{"id":2889,"date":"2023-01-06T15:14:31","date_gmt":"2023-01-06T21:14:31","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2889"},"modified":"2023-01-20T17:01:05","modified_gmt":"2023-01-20T23:01:05","slug":"amplite-colorimetric-maleimide-quantitation-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-colorimetric-maleimide-quantitation-kit\/","title":{"rendered":"Amplite\u00ae Colorimetric Maleimide Quantitation Kit"},"content":{"rendered":"\n

Las maleimidas se pueden analizar directamente espectrofotom\u00e9tricamente a 302 nm. Sin embargo, el peque\u00f1o coeficiente de extinci\u00f3n de 620 M-1cm-1 hace que este ensayo sea insensible y se complica a\u00fan m\u00e1s por la absorbancia de la prote\u00edna a la misma longitud de onda. Este kit de ensayo colorim\u00e9trico de maleimida cuantifica los grupos de maleimida haciendo reaccionar primero una muestra con una cantidad conocida de tiol presente en exceso y luego analizando el tiol restante sin reaccionar usando 4,4\\’-DTDP con un coeficiente de extinci\u00f3n molar de 19,800 M -1cm-1. La cantidad de maleimida se calcula como la diferencia entre la cantidad inicial de tiol y la cantidad de tiol sin reaccionar despu\u00e9s de la reacci\u00f3n completa de todos los grupos maleimida. <\/p>\n\n\n\n

Este ensayo espectrofotom\u00e9trico para la determinaci\u00f3n de grupos maleimida es un ensayo GSH inverso. Se aprovecha de la alta reactividad de los tioles de GSH con el resto maleimida. Se permite que la maleimida de la muestra forme un enlace tiosuccinimidil estable con GSH. Una vez completada la reacci\u00f3n de la muestra, se estima el exceso de GSH, es decir, los tioles restantes de GSH en la mezcla de reacci\u00f3n, usando 4,4\\’-DTDP. La cantidad de GSH que reacciona con la muestra se titula para determinar la cantidad de maleimida. Para una cuantificaci\u00f3n de maleimida m\u00e1s sensible, le recomendamos que utilice nuestro kit fluorim\u00e9trico catalogo 5523 que tiene una mayor sensibilidad.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-5525<\/td>Amplite\u00ae Colorimetric Maleimide Quantitation Kit<\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark> <\/p>\n\n\n\n

Absorbancia<\/td>324 nm<\/td><\/tr>
Placa recomendada<\/td>Fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Component A: MEA<\/td>1 vial<\/td><\/tr>
Component B: 4,4′-DTDP<\/td>1 vial <\/td><\/tr>
Component C: Buffer de ensayo<\/td>1 botella (50 mL)<\/td><\/tr>
Component D: DMSO<\/td>1 vial (1 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION DE SOLUCIONES DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

1. Soluci\u00f3n madre MEA (500X)<\/em><\/p>\n\n\n\n

Agregue 200 \u00b5L de agua destilada al vial de MEA (Componente A). Nota: 10 \u00b5L de la soluci\u00f3n madre 500X MEA es suficiente para 50 reacciones (0,5 mL\/reacci\u00f3n). La soluci\u00f3n madre de 500X MEA no utilizada debe dividirse en al\u00edcuotas de un solo uso, almacenarse a -20 o<\/sup>C y protegerse de la luz.<\/p>\n\n\n\n

2. Soluci\u00f3n madre de 4,4′-DTDP (50X)<\/em><\/p>\n\n\n\n

Agregue 1 mL de DMSO (Componente D) en el vial de 4,4′-DTDP (Componente B) y mezcle bien. Nota: 100 \u00b5L de la soluci\u00f3n madre 50X 4,4\u2019-DTDP es suficiente para 10 reacciones (0,5 mL\/reacci\u00f3n). La soluci\u00f3n madre de 50X 4,4′-DTDP no utilizada debe dividirse en al\u00edcuotas de un solo uso, almacenarse a -20 o<\/sup>C y protegerse de la luz.<\/p>\n<\/div><\/div>\n\n\n\n

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PREPARACION DE SOLUCIONES DE TRABAJO<\/p>\n\n\n\n

MEA Working Solution<\/em><\/p>\n\n\n\n

Agregue 10 \u03bcL de soluci\u00f3n madre de MEA (500X) en 5 mL de agua destilada y mezcle bien. Nota: La soluci\u00f3n de trabajo MEA no es estable. Recomendamos prepararla al momento antes de usar.<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Estructura qu\u00edmica del kit de cuantificaci\u00f3n de maleimida colorim\u00e9trica Amplite\u00ae.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

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<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

Perlecan-targeted nanoparticles for drug delivery to triple-negative breast cancer<\/a>
Authors: <\/strong>Khanna, Vidhi and Kalscheuer, Stephen and Kirtane, Ameya and Zhang, Wenqui and Panyam, Jayanth
Journal: <\/strong>Future Drug Discovery (2019)<\/p>\n\n\n\n

Conjugation with eight-arm PEG markedly improves the in vitro activity and prolongs the blood circulation of staphylokinase<\/a>
Authors: <\/strong>Qi, Fangbing and Hu, Chunyang and Yu, Weili and Hu, Tao
Journal: <\/strong>Bioconjugate chemistry (2018)<\/p>\n\n\n\n

Conjugation with an inulin-chitosan adjuvant markedly improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10. 4 fusion protein<\/a>
Authors: <\/strong>Yu, Weili and Hu, Tao
Journal: <\/strong>Molecular Pharmaceutics (2016)<\/p>\n\n\n\n

Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1<\/a>
Authors: <\/strong>Hattori, Tatsuya and Nakashima, Kenta and Marutani, Takayuki and Kiso, Yoshiaki and Nishi, Yoshisuke and Mukai, Hidehito
Journal: <\/strong>Biochemical and biophysical research communications (2015): 54–59<\/p>\n\n\n\n

Rapid quantification of maleimide in bioconjuated samples using a novel colorimetric method (TECH2P. 761)<\/a>
Authors: <\/strong>Liao, Jinfang and Guo, Haitao and Luo, Zhen and Zhao, Qin and Diwu, Jack
Journal: <\/strong>The Journal of Immunology (2015): 206–7<\/p>\n\n\n\n

Moderate PEGylation of the carrier protein improves the polysaccharide-specific immunogenicity of meningococcal group A polysaccharide conjugate vaccine<\/a>
Authors: <\/strong>Zhang, Tingting and Yu, Weili and Wang, Yanfei and Hu, Tao
Journal: <\/strong>Vaccine (2015): 3208–3214<\/p>\n\n\n\n

PEG as a spacer arm markedly increases the immunogenicity of meningococcal group Y polysaccharide conjugate vaccine<\/a>
Authors: <\/strong>Huang, Qingrui and Li, Dongxia and Kang, Aijun and An, Wenqi and Fan, Bei and Ma, Xiaowei and Ma, Guanghui and Su, Zhiguo and Hu, Tao
Journal: <\/strong>Journal of Controlled Release (2013): 382–389<\/p>\n\n\n\n

CD133-targeted paclitaxel delivery inhibits local tumor recurrence in a mouse model of breast cancer<\/a>
Authors: <\/strong>Swaminathan, Suresh Kumar and Roger, Emilie and Toti, Udaya and Niu, Lin and Ohlfest, John R and Panyam, Jayanth
Journal: <\/strong>Journal of Controlled Release (2013): 280–287<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Characterization of site-specific ScFv PEGylation for tumor-targeting pharmaceuticals
Authors: <\/strong>Natarajan A, Xiong CY, Albrecht H, DeNardo GL, DeNardo SJ.
Journal: <\/strong>Bioconjug Chem (2005): 113<\/p>\n\n\n\n

A new maleimide-bound acid-cleavable solid-support reagent for profiling phosphorylation
Authors: <\/strong>Chowdhury SM, Munske GR, Siems WF, Bruce JE.
Journal: <\/strong>Rapid Commun Mass Spectrom (2005): 899<\/p>\n\n\n\n

Quantitation of microparticles released from coated-platelets
Authors: <\/strong>Dale GL, Remenyi G, Friese P.
Journal: <\/strong>J Thromb Haemost (2005): 2081<\/p>\n\n\n\n

Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3
Authors: <\/strong>Chen J, Lu Z, Lawrence TS, Smith DE.
Journal: <\/strong>J Chromatogr B Analyt Technol Biomed Life Sci (2005): 161<\/p>\n\n\n\n

Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides
Authors: <\/strong>Li J, Xu Q, Cortes DM, Perozo E, Laskey A, Karlin A.
Journal: <\/strong>Proc Natl Acad Sci U S A (2002): 11605<\/p>\n\n\n\n

Resolution, detection, and characterization of redox conformers of human HSF1
Authors: <\/strong>Manalo DJ, Liu AY.
Journal: <\/strong>J Biol Chem (2001): 23554<\/p>\n\n\n\n

Site-directed mutation of conserved cysteine residues does not inactivate the Streptococcus pyogenes hyaluronan synthase
Authors: <\/strong>Heldermon CD, Tlapak-Simmons VL, Baggenstoss BA, Weigel PH.
Journal: <\/strong>Glycobiology (2001): 1017<\/p>\n\n\n\n

Quantitation of signal transduction
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Determination of S-nitrosoglutathione in human and rat plasma by high-performance liquid chromatography with fluorescence and ultraviolet absorbance detection after precolumn derivatization with o-phthalaldehyde
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Peroxynitrite modification of protein thiols: oxidation, nitrosylation, and S-glutathiolation of functionally important cysteine residue(s) in the sarcoplasmic reticulum Ca-ATPase
Authors: <\/strong>Viner RI, Williams TD, Schoneich C.
Journal: <\/strong>Biochemistry (1999): 12408<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de cuantificaci\u00f3n de maleimida colorim\u00e9trica, Amplite\u00ae. Este ensayo espectrofotom\u00e9trico para la determinaci\u00f3n de grupos maleimida es un ensayo GSH inverso. <\/p>\n","protected":false},"featured_media":2890,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2889"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":11,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2889\/revisions"}],"predecessor-version":[{"id":3762,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2889\/revisions\/3762"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2890"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2889"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}