{"id":2726,"date":"2023-01-03T09:54:00","date_gmt":"2023-01-03T15:54:00","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2726"},"modified":"2023-01-19T17:48:14","modified_gmt":"2023-01-19T23:48:14","slug":"calcein-am-cas-148504-34-1","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calcein-am-cas-148504-34-1\/","title":{"rendered":"Calcein, AM *CAS 148504-34-1*"},"content":{"rendered":"\n

La Calce\u00edna AM pasa f\u00e1cilmente a trav\u00e9s de la membrana celular de las c\u00e9lulas viables debido a su mayor hidrofobicidad en comparaci\u00f3n con la Calce\u00edna. El derivado de acetometoxi (AM) de la calce\u00edna (calce\u00edna AM) se usa ampliamente para marcar c\u00e9lulas vivas, ya que puede transportarse a trav\u00e9s de la membrana celular hacia las c\u00e9lulas vivas. Los grupos \u00e9ster AM enmascaran la parte de la mol\u00e9cula que quela el calcio. Al transportarse a las c\u00e9lulas vivas, las esterasas celulares cortan los grupos AM, la mol\u00e9cula se une al calcio dentro de la c\u00e9lula (lo que resulta en la adquisici\u00f3n de una fuerte fluorescencia verde) y queda atrapada en el interior. Como las c\u00e9lulas muertas carecen de esterasas, solo se marcan las c\u00e9lulas vivas. Esta caracter\u00edstica lo hace muy \u00fatil para probar la viabilidad celular y para el marcado de c\u00e9lulas a corto plazo. <\/p>\n\n\n\n

En comparaci\u00f3n con otros reactivos de marcado de c\u00e9lulas vivas (como BCECF-AM y diacetato de carboxifluoresce\u00edna), la calce\u00edna-AM es la sonda fluorescente m\u00e1s adecuada para te\u00f1ir c\u00e9lulas viables debido a su baja citotoxicidad. La calce\u00edna no afecta significativamente las funciones celulares como la proliferaci\u00f3n o la quimiotaxis de los linfocitos. Adem\u00e1s, los ensayos de viabilidad que utilizan calce\u00edna son fiables y se correlacionan bien con el ensayo est\u00e1ndar de liberaci\u00f3n de 51Cr.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22002<\/td>Calcein, AM *CAS 148504-34-1*<\/td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar (< -15 \u00b0C); Minimizar la exposici\u00f3n a la luz <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de Flujo<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n de laser<\/td>488 nm <\/td><\/tr>
Filtro de emisi\u00f3n<\/td>530\/30 nm <\/td><\/tr>
Especificaciones instrumento<\/td>FITC channel<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Microscopio de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>FITC<\/td><\/tr>
Emisi\u00f3n<\/td>FITC<\/td><\/tr>
Placa recomendada<\/td>Pared negra, fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Lector de microplacas de fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Corte<\/td>515 nm<\/td><\/tr>
Placa Recomendada<\/td>Pared negra, fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular<\/td>994.86<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\ufeff<\/p>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>81000<\/td><\/tr>
Exitaci\u00f3n (nm)<\/td>501<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>521<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de calce\u00edna, AM CAS 148504-34-1<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>100.517 \u00b5L<\/td>502.583 \u00b5L<\/td>1.005 mL<\/td>5.026 mL<\/td>10.052 mL<\/td><\/tr>
5 mM<\/strong><\/td>20.103 \u00b5L<\/td>100.517 \u00b5L<\/td>201.033 \u00b5L<\/td>1.005 mL<\/td>2.01 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.052 \u00b5L<\/td>50.258 \u00b5L<\/td>100.517 \u00b5L<\/td>502.583 \u00b5L<\/td>1.005 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1<\/strong>. Im\u00e1genes simult\u00e1neas de c\u00e9lulas HeLa vivas y apopt\u00f3ticas marcadas con calce\u00edna AM (cat. 22002) y conjugado de anexina V-iFluor\u00ae 647 (cat. 20074).<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> Im\u00e1genes de c\u00e9lulas vivas HeLa te\u00f1idas con Calcein, AM (Cat.22002).<\/p>\n\n\n\n

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Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Im\u00e1genes de c\u00e9lulas vivas HeLa te\u00f1idas con Calcein, AM (Cat.22002). Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (Blue, Cat#17535).<\/p>\n\n\n\n

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Fig. 4<\/figcaption><\/figure>\n\n\n\n

Figura 4. <\/strong>Esquema de modulaci\u00f3n de temperatura experimental. Las c\u00e9lulas se mantuvieron inicialmente a 37\u2009\u00b0C. (A) HuMEC-1 y PBL se trataron durante 60\u2009min a 37\u2009\u00b0C. La TEM se realiz\u00f3 durante 120\u2009min a 37\u2009\u00b0C, 30\u2009\u00b0C y 18\u00b0. (B) HuMEC-1 y PBL se trataron durante 60\u2009min a 37\u2009\u00b0C, 30\u2009\u00b0C y 18\u2009\u00b0C. El TEM se realiz\u00f3 durante 120\u2009min en las mismas condiciones de temperatura que se trataron antes con HuMEC-1 y PBL. (C) HuMEC-1 y PBL se trataron durante 60\u2009min a 37\u2009\u00b0C, 30\u2009\u00b0C o 18\u2009\u00b0C con recalentamiento posterior a 37\u2009\u00b0C durante TEM durante 120\u2009min. Fuente: Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B by Bogert et al., Scientific Reports, Feb. 2016.<\/em><\/p>\n\n\n\n

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BAPTA, AM *UltraPure Grade* *CAS 126150-97-8*<\/a><\/td><\/tr>
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BCECF, AM *UltraPure grade*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n

Ver todas las 47 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Effervescent Atomizer as Novel Cell Spray Technology to Decrease the Gas-to-Liquid Ratio<\/a>
Authors: <\/strong>Thiebes, Anja Lena and Klein, Sarah and Zingsheim, Jonas and M{\\”o}ller, Georg H and G{\\”u}rzing, Stefanie and Reddemann, Manuel A and Behbahani, Mehdi and Jockenhoevel, Stefan and Cornelissen, Christian G
Journal: <\/strong>Pharmaceutics (2022): 2421<\/p>\n\n\n\n

Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion<\/a>
Authors: <\/strong>Sun, Ting-Ting and Li, Xiu-Miao and Zhu, Jun-Ya and Yao, Wen and Yang, Tian-Jing and Meng, Xiang-Rui and Yao, Jin and Jiang, Qin
Journal: <\/strong>Heliyon (2022): e10994<\/p>\n\n\n\n

Sensing of Physical Factors by Cells: Electric Field, Mechanical Forces, Physical Plasma and Light\u2014Importance for Tissue Regeneration<\/a>
Authors: <\/strong>Bielfeldt, Meike and Rebl, Henrike and Peters, Kirsten and Sridharan, Kaarthik and Staehlke, Susanne and Nebe, J Barbara
Journal: <\/strong>Biomedical Materials \\& Devices (2022): 1–16<\/p>\n\n\n\n

Drug\/bioactive eluting chitosan composite foams for osteochondral tissue engineering<\/a>
Authors: <\/strong>Samie, Muhammad and Khan, Ather Farooq and Rahman, Saeed Ur and Iqbal, Haffsah and Yameen, Muhammad Arfat and Chaudhry, Aqif Anwar and Galeb, Hanaa A and Halcovitch, Nathan R and Hardy, John G
Journal: <\/strong>International Journal of Biological Macromolecules (2022)<\/p>\n\n\n\n

The effects of acute exercise and inflammation on immune function in early-stage prostate cancer<\/a>
Authors: <\/strong>Schauer, Tim and Djurhuus, Sissal Sigmundsd{\\’o}ttir and Simonsen, Casper and Brasso, Klaus and Christensen, Jesper Frank
Journal: <\/strong>Brain, behavior, \\& immunity-health (2022): 100508<\/p>\n\n\n\n

pH-responsive biopolymer-based supramolecular architectures as biodegradable carriers for 1 DOX delivery 2<\/a>
Authors: <\/strong>Nia, Marzieh Heidari and Ashkar, Said and Munguia-Lopez, Jose G and Kinsella, Joseph M and van de Ven, Theo GM
Journal: <\/strong>(2021)<\/p>\n\n\n\n

Highly Concentrated Nitrogen-Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation<\/a>
Authors: <\/strong>Munguia-Lopez, Jose G and Jiang, Tao and Ferlatte, Audrey and Fajardo-Diaz, Juan L and Munoz-Sandoval, Emilio and Tran, Simon D and Kinsella, Joseph M
Journal: <\/strong>Advanced NanoBiomed Research (2021): 2100104<\/p>\n\n\n\n

A novel flavonoid from Fissistigma cupreonitens, 5-hydroxy-7, 8-dimethoxyflavanone, competitively inhibited the efflux function of human P-glycoprotein and reversed cancer multi-drug resistance<\/a>
Authors: <\/strong>Teng, Yu-Ning and Lin, Kun-I and Lin, Yu-Chao and Thang, Tran-Dinh and Lan, Yu-Hsuan and Hung, Chin-Chuan
Journal: <\/strong>Phytomedicine (2021): 153528<\/p>\n\n\n\n

The COL-4A1 polypeptide destroy endothelial cells through the TGF-$\\beta$\/PI3K\/AKT pathway<\/a>
Authors: <\/strong>Li, Ting and Ling, Zhonghui and Xie, Kaipeng and Wang, Yixiao and Miao, Zhijing and Ji, Xiaohong and Li, Jingyun and Hou, Wenwen and Tang, Qiuqin and Yuan, Xiaojie and others,
Journal: <\/strong>Scientific Reports (2021): 1–10<\/p>\n\n\n\n

On the relevance of ligament dynamics for cell survival in air-assisted bronchoscopic spray application<\/a>
Authors: <\/strong>G{\\”u}rzing, Stefanie and Klein, Sarah and M{\\”o}ller, Georg and Thiebes, Anja Lena and Cornelissen, Christian Gabriel and Reddemann, Manuel Armin
Journal: <\/strong>Atomization and Sprays (2021)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development<\/a>
Authors: <\/strong>Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: <\/strong>Biochemical and Biophysical Research Communications (2017)<\/p>\n\n\n\n

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: <\/strong>Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: <\/strong>Biophys J. (2006)<\/p>\n\n\n\n

A vaccination and challenge model using calcein marked fish
Authors: <\/strong>Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: <\/strong>Fish Shellfish Immunol (2006): 20<\/p>\n\n\n\n

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: <\/strong>Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: <\/strong>Cytometry A (2005): 78<\/p>\n\n\n\n

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: <\/strong>Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: <\/strong>Toxicol In Vitro (2005): 505<\/p>\n\n\n\n

Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: <\/strong>Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: <\/strong>Fish Shellfish Immunol (2004): 127<\/p>\n\n\n\n

Calcein-AM is a detector of intracellular oxidative activity
Authors: <\/strong>Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: <\/strong>Histochem Cell Biol (2004): 499<\/p>\n\n\n\n

Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: <\/strong>Mueller H, Kassack MU, Wiese M.
Journal: <\/strong>J Biomol Screen (2004): 506<\/p>\n\n\n\n

In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
Authors: <\/strong>Goto T, Kajiwara H, Yoshinari M, Fukuhara E, Kobayashi S, Tanaka T.
Journal: <\/strong>Biomaterials (2003): 3885<\/p>\n\n\n\n

The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
Authors: <\/strong>Tokudome Y, Sugibayashi K.
Journal: <\/strong>Biol Pharm Bull (2003): 1508<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Calcein<\/a>
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes<\/a>
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins<\/a>
Calcein<\/a>
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

El derivado de acetometoxi (AM) de la calce\u00edna (calce\u00edna AM) se usa ampliamente para marcar c\u00e9lulas vivas, ya que puede transportarse a trav\u00e9s de la membrana celular hacia las c\u00e9lulas vivas. <\/p>\n","protected":false},"featured_media":2736,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2726"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":10,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2726\/revisions"}],"predecessor-version":[{"id":3651,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2726\/revisions\/3651"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2736"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2726"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2726"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}