{"id":2759,"date":"2023-01-03T12:16:23","date_gmt":"2023-01-03T18:16:23","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2759"},"modified":"2023-01-19T17:41:36","modified_gmt":"2023-01-19T23:41:36","slug":"texas-red-x-succinimidyl-ester-single-isomer","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/texas-red-x-succinimidyl-ester-single-isomer\/","title":{"rendered":"Texas Red-X, succinimidyl ester *Single isomer*\u00a0\u00a0\u00a0"},"content":{"rendered":"\n

Este reactivo con amina succinimidil \u00e9ster Texas Red\u00ae-X, es un reemplazo superior al Texas Red (Texas Red\u00ae es la marca registrada de Invitrogen). Tiene las mismas propiedades espectrales que Texas Red, pero es mucho m\u00e1s f\u00e1cil de conjugar con una biomol\u00e9cula con un rendimiento de conjugaci\u00f3n mucho mayor. Se puede utilizar para crear bioconjugados fluorescentes de color rojo brillante con un m\u00e1ximo de excitaci\u00f3n\/emisi\u00f3n de ~595\/615 nm. En comparaci\u00f3n con Texas Red\u00ae, este colorante reactivo contiene un espaciador de aminohexanoilo (“X”) adicional de siete \u00e1tomos entre el fluor\u00f3foro y el grupo \u00e9ster de succinimidilo. Este espaciador ayuda a separar el fluor\u00f3foro de su punto de uni\u00f3n, reduciendo potencialmente la interacci\u00f3n del fluor\u00f3foro con la biomol\u00e9cula a la que est\u00e1 conjugado. En comparaci\u00f3n con los is\u00f3meros mixtos (#474), este producto es el is\u00f3mero 5 \u00fanico, lo que brinda resultados m\u00e1s reproducibles.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-474<\/td>Texas Red-X, succinimidyl ester *Single isomer* *178623-11-5*<\/td>5mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>816.94<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.23<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.36<\/td><\/tr>
Factor de correcci\u00f3n (565 nm)<\/td>0.4472<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1160001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>586<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>603<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.932<\/sup>, 0.351<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Agua, 2<\/sup> Etanol, <\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de Texas Red-X, succinimidil \u00e9ster Is\u00f3mero \u00fanico<\/em> 178623-11-5<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>122.408 \u00b5L<\/td>612.04 \u00b5L<\/td>1.224 mL<\/td>6.12 mL<\/td>12.241 mL<\/td><\/tr>
5 mM<\/strong><\/td>24.482 \u00b5L<\/td>122.408 \u00b5L<\/td>244.816 \u00b5L<\/td>1.224 mL<\/td>2.448 mL<\/td><\/tr>
10 mM<\/strong><\/td>12.241 \u00b5L<\/td>61.204 \u00b5L<\/td>122.408 \u00b5L<\/td>612.04 \u00b5L<\/td>1.224 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Figura 1<\/strong>. Estructura quimica de Texas Red-X, succinimidyl ester *Single isomer* *CAS 178623-11-5*<\/p>\n\n\n\n

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Texas Red\u00ae hydrazide *Single Isom<\/a>er*<\/a><\/td><\/tr>
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Texas Red\u00ae-streptavidin conjugate<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Glycosylation of a key cubilin Asn residue results in reduced binding to albumin<\/a>
Authors: <\/strong>Yadav, Shiv Pratap Singh and Yu, Aiying and Zhao, Jingfu and Singh, Jasdeep and Kakkar, Saloni and Chakraborty, Srinivas and Mechref, Yehia and Molitoris, Bruce and Wagner, Mark C
Journal: <\/strong>Journal of Biological Chemistry (2022): 102371<\/p>\n\n\n\n

A Novel Active Peptide from Rapana venosa Protects Against Gentamicin-Induced Sensory Hair Cell Loss in Zebrafish<\/a>
Authors: <\/strong>Gao, Yan and Zhang, Shanshan and Wan, Jiahui and Wang, Rongchun and Ba, Shuaikang and Ji, Xiuna and Sun, Chen and Liu, Kechun
Journal: <\/strong>International Journal of Peptide Research and Therapeutics (2020): 1–9<\/p>\n\n\n\n

A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos<\/a>
Authors: <\/strong>Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: <\/strong>Journal of Applied Toxicology (2020)<\/p>\n\n\n\n

Mechanism of How Carbamylation Reduces Albumin Binding to FcRn contributing to increased vascular clearance.<\/a>
Authors: <\/strong>Yadav, Shiv Pratap S and Sandoval, Ruben and Zhao, Jingfu and Huang, Yifan and Wang, Exing and Kumar, Sudhanshu and Campos-Bilderback, Silvia B and Rhodes, George J and Mechref, Yehia and Molitoris, Bruce A and others,
Journal: <\/strong>American Journal of Physiology-Renal Physiology (2020)<\/p>\n\n\n\n

Alternative enzyme protection assay to overcome the drawbacks of the gentamicin protection assay for measuring entry and intracellular survival of staphylococci
Authors: <\/strong>Kim, Jin-Hahn and Chaurasia, Akhilesh Kumar and Batool, Nayab and Ko, Kwan Soo and Kim, Kyeong Kyu
Journal: <\/strong>Infection and immunity (2019): e00119–19<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: <\/strong>Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: <\/strong>Photochem Photobiol (2005): 682<\/p>\n\n\n\n

A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
Authors: <\/strong>Garcia-Fuentes M, Prego C, Torres D, Alonso MJ.
Journal: <\/strong>Eur J Pharm Sci (2005): 133<\/p>\n\n\n\n

Bead-based cellular analysis, sorting and multiplexing
Authors: <\/strong>Sanchez-Martin RM, Muzerelle M, Chitkul N, How SE, Mittoo S, Bradley M.
Journal: <\/strong>Chembiochem (2005): 1341<\/p>\n\n\n\n

Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: <\/strong>Leader JP, O’Donnell MJ.
Journal: <\/strong>J Exp Biol (2005): 4363<\/p>\n\n\n\n

Purification and fluorescent labeling of the human serotonin transporter
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Journal: <\/strong>Biochemistry (2005): 3494<\/p>\n\n\n\n

Budding dynamics of multicomponent tubular vesicles
Authors: <\/strong>Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: <\/strong>J Am Chem Soc (2005): 17996<\/p>\n\n\n\n

Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
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Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
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Journal: <\/strong>Biophys J (2005): 3455<\/p>\n\n\n\n

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
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Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors:\u00a0<\/strong>Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal:\u00a0<\/strong>Shock\u00a0(2003):\u00a0582<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este reactivo con amina succinimidil \u00e9ster Texas Red\u00ae-X, es un reemplazo superior al Texas Red\u00ae (marca registrada de Invitrogen). <\/p>\n","protected":false},"featured_media":2761,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2759"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2759\/revisions"}],"predecessor-version":[{"id":3645,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2759\/revisions\/3645"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2761"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2759"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}