{"id":2815,"date":"2023-01-05T13:00:57","date_gmt":"2023-01-05T19:00:57","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2815"},"modified":"2023-01-20T17:20:11","modified_gmt":"2023-01-20T23:20:11","slug":"phalloidin-tetramethylrhodamine-conjugate","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phalloidin-tetramethylrhodamine-conjugate\/","title":{"rendered":"Phalloidin-Tetramethylrhodamine Conjugate"},"content":{"rendered":"\n

Este conjugado de faloidina naranja fluorescente (equivalente a la faloidina marcada con TRITC) se une selectivamente a las actinas F con una fotoestabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina. Usados \u200b\u200ben concentraciones nanomolares, los derivados de faloidina son sondas convenientes para marcar, identificar y cuantificar actinas F en secciones de tejido permeabilizadas y fijadas con formaldeh\u00eddo, cultivos celulares o experimentos sin c\u00e9lulas. <\/p>\n\n\n\n

La faloidina se une a los filamentos de actina con mucha m\u00e1s fuerza que a los mon\u00f3meros de actina, lo que conduce a una disminuci\u00f3n de la constante de velocidad para la disociaci\u00f3n de las subunidades de actina de los extremos de los filamentos, lo que esencialmente estabiliza los filamentos de actina mediante la prevenci\u00f3n de la despolimerizaci\u00f3n de los filamentos. Adem\u00e1s, se encuentra que la faloidina inhibe la actividad de hidr\u00f3lisis de ATP de la actina F. La faloidina funciona de manera diferente en varias concentraciones en las c\u00e9lulas. Cuando se introduce en el citoplasma en bajas concentraciones, la faloidina recluta las formas menos polimerizadas de actina citopl\u00e1smica, as\u00ed como la filamina, en “islas” estables de pol\u00edmeros de actina agregados, pero no interfiere con las fibras de tensi\u00f3n, es decir, haces gruesos de microfilamentos. <\/p>\n\n\n\n

La propiedad de la faloidina es una herramienta \u00fatil para investigar la distribuci\u00f3n de F-actina en las c\u00e9lulas al marcar la faloidina con an\u00e1logos fluorescentes y usarlos para te\u00f1ir los filamentos de actina para microscop\u00eda \u00f3ptica. Los derivados fluorescentes de la faloidina han resultado ser de gran utilidad para localizar filamentos de actina en c\u00e9lulas vivas o fijas, as\u00ed como para visualizar filamentos de actina individuales in vitro. Los derivados de la faloidina fluorescente se han utilizado como una herramienta importante en el estudio de las redes de actina a alta resoluci\u00f3n. AAT Bioquest ofrece una variedad de derivados de faloidina fluorescente con diferentes colores para aplicaciones de im\u00e1genes multicolores.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-23102<\/td>Phalloidin-Tetramethylrhodamine Conjugate<\/td>300 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento a largo plazo: Congelar a  < -15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso molecular<\/td>-1300<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (260 nm)<\/td>0.32<\/td><\/tr>
Factor de correci\u00f3n (280 nm)<\/td>0.178<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>90000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>552<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>578<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Estructura quimica de Conjugado de faloidina-tetrametilrodamina (Phalloidin-Tetramethylrhodamine Conjugate)<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Correction Factor (280 nm)<\/td><\/tr>
Phalloidin-AMCA Conjugate<\/a><\/td>346<\/td>434<\/td>19000<\/td>0.153<\/td><\/tr>
Phalloidin-Fluorescein Conjugate<\/a><\/td>498<\/td>517<\/td>800001<\/sup><\/td>0.275<\/td><\/tr>
Phalloidin-Biotin Conjugate<\/a><\/td>–<\/td>–<\/td>–<\/td>–<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 47 bibliogr\u00e1fias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

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Authors: <\/strong>Cheng, Dongliang and Wen, Zhiwei and Chen, Hui and Lin, Shiyuan and Zhang, Wei and Tang, Xin and Wu, Wei
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Journal: <\/strong>Biomedical Materials (2021): 065011<\/p>\n\n\n\n

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Journal: <\/strong>Bioactive Materials (2021)<\/p>\n\n\n\n

DNA biodots based targeted theranostic nanomedicine for the imaging and treatment of non-small cell lung cancer<\/a>
Authors: <\/strong>Jha, Abhishek and Viswanadh, Matte Kasi and Burande, Ankita Sanjay and Mehata, Abhishesh Kumar and Poddar, Suruchi and Yadav, Kanchan and Mahto, Sanjeev Kumar and Parmar, Avanish Singh and Muthu, Madaswamy S
Journal: <\/strong>International Journal of Biological Macromolecules (2020): 413–425<\/p>\n\n\n\n

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Authors: <\/strong>Chen, Lifeng and Mou, Shan and Hou, Jinfei and Fang, Huimin and Zeng, Yuyang and Sun, Jiaming and Wang, Zhenxing
Journal: <\/strong>Regenerative Biomaterials (2020)<\/p>\n\n\n\n

Formulation and in vivo efficacy study of cetuximab decorated targeted bioadhesive nanomedicine for non-small-cell lung cancer therapy<\/a>
Authors: <\/strong>Viswanadh, Matte Kasi and Vikas, and Jha, Abhishek and Reddy Adena, Sandeep Kumar and Mehata, Abhishesh Kumar and Priya, Vishnu and Neogi, Kaushik and Poddar, Suruchi and Mahto, Sanjeev Kumar and Muthu, Madaswamy S
Journal: <\/strong>Nanomedicine (2020): 2345–2367<\/p>\n\n\n\n

Plasmacytoid dendritic cells regulate host immune response to Citrobacter rodentium induced colitis in colon-draining lymph nodes<\/a>
Authors: <\/strong>P{\\”o}ysti, Sakari and Siloj{\\”a}rvi, Satu and Toivonen, Raine and H{\\”a}nninen, Arno
Journal: <\/strong>European Journal of Immunology (2020)<\/p>\n\n\n\n

OCT4 Suppresses Metastasis in Breast Cancer Cells Through Activation of STAT3 Signaling<\/a>
Authors: <\/strong>Jin, Xiangshu and Liu, Yafang and Qu, Huinan and Qi, Da and Wang, Xinqi and Lu, Yan and Dong, Yuan and Liang, Yingying and Jin, Qiu and Quan, Chengshi
Journal: <\/strong>(2020)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 127 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes
Authors: <\/strong>Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.
Journal: <\/strong>Urology (2011): 970 e1<\/p>\n\n\n\n

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin
Authors: <\/strong>Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.
Journal: <\/strong>FEBS Lett (2011): 767<\/p>\n\n\n\n

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation
Authors: <\/strong>An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.
Journal: <\/strong>Proc Natl Acad Sci U S A (2010): 20246<\/p>\n\n\n\n

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging
Authors: <\/strong>Chazotte B., undefined
Journal: <\/strong>Cold Spring Harb Protoc (2010): pdb prot4947<\/p>\n\n\n\n

Protective effect of bile acid derivatives in phalloidin-induced rat liver toxicity
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Hierro C, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 21<\/p>\n\n\n\n

Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers
Authors: <\/strong>Vig A, Dudas R, Kupi T, Orban J, Hild G, Lorinczy D, Nyitrai M.
Journal: <\/strong>J Therm Anal Calorim (2009): 721<\/p>\n\n\n\n

In vitro inhibition of OATP-mediated uptake of phalloidin using bile acid derivatives
Authors: <\/strong>Herraez E, Macias RI, Vazquez-Tato J, Vicens M, Monte MJ, Marin JJ.
Journal: <\/strong>Toxicol Appl Pharmacol (2009): 13<\/p>\n\n\n\n

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes
Authors: <\/strong>Luo H, Hallen-Adams HE, Walton JD.
Journal: <\/strong>J Biol Chem (2009): 18070<\/p>\n\n\n\n

Pygmy squids and giant brains: mapping the complex cephalopod CNS by phalloidin staining of vibratome sections and whole-mount preparations
Authors: <\/strong>Wollesen T, Loesel R, Wanninger A.
Journal: <\/strong>J Neurosci Methods (2009): 63<\/p>\n\n\n\n

Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis
Authors: <\/strong>Genikhovich G, Technau U.
Journal: <\/strong>Cold Spring Harb Protoc (2009): pdb prot5283<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A <\/a>Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Ce<\/a>lls<\/a>
Abbreviation of Common Chemical Compounds Related to Peptide<\/a>s<\/a>
Annexin <\/a>V<\/a>
B<\/a>right Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Diseas<\/a>e <\/a>Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

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<\/div>\n","protected":false},"excerpt":{"rendered":"

Conjugado de faloidina-tetrametilrodamina. Este conjugado de faloidina naranja fluorescente (equivalente a la faloidina marcada con TRITC) se une selectivamente a las actinas F con una fotoestabilidad mucho mayor que los conjugados de fluoresce\u00edna-faloidina.<\/p>\n","protected":false},"featured_media":2817,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2815"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2815\/revisions"}],"predecessor-version":[{"id":3769,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2815\/revisions\/3769"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2817"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2815"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2815"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}