{"id":2740,"date":"2023-01-03T10:54:57","date_gmt":"2023-01-03T16:54:57","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2740"},"modified":"2023-03-10T21:17:06","modified_gmt":"2023-03-11T03:17:06","slug":"mycolight-green-jj98-5-mm-in-dmso","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/mycolight-green-jj98-5-mm-in-dmso\/","title":{"rendered":"MycoLight\u2122 Green JJ98 *5 mM in DMSO*"},"content":{"rendered":"\n

MycoLight\u2122 Green JJ98 es una tinci\u00f3n cromos\u00f3mica y nuclear verde fluorescente que es permeable a las membranas celulares tanto procari\u00f3ticas como eucari\u00f3ticas. MycoLight\u2122 Green JJ98 tiene una alta afinidad por el ADN y exhibe una fluorescencia mejorada al unirse con un m\u00e1ximo de excitaci\u00f3n cercano a la l\u00ednea de l\u00e1ser de arg\u00f3n de 488 nm y un m\u00e1ximo de emisi\u00f3n de fluorescencia a ~500 nm. MycoLight\u2122 Green JJ98 es especialmente \u00fatil como contratinci\u00f3n nuclear para ensayos bacterianos, ya que ti\u00f1e bacterias grampositivas y gramnegativas vivas y muertas. Es un excelente reemplazo para SYTO\u00ae 9 (SYTO\u00ae es la marca registrada de Invitrogen).<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-24000<\/td>MycoLight\u2122 Green JJ98 *5 mM in DMSO*<\/td>100 uL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar (< -15 \u00b0C); Minimizar la exposici\u00f3n a la luz <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de Flujo<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n de laser<\/td>488 nm <\/td><\/tr>
Filtro de emisi\u00f3n<\/td>530\/30 nm <\/td><\/tr>
Especificaciones instrumento<\/td>FITC channel<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Microscopio de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Juego de filtros FITC<\/td><\/tr>
Emisi\u00f3n<\/td>Juego de filtros FITC<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular<\/td>534<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Exitaci\u00f3n (nm)<\/td>482<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>512<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de MycoLight\u2122 Green JJ98 *5 mM en DMSO* a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>187.266 \u00b5L<\/td>936.33 \u00b5L<\/td>1.873 mL<\/td>9.363 mL<\/td>18.727 mL<\/td><\/tr>
5 mM<\/strong><\/td>37.453 \u00b5L<\/td>187.266 \u00b5L<\/td>374.532 \u00b5L<\/td>1.873 mL<\/td>3.745 mL<\/td><\/tr>
10 mM<\/strong><\/td>18.727 \u00b5L<\/td>93.633 \u00b5L<\/td>187.266 \u00b5L<\/td>936.33 \u00b5L<\/td>1.873 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1<\/strong>. E.Coli se ti\u00f1\u00f3 con 5 uM de MycoLight\u2122 Green JJ98 durante 30 minutos y se tomaron im\u00e1genes con el canal FITC.<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> E.Coli se ti\u00f1\u00f3 con 5 uM de MycoLight\u2122 Green JJ98 durante 30 minutos y se tomaron im\u00e1genes con el canal FITC.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

M<\/a>ycoLight\u2122 Green JJ98 *5 mM in DMSO<\/a>*<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Green JJ99 *5 mM in DMS<\/a>O*<\/a><\/td><\/tr>
My<\/a>coLight\u2122 Ratiometric Bacterial Membrane Potential Kit *Red\/Green Fluorescen<\/a>ce*<\/a><\/td><\/tr>
My<\/a>coLight\u2122 Fluorimetric CTC Live Bacteria Quantification K<\/a>it<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Flow Cytometric Live Bacteria Assay K<\/a>it<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Live Bacteria Fluorescence Imaging K<\/a>it<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Red JJ94 *2.5 mM in DMS<\/a>O*<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Fluorescence Live\/Dead Bacterial Imaging K<\/a>it<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Rapid Fluorescence Bacterial Gram Stain K<\/a>it<\/a><\/td><\/tr>
M<\/a>ycoLight\u2122 Rapid Fluorescence Gram-Positive Bacteria Staining K<\/a>it<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Referencias<\/mark><\/p>\n\n\n\n

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: <\/strong>Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: <\/strong>Am J Trop Med Hyg (2014): 306<\/p>\n\n\n\n

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: <\/strong>Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: <\/strong>Clin Lab (2013): 795<\/p>\n\n\n\n

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: <\/strong>Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: <\/strong>BMC Res Notes (2012): 437<\/p>\n\n\n\n

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: <\/strong>Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: <\/strong>Mol Cell Probes (2012): 121<\/p>\n\n\n\n

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: <\/strong>Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: <\/strong>Methods Mol Biol (2011): 81<\/p>\n\n\n\n

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: <\/strong>Eischeid AC., undefined
Journal: <\/strong>BMC Res Notes (2011): 263<\/p>\n\n\n\n

Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
Authors: <\/strong>Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: <\/strong>Cytometry A (2010): 294<\/p>\n\n\n\n

Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9
Authors: <\/strong>Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: <\/strong>Folia Microbiol (Praha) (2010): 502<\/p>\n\n\n\n

Validation of SYTO 9\/propidium iodide uptake for rapid detection of viable but noncultivable Legionella pneumophila
Authors: <\/strong>Giao MS, Wilks SA, Azevedo NF, Vieira MJ, Keevil CW.
Journal: <\/strong>Microb Ecol (2009): 56<\/p>\n\n\n\n

Quantitative measurement of Plasmodium-infected erythrocytes in murine models of malaria by flow cytometry using bidimensional assessment of SYTO-16 fluorescence
Authors: <\/strong>Jimenez-Diaz MB, Mulet T, Gomez V, Viera S, Alvarez A, Garuti H, Vazquez Y, Fern and ez A, Ibanez J, Jimenez M, Gargallo-Viola D, Angulo-Barturen I.
Journal: <\/strong>Cytometry A (2009): 225<\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

How does gram stain work?<\/a>
What are sources of antibiotics?<\/a>
What are the functions of CD14?<\/a>
What does RNA polymerase do in bacteria?<\/a>
What group of bacteria normally doesn\u2019t have cell walls and how do they maintain themselves?<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

MycoLight\u2122 Green JJ98 es una tinci\u00f3n cromos\u00f3mica y nuclear verde fluorescente que es permeable a las membranas celulares tanto procari\u00f3ticas como eucari\u00f3ticas. Es un excelente reemplazo para SYTO\u00ae 9, marca registrada de Invitrogen.<\/p>\n","protected":false},"featured_media":2742,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2740"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2740\/revisions"}],"predecessor-version":[{"id":8353,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2740\/revisions\/8353"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2742"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2740"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}