{"id":2786,"date":"2023-01-03T15:20:50","date_gmt":"2023-01-03T21:20:50","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2786"},"modified":"2023-01-20T17:23:51","modified_gmt":"2023-01-20T23:23:51","slug":"phosphoworks-fluorimetric-pyrophosphate-assay-kit-blue-fluorescence-2","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phosphoworks-fluorimetric-pyrophosphate-assay-kit-blue-fluorescence-2\/","title":{"rendered":"PhosphoWorks\u2122 Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence*"},"content":{"rendered":"\n

El pirofosfato (PPi) se produce mediante una serie de reacciones bioqu\u00edmicas, como la hidr\u00f3lisis de ATP, las polimerizaciones de ADN y ARN, la formaci\u00f3n de AMP c\u00edclico por la enzima adenilato ciclasa y la activaci\u00f3n enzim\u00e1tica de los \u00e1cidos grasos para formar sus \u00e9steres de coenzima A. Nuestro kit de ensayo de pirofosfato PhosphoWroks\u2122 proporciona el m\u00e9todo espectrofotom\u00e9trico m\u00e1s s\u00f3lido para medir el pirofosfato. Este kit utiliza nuestro sensor de pirofosfato fluorog\u00e9nico patentado cuya intensidad de fluorescencia depende proporcionalmente de la concentraci\u00f3n de pirofosfato. <\/p>\n\n\n\n

Nuestro ensayo es mucho m\u00e1s f\u00e1cil y m\u00e1s robusto que los m\u00e9todos de pirofosfato de acoplamiento enzim\u00e1tico que requieren al menos dos enzimas para sus detecciones de pirofosfato. El kit proporciona todos los componentes esenciales para el ensayo de pirofosfato. Este kit se ha utilizado con \u00e9xito en la detecci\u00f3n de alto rendimiento (HTS). Consulte el descuento especial del paquete a granel de HTS para la detecci\u00f3n de m\u00e1s de 10 000 ensayos.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-21611<\/td>PhosphoWorks\u2122 Fluorimetric Pyrophosphate Assay Kit<\/td>200 ensayos<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>316 nm<\/td><\/tr>
Emisi\u00f3n<\/td>456 nm<\/td><\/tr>
Cutoff<\/td>420 nm<\/td><\/tr>
Placa Recomendada<\/td>Negro s\u00f3lido<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Component A: Buffer de ensayo<\/td>1 botella (25 mL)<\/td><\/tr>
Component B: Sensor PPi<\/td>1 vial (polvo liofilizado)<\/td><\/tr>
Component C: Estandar de pirofosfato<\/td>1 vial (1 mL, 50 mM)<\/td><\/tr>
Component D: DMSO<\/td>1 vial (200 \u00b5L)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Preparaci\u00f3n de Soluciones de Stock<\/strong><\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

  1. Soluci\u00f3n madre del sensor PPi (200X)<\/em>
    Agregue 50 \u00b5l de DMSO (componente D) en el vial del sensor PPi (componente B) para preparar una soluci\u00f3n madre del sensor PPi 200X. Proteger de la luz.
    Tenga en cuenta que 25 \u00b5L de la soluci\u00f3n madre del sensor PPi son suficientes para una placa de 96 pocillos.<\/li>
  2. Soluci\u00f3n est\u00e1ndar de pirofosfato (1 mM)<\/em>
    Agregue 10 \u03bcl de est\u00e1ndar de pirofosfato 50 mM (componente C) en 490 \u03bcl de ddH2O o buffer Hepes 50 mM (pH 7) para preparar una soluci\u00f3n est\u00e1ndar de pirofosfato 1 mM.<\/li><\/ol>\n\n\n\n
    \n\n\n\n

    Preparaci\u00f3n de Soluci\u00f3n Est\u00e1ndar<\/strong><\/p>\n\n\n\n

    Para mayor comodidad, utilice el Planificador de diluci\u00f3n en serie: https:\/\/www.aatbio.com\/tools\/serial-dilution\/21611<\/a><\/p>\n\n\n\n

    Est\u00e1ndar de pirofosfato<\/em><\/p>\n\n\n\n

    Agregue 50 \u03bcL de soluci\u00f3n est\u00e1ndar de pirofosfato 1 mM en 450 \u03bcL de ddH2O o buffer Hepes 50 mM para obtener una soluci\u00f3n est\u00e1ndar de pirofosfato 100 \u03bcM (PS7). Tome una soluci\u00f3n est\u00e1ndar de pirofosfato 100 \u03bcM y realice diluciones en serie 1:3 en ddH2O o buffer Hepes 50 mM para obtener est\u00e1ndares de pirofosfato diluidos en serie (PS6 – PS1).<\/p>\n\n\n\n

    \n\n\n\n

    Preparaci\u00f3n de Soluci\u00f3n de Trabajo<\/strong><\/p>\n\n\n\n

    Agregue 25 \u03bcL de soluci\u00f3n madre del sensor 200X PPi a 5 mL de buffer de ensayo (componente A) y mezcle bien para obtener una soluci\u00f3n de trabajo PPi.
    Nota<\/strong>: Debido a la alta sensibilidad de este ensayo a PPi, es importante utilizar reactivos y material de laboratorio sin PPi. DTT \u2265 1 mM aumentar\u00e1 el fondo, MgCl2 \u2265 2 mM disminuir\u00e1 la respuesta.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Imagen<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/figcaption><\/figure>\n\n\n\n

    Figura 1<\/strong>. Las respuestas a la dosis de pirofosfato, ATP y fosfato se midieron con el kit de ensayo de pirofosfato fluorom\u00e9trico Phospho Works\u2122 en una placa negra s\u00f3lida de 96 pocillos utilizando un lector de microplacas de fluorescencia.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Formato Alternativo<\/mark><\/p>\n\n\n\n

    PhosphoWorks\u2122 Fluorimetric Pyrophosphate Assay Kit *Enhanced Selectivity*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

    Productos Relacionados<\/mark><\/p>\n\n\n\n

    PhosphoWorks\u2122 Luminometric ATP Assay Kit *Steady Glow*<\/a><\/td><\/tr>
    PhosphoWorks\u2122 Luminometric ATP Assay Kit *Bright Glow*<\/a><\/td><\/tr>
    PhosphoWorks\u2122 Luminometric ATP Assay Kit *DTT-Free*<\/a><\/td><\/tr>
    PhosphoWorks\u2122 Colorimetric MESG Phosphate Assay Kit *UV absorption*<\/a><\/td><\/tr>
    PhosphoWorks\u2122 Colorimetric Phosphate Assay Kit *Blue Color*<\/a><\/td><\/tr>
    PhosphoWorks\u2122 Colorimetric ATP Assay Kit<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    AaTAS1 and AaMFS1 Genes for Biosynthesis or Efflux Transport of Tenuazonic Acid and Pathogenicity of Alternaria alternata<\/a>
    Authors: <\/strong>Sun, Fan and Cao, Xueqiang and Yu, Dianzhen and Hu, Dongqiang and Yan, Zheng and Fan, Yingying and Wang, Cheng and Wu, Aibo
    Journal: <\/strong>Molecular Plant-Microbe Interactions (2022): 416–427<\/p>\n\n\n\n

    RANKL Expression Is Increased in Circulating Mononuclear Cells of Patients with Calcific Aortic Stenosis<\/a>
    Authors: <\/strong>Rattazzi, Marcello and Faggin, Elisabetta and Bertacco, Elisa and Buso, Roberta and Puato, Massimo and Plebani, Mario and Zaninotto, Martina and Condotta, Davide and Zoppellaro, Giacomo and Pagliani, Leopoldo and others, undefined
    Journal: <\/strong>Journal of Cardiovascular Translational Research (2018): 1–10<\/p>\n\n\n\n

    Augmented fibroblast growth factor-23 secretion in bone locally contributes to impaired bone mineralization in chronic kidney disease in mice
    Authors: <\/strong>Andrukhova, Olena and Sch{\\”u}ler, Christiane and Bergow, Claudia and Petric, Alexandra and Erben, Reinhold G
    Journal: <\/strong>Frontiers in endocrinology (2018): 311<\/p>\n\n\n\n

    Biological studies and target engagement of the 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase (IspD)-targeting antimalarial agent (1 R, 3 S)-MMV008138 and analogs<\/a>
    Authors: <\/strong>Ghavami, Maryam and Merino, Emilio F and Yao, Zhong-Ke and Elahi, Rubayet and Simpson, Morgan E and Fern{\\’a}ndez-Murga, Maria L and Butler, Joshua H and Casasanta, Michael A and Krai, Priscilla M and Totrov, Maxim M and others,
    Journal: <\/strong>ACS infectious diseases (2017): 549–559<\/p>\n\n\n\n

    Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B<\/a>
    Authors: <\/strong>King, Dustin T and Wasney, Gregory A and Nosella, Michael and Fong, Anita and Strynadka, Natalie CJ
    Journal: <\/strong>Journal of Biological Chemistry (2017): 979–993<\/p>\n\n\n\n

    Biological studies and target-engagement of the 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)-targeting antimalarial agent (1R, 3S)-MMV008138 and analogs<\/a>
    Authors: <\/strong>Ghavami, Maryam and Merino, Emilio Fern and o , undefined and Yao, ZhongKe and Elahi, Rubayet and Simpson, Morgan and Fern, undefined and ez-Murga, Maria and Butler, Joshua Hayden and Casasanta, Michael and Krai, Priscilla and Totrov, Maxim and others, undefined
    Journal: <\/strong>ACS Infectious Diseases (2017)<\/p>\n\n\n\n

    It’s a NO for Bacterial Settlement: Nitric Oxide Regulated Biofilm Formation and Protein Expression<\/a>
    Authors: <\/strong>Xu, Yueming
    Journal: <\/strong>(2014)<\/p>\n\n\n\n

    Host-pathogen interaction and signaling molecule secretion are modified in the dpp3 knockout mutant of Candida lusitaniae<\/a>
    Authors: <\/strong>Sabra, Ayman and Bessoule, Jean-Jacques and Atanasova-Penichon, Vessela and No{\\”e}l, Thierry and Dementhon, Karine
    Journal: <\/strong>Infection and immunity (2014): 413–422<\/p>\n\n\n\n

    Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets<\/a>
    Authors: <\/strong>Wang, Maoqing and Yang, Xue and Ren, Lihong and Li, Songtao and He, Xuan and Wu, Xiaoyan and Liu, Tingting and Lin, Liqun and Li, Ying and Sun, Changhao
    Journal: <\/strong>Journal of proteome research (2014): 4131–4142<\/p>\n\n\n\n

    Host-Pathogen Interaction and Signaling Molecule Secretion Are Modified in the dpp3 Knockout Mutant of Candida lusitaniae<\/a>
    Authors: <\/strong>Sabra, Ayman and Bessoule, Jean-Jacques and Atanasova-Penichon, Vessela and Noel, Thierry and Dementhon, Karine
    Journal: <\/strong>Infection and immunity (2014): 413–422<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las 136 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

    Cell-surface-localized ATP detection with immobilized firefly luciferase
    Authors: <\/strong>Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E.
    Journal: <\/strong>Anal Biochem (2006): 61<\/p>\n\n\n\n

    Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs
    Authors: <\/strong>Campbell K, Swann K.
    Journal: <\/strong>Dev Biol (2006): 225<\/p>\n\n\n\n

    An efficient method for quantitative determination of cellular ATP synthetic activity
    Authors: <\/strong>Hara KY, Mori H.
    Journal: <\/strong>J Biomol Screen (2006): 310<\/p>\n\n\n\n

    Basic evaluation for new antimicrobial susceptibility testing of Mycobacterium leprae by bioluminescence assay (ATP method)
    Authors: <\/strong>Yamazaki T, Gidoh M, Matsuoka M.
    Journal: <\/strong>Nihon Hansenbyo Gakkai Zasshi (2006): 227<\/p>\n\n\n\n

    Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase
    Authors: <\/strong>Zamaraeva MV, Sabirov RZ, Maeno E, Ando-Akatsuka Y, Bessonova SV, Okada Y.
    Journal: <\/strong>Cell Death Differ (2005): 1390<\/p>\n\n\n\n

    Do rat cardiac myocytes release ATP on contraction
    Authors: <\/strong>Godecke S, Stumpe T, Schiller H, Schnittler HJ, Schrader J.
    Journal: <\/strong>Am J Physiol Cell Physiol (2005): C609<\/p>\n\n\n\n

    ATP bioluminescence assay for estimation of microbial populations of fresh-cut melon
    Authors: <\/strong>Ukuku DO, Sapers GM, Fett WF.
    Journal: <\/strong>J Food Prot (2005): 2427<\/p>\n\n\n\n

    Determination of ATP impurity in adenine dinucleotides
    Authors: <\/strong>Pojoga LH, Haghiac ML, Moose JE, Hilderman RH.
    Journal: <\/strong>Nucleosides Nucleotides Nucleic Acids (2004): 581<\/p>\n\n\n\n

    Identification of kinase inhibitors by an ATP depletion method
    Authors: <\/strong>Singh P, Harden BJ, Lillywhite BJ, Broad PM.
    Journal: <\/strong>Assay Drug Dev Technol (2004): 161<\/p>\n\n\n\n

    Detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay
    Authors: <\/strong>Arakawa H, Karasawa K, Igarashi T, Suzuki S, Goto N, Maeda M.
    Journal: <\/strong>Anal Biochem (2004): 296<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

    Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor<\/a>
    Selective Detection of Pyrophosphate Using a Fluorogenic Pyrophosphate Sensor<\/a>
    Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
    \u03b2-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic<\/a> <\/a>cytokines and metalloproteases in melanoma cell lines<\/a>
    B<\/a>io-inspired NADH regeneration by carbon nitride photocatalysis using diatom template<\/a>s<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Nuestro kit de ensayo de pirofosfato PhosphoWroks\u2122 proporciona el m\u00e9todo espectrofotom\u00e9trico m\u00e1s s\u00f3lido para medir el pirofosfato. Este kit se ha utilizado con \u00e9xito en la detecci\u00f3n de alto rendimiento (HTS).<\/p>\n","protected":false},"featured_media":2721,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2786"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2786\/revisions"}],"predecessor-version":[{"id":3773,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2786\/revisions\/3773"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2721"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2786"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2786"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}