{"id":2893,"date":"2023-01-06T16:06:46","date_gmt":"2023-01-06T22:06:46","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2893"},"modified":"2023-01-20T17:00:18","modified_gmt":"2023-01-20T23:00:18","slug":"amplite-fluorimetric-maleimide-quantitation-kit-green-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-fluorimetric-maleimide-quantitation-kit-green-fluorescence\/","title":{"rendered":"Amplite\u00ae Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*"},"content":{"rendered":"\n

Una variedad de reactivos  que entrecruzan con un grupo maleimida se usan ampliamente para cruzar prote\u00ednas con prote\u00ednas o prote\u00ednas con otras biomol\u00e9culas. Hay pocos reactivos o kits de ensayo disponibles para cuantificar el n\u00famero de grupos maleimida que se introducen en la primera prote\u00edna. Todos los kits comerciales tienen protocolos tediosos. Nuestro kit utiliza un tinte patentado que tiene una fluorescencia mejorada al reaccionar con una maleimida. <\/p>\n\n\n\n

El kit proporciona un m\u00e9todo fluorim\u00e9trico sensible de un solo paso con un limite de detecci\u00f3n hasta de 10 picomoles de maleimida en un volumen de ensayo de 100 \u00b5L (en una concentraci\u00f3n de 100 nM de maleimida). El ensayo es r\u00e1pido y robusto. Se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pozos o de 384 pozos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n. El kit proporciona un protocolo conveniente con todos los reactivos esenciales. Para la cuantificaci\u00f3n r\u00e1pida de una prote\u00edna del grupo maleimida, le recomendamos que utilice #5526. Para la cuantificaci\u00f3n de un grupo maleimida de nanopart\u00edculas, le recomendamos que utilice #5525.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-5523<\/td>Amplite\u00ae Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*<\/td>200 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Cutoff<\/td>515 nm<\/td><\/tr>
Placa recomendada<\/td>Negra s\u00f3lida<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Componentes<\/mark><\/p>\n\n\n\n

Componente A: Maleimide Green\u2122<\/td>1 vial<\/td><\/tr>
Componente B: Buffer de reacci\u00f3n<\/td>1 vial (500 \u00b5L)<\/td><\/tr>
Componente C: Buffer de ensayo<\/td>1 botella (25 mL)<\/td><\/tr>
Componente D: N-ethylmaleimide Standard<\/td>1 vial (10 mM, 50 \u00b5L )<\/td><\/tr>
Componente E: DMSO<\/td>1 vial (200 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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PREPARACION DE SOLUCIONES DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n. <\/em><\/p>\n\n\n\n

Soluci\u00f3n madre de Maleimide GreenTM (500X)<\/em>
Agregue 20 \u00b5L de DMSO (Componente E) en el vial de Maleimide GreenTM (Componente A) para preparar una soluci\u00f3n madre de Maleimide GreenTM 500X. Nota: 10 \u00b5L de soluci\u00f3n madre 500X Maleimide GreenTM es suficiente para una placa de 96 pocillos.<\/p>\n<\/div><\/div>\n\n\n\n

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PREPARACION DE SOLUCIONES ESTANDAR<\/p>\n\n\n\n

Para su conveniencia, utilice el Planificador de diluci\u00f3n en serie:
https:\/\/www.aatbio.com\/tools\/serial-dilution\/5523<\/a><\/p>\n\n\n\n

Est\u00e1ndar de N-etilaleimida<\/em>
Agregue 10 \u03bcL de est\u00e1ndar de N-etilmaleimida 10 mM (10 nmol\/\u03bcL) (componente D) a 990 \u03bcL de buffer de ensayo (componente C) para generar una soluci\u00f3n est\u00e1ndar de N-etilmaleimida 100 \u03bcM (100 pmol\/\u03bcL). Tome una soluci\u00f3n est\u00e1ndar de N-etilaleimida 100 \u03bcM (100 pmol\/\u03bcL) y diluya a 10 \u03bcM. A partir de una soluci\u00f3n est\u00e1ndar de 10 \u03bcM (MS7), realice diluciones en serie 1:2 para obtener est\u00e1ndares de N-etilmaleimida diluidos en serie (MS6-MS1) con buffer de ensayo (componente C).<\/p>\n<\/div><\/div>\n\n\n\n

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PREPARACION DE SOLUCIONES DE TRABAJO<\/p>\n\n\n\n

Agregue 10 \u03bcL de soluci\u00f3n madre 500X Maleimide GreenTM en 250 \u03bcL de buffer de reacci\u00f3n (componente B) y mezcle bien para hacer una mezcla de reacci\u00f3n 20X Maleimide. Incube la mezcla de reacci\u00f3n de Maleimida 20X a temperatura ambiente durante al menos 30 minutos, protegida de la luz. Nota: Es muy importante incubar la mezcla de reacci\u00f3n de Maleimida 20X durante al menos 30 minutos para maximizar la relaci\u00f3n se\u00f1al\/fondo. Nota: Deber\u00eda ver el color amarillo despu\u00e9s de agregar la soluci\u00f3n madre 500X Maleimide GreenTM en el buffer de reacci\u00f3n (componente B).<\/p>\n\n\n\n

Agregue toda la botella de mezcla de reacci\u00f3n de maleimida 20X en 5 ml de buffer de ensayo (componente C) y mezcle bien para obtener una soluci\u00f3n de trabajo de maleimida. Nota: Esta soluci\u00f3n de trabajo de Maleimida no es estable. Se debe usar dentro de la primera hora.<\/p>\n<\/div><\/div>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> La respuesta a la dosis de N-etilmaleimida se midi\u00f3 en una placa negra s\u00f3lida de 96 pozos con el kit de ensayo de cuantificaci\u00f3n de maleimida fluorim\u00e9trica Amplite\u2122 usando un lector de microplacas NOVOstar (BMG Labtech).<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td><\/tr>
Amplite\u00ae Colorimetric Maleimide Quantitation Kit<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric Aldehyde Quantitation Kit<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric Calcium Quantitation Kit *Red Fluorescence*<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric Glucose Quantitation Kit<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric \u03b1-Ketoglutarate Quantitation Kit<\/a><\/td><\/tr>
Amplite\u00ae Fluorimetric Protein Quantitation Kit *Orange Fluorescence*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Regulation of pancreatic cancer microenvironment by an intelligent gemcitabine@ nanogel system via in vitro 3D model for promoting therapeutic efficiency<\/a>
Authors: <\/strong>Chen, Di and Zhu, Xiaofei and Tao, Wanru and Kong, Yan and Huag, Yong and Zhang, Yajun and Liu, Ri and Jiang, Lingong and Tang, Ying and Yu, Haiyan and others,
Journal: <\/strong>Journal of Controlled Release (2020)<\/p>\n\n\n\n

Prote{\\’\\i}nas fluorescentes como sensibilizadores de emiss{\\~a}o em nanopart{\\’\\i}culas contendo Eu3+
Authors: <\/strong>Maturi, Fernando Eduardo
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Restricting the conformational freedom of the neuronal nitric-oxide synthase flavoprotein domain reveals impact on electron transfer and catalysis
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Conjugation with an inulin-chitosan adjuvant markedly improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10. 4 fusion protein<\/a>
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Study of Electron Transfer through the Reductase Domain of Neuronal Nitric Oxide Synthase and Development of Bacterial Nitric Oxide Synthase Inhibitors
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Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1<\/a>
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Rapid quantification of maleimide in bioconjuated samples using a novel colorimetric method (TECH2P. 761)<\/a>
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Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine<\/a>
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PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine<\/a>
Authors: <\/strong>Huang, Qingrui and Li, Dongxia and Kang, Aijun and An, Wenqi and Fan, Bei and Ma, Xiaowei and Ma, Guanghui and Su, Zhiguo and Hu, Tao
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CD133-targeted paclitaxel delivery inhibits local tumor recurrence in a mouse model of breast cancer<\/a>
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Journal: <\/strong>Journal of Controlled Release (2013): 280–287<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

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Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3
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Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides
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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de cuantificaci\u00f3n de maleimida fluorim\u00e9trica *Fluorescencia verde*, Amplite\u00ae. Nuestro kit utiliza un tinte patentado que tiene una fluorescencia mejorada al reaccionar con una maleimida. <\/p>\n","protected":false},"featured_media":2894,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2893"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2893\/revisions"}],"predecessor-version":[{"id":3761,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2893\/revisions\/3761"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2894"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2893"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2893"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}