{"id":2825,"date":"2023-01-05T14:15:19","date_gmt":"2023-01-05T20:15:19","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2825"},"modified":"2023-01-20T17:18:09","modified_gmt":"2023-01-20T23:18:09","slug":"phalloidin-ifluor-488-conjugate","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phalloidin-ifluor-488-conjugate\/","title":{"rendered":"Phalloidin-iFluor\u00ae 488 Conjugate"},"content":{"rendered":"\n

Este conjugado de faloidina fluorescente verde (equivalente a la faloidina marcada con Alexa Fluor\u00ae 488) se une selectivamente a las actinas F con una fotoestabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina. Usados \u200b\u200ben concentraciones nanomolares, los derivados de faloidina son sondas convenientes para marcar, identificar y cuantificar actinas F en secciones de tejido permeabilizadas y fijadas con formaldeh\u00eddo, cultivos celulares o experimentos sin c\u00e9lulas. <\/p>\n\n\n\n

La faloidina se une a los filamentos de actina con mucha m\u00e1s fuerza que a los mon\u00f3meros de actina, lo que conduce a una disminuci\u00f3n de la constante de velocidad para la disociaci\u00f3n de las subunidades de actina de los extremos de los filamentos, lo que esencialmente estabiliza los filamentos de actina mediante la prevenci\u00f3n de la despolimerizaci\u00f3n de los filamentos. Adem\u00e1s, se encuentra que la faloidina inhibe la actividad de hidr\u00f3lisis de ATP de la actina F. La faloidina funciona de manera diferente en varias concentraciones en las c\u00e9lulas. Cuando se introduce en el citoplasma en bajas concentraciones, la faloidina recluta las formas menos polimerizadas de actina citopl\u00e1smica, as\u00ed como la filamina, en “islas” estables de pol\u00edmeros de actina agregados, pero no interfiere con las fibras de tensi\u00f3n, es decir, haces gruesos de microfilamentos. <\/p>\n\n\n\n

La propiedad de la faloidina es una herramienta \u00fatil para investigar la distribuci\u00f3n de F-actina en las c\u00e9lulas al marcar la faloidina con an\u00e1logos fluorescentes y usarlos para te\u00f1ir los filamentos de actina para microscop\u00eda \u00f3ptica. Los derivados fluorescentes de la faloidina han resultado ser de gran utilidad para localizar filamentos de actina en c\u00e9lulas vivas o fijas, as\u00ed como para visualizar filamentos de actina individuales in vitro. Los derivados de la faloidina fluorescente se han utilizado como una herramienta importante en el estudio de las redes de actina a alta resoluci\u00f3n. AAT Bioquest ofrece una variedad de derivados de faloidina fluorescente con diferentes colores para aplicaciones de im\u00e1genes multicolores.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-23115<\/td>Phalloidin-iFluor\u00ae 488 Conjugate<\/td>300 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento a largo plazo: Congelar a  < -15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso molecular<\/td>-1100<\/td><\/tr>
DMSO<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (260 nm)<\/td>0.21<\/td><\/tr>
Factor de correci\u00f3n (280 nm)<\/td>0.11<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>750001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>491<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>516<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.91<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

<\/p>\n\n\n\n

\"\"
Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1. <\/strong>Im\u00e1genes de fluorescencia de c\u00e9lulas HeLa te\u00f1idas con el conjugado Phalloidin-iFluor\u00ae 488 utilizando un microscopio de fluorescencia con un juego de filtros FITC (verde). Las c\u00e9lulas se fijaron en formaldeh\u00eddo al 4 %, se co-marcaron con colorante mitocondrial MitoLite\u2122 Red FX600 (cat\u00e1logo 2677, Rojo) y Nuclear Blue\u2122 DCS1 (cat\u00e1logo 17548, Azul).<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2. <\/strong>C\u00e9lula de c\u00e1ncer de mama MDA-MB-231 creci\u00f3 durante 24 h. Las c\u00e9lulas se ti\u00f1eron con Phalloidin-iFluor 488 Conjugate (ATT Bioquest) siguiendo las instrucciones del fabricante. Las im\u00e1genes fueron adquiridas con un objetivo 63x\/1.4NA en un microscopio con focal de escaneo l\u00e1ser Zeiss por la Instalaci\u00f3n avanzada de bioimagen (ABIF) en McGill. Se muestra la Proyecci\u00f3n de m\u00e1xima intensidad de 19 im\u00e1genes con un espaciado de 0,2 um en Z.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
Phalloidin-iFluor\u00ae 350 Conjugate<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
Phalloidin-iFluor\u00ae 405 Conjugate<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
Phalloidin-iFluor\u00ae 514 Conjugate<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
Phalloidin-iFluor\u00ae 532 Conjugate<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
Phalloidin-iFluor\u00ae 555 Conjugate<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
Phalloidin-iFluor\u00ae 594 Conjugate<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
Phalloidin-iFluor\u00ae 633 Conjugate<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
Phalloidin-iFluor\u00ae 647 Conjugate<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
Phalloidin-iFluor\u00ae 680 Conjugate<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 184 bibliogr\u00e1fias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

M2-like macrophage-derived exosomes facilitate metastasis in non-small-cell lung cancer by delivering integrin $\\alpha$V$\\beta$3<\/a>
Authors: <\/strong>Huang, Lamei and Wang, Fang and Wang, Xueping and Su, Chaoyue and Wu, Shaocong and Yang, Chuan and Luo, Min and Zhang, Jianye and Fu, Liwu
Journal: <\/strong>MedComm (2023): e191<\/p>\n\n\n\n

Enhancing the paracrine effects of adipose stem cells using nanofiber-based meshes prepared by light-welding for accelerating wound healing<\/a>
Authors: <\/strong>Liu, Na and Zhou, Ziyi and Ning, Xuchao and Zhang, Xiaopei and Guo, Qingxia and Guo, Mingxia and Wang, Yuanfei and Wu, Tong
Journal: <\/strong>Materials \\& Design (2023): 111582<\/p>\n\n\n\n

PTEN regulates actin filaments to shape cell cortex architecture<\/a>
Authors: <\/strong>Ho, Kimberly and Patel, Suraj S and Sullivan, Eve and Chang, Alicia and Lin, Guang Han and Lv, Moqi and Fan, Xinyi and Naufal, ER and Shen, Wen H
Journal: <\/strong>(2023)<\/p>\n\n\n\n

Mechanical performance and cyocompatibility of PU\/PLCL nanofibrous electrospun scaffolds for skin regeneration<\/a>
Authors: <\/strong>Gao, Xiang and Wen, Meiling and Liu, Yang and Hou, Tian and An, Meiwen
Journal: <\/strong>Engineered Regeneration (2022)<\/p>\n\n\n\n

Splicing factor SF3B3, a NS5-binding protein, restricts ZIKV infection by targeting GCH1<\/a>
Authors: <\/strong>Chen, Tanxiu and Yang, Hao and Liu, Penghui and Hamiti, Moliduer and Zhang, Xintian and Xu, Yi and Quan, Wenqi and Zhang, Yong and Yu, Wenhai and Jiao, Li and others,
Journal: <\/strong>Virologica Sinica (2022)<\/p>\n\n\n\n

Platinum prodrug nanoparticles inhibiting tumor recurrence and metastasis by concurrent chemoradiotherapy<\/a>
Authors: <\/strong>Jiang, Wei and Wei, Lulu and Chen, Bing and Luo, Xingyu and Xu, Peipei and Cai, Jianfeng and Hu, Yong
Journal: <\/strong>Journal of Nanobiotechnology (2022): 1–16<\/p>\n\n\n\n

CD44 expression intensity marks colorectal cancer cell subpopulations with different extracellular vesicle release capacity<\/a>
Authors: <\/strong>Kelemen, Andrea and Carmi, Idan and Seress, Iv{\\’a}n and L{\\H{o}}rincz, P{\\’e}ter and T{\\”o}lgyes, Tam{\\’a}s and Dede, Krist{\\’o}f and Bursics, Attila and Buz{\\’a}s, Edit I and Wiener, Zolt{\\’a}n
Journal: <\/strong>International journal of molecular sciences (2022): 2180<\/p>\n\n\n\n

Multiple nerve cords connect the arms of octopuses, providing alternative paths for inter-arm signaling<\/a>
Authors: <\/strong>Kuuspalu, Adam and Cody, Samantha and Hale, Melina E
Journal: <\/strong>Current Biology (2022)<\/p>\n\n\n\n

Amino acids suppress macropinocytosis and promote release of CSF1 receptor in macrophages<\/a>
Authors: <\/strong>Mendel, Zachary I and Reynolds, Mack B and Abuaita, Basel H and O’Riordan, Mary X and Swanson, Joel A
Journal: <\/strong>Journal of cell science (2022): jcs259284<\/p>\n\n\n\n

Improved pharmacodynamics of epidermal growth factor via microneedles-based self-powered transcutaneous electrical stimulation<\/a>
Authors:\u00a0<\/strong>Yang, Yuan and Luo, Ruizeng and Chao, Shengyu and Xue, Jiangtao and Jiang, Dongjie and Feng, Yun Hao and Guo, Xin Dong and Luo, Dan and Zhang, Jiaping and Li, Zhou and others,
Journal:\u00a0<\/strong>Nature Communications\u00a0(2022):\u00a01–13<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 127 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: <\/strong>Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: <\/strong>Urology (2011): 970 e1<\/p>\n\n\n\n

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: <\/strong>Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: <\/strong>FEBS Lett (2011): 767<\/p>\n\n\n\n

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
Authors: <\/strong>An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.
Journal: <\/strong>Proc Natl Acad Sci U S A (2010): 20246<\/p>\n\n\n\n

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: <\/strong>Chazotte B., undefined
Journal: <\/strong>Cold Spring Harb Protoc (2010): pdb prot4947<\/p>\n\n\n\n

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 21<\/p>\n\n\n\n

Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers
Authors: <\/strong>Vig A, Dudas R, Kupi T, Orban J, Hild G, Lorinczy D, Nyitrai M.
Journal: <\/strong>J Therm Anal Calorim (2009): 721<\/p>\n\n\n\n

In vitro inhibition of OATP-mediated uptake of phalloidin using bile acid derivatives
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Vicens M, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 13<\/p>\n\n\n\n

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes
Authors: <\/strong>Luo H, Hallen-Adams HE, Walton JD.
Journal: <\/strong>J Biol Chem (2009): 18070<\/p>\n\n\n\n

Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations
Authors: <\/strong>Wollesen T, Loesel R, Wanninger A.
Journal: <\/strong>J Neurosci Methods (2009): 63<\/p>\n\n\n\n

Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis
Authors: <\/strong>Genikhovich G, Technau U.
Journal: <\/strong>Cold Spring Harb Protoc (2009): pdb prot5283<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

iFluor\u00ae Dye Selection Guide<\/a><\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este conjugado de faloidina fluorescente verde (equivalente a la faloidina marcada con Alexa Fluor\u00ae 488) se une selectivamente a las actinas F con una fotoestabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina.<\/p>\n","protected":false},"featured_media":2829,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2825"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2825\/revisions"}],"predecessor-version":[{"id":3767,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2825\/revisions\/3767"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2829"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2825"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2825"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}