{"id":2935,"date":"2023-01-07T11:37:27","date_gmt":"2023-01-07T17:37:27","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2935"},"modified":"2023-01-20T16:54:52","modified_gmt":"2023-01-20T22:54:52","slug":"cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kitgreen-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kitgreen-fluorescence\/","title":{"rendered":"Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit *Green Fluorescence*"},"content":{"rendered":"\n

Las especies reactivas de ox\u00edgeno (ROS) son subproductos naturales del metabolismo normal del ox\u00edgeno y juegan un papel importante en la se\u00f1alizaci\u00f3n celular. Sin embargo, durante los estados relacionados con el estr\u00e9s oxidativo, los niveles de ROS pueden aumentar dr\u00e1sticamente. La acumulaci\u00f3n de ROS da como resultado un da\u00f1o significativo a las estructuras celulares. El papel del estr\u00e9s oxidativo en las enfermedades cardiovasculares, la diabetes, la osteoporosis, los accidentes cerebrovasculares, las enfermedades inflamatorias, una serie de enfermedades neurodegenerativas y el c\u00e1ncer ha sido bien establecido. La medici\u00f3n de ROS ayudar\u00e1 a determinar c\u00f3mo el estr\u00e9s oxidativo modula diversas v\u00edas intracelulares. <\/p>\n\n\n\n

El kit de ensayo de ROS fluorim\u00e9trico Cell Meter\u2122 utiliza nuestro exclusivo sensor de ROS para cuantificar ROS en c\u00e9lulas vivas. ROS Green es permeable a las c\u00e9lulas. Genera la fluorescencia verde cuando reacciona con ROS. El kit es un formato de ensayo optimizado de “mezclar y leer” que es compatible con los instrumentos de manejo de l\u00edquidos HTS. El kit de ROS fluorim\u00e9trico Cell Meter\u2122 proporciona un ensayo fluorim\u00e9trico sensible de un solo paso para detectar ROS intracelular en c\u00e9lulas vivas con una hora de incubaci\u00f3n. <\/p>\n\n\n\n

El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pozos o de 384 pozos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n sin un paso de separaci\u00f3n. Su se\u00f1al se puede leer f\u00e1cilmente utilizando un lector de microplacas de fluorescencia o un microscopio de fluorescencia.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22900<\/td>Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*<\/td>200 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Filtro FITC<\/td><\/tr>
Emisi\u00f3n<\/td>Filtro FITC<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Lector de Microplacas de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Cutoff<\/td>515 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Amplite\u2122 ROS Green<\/td>1 vial <\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 botella (20 mL)<\/td><\/tr>
Componente C: DMSO<\/td>1 vial (200 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n madre Amplite\u2122 ROS Green (500X):<\/em>
    Agregue 40 \u00b5L de DMSO (Componente C) en el vial de Amplite\u2122 ROS Green (Componente A) y mezcle bien para preparar una soluci\u00f3n madre de Amplite\u2122 ROS Green 500X. Proteger de la luz. Nota: 20 \u00b5L de soluci\u00f3n madre 500X Amplite\u2122 ROS Green es suficiente para 1 placa. Para el almacenamiento, selle los tubos herm\u00e9ticamente.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    \n\n\n\n
    \n

    PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

    Agregue 20 \u00b5L de soluci\u00f3n madre 500X Amplite\u2122 ROS Green en 10 mL de buffer de ensayo (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo Amplite\u2122 ROS Green. Nota: Esta soluci\u00f3n de trabajo Amplite\u2122 ROS Green es estable durante al menos 2 horas a temperatura ambiente.<\/p>\n\n\n\n

    Para obtener pautas sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/figcaption><\/figure>\n\n\n\n

    Figura 1. . <\/strong>Imagenes de fluorescencia para la detecccion simultanea de oxido nitrico (NO) y ROS total en macr\u00f3fagos RAW 264.7. Las c\u00e9lulas fueron co-te\u00f1idas con Nitrixyte\u2122 Orange (Red) y Amplite\u00ae ROS Green (Green). Las c\u00e9lulas fueron tratadas 20\u00b5g\/ml de lipopolisac\u00e1rido (LPS), 1mM L-arginina (L-Arg) y 50\u00b5M piocianina (Pyo )  y c\u00e9lulas control  sin tratamiento, ambas incubadas a 37\u00b0C por 16 horas . La se\u00f1al de la fluorescencia fue cuantificada usando un microscopio de fluoresencia equipado con TRIC (Nitrixyte\u2122 Orange, Red)  y FITC (Amplite\u00ae ROS Green, Green) simult\u00e1neamente.<\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"
    Fig. 2<\/figcaption><\/figure>\n\n\n\n

    Figura 2<\/strong>. Detecci\u00f3n de ROS en c\u00e9lulas Jurkat con el kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122. Se sembraron c\u00e9lulas Jurkat el mismo d\u00eda a 300.000 c\u00e9lulas\/100 \u00b5l\/pocillo en una placa de 96 pocillos Costar de pared negra\/fondo transparente. Se a\u00f1adi\u00f3 la soluci\u00f3n de carga del ensayo de ROS (100 \u00b5l\/pocillo) y se incub\u00f3 en una incubadora CO2 a 37 \u00b0C al 5 % durante 1 hora. Y luego las c\u00e9lulas se trataron con H2O2 1 mM, 0,1 mM o sin H2O2 durante 30 minutos. La se\u00f1al de fluorescencia se control\u00f3 a Ex\/Em = 490\/525 nm (corte a 515 nm) con el modo de lectura inferior utilizando FlexStation (Molecular Devices).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Alternativos <\/mark><\/p>\n\n\n\n

    Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*<\/a><\/td><\/tr>
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    Ce<\/a>ll Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluoresce<\/a>nce*<\/a><\/td><\/tr>
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    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Ver todas las 29 bibliograf\u00edas: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

    Obstructive sleep apnea-increased DEC1 regulates systemic inflammation and oxidative stress that promotes development of pulmonary arterial hypertension<\/a>
    Authors: <\/strong>Li, Xiaoming and Zhang, Xiang and Hou, Xiaozhi and Bing, Xin and Zhu, Fangyuan and Wu, Xinhao and Guo, Na and Zhao, Hui and Xu, Fenglei and Xia, Ming
    Journal: <\/strong>Apoptosis (2022): 1–15<\/p>\n\n\n\n

    ONC206 has anti-tumorigenic effects in human ovarian cancer cells and in a transgenic mouse model of high-grade serous ovarian cancer<\/a>
    Authors: <\/strong>Tucker, Katherine and Yin, Yajie and Staley, Stuart-Allison and Zhao, Ziyi and Fang, Ziwei and Fan, Yali and Zhang, Xin and Suo, Hongyan and Sun, Wenchuan and Prabhu, Varun Vijay and others,
    Journal: <\/strong>American Journal of Cancer Research (2022): 521<\/p>\n\n\n\n

    Reversal of multidrug resistance by Fissistigma latifolium–derived chalconoid 2-hydroxy-4, 5, 6-trimethoxydihydrochalcone in cancer cell lines overexpressing human P-glycoprotein<\/a>
    Authors: <\/strong>Teng, Yu-Ning and Hung, Chin-Chuan and Kao, Pei-Heng and Chang, Ying-Tzu and Lan, Yu-Hsuan
    Journal: <\/strong>Biomedicine \\& Pharmacotherapy (2022): 113832<\/p>\n\n\n\n

    GRP75-faciliated Mitochondria-associated ER Membrane (MAM) Integrity controls Cisplatin-resistance in Ovarian Cancer Patients<\/a>
    Authors: <\/strong>Li, Jing and Qi, Fangzheng and Su, Huishan and Zhang, Chuanshan and Zhang, Qing and Chen, Ying and Chen, Ping and Su, Linjia and Chen, Yanan and Yang, Yuqi and others,
    Journal: <\/strong>International journal of biological sciences (2022): 2914<\/p>\n\n\n\n

    The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance<\/a>
    Authors: <\/strong>Cai, Dong-Mei and Mei, Fan-Biao and Zhang, Chao-Jun and An, San-Chun and Lv, Rui-Bo and Ren, Guan-Hua and Xiao, Chan-Chan and Long, Long and Huang, Tian-Ren and Deng, Wei
    Journal: <\/strong>Journal of Hepatocellular Carcinoma (2022): 1229–1246<\/p>\n\n\n\n

    Antineoplastic Effects and Mechanisms of a New RGD Chimeric Peptide from Bullfrog Skin on the Proliferation and Apoptosis of B16F10 Cells<\/a>
    Authors: <\/strong>Jiang, Xuan and Zhang, Xin and Fu, Chao and Zhao, Ruili and Jin, Tianming and Liu, Mengyue and Pan, Chenhao and Li, Liu An and Ma, Jifei and Yu, Enyuan and others,
    Journal: <\/strong>The Protein Journal (2021): 1–12<\/p>\n\n\n\n

    ALS-causing SOD1 mutants regulate occludin phosphorylation\/ubiquitination and endocytic trafficking via the ITCH\/Eps15\/Rab5 axis<\/a>
    Authors: <\/strong>Tang, Jingshu and Kang, Yuying and Zhou, Yujun and Li, Xinnan and Lan, Jiaqi and Wu, Lei and Feng, Xinhong and Peng, Ying
    Journal: <\/strong>Neurobiology of Disease (2021): 105315<\/p>\n\n\n\n

    Hydrogen peroxide induces progranulin expression to control neurite outgrowth in HT22 cells<\/a>
    Authors: <\/strong>Shimoda, Ayumu and Tanabe, Takemi and Sato, Tsubasa and Nedachi, Taku
    Journal: <\/strong>Bioscience, Biotechnology, and Biochemistry (2021): 2103–2112<\/p>\n\n\n\n

    Caffeic Acid Attenuates Multi-Drug Resistance in Cancer Cells by Inhibiting Efflux Function of Human P-glycoprotein<\/a>
    Authors: <\/strong>Teng, Yu-Ning and Wang, Charles CN and Liao, Wei-Chieh and Lan, Yu-Hsuan and Hung, Chin-Chuan
    Journal: <\/strong>Molecules (2020): 247<\/p>\n\n\n\n

    Epigallocatechin gallate diminishes cigarette smoke-induced oxidative stress, lipid peroxidation, and inflammation in human bronchial epithelial cells<\/a>
    Authors: <\/strong>Lakshmi, Sowmya P and Reddy, Aravind T and Kodidhela, Lakshmi Devi and Varadacharyulu, N Ch
    Journal: <\/strong>Life Sciences (2020): 118260<\/p>\n\n\n\n

    <\/div>\n\n\n\n

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    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

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    A Meta-Analysis of Common Calcium Indicators<\/a>
    A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de actividad de ROS total intracelular fluorom\u00e9trico Cell Meter\u2122 *Fluorescencia verde*, El kit utiliza nuestro exclusivo sensor de ROS para cuantificar ROS en c\u00e9lulas vivas. <\/p>\n","protected":false},"featured_media":2941,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2935"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2935\/revisions"}],"predecessor-version":[{"id":3757,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2935\/revisions\/3757"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2941"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2935"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2935"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}