{"id":2851,"date":"2023-01-05T17:29:32","date_gmt":"2023-01-05T23:29:32","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2851"},"modified":"2023-01-20T17:14:39","modified_gmt":"2023-01-20T23:14:39","slug":"cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kitdeep-red-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-fluorimetric-intracellular-total-ros-activity-assay-kitdeep-red-fluorescence\/","title":{"rendered":"Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluorescence*"},"content":{"rendered":"\n

Las especies reactivas de ox\u00edgeno (ROS) son subproductos naturales del metabolismo normal del ox\u00edgeno y juegan un papel importante en la se\u00f1alizaci\u00f3n celular. La acumulaci\u00f3n de ROS da como resultado un da\u00f1o significativo a las estructuras celulares. El papel del estr\u00e9s oxidativo en las enfermedades cardiovasculares, la diabetes, la osteoporosis, los accidentes cerebrovasculares, las enfermedades inflamatorias, una serie de enfermedades neurodegenerativas y el c\u00e1ncer ha sido bien establecido. La medici\u00f3n de ROS ayudar\u00e1 a determinar c\u00f3mo el estr\u00e9s oxidativo modula diversas v\u00edas intracelulares. <\/p>\n\n\n\n

El kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122 utiliza nuestro sensor patentado ROS Brite\u2122 670 para cuantificar ROS en c\u00e9lulas vivas. El ROS Brite\u2122 670, permeable a las c\u00e9lulas y no fluorescente, exhibe una fuerte se\u00f1al de fluorescencia al reaccionar con ROS. El sensor ROS Brite\u2122 670 est\u00e1 localizado en el citoplasma. La se\u00f1al de fluorescencia del sensor ROS Brite\u2122 670 se puede medir mediante microscop\u00eda de fluorescencia, im\u00e1genes de alto contenido, fluorometr\u00eda de microplaca o citometr\u00eda de flujo. <\/p>\n\n\n\n

El kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122 proporciona un ensayo fluorim\u00e9trico sensible de un solo paso para detectar ROS intracelulares (especialmente super\u00f3xido y radical hidroxilo) en c\u00e9lulas vivas dentro de 1 hora de incubaci\u00f3n. El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pocillos o de 384 pozos utilizando un lector de microplacas de fluorescencia o un microscopio de fluorescencia con filtro Cy5. <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22903<\/td>Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit<\/td>200 ensayos<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>650 nm<\/td><\/tr>
Emisi\u00f3n<\/td>675 nm<\/td><\/tr>
Corte<\/td>665 nm<\/td><\/tr>
Placa Recomendada<\/td>Paredes negras \/ fondo claro <\/td><\/tr>
Especificaciones Instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Cit\u00f3metro de flujo<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n Laser<\/td>640 nm <\/td><\/tr>
Emisi\u00f3n Filtro<\/td>660\/20 nm <\/td><\/tr>
Especificaciones Instrumento<\/td>Canal APC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Microscopio de Flourescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Juego de Filtros Cy5<\/td><\/tr>
Emisi\u00f3n<\/td>Juego de Filtros Cy5<\/td><\/tr>
Especificaciones Instrumento<\/td>Paredes negras \/ fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: ROS Brite\u2122 670<\/td>1 vial<\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 botella (20ml)<\/td><\/tr>
Componente C: DMSO<\/td>1 vial (100 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Preparaci\u00f3n de Soluci\u00f3n de Stock<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Soluci\u00f3n madre ROS Brite\u2122 670 (500X)<\/em>
Agregue 40 \u00b5L de DMSO (Componente C) en el vial de ROS Brite\u2122 670 (Componente A) y mezcle bien para preparar una soluci\u00f3n madre de 500X ROS Brite\u2122 670. Proteger de la luz.
Nota:<\/strong> Tenga en cuenta que 20 \u00b5L de soluci\u00f3n madre 500X ROS Brite\u2122 670 es suficiente para 1 placa. Para el cit\u00f3metro de flujo y para mayor comodidad, la soluci\u00f3n madre 500X ROS Brite\u2122 670 se puede diluir por 5 a 100X en DMSO. Para el almacenamiento, selle los tubos herm\u00e9ticamente.<\/p>\n\n\n\n

\n\n\n\n

Preparaci\u00f3n de Soluci\u00f3n de Trabajo<\/p>\n\n\n\n

Agregue 20 \u00b5l de soluci\u00f3n madre 500X ROS Brite\u2122 670 en 10 ml de buffer de ensayo (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo ROS Brite\u2122 670.
Nota:<\/strong> Esta soluci\u00f3n de trabajo ROS Brite\u2122 670 es estable durante al menos 2 horas a temperatura ambiente.<\/p>\n\n\n\n

\n\n\n\n

Preparaci\u00f3n de c\u00e9lulas<\/p>\n\n\n\n

Para guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

\"\"
Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Detecci\u00f3n de ROS en c\u00e9lulas HeLa con el kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122. Se sembraron 15.000 c\u00e9lulas  HeLa durante la noche  con \/90 \u00b5l pozo en una placa de 96 pocillos Costar de pared negra\/fondo transparente. Las c\u00e9lulas control no se trataron  o se trataron con 100 \u00b5M H2O2 a 1 mM o hidroper\u00f3xido de terc-butilo (TBHP) durante 30 minutos a 37 \u00b0C. Se a\u00f1adi\u00f3 la soluci\u00f3n de trabajo ROS Brite\u2122 670 (100 \u00b5l\/ pozo) y se incub\u00f3 en una incubadora a 37 \u00b0C con CO2 al 5 % durante 1 hora. La se\u00f1al de fluorescencia se control\u00f3 a Ex\/Em = 650\/675 nm (corte = 665 nm) con el modo de lectura inferior utilizando FlexStation (Molecular Devices).<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> Im\u00e1genes de c\u00e9lulas Hela te\u00f1idas con el kit de ensayo de actividad de ROS total intracelular fluorim\u00e9trico Cell Meter\u2122 en una placa de 96 pocillos de pared negra\/fondo transparente Costar. A: C\u00e9lulas control no tratadas. B: C\u00e9lulas tratadas con 100 \u00b5M de hidroper\u00f3xido de terc-butilo (TBHP) durante 30 minutos antes de la tinci\u00f3n.   <\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Detecci\u00f3n de ROS en c\u00e9lulas Jurkat. Las c\u00e9lulas Jurkat se trataron sin (verde) o con 100 \u00b5M hidroper\u00f3xido de terc-butilo (TBHP) (rojo) durante 30 minutos a 37 \u00b0C y luego se cargaron con ROS Brite\u2122 670 en una incubadora CO2 al 5 %, a 37 \u00b0C durante 1 hora. Las intensidades de fluorescencia se midieron con el canal APC utilizando un cit\u00f3metro de flujo (NovoCyte 3000, ACEA).<\/p>\n\n\n\n

<\/div>\n\n\n\n

Formatos Alternativos<\/mark><\/p>\n\n\n\n

Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*<\/a><\/td><\/tr>
C<\/a>ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescenc<\/a>e*<\/a><\/td><\/tr>
C<\/a>ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescenc<\/a>e*<\/a><\/td><\/tr>
C<\/a>ell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometr<\/a>y*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

Ver todas las 21 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Obstructive sleep apnea-increased DEC1 regulates systemic inflammation and oxidative stress that promotes development of pulmonary arterial hypertension<\/a>
Authors: <\/strong>Li, Xiaoming and Zhang, Xiang and Hou, Xiaozhi and Bing, Xin and Zhu, Fangyuan and Wu, Xinhao and Guo, Na and Zhao, Hui and Xu, Fenglei and Xia, Ming
Journal: <\/strong>Apoptosis (2022): 1–15<\/p>\n\n\n\n

ONC206 has anti-tumorigenic effects in human ovarian cancer cells and in a transgenic mouse model of high-grade serous ovarian cancer<\/a>
Authors: <\/strong>Tucker, Katherine and Yin, Yajie and Staley, Stuart-Allison and Zhao, Ziyi and Fang, Ziwei and Fan, Yali and Zhang, Xin and Suo, Hongyan and Sun, Wenchuan and Prabhu, Varun Vijay and others,
Journal: <\/strong>American Journal of Cancer Research (2022): 521<\/p>\n\n\n\n

Reversal of multidrug resistance by Fissistigma latifolium–derived chalconoid 2-hydroxy-4, 5, 6-trimethoxydihydrochalcone in cancer cell lines overexpressing human P-glycoprotein<\/a>
Authors: <\/strong>Teng, Yu-Ning and Hung, Chin-Chuan and Kao, Pei-Heng and Chang, Ying-Tzu and Lan, Yu-Hsuan
Journal: <\/strong>Biomedicine \\& Pharmacotherapy (2022): 113832<\/p>\n\n\n\n

Bcl-2 interacting protein 3 (BNIP3) promotes tumor growth in breast cancer under hypoxic conditions through an autophagy-dependent pathway<\/a>
Authors: <\/strong>Zhang, Guipu and Xu, Zhiyi and Yu, Minjing and Gao, Haiyan
Journal: <\/strong>Bioengineered (2022): 6280–6292<\/p>\n\n\n\n

The Abnormal Proliferation of Hepatocytes is Associated with MC-LR and C-Terminal Truncated HBX Synergistic Disturbance of the Redox Balance<\/a>
Authors: <\/strong>Cai, Dong-Mei and Mei, Fan-Biao and Zhang, Chao-Jun and An, San-Chun and Lv, Rui-Bo and Ren, Guan-Hua and Xiao, Chan-Chan and Long, Long and Huang, Tian-Ren and Deng, Wei
Journal: <\/strong>Journal of Hepatocellular Carcinoma (2022): 1229–1246<\/p>\n\n\n\n

Host Defense against Klebsiella pneumoniae Pneumonia Is Augmented by Lung-Derived Mesenchymal Stem Cells<\/a>
Authors: <\/strong>Rangasamy, Tirumalai and Ghimire, Laxman and Jin, Liliang and Le, John and Periasamy, Sivakumar and Paudel, Sagar and Cai, Shanshan and Jeyaseelan, Samithamby
Journal: <\/strong>The Journal of Immunology (2021): 1112–1127<\/p>\n\n\n\n

Low-power STED nanoscopy based on temporal and spatial modulation<\/a>
Authors: <\/strong>Wang, Luwei and Chen, Yue and Guo, Yong and Xie, Weixin and Yang, Zhigang and Weng, Xiaoyu and Yan, Wei and Qu, Junle
Journal: <\/strong>Nano Research (2021): 1–8<\/p>\n\n\n\n

Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions<\/a>
Authors: <\/strong>Shinohara, Yuta and Washio, Jumpei and Kobayashi, Yuri and Abiko, Yuki and Sasaki, Keiichi and Takahashi, Nobuhiro
Journal: <\/strong>Plos one (2021): e0254966<\/p>\n\n\n\n

Tyrosine kinase inhibitor conjugated quantum dots for non-small cell lung cancer (NSCLC) treatment<\/a>
Authors: <\/strong>Kulkarni, Nishant S and Parvathaneni, Vineela and Shukla, Snehal K and Barasa, Leonard and Perron, Jeanette C and Yoganathan, Sabesan and Muth, Aaron and Gupta, Vivek
Journal: <\/strong>European Journal of Pharmaceutical Sciences (2019)<\/p>\n\n\n\n

Anti-proliferation effect of blue light-emitting diodes against antibiotic-resistant Helicobacter pylori<\/a>
Authors: <\/strong>Ma, Jianwei and Hiratsuka, Takahiro and Etoh, Tsuyoshi and Akada, Junko and Fujishima, Hajime and Shiraishi, Norio and Yamaoka, Yoshio and Inomata, Masafumi
Journal: <\/strong>Journal of Gastroenterology and Hepatology (2018): 1492–1499<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

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Journal: <\/strong>Talanta (2009): 1219<\/p>\n\n\n\n

Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species
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Virion disruption by ozone-mediated reactive oxygen species
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The role of mitochondria in reactive oxygen species metabolism and signaling
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Sensitive determination of reactive oxygen species by chemiluminescence methods and their application to biological samples and health foods
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Reactive oxygen species and yeast apoptosis
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Measurement of reactive oxygen species in cells and mitochondria
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Role of reactive oxygen species in mediating hepatic ischemia-reperfusion injury and its therapeutic applications in liver transplantation
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Superoxide and derived reactive oxygen species in the regulation of hypoxia-inducible factors
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Reactive oxygen species and superoxide dismutases: role in joint diseases
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Journal: <\/strong>Joint Bone Spine (2007): 324<\/p>\n\n\n\n

<\/div>\n\n\n\n
Application notes (en Ingles)<\/strong><\/mark><\/h6>\n\n\n\n

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A Meta-Analysis of Common Calcium Indicators<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo para la actividad total de ROS intracelular fluorom\u00e9trico *florecencia rojo profundo*<\/p>\n","protected":false},"featured_media":2855,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2851"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":13,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2851\/revisions"}],"predecessor-version":[{"id":3764,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2851\/revisions\/3764"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2855"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2851"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2851"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}