{"id":2869,"date":"2023-01-06T11:42:55","date_gmt":"2023-01-06T17:42:55","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2869"},"modified":"2023-01-19T13:50:58","modified_gmt":"2023-01-19T19:50:58","slug":"amplite-colorimetric-aldehyde-quantitation-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-colorimetric-aldehyde-quantitation-kit\/","title":{"rendered":"Amplite\u00ae Colorimetric Aldehyde Quantitation Kit"},"content":{"rendered":"\n

La medici\u00f3n r\u00e1pida y precisa de los aldeh\u00eddos es una tarea importante para la investigaci\u00f3n biol\u00f3gica, la industria alimentaria, la investigaci\u00f3n qu\u00edmica y la vigilancia de la contaminaci\u00f3n ambiental. Hay pocos reactivos o kits de ensayo disponibles para cuantificar la cantidad de aldeh\u00eddos. La mayor\u00eda de los m\u00e9todos de prueba de aldeh\u00eddo existentes se basan en separaciones mediante HPLC-MS o GC-MS, tediosas y costosas. <\/p>\n\n\n\n

Nuestro kit de cuantificaci\u00f3n colorim\u00e9trica de aldeh\u00eddo utiliza un tinte patentado que genera un producto cromog\u00e9nico al reaccionar con un aldeh\u00eddo. El kit proporciona un m\u00e9todo colorim\u00e9trico sensible de un solo paso para detectar tan solo 1 nanomol de aldeh\u00eddo en un volumen de ensayo de 100 \u00b5L (10 \u00b5M; Figura 1). El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pozos o de 384 pozos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n sin necesidad de pasos de separaci\u00f3n. Su se\u00f1al se puede leer f\u00e1cilmente con un lector de microplacas de absorbancia a 550.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-10051<\/td>Amplite\u00ae Colorimetric Aldehyde Quantitation Kit<\/td>200 ensayos<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark> <\/p>\n\n\n\n

Absorbancia<\/td>550 nm<\/td><\/tr>
Placa Recomendada<\/td>Fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: AldeView\u2122 Yellow<\/td>2 botellas<\/td><\/tr>
Componente B: Solucion de Ensayo<\/td>1 botella (10ml)<\/td><\/tr>
Componente C: Est\u00e1ndar de aldeh\u00eddo<\/td>1 vial<\/td><\/tr>
Componente D: Buffer de diluci\u00f3n<\/td>1 botella (20 ml)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
PREPARACION DE SOLUCIONES DE STOCK<\/strong><\/h6>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n est\u00e1ndar de aldeh\u00eddo (10 mM):
    Agregue 1 ml de buffer de diluci\u00f3n (componente D) en el vial de est\u00e1ndar de aldeh\u00eddo (componente C) para preparar una soluci\u00f3n est\u00e1ndar de aldeh\u00eddo 10 mM.<\/li><\/ol>\n\n\n\n
    \n\n\n\n
    PREPARACION DE SOLUCIONES DE TRABAJO<\/strong><\/h6>\n\n\n\n

    Agregue 5 ml de soluci\u00f3n de ensayo (componente B) en una botella de AldeView\u2122 Yellow (componente A) y mezcalr bien. Nota: 5 ml de soluci\u00f3n de trabajo 2X AldeView\u2122 Yellow son suficientes para una placa. La soluci\u00f3n de trabajo 2X AldeView\u2122 Yellow no es estable y debe usarse dentro de las primeras 2 horas.<\/p>\n\n\n\n

    \n\n\n\n
    PREPARACION DE SOLUCIONE<\/strong> ESTANDAR<\/strong><\/h6>\n\n\n\n

    Para mayor comodidad, utilice el Planificador de diluci\u00f3n en serie: https:\/\/www.aatbio.com\/tools\/serial-dilution\/10051<\/a>
    Tome 10 mM de soluci\u00f3n est\u00e1ndar de aldeh\u00eddo y realice una diluci\u00f3n 1:10 para obtener una soluci\u00f3n est\u00e1ndar de aldeh\u00eddo 1000 uM (AS7). Luego realice diluciones en serie 1:3 para obtener las diluciones en serie restantes del est\u00e1ndar de aldeh\u00eddo (AS6 – AS1).<\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"
    Fig. 1<\/em><\/figcaption><\/figure>\n\n\n\n

    Figura 1.<\/strong> La respuesta a la dosis de aldeh\u00eddo se midi\u00f3 en una placa de 96 pozos de fondo blanco\/transparente con nuestro kit de ensayo de cuantificaci\u00f3n de aldeh\u00eddo colorim\u00e9trico Amplite\u00ae utilizando un lector de microplacas Spectrum Max (Molecular Devices).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Formatos Alternativos<\/mark><\/p>\n\n\n\n

    Amplite\u00ae Colorimetric Aldehyde Quantitation Kit<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

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    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Biological Activity of Peptide-conjugated Polyion Complex Matrices Consisting of Alginate and Chitosan<\/a>
    Authors: <\/strong>Fujimori, Chikara and Kumai, Jun and Nakamura, Kyotaro and Gu, Yingzi and Katagiri, Fumihiko and Hozumi, Kentaro and Kikkawa, Yamato and Nomizu, Motoyoshi
    Journal: <\/strong>Peptide Science (2016)<\/p>\n\n\n\n

    Integrated self-assembling drug delivery system possessing dual responsive and active targeting for orthotopic ovarian cancer theranostics<\/a>
    Authors: <\/strong>Lin, Chun-Jui and Kuan, Chen-Hsiang and Wang, Li-Wen and Wu, Hsi-Chin and Chen, Yunching and Chang, Chien-Wen and Huang, Rih-Yang and Wang, Tzu-Wei
    Journal: <\/strong>Biomaterials (2016): 12–26<\/p>\n\n\n\n

    Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice<\/a>
    Authors: <\/strong>Kumar, Sudhir and Wang, Jiang and Rani, Richa and G, undefined and hi, Ch and rashekhar R, undefined
    Journal: <\/strong>PloS one (2016): e0147864<\/p>\n\n\n\n

    Fiber-optic protease sensor based on the degradation of thin gelatin films<\/a>
    Authors: <\/strong>Schyrr, Bastien and Boder-Pasche, St\u00e9phanie and Ischer, R\u00e9al and Smajda, Rita and Voirin, Guy
    Journal: <\/strong>Sensing and Bio-Sensing Research (2015): 65–73<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Formaldehyde cross-linking of proteins to osteoclast differentiation factor promoter for the identification of biofunctional proteins
    Authors: <\/strong>Haghighi M., undefined
    Journal: <\/strong>Saudi Med J (2009): 472<\/p>\n\n\n\n

    Methods to reduce formaldehyde levels of cadavers
    Authors: <\/strong>Whitehead MC., undefined
    Journal: <\/strong>Clin Anat (2009): 421<\/p>\n\n\n\n

    Formaldehyde in China: production, consumption, exposure levels, and health effects
    Authors: <\/strong>Tang X, Bai Y, Duong A, Smith MT, Li L, Zhang L.
    Journal: <\/strong>Environ Int (2009): 1210<\/p>\n\n\n\n

    Urine formaldehyde level is inversely correlated to mini mental state examination scores in senile dementia
    Authors: <\/strong>Tong Z, Zhang J, Luo W, Wang W, Li F, Li H, Luo H, Lu J, Zhou J, Wan Y, He R.
    Journal: <\/strong>Neurobiol Aging. (2009)<\/p>\n\n\n\n

    Commentary: Mechanistic considerations for associations between formaldehyde exposure and nasopharyngeal carcinoma
    Authors: <\/strong>Thompson CM, Grafstrom RC.
    Journal: <\/strong>Environ Health (2009): 53<\/p>\n\n\n\n

    Formaldehyde dehydrogenase: Beyond phase I metabolism
    Authors: <\/strong>Thompson CM, Ceder R, Grafstrom RC.
    Journal: <\/strong>Toxicol Lett. (2009)<\/p>\n\n\n\n

    Toxicity of Methanol and Formaldehyde Towards Saccharomyces cerevisiae as Assessed by DNA Microarray Analysis
    Authors: <\/strong>Yasokawa D, Murata S, Iwahashi Y, Kitagawa E, Nakagawa R, Hashido T, Iwahashi H.
    Journal: <\/strong>Appl Biochem Biotechnol. (2009)<\/p>\n\n\n\n

    Measurement of atmospheric hydroxyacetone, glycolaldehyde, and formaldehyde
    Authors: <\/strong>Zhou X, Huang G, Civerolo K, Schwab J.
    Journal: <\/strong>Environ Sci Technol (2009): 2753<\/p>\n\n\n\n

    A temporal threshold for formaldehyde crosslinking and fixation
    Authors: <\/strong>Schmiedeberg L, Skene P, Deaton A, Bird A.
    Journal: <\/strong>PLoS One (2009): e4636<\/p>\n\n\n\n

    Genomic damages in peripheral blood lymphocytes and association with polymorphisms of three glutathione S-transferases in workers exposed to formaldehyde
    Authors: <\/strong>Jiang S, Yu L, Cheng J, Leng S, Dai Y, Zhang Y, Niu Y, Yan H, Qu W, Zhang C, Zhang K, Yang R, Zhou L, Zheng Y.
    Journal: <\/strong>Mutat Res. (2009)<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
    A<\/a>cetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
    A<\/a>meliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer<\/a>‘s <\/a>Diseas<\/a>e<\/a>
    An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with th<\/a>e A<\/a>lleviation of Depressive Symptoms in Overweight or Obese Adult<\/a>s<\/a>
    Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severit<\/a>y<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de cuantificaci\u00f3n colorim\u00e9trica de aldeh\u00eddos, Amplite\u00ae <\/p>\n","protected":false},"featured_media":2871,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2869"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2869\/revisions"}],"predecessor-version":[{"id":3624,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2869\/revisions\/3624"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2871"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2869"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2869"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}