{"id":2832,"date":"2023-01-05T16:39:35","date_gmt":"2023-01-05T22:39:35","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2832"},"modified":"2023-01-20T17:16:06","modified_gmt":"2023-01-20T23:16:06","slug":"phalloidin-ifluor-555-conjugate","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phalloidin-ifluor-555-conjugate\/","title":{"rendered":"Phalloidin-iFluor\u00ae 555 Conjugate"},"content":{"rendered":"\n

Este conjugado naranja fluorescente de faloidina (equivalente a la faloidina marcada con TRITC) se une selectivamente a las actinas F con una foto estabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina. <\/p>\n\n\n\n

Usados \u200b\u200ben concentraciones nanomolares, los derivados de faloidina son sondas convenientes para marcar, identificar y cuantificar actinas F en secciones de tejido permeabilizadas y fijadas con formaldeh\u00eddo, cultivos celulares o experimentos sin c\u00e9lulas. La faloidina se une a los filamentos de actina con mucha m\u00e1s fuerza que a los mon\u00f3meros de actina, lo que conduce a una disminuci\u00f3n de la constante de velocidad para la disociaci\u00f3n de las subunidades de actina de los extremos de los filamentos, lo que esencialmente estabiliza los filamentos de actina mediante la prevenci\u00f3n de la despolimerizaci\u00f3n de los filamentos. Adem\u00e1s, se encuentra que la faloidina inhibe la actividad de hidr\u00f3lisis de ATP de la actina F. La faloidina funciona de manera diferente en varias concentraciones en las c\u00e9lulas. Cuando se introduce en el citoplasma en bajas concentraciones, la faloidina recluta las formas menos polimerizadas de actina citopl\u00e1smica, as\u00ed como la filamina, en “islas” estables de pol\u00edmeros de actina agregados, pero no interfiere con las fibras de tensi\u00f3n, es decir, haces gruesos de microfilamentos. <\/p>\n\n\n\n

La propiedad de la faloidina es una herramienta \u00fatil para investigar la distribuci\u00f3n de F-actina en las c\u00e9lulas al marcar la faloidina con an\u00e1logos fluorescentes y usarlos para te\u00f1ir los filamentos de actina para microscop\u00eda \u00f3ptica. Los derivados fluorescentes de la faloidina han resultado ser de gran utilidad para localizar filamentos de actina en c\u00e9lulas vivas o fijas, as\u00ed como para visualizar filamentos de actina individuales in vitro. Los derivados de la faloidina fluorescente se han utilizado como una herramienta importante en el estudio de las redes de actina a alta resoluci\u00f3n. <\/p>\n\n\n\n

AAT Bioquest ofrece una variedad de derivados de faloidina fluorescente con diferentes colores para aplicaciones de im\u00e1genes multicolores.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-23119<\/td>Phalloidin-iFluor\u00ae 555 Conjugate<\/td>300 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento a largo plazo: Congelar a  < -15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso molecular <\/td>-1300<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (260 nm)<\/td>0.23<\/td><\/tr>
Factor de correci\u00f3n (280 nm)<\/td>0.14<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1000001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>557<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>570<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.641<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Imagen de fluorescencia de c\u00e9lulas HeLa fijadas con formaldeh\u00eddo al 4 % y luego te\u00f1idas con conjugado Phalloidin-iFluor\u00ae 555 (cat\u00e1logo 23119, rojo) y Nuclear Blue\u2122 DCS1 (cat\u00e1logo 17548, azul), respectivamente.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
Phalloidin-iFluor\u00ae 350 Conjugate<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
Phalloidin-iFluor\u00ae 405 Conjugate<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
Phalloidin-iFluor\u00ae 488 Conjugate<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
Phalloidin-iFluor\u00ae 514 Conjugate<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
Phalloidin-iFluor\u00ae 532 Conjugate<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
Phalloidin-iFluor\u00ae 594 Conjugate<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
Phalloidin-iFluor\u00ae 633 Conjugate<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
Phalloidin-iFluor\u00ae 647 Conjugate<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
Phalloidin-iFluor\u00ae 680 Conjugate<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n

Ver todas las 74 bibliograf\u00edas: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Extracellular Matrix Mimicking Dynamic Interpenetrating Network Hydrogel for Skin Tissue Engineering<\/a>
Authors: <\/strong>Wang, Weibin and Dai, Jiajia and Huang, Yvfeng and Li, Xiaomeng and Yang, Jianmin and Zheng, Yunquan and Shi, Xianai
Journal: <\/strong>Chemical Engineering Journal (2023): 141362<\/p>\n\n\n\n

Effects of Various Cell Surface Engineering Reactions on the Biological Behavior of Mammalian Cells<\/a>
Authors: <\/strong>Cai, Fengying and Ren, Yafeng and Dai, Jiajia and Yang, Jianmin and Shi, Xianai
Journal: <\/strong>Macromolecular Bioscience (2022): 2200379<\/p>\n\n\n\n

Calcium phosphate-adsorbable and acid-degradable carboxylated polyrotaxane consisting of $\\beta$-cyclodextrins suppresses osteoclast resorptive activity<\/a>
Authors: <\/strong>Yoshikawa, Yoshihiro and Tamura, Atsushi and Tsuda, Susumu and Domae, Eisuke and Zhang, Shunyao and Yui, Nobuhiko and Ikeo, Takashi and Yoshizawa, Tatsuya
Journal: <\/strong>Dental Materials Journal (2022): 2021–331<\/p>\n\n\n\n

Effect of mesoporous silica nanoparticles loaded with $\\alpha$-tomatine on HepG2 cancer cells studied in vitro<\/a>
Authors: <\/strong>Nepal, Bishal and Bhattarai, Jay K and Dhami, Kapur B and Nichols, Michael R and Stine, Keith J
Journal: <\/strong>Journal of Drug Delivery Science and Technology (2022): 104033<\/p>\n\n\n\n

p-Coumaric acid suppresses reactive oxygen species-induced senescence in nucleus pulposus cells<\/a>
Authors: <\/strong>Sheng, Kunkun and Li, Yan and Wang, Zhan and Hang, Kai and Ye, Zhaoming
Journal: <\/strong>Experimental and Therapeutic Medicine (2022): 1–10<\/p>\n\n\n\n

Assessment of Cellular Alignment and Proliferation on Cellulose Nanofibril (CNF) Films<\/a>
Authors: <\/strong>Roozbahani, Sahar
Journal: <\/strong>(2022)<\/p>\n\n\n\n

Development of milk exosome-based probes for biomedical imaging<\/a>
Authors: <\/strong>Gonz{\\’a}lez Guti{\\’e}rrez, Mar{\\’\\i}a Isabel
Journal: <\/strong>(2022)<\/p>\n\n\n\n

Senescence Induced by Nox4 Expressed Aberrantly in Osteoclast Precursors Accelerates Ostoeclastogenesis to Promote Osteoarthritis<\/a>
Authors: <\/strong>Yu, Bin and Qin, Hanjun and Liu, Xiaonan and Wang, Lei and He, Siying and Zhao, Xingqi and Yang, Jun and Wang, Shengnan and others,
Journal: <\/strong>(2022)<\/p>\n\n\n\n

Involvement of Aquaporin 1 in the Motility and in the Production of Fibrillin 1 and Type I Collagen of Cultured Human Dermal Fibroblasts<\/a>
Authors: <\/strong>Maeda, Kazuhisa and Yoshida, Shiori
Journal: <\/strong>Cosmetics (2022): 117<\/p>\n\n\n\n

Dual-labeled nanoparticles based on small extracellular vesicles for tumor detection<\/a>
Authors: <\/strong>Santos-Coquillat, Ana and Herreros-P{\\’e}rez, Desir{\\’e} and Samaniego, Rafael and Gonz{\\’a}lez, Mar{\\’\\i}a Isabel and Cuss{\\’o}, Lorena and Desco, Manuel and Salinas, Beatriz
Journal: <\/strong>Biology Direct (2022): 1–15<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: <\/strong>Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: <\/strong>Urology (2011): 970 e1<\/p>\n\n\n\n

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: <\/strong>Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: <\/strong>FEBS Lett (2011): 767<\/p>\n\n\n\n

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
Authors: <\/strong>An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.
Journal: <\/strong>Proc Natl Acad Sci U S A (2010): 20246<\/p>\n\n\n\n

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: <\/strong>Chazotte B., undefined
Journal: <\/strong>Cold Spring Harb Protoc (2010): pdb prot4947<\/p>\n\n\n\n

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 21<\/p>\n\n\n\n

Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers
Authors: <\/strong>Vig A, Dudas R, Kupi T, Orban J, Hild G, Lorinczy D, Nyitrai M.
Journal: <\/strong>J Therm Anal Calorim (2009): 721<\/p>\n\n\n\n

In vitro inhibition of OATP-mediated uptake of phalloidin using bile acid derivatives
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Vicens M, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 13<\/p>\n\n\n\n

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes
Authors: <\/strong>Luo H, Hallen-Adams HE, Walton JD.
Journal: <\/strong>J Biol Chem (2009): 18070<\/p>\n\n\n\n

Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations
Authors: <\/strong>Wollesen T, Loesel R, Wanninger A.
Journal: <\/strong>J Neurosci Methods (2009): 63<\/p>\n\n\n\n

Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis
Authors: <\/strong>Genikhovich G, Technau U.
Journal: <\/strong>Cold Spring Harb Protoc (2009): pdb prot5283<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Meta-Analysis of Common Calcium Indicators<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a><\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este conjugado naranja fluorescente de faloidina (equivalente a la faloidina marcada con TRITC) se une selectivamente a las actinas F con una foto estabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina. <\/p>\n","protected":false},"featured_media":2834,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2832"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2832\/revisions"}],"predecessor-version":[{"id":3765,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2832\/revisions\/3765"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2834"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2832"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2832"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}