v\u00edas intracelulares. El kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122 utiliza nuestro exclusivo sensor de ROS, Amplite\u00ae ROS Red, para cuantificar ROS en c\u00e9lulas vivas. Amplite\u00ae ROS Red es permeable a las c\u00e9lulas y genera fluorescencia roja cuando reacciona con ROS. El ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122 es f\u00e1cil de realizar utilizando un formato optimizado de mezcla y lectura. Proporciona un ensayo fluorim\u00e9trico sensible de un solo paso para detectar ROS intracelulares en c\u00e9lulas vivas con 1-2 horas de incubaci\u00f3n. <\/p>\n\n\n\n

El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pocillos o de 384 pocillos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n sin un paso de separaci\u00f3n. Su se\u00f1al se puede leer f\u00e1cilmente utilizando un lector de microplacas de fluorescencia o un microscopio de fluorescencia. Se puede utilizar para cuantificar las actividades de ROS o detectar inhibidores de ROS. <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22901<\/td>Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit *Red Fluorescence*<\/td>200 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>520 nm<\/td><\/tr>
Emisi\u00f3n<\/td>605 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Juego de filtros Texas Red<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Lector de Microplacas de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>520 nm<\/td><\/tr>
Emisi\u00f3n<\/td>605 nm<\/td><\/tr>
Cutoff<\/td>590 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Amplite\u2122 ROS Red<\/td>1 vial <\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 botella (20 mL)<\/td><\/tr>
Componente C: DMSO<\/td>1 vial (200 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n

PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n madre Amplite\u2122 ROS Red (500X):<\/em>
    Agregue 40 \u00b5L de DMSO (Componente C) en el vial de Amplite\u2122 ROS Red (Componente A) y mezcle bien para preparar una soluci\u00f3n madre de Amplite\u2122 ROS Red 500X. Proteger de la luz. Nota: 20 \u00b5L de soluci\u00f3n madre 500X Amplite\u2122 ROS Red es suficiente para 1 placa. Nota: la porci\u00f3n no utilizada puede dividirse en al\u00edcuotas y almacenarse a < -20 \u00b0C durante m\u00e1s de un mes si los tubos se sellan herm\u00e9ticamente y se mantienen alejados de la luz. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    \n\n\n\n
    \n

    PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

    Agregue 20 \u00b5L de soluci\u00f3n madre 500X Amplite\u2122 ROS Red en 10 mL de buffer de ensayo (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo Amplite\u2122 ROS Red. Nota: Esta soluci\u00f3n de trabajo Amplite\u2122 ROS Red es estable durante al menos 2 horas a temperatura ambiente.<\/p>\n\n\n\n

    Para obtener pautas sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

    <\/div>\n\n\n\n

    Imagen<\/mark><\/p>\n\n\n\n

    \"\"<\/figure>\n\n\n\n

    Figura 1.<\/strong> Detecci\u00f3n de ROS en c\u00e9lulas Jurkat con el kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122. Se sembraron a 300.000 c\u00e9lulas  Jurkat el mismo d\u00eda \/(100 \u00b5l\/pozo) en una placa Costar de 96 pozos de pared negra\/fondo transparente. Se a\u00f1adi\u00f3 la soluci\u00f3n de carga del ensayo de ROS (100 \u00b5l\/pozo) y se incub\u00f3 a 37 \u00b0C con CO2 al 5 % durante 1 hora. Las c\u00e9lulas se trataron con 1 mM de H2O2 o sin H2O2 (control) durante 2 horas. La se\u00f1al de fluorescencia se control\u00f3 a Ex\/Em = 520\/605 nm (corte = 590 nm) con el modo de lectura inferior utilizando un lector de microplacas FlexStation (Molecular Devices).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Alternativos <\/mark><\/p>\n\n\n\n

    Cel<\/a>l Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence<\/a>*<\/a><\/td><\/tr>
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    Ce<\/a>ll Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluorescence<\/a>*<\/a><\/td><\/tr>
    Cell <\/a>Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry<\/a>*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

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    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Ver todas las 25 bibliografias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Automatic flow injection based methodologies for determination of scavenging capacity against biologically relevant reactive species of oxygen and nitrogen
    Authors: <\/strong>Magalhaes LM, Lucio M, Segundo MA, Reis S, Lima JL.
    Journal: <\/strong>Talanta (2009): 1219<\/p>\n\n\n\n

    Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species
    Authors: <\/strong>Amaral S, Oliveira PJ, Ramalho-Santos J.
    Journal: <\/strong>Curr Diabetes Rev (2008): 46<\/p>\n\n\n\n

    Virion disruption by ozone-mediated reactive oxygen species
    Authors: <\/strong>Murray BK, Ohmine S, Tomer DP, Jensen KJ, Johnson FB, Kirsi JJ, Robison RA, O’Neill KL.
    Journal: <\/strong>J Virol Methods (2008): 74<\/p>\n\n\n\n

    The role of mitochondria in reactive oxygen species metabolism and signaling
    Authors: <\/strong>Starkov AA., undefined
    Journal: <\/strong>Ann N Y Acad Sci (2008): 37<\/p>\n\n\n\n

    Sensitive determination of reactive oxygen species by chemiluminescence methods and their application to biological samples and health foods
    Authors: <\/strong>Wada M., undefined
    Journal: <\/strong>Yakugaku Zasshi (2008): 1031<\/p>\n\n\n\n

    Reactive oxygen species and yeast apoptosis
    Authors: <\/strong>Perrone GG, Tan SX, Dawes IW.
    Journal: <\/strong>Biochim Biophys Acta (2008): 1354<\/p>\n\n\n\n

    Measurement of reactive oxygen species in cells and mitochondria
    Authors: <\/strong>Armstrong JS, Whiteman M.
    Journal: <\/strong>Methods Cell Biol (2007): 355<\/p>\n\n\n\n

    Role of reactive oxygen species in mediating hepatic ischemia-reperfusion injury and its therapeutic applications in liver transplantation
    Authors: <\/strong>Zhang W, Wang M, Xie HY, Zhou L, Meng XQ, Shi J, Zheng S.
    Journal: <\/strong>Transplant Proc (2007): 1332<\/p>\n\n\n\n

    Superoxide and derived reactive oxygen species in the regulation of hypoxia-inducible factors
    Authors: <\/strong>Gorlach A, Kietzmann T.
    Journal: <\/strong>Methods Enzymol (2007): 421<\/p>\n\n\n\n

    Reactive oxygen species and superoxide dismutases: role in joint diseases
    Authors: <\/strong>Afonso V, Champy R, Mitrovic D, Collin P, Lomri A.
    Journal: <\/strong>Joint Bone Spine (2007): 324<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las 48 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Automatic flow injection based methodologies for determination of scavenging capacity against biologically relevant reactive species of oxygen and nitrogen
    Authors: <\/strong>Magalhaes LM, Lucio M, Segundo MA, Reis S, Lima JL.
    Journal: <\/strong>Talanta (2009): 1219<\/p>\n\n\n\n

    Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species
    Authors: <\/strong>Amaral S, Oliveira PJ, Ramalho-Santos J.
    Journal: <\/strong>Curr Diabetes Rev (2008): 46<\/p>\n\n\n\n

    Virion disruption by ozone-mediated reactive oxygen species
    Authors: <\/strong>Murray BK, Ohmine S, Tomer DP, Jensen KJ, Johnson FB, Kirsi JJ, Robison RA, O’Neill KL.
    Journal: <\/strong>J Virol Methods (2008): 74<\/p>\n\n\n\n

    The role of mitochondria in reactive oxygen species metabolism and signaling
    Authors: <\/strong>Starkov AA., undefined
    Journal: <\/strong>Ann N Y Acad Sci (2008): 37<\/p>\n\n\n\n

    Sensitive determination of reactive oxygen species by chemiluminescence methods and their application to biological samples and health foods
    Authors: <\/strong>Wada M., undefined
    Journal: <\/strong>Yakugaku Zasshi (2008): 1031<\/p>\n\n\n\n

    Reactive oxygen species and yeast apoptosis
    Authors: <\/strong>Perrone GG, Tan SX, Dawes IW.
    Journal: <\/strong>Biochim Biophys Acta (2008): 1354<\/p>\n\n\n\n

    Measurement of reactive oxygen species in cells and mitochondria
    Authors: <\/strong>Armstrong JS, Whiteman M.
    Journal: <\/strong>Methods Cell Biol (2007): 355<\/p>\n\n\n\n

    Role of reactive oxygen species in mediating hepatic ischemia-reperfusion injury and its therapeutic applications in liver transplantation
    Authors: <\/strong>Zhang W, Wang M, Xie HY, Zhou L, Meng XQ, Shi J, Zheng S.
    Journal: <\/strong>Transplant Proc (2007): 1332<\/p>\n\n\n\n

    Superoxide and derived reactive oxygen species in the regulation of hypoxia-inducible factors
    Authors: <\/strong>Gorlach A, Kietzmann T.
    Journal: <\/strong>Methods Enzymol (2007): 421<\/p>\n\n\n\n

    Reactive oxygen species and superoxide dismutases: role in joint diseases
    Authors: <\/strong>Afonso V, Champy R, Mitrovic D, Collin P, Lomri A.
    Journal: <\/strong>Joint Bone Spine (2007): 324<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
    A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
    A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
    A Meta-Analysis of Common Calcium Indicators<\/a>
    A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de actividad de ROS total intracelular fluorom\u00e9trico Cell Meter\u2122 *Fluorescencia verde*, El kit utiliza nuestro exclusivo sensor de ROS para cuantificar ROS en c\u00e9lulas vivas. <\/p>\n","protected":false},"featured_media":2944,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2943"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2943\/revisions"}],"predecessor-version":[{"id":3756,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2943\/revisions\/3756"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2944"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2943"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}