{"id":2874,"date":"2023-01-06T12:08:37","date_gmt":"2023-01-06T18:08:37","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2874"},"modified":"2023-01-19T13:50:06","modified_gmt":"2023-01-19T19:50:06","slug":"cell-meter-colorimetric-wst-8-cell-quantification-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-colorimetric-wst-8-cell-quantification-kit\/","title":{"rendered":"Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit"},"content":{"rendered":"\n

Nuestros kits de ensayo Cell Meter\u2122 son un conjunto de herramientas para monitorear la viabilidad celular. Hay una variedad de par\u00e1metros que se pueden usar para monitorear la viabilidad celular. Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit utiliza sal de tetrazolio WST-8 soluble en agua para cuantificar el n\u00famero de c\u00e9lulas vivas. La sal de tetrazolio WST-8 soluble en agua produce un colorante de formaz\u00e1n naranja  tras la biorreducci\u00f3n en presencia de un transportador de electrones, 1-metoxi-5-metilfenazinio metlsulfato. El kit es conveniente y robusto con un formato de mezcla y lectura. La soluci\u00f3n WST-8 se agrega directamente a las celdas de prueba, no se requiere mezclar previamente los componentes. La sal de tetrazolio WST-8 se reduce mediante deshidrogenasas celulares a un producto de formaz\u00e1n naranja que es soluble en medio de cultivo tisular. La cantidad de formaz\u00e1n producido es directamente proporcional al n\u00famero de c\u00e9lulas vivas al monitorear el aumento de absorbancia a 460 nm. <\/p>\n\n\n\n

La excelente estabilidad y poca citotoxicidad de la soluci\u00f3n WST-8 hacen que el kit sea \u00fatil para los ensayos que requieren una incubaci\u00f3n prolongada (como de 24 a 48 horas). Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit proporciona un ensayo colorim\u00e9trico sensible para la determinaci\u00f3n del n\u00famero de c\u00e9lulas viables en los ensayos de proliferaci\u00f3n y citotoxicidad. La sensibilidad de detecci\u00f3n es mayor que cualquier otro ensayo basado en sal de tetrazolio, como MTT, XTT o MTS, etc. <\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22770<\/td>Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit<\/td>1000 ensayos<\/td><\/tr>
AAT-22771<\/td>Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit<\/td>5000 ensayos<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigerar (2-8 \u00b0C); Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark> <\/p>\n\n\n\n

Absorbancia<\/td>460 nm<\/td><\/tr>
Placa Recomendada<\/td>Fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/em><\/figcaption><\/figure>\n\n\n\n

Figura 1. <\/strong>El n\u00famero de c\u00e9lulas se determin\u00f3 con el kit de cuantificaci\u00f3n de c\u00e9lulas Cell Meter\u2122 Colorimetric WST-8. Se a\u00f1adieron c\u00e9lulas HeLa de 0 a 10.000 c\u00e9lulas\/pozo\/100 \u00b5l en una placa de 96 pozos de fondo transparente. La absorbancia se midi\u00f3 a 460 nm usando un lector SpectraMax (Molecular Devices).<\/p>\n\n\n\n

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Fig. 2<\/em><\/figcaption><\/figure>\n\n\n\n

Figura 2<\/strong>. Las pruebas de citotoxicidad de las c\u00e9lulas HeLa en respuesta a (A) SDS y (B) el tratamiento con estaurosporina se midieron con el kit de cuantificaci\u00f3n de c\u00e9lulas Cell Meter\u2122 Colorimetric WST-8. Se sembraron c\u00e9lulas HeLa a 10.000 c\u00e9lulas\/pocillo\/100 \u00b5l durante la noche en una placa de 96 pocillos de pared negra\/fondo transparente. Las c\u00e9lulas se trataron con SDS diluido en serie durante 2 horas o estaurosporina durante 4 horas. La absorbancia se midi\u00f3 a 460 nm utilizando un lector SpectraMax.<\/p>\n\n\n\n

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Fig. 3<\/em><\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> El n\u00famero de c\u00e9lulas se determin\u00f3 con el kit de cuantificaci\u00f3n de c\u00e9lulas Cell Meter\u2122 Colorimetric WST-8. Se a\u00f1adieron c\u00e9lulas Jurkat de 0 a 100.000 c\u00e9lulas\/pocillo\/100 \u00b5l en una placa de 96 pocillos de fondo transparente. La absorbancia se midi\u00f3 a 460 nm usando un lector SpectraMax (Molecular Devices).<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

MicroRNA-30a suppresses self-renewal and tumorigenicity of glioma stem cells by blocking the NT5E-dependent Akt signaling pathway<\/a>
Authors: <\/strong>Peng, Lilei and Ming, Yang and Zhang, Ling and Zhou, Jie and Xiang, Wei and Zeng, Shan and He, Haiping and Chen, Ligang
Journal: <\/strong>The FASEB Journal (2020): 5128–5143<\/p>\n\n\n\n

miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1<\/a>
Authors: <\/strong>Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: <\/strong>Molecular Medicine Reports (2017)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line
Authors: <\/strong>Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: <\/strong>Pharm Biol (2015): 849-54<\/p>\n\n\n\n

Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria
Authors: <\/strong>Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: <\/strong>J Microbiol Methods (2012): 160-6<\/p>\n\n\n\n

WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
Authors: <\/strong>Stoddart, M. J.
Journal: <\/strong>Methods Mol Biol (2011): 21-5<\/p>\n\n\n\n

monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates
Authors: <\/strong>Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: <\/strong>J Microbiol Methods (2007): 305-11<\/p>\n\n\n\n

Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency
Authors: <\/strong>Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: <\/strong>Acta Med Okayama (2006): 127-34<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Multicolor Analysis of Cell Proliferation Using a New Panel of CytoTell\u2122 Fluorescent Dyes<\/a>
Relative Brightness of Fluorescent Dyes<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit WST-8 de cuantificaci\u00f3n de c\u00e9lulas colorim\u00e9trico, Cell Meter\u2122 <\/p>\n","protected":false},"featured_media":2880,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2874"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2874\/revisions"}],"predecessor-version":[{"id":3623,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2874\/revisions\/3623"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2880"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2874"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2874"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}