{"id":2928,"date":"2023-01-07T10:52:45","date_gmt":"2023-01-07T16:52:45","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2928"},"modified":"2023-02-17T17:30:47","modified_gmt":"2023-02-17T23:30:47","slug":"amplite-universal-fluorimetric-mmp-activity-assay-kit-green-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-universal-fluorimetric-mmp-activity-assay-kit-green-fluorescence\/","title":{"rendered":"Amplite\u00ae Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*"},"content":{"rendered":"\n

Las metaloproteinasas de matriz (MMP) constituyen una familia de endopeptidasas dependientes de zinc que funcionan dentro de la matriz extracelular. Estas enzimas son responsables de la descomposici\u00f3n de los tejidos conectivos y son importantes en la remodelaci\u00f3n \u00f3sea, el ciclo menstrual y la reparaci\u00f3n del tejido da\u00f1ado. Mientras que la contribuci\u00f3n exacta de las MMP a ciertos procesos patol\u00f3gicos es dif\u00edcil de evaluar, las MMP parecen jugar un papel clave en el desarrollo de la artritis, as\u00ed como en la invasi\u00f3n y met\u00e1stasis del c\u00e1ncer. Las MMP tienden a tener m\u00faltiples sustratos, y la mayor\u00eda de los miembros de la familia tienen la capacidad de degradar diferentes tipos de col\u00e1geno junto con elastina, gelatina y fibronectina. <\/p>\n\n\n\n

Es bastante dif\u00edcil encontrar un sustrato que sea selectivo para una \u00fanica enzima MMP. Este kit est\u00e1 dise\u00f1ado para verificar la actividad general de una enzima MMP. Tambi\u00e9n se puede usar para detectar inhibidores de MMP cuando se usa una enzima MMP purificada. Tambi\u00e9n ofrecemos MMP enzim\u00e1ticas de alta actividad. <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-13510<\/td>Amplite\u00ae Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*<\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Cutoff<\/td>515 nm<\/td><\/tr>
Placa recomendada<\/td>Negra s\u00f3lida<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: MMP Green\u2122 Substrate<\/td>1 vial (60 \u00b5L)<\/td><\/tr>
Componente B: APMA, 4-Aminophenylmercuric Acetate<\/td>1 vial (20 \u00b5L, 1 M)<\/td><\/tr>
Componente C: Buffer de ensayo<\/td>1 botella (20 ml)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>494<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>515<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

  1. Soluci\u00f3n de trabajo de sustrato MMP Green\u2122:<\/em>
    Agregue 50 \u03bcl de sustrato MMP Green\u2122 (componente A) en 5 ml de buffer de ensayo (componente C) para obtener un volumen total de 5,05 ml de soluci\u00f3n de trabajo de sustrato MMP Green\u2122.<\/li>
  2. Diluci\u00f3n de MMP:<\/em>
    Diluya las MMP a una concentraci\u00f3n adecuada en el buffer de ensayo (componente C) si se usa MMP purificada. Nota: MMP debe activarse antes de su uso. Evite agitar la enzima en un v\u00f3rtex vigorosamente.<\/li>
  3. Diluci\u00f3n de inhibidores y compuestos:<\/em>
    Haga diluciones de inhibidores de MMP conocidos y compuestos de prueba seg\u00fan lo desee si est\u00e1 evaluando inhibidores de MMP.<\/li><\/ol>\n<\/div><\/div>\n\n\n\n
    <\/div>\n\n\n\n

    Imagen<\/mark><\/p>\n\n\n\n

    \"\"<\/figure>\n\n\n\n

    Figura 1.<\/strong> Detecci\u00f3n de actividad de MMP con el kit MMP fluorim\u00e9trico universal Amplite\u00ae. Las MMP activadas por APMA, 30 ng cada una, se mezclaron con sustrato MMP Green\u2122. La se\u00f1al de fluorescencia se control\u00f3 una hora despu\u00e9s de comenzar la reacci\u00f3n utilizando un lector de microplacas NOVOStar (BMG Labtech) con un juego de filtros de Ex\/Em = 490\/525 nm. La lectura de todos los pocillos se sustrajo de la lectura del control de sustrato, que contiene sustrato MMP Green\u2122 pero no MMP. El sustrato MMP Green\u2122 puede detectar la actividad de subnanogramos de todas las MMP (n=3).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Relacionados<\/mark><\/p>\n\n\n\n

    Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td><\/tr>
    Amplite\u00ae Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*<\/a><\/td>545<\/td>572<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    MMP Red\u2122 substrate<\/a><\/td><\/tr>
    MMP-3 Green\u2122 substrate<\/a><\/td><\/tr>
    Amplite\u00ae MMP-3 Activity Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
    MMP Green\u2122 substrate<\/a><\/td><\/tr>
    MMP-3 Green\u2122 substrate solution<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Ver todas las 26 bibliografias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

    Influence of Dinitrosyl Iron Complexes on the Activity of Enzymes, Indicators of Cardiovascular Diseases<\/a>
    Authors: <\/strong>Akentieva, Natalia and Sanina, Natalia and Gizatullin, Artur and Shkondina, Natalia and Andreeva, Anna and Shram, Stanislav and Aldoshin, Sergei
    Journal: <\/strong>(2022)<\/p>\n\n\n\n

    Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin<\/a>
    Authors: <\/strong>Seiser, Saskia and Janker, Lukas and Zila, Nina and Mildner, Michael and Rakita, Ana and Matiasek, Johannes and Bileck, Andrea and Gerner, Christopher and Paulitschke, Verena and Elbe-B{\\”u}rger, Adelheid
    Journal: <\/strong>Scientific Reports (2021): 1–13<\/p>\n\n\n\n

    Monodisperse drops templated by 3D-structured microparticles<\/a>
    Authors: <\/strong>Wu, Chueh-Yu and Ouyang, Mengxing and Wang, Bao and de Rutte, Joseph and Joo, Alexis and Jacobs, Matthew and Ha, Kyung and Bertozzi, Andrea L and Di Carlo, Dino
    Journal: <\/strong>Science advances (2020): eabb9023<\/p>\n\n\n\n

    Cloning and characteristic of MMP1 gene from Hyriopsis cumingii and collagen hydrolytic activity of its recombinant protein<\/a>
    Authors: <\/strong>Hu, Baoqing and Xiao, Jun and Yi, Peipei and Hu, Chenxi and Zhu, Mingxing and Yin, Shuyuan and Wen, Chungen and Wu, Jielian
    Journal: <\/strong>Gene (2019)<\/p>\n\n\n\n

    Osteopontin in the Pathogenesis of Aortic Dissection by the Enhancement of MMP Expressions<\/a>
    Authors: <\/strong>Fan, Fudong and Zhou, Qing and Xu, Zhenjun and Wang, Dongjin
    Journal: <\/strong>International Heart Journal (2019): 18–017<\/p>\n\n\n\n

    DACT2 epigenetic stimulator exerts dual efficacy for colorectal cancer prevention and treatment<\/a>
    Authors: <\/strong>Lu, Linlin and Wang, Ying and Ou, Rilan and Feng, Qian and Ji, Liyan and Zheng, Hongming and Guo, Yue and Qi, Xiaoxiao and Kong, Ah-Ng Tony and Liu, Zhongqiu
    Journal: <\/strong>Pharmacological Research (2018): 318–328<\/p>\n\n\n\n

    Nafamostat mesilate enhances the radiosensitivity and reduces the radiation-induced invasive ability of colorectal cancer cells
    Authors: <\/strong>Sugano, Hiroshi and Shirai, Yoshihiro and Horiuchi, Takashi and Saito, Nobuhiro and Shimada, Yohta and Eto, Ken and Uwagawa, Tadashi and Ohashi, Toya and Yanaga, Katsuhiko
    Journal: <\/strong>Cancers (2018): 386<\/p>\n\n\n\n

    Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3\u03b2\/snail-mediated pathway<\/a>
    Authors: <\/strong>Xiao, Ruyue and Yuan, Lan and He, Weijiang and Yang, Xiaoda
    Journal: <\/strong>Metallomics (2018)<\/p>\n\n\n\n

    RNA Interference Screening Identifies Clathrin-B and Cofilin-1 as Mediators of MT1-MMP in Endometrial Cancer<\/a>
    Authors: <\/strong>Baker, Tabari M and Waheed, Sana and Syed, Viqar
    Journal: <\/strong>Experimental cell research (2018)<\/p>\n\n\n\n

    DACT2 epigenetic stimulator exerts dual efficacy for colorectal cancer prevention and treatment
    Authors: <\/strong>Lu, Linlin and Wang, Ying and Ou, Rilan and Feng, Qian and Ji, Liyan and Zheng, Hongming and Guo, Yue and Qi, Xiaoxiao and Kong, Ah-Ng Tony and Liu, Zhongqiu
    Journal: <\/strong>Pharmacological research (2018): 318–328<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    EphA2 Induces Metastatic Growth Regulating Amoeboid Motility and Clonogenic Potential in Prostate Carcinoma Cells<\/a>
    Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3<\/a>
    EphA2 Induces Metastatic Growth Regulating Amoeboid Motility and Clonogenic Potential in Prostate Carcinoma Cells<\/a>
    Matrix Remodeling Maintains Embryonic Stem Cell Self-Renewal by Activating Stat3<\/a>
    EphA2 Induces Metastatic Growth Regulating Amoeboid Motility and Clonogenic Potential in Prostate Carcinoma Cells<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de actividad de MMP fluorim\u00e9trico universal, *Fluorescencia verde*. Este kit est\u00e1 dise\u00f1ado para verificar la actividad general de una enzima MMP. Tambi\u00e9n se puede usar para detectar inhibidores de MMP cuando se usa una enzima MMP purificada. <\/p>\n","protected":false},"featured_media":6034,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2928"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2928\/revisions"}],"predecessor-version":[{"id":3758,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2928\/revisions\/3758"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/6034"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2928"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2928"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}