{"id":2820,"date":"2023-01-05T13:32:01","date_gmt":"2023-01-05T19:32:01","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2820"},"modified":"2023-01-20T17:19:02","modified_gmt":"2023-01-20T23:19:02","slug":"phalloidin-ifluor-594-conjugate","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phalloidin-ifluor-594-conjugate\/","title":{"rendered":"Phalloidin-iFluor\u00ae 594 Conjugate"},"content":{"rendered":"\n

Este conjugado de faloidina fluorescente roja (equivalente a la faloidina marcada con Alexa Fluor\u00ae 594) se une selectivamente a las actinas F. Usados \u200b\u200ben concentraciones nanomolares, los derivados de faloidina son sondas convenientes para marcar, identificar y cuantificar actinas F en secciones de tejido permeabilizadas y fijadas con formaldeh\u00eddo, cultivos celulares o experimentos sin c\u00e9lulas. <\/p>\n\n\n\n

La faloidina se une a los filamentos de actina con mucha m\u00e1s fuerza que a los mon\u00f3meros de actina, lo que conduce a una disminuci\u00f3n de la constante de velocidad para la disociaci\u00f3n de las subunidades de actina de los extremos de los filamentos, lo que esencialmente estabiliza los filamentos de actina mediante la prevenci\u00f3n de la despolimerizaci\u00f3n de los filamentos. Adem\u00e1s, se encuentra que la faloidina inhibe la actividad de hidr\u00f3lisis de ATP de la actina F. La faloidina funciona de manera diferente en varias concentraciones en las c\u00e9lulas. Cuando se introduce en el citoplasma en bajas concentraciones, la faloidina recluta las formas menos polimerizadas de actina citopl\u00e1smica, as\u00ed como la filamina, en “islas” estables de pol\u00edmeros de actina agregados, pero no interfiere con las fibras de tensi\u00f3n, es decir, haces gruesos de microfilamentos. <\/p>\n\n\n\n

La propiedad de la faloidina es una herramienta \u00fatil para investigar la distribuci\u00f3n de F-actina en las c\u00e9lulas al marcar la faloidina con an\u00e1logos fluorescentes y usarlos para te\u00f1ir los filamentos de actina para microscop\u00eda \u00f3ptica. Los derivados fluorescentes de la faloidina han resultado ser de gran utilidad para localizar filamentos de actina en c\u00e9lulas vivas o fijas, as\u00ed como para visualizar filamentos de actina individuales in vitro. Los derivados de la faloidina fluorescente se han utilizado como una herramienta importante en el estudio de las redes de actina a alta resoluci\u00f3n. AAT Bioquest ofrece una variedad de derivados de faloidina fluorescente con diferentes colores para aplicaciones de im\u00e1genes multicolores.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-23122<\/td>Phalloidin-iFluor\u00ae 594 Conjugate<\/td>300 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento a largo plazo: Congelar a  < -15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso molecular<\/td>-1000<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Absorbancia (nm)<\/td>587<\/td><\/tr>
Factor de correci\u00f3n (260 nm)<\/td>0.05<\/td><\/tr>
Factor de correci\u00f3n (280 nm)<\/td>0.04<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1800001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>588<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>604<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.531<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

\"\"
Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Imagen de fluorescencia de c\u00e9lulas HeLa fijadas con formaldeh\u00eddo al 4 % y luego te\u00f1idas con Cell Navigator\u00ae F-Actin Labeling Kit *Red Fluorescence* en una placa Costar negra de 96 pozos. Las c\u00e9lulas se marcaron con Phalloidin-iFluor\u00ae 594 (n.\u00b0 de cat\u00e1logo 23122, rojo) y se ti\u00f1eron los n\u00facleos con DAPI (n.\u00b0 de cat\u00e1logo 17507, azul), respectivamente. El ret\u00edculo endopl\u00e1smico celular (RE) se ti\u00f1\u00f3 con ER Green\u2122 (Cat#22635, Green) antes de la fijaci\u00f3n.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
Phalloidin-iFluor\u00ae 350 Conjugate<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
Phalloidin-iFluor\u00ae 405 Conjugate<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
Phalloidin-iFluor\u00ae 488 Conjugate<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
Phalloidin-iFluor\u00ae 514 Conjugate<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
Phalloidin-iFluor\u00ae 532 Conjugate<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
Phalloidin-iFluor\u00ae 555 Conjugate<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
Phalloidin-iFluor\u00ae 633 Conjugate<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
Phalloidin-iFluor\u00ae 647 Conjugate<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
Phalloidin-iFluor\u00ae 680 Conjugate<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 92 bibliograf\u00edas : <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Different Cell Responses to Hinokitiol Treatment Result in Senescence or Apoptosis in Human Osteosarcoma Cell Lines<\/a>
Authors: <\/strong>Yang, Shun-Cheng and Chen, Hsuan-Ying and Chuang, Wan-Ling and Wang, Hui-Chun and Hsieh, Cheng-Pu and Huang, Yi-Fu
Journal: <\/strong>International Journal of Molecular Sciences (2022): 1632<\/p>\n\n\n\n

Blockade of the pro-fibrotic reaction mediated by the miR-143\/-145 cluster enhances the responses to targeted therapy in melanoma<\/a>
Authors: <\/strong>Diazzi, Serena and Baeri, Alberto and Fassy, Julien and Lecacheur, Margaux and Marin-Bejar, Oskar and Girard, Christophe A and Lefevre, Lauren and Lacoux, Caroline and Irondelle, Marie and Mounier, Carine and others,
Journal: <\/strong>EMBO molecular medicine (2022): e15295<\/p>\n\n\n\n

Reprogramming of ovarian aging epigenome by resveratrol<\/a>
Authors: <\/strong>Gou, Mo and Li, Jie and Yi, Lizhi and Li, Huiyu and Ye, Xiaoying and Wang, Huasong and Liu, Linlin and Sun, Baofa and Zhang, Song and Zhu, Zhengmao and others,
Journal: <\/strong>PNAS Nexus (2022)<\/p>\n\n\n\n

Calcium phosphate-adsorbable and acid-degradable carboxylated polyrotaxane consisting of $\\beta$-cyclodextrins suppresses osteoclast resorptive activity<\/a>
Authors: <\/strong>Yoshikawa, Yoshihiro and Tamura, Atsushi and Tsuda, Susumu and Domae, Eisuke and Zhang, Shunyao and Yui, Nobuhiko and Ikeo, Takashi and Yoshizawa, Tatsuya
Journal: <\/strong>Dental Materials Journal (2022): 2021–331<\/p>\n\n\n\n

Oral streptococci subvert the host innate immune response through hydrogen peroxide<\/a>
Authors: <\/strong>Tang, Yi Ling and Sim, Tiow Suan and Tan, Kai Soo
Journal: <\/strong>Scientific Reports (2022): 1–13<\/p>\n\n\n\n

Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1\/ATG14 pathway<\/a>
Authors: <\/strong>Zheng, Bingxin and Song, Keliang and Sun, Lingling and Gao, Yang and Qu, Yan and Ren, Chongmin and Yan, Peng and Chen, Wenfang and Guo, Wei and Zhou, Chuanli and others,
Journal: <\/strong>Cell \\& Bioscience (2022): 1–15<\/p>\n\n\n\n

Autophagy impairment is involved in midazolam-induced lipid droplet accumulation and consequent phagocytosis decrease in BV2 cells<\/a>
Authors: <\/strong>Zhu, Xiao-ling and Zhang, Hui-wen and Peng, Wen-jing and Gao, Shan and Yang, Zhi-lai and Zhang, Ji-qian and Liu, Xue-sheng
Journal: <\/strong>Biochemical and Biophysical Research Communications (2022)<\/p>\n\n\n\n

Construction of a T7 phage display nanobody library for bio-panning and identification of chicken dendritic cell-specific binding nanobodies<\/a>
Authors: <\/strong>Xu, Hai and Li, Ling and Deng, Bihua and Hong, Weiming and Li, Ruiting and Guo, Zijie and Hou, Jibo and Govinden, Roshini and Chenia, Hafizah Y
Journal: <\/strong>Scientific Reports (2022): 1–11<\/p>\n\n\n\n

Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis<\/a>
Authors: <\/strong>Mustonen, Anne-Mari and Lehmonen, Nina and Oikari, Sanna and Capra, Janne and Raekallio, Marja and Mykk{\\”a}nen, Anna and Paakkonen, Tommi and Rilla, Kirsi and Niemel{\\”a}, Tytti and Nieminen, Petteri
Journal: <\/strong>Scientific Reports (2022): 1–12<\/p>\n\n\n\n

Bioelectronic Screening Platform for Real-Time Monitoring of Tumour-derived Extracellular Vesicle-Induced Epithelial-to-Mesenchymal Transition<\/a>
Authors: <\/strong>Traberg, Walther and Uribe, Johana and Druet, Victor and Hama, Adel and Moysidou, Chrysanthi-Maria and Huerta, Miriam and McCoy, Reece and Hayward, Daniel and Savva, Achilleas and Genovese, Amaury and others,
Journal: <\/strong>(2022)<\/p>\n\n\n\n

<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 127 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: <\/strong>Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: <\/strong>Urology (2011): 970 e1<\/p>\n\n\n\n

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: <\/strong>Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: <\/strong>FEBS Lett (2011): 767<\/p>\n\n\n\n

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
Authors: <\/strong>An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.
Journal: <\/strong>Proc Natl Acad Sci U S A (2010): 20246<\/p>\n\n\n\n

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: <\/strong>Chazotte B., undefined
Journal: <\/strong>Cold Spring Harb Protoc (2010): pdb prot4947<\/p>\n\n\n\n

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 21<\/p>\n\n\n\n

Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers
Authors: <\/strong>Vig A, Dudas R, Kupi T, Orban J, Hild G, Lorinczy D, Nyitrai M.
Journal: <\/strong>J Therm Anal Calorim (2009): 721<\/p>\n\n\n\n

In vitro inhibition of OATP-mediated uptake of phalloidin using bile acid derivatives
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Vicens M, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 13<\/p>\n\n\n\n

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes
Authors: <\/strong>Luo H, Hallen-Adams HE, Walton JD.
Journal: <\/strong>J Biol Chem (2009): 18070<\/p>\n\n\n\n

Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations
Authors: <\/strong>Wollesen T, Loesel R, Wanninger A.
Journal: <\/strong>J Neurosci Methods (2009): 63<\/p>\n\n\n\n

Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis
Authors: <\/strong>Genikhovich G, Technau U.
Journal: <\/strong>Cold Spring Harb Protoc (2009): pdb prot5283<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Meta-Analysis of Common Calcium Indicators<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a><\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este conjugado de faloidina fluorescente roja (equivalente a la faloidina marcada con Alexa Fluor\u00ae 594) se une selectivamente a las actinas F. Ofrecemos una variedad de derivados de faloidina fluorescente con diferentes colores para aplicaciones de im\u00e1genes multicolores.<\/p>\n","protected":false},"featured_media":2822,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2820"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2820\/revisions"}],"predecessor-version":[{"id":3768,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2820\/revisions\/3768"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2822"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2820"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}