{"id":2685,"date":"2023-01-02T14:54:31","date_gmt":"2023-01-02T20:54:31","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2685"},"modified":"2023-01-19T18:09:19","modified_gmt":"2023-01-20T00:09:19","slug":"hoechst-33342-20-mm-solution-in-water","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/hoechst-33342-20-mm-solution-in-water\/","title":{"rendered":"Hoechst 33342 *20 mM solution in water*"},"content":{"rendered":"\n

Las tinciones de Hoechst son una familia de tinciones fluorescentes para marcar ADN en microscop\u00eda de fluorescencia. Debido a que estas tinciones fluorescentes marcan el ADN, tambi\u00e9n se usan com\u00fanmente para visualizar n\u00facleos y mitocondrias. Dos de estas bis-benzimidas estrechamente relacionadas se usan com\u00fanmente: Hoechst 33258 y Hoechst 33342. Ambos tintes se excitan con luz ultravioleta a alrededor de 350 nm y ambos emiten luz de fluorescencia azul\/cian alrededor de un m\u00e1ximo de emisi\u00f3n a 461 nm. Las tinciones de Hoechst se pueden usar en c\u00e9lulas vivas o fijadas y, a menudo, se usan como sustituto de otra tinci\u00f3n de \u00e1cido nucleico, DAPI. La diferencia clave entre ellos es que el grupo etilo adicional de Hoechst 33342 lo hace m\u00e1s lipof\u00edlico y, por lo tanto, m\u00e1s capaz de atravesar las membranas celulares intactas. En algunas aplicaciones, Hoechst 33258 es significativamente menos permeable. Estos tintes tambi\u00e9n se pueden usar para detectar el contenido de una muestra de ADN trazando una curva est\u00e1ndar de emisi\u00f3n a contenido.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-17535<\/td>Hoechst 33342 *20 mM solution in water*<\/td>5 mL <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de fluorescencia<\/mark>
Excitaci\u00f3n : 350 nm
Emisi\u00f3n : 461 nm
Placa recomendada : Negro s\u00f3lido, fondo transparente<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>561.93<\/td><\/tr>
Disolvente<\/td>AGUA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>352<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>454<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de agua necesario para reconstituir la masa espec\u00edfica de Hoechst 33342 *soluci\u00f3n 20 mM en agua* a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>177.958 \u00b5L<\/td>889.791 \u00b5L<\/td>1.78 mL<\/td>8.898 mL<\/td>17.796 mL<\/td><\/tr>
5 mM<\/strong><\/td>35.592 \u00b5L<\/td>177.958 \u00b5L<\/td>355.916 \u00b5L<\/td>1.78 mL<\/td>3.559 mL<\/td><\/tr>
10 mM<\/strong><\/td>17.796 \u00b5L<\/td>88.979 \u00b5L<\/td>177.958 \u00b5L<\/td>889.791 \u00b5L<\/td>1.78 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Las c\u00e9lulas HeLa se incubaron en buffer HBSS 1X con suero al 5 % para inducir la inanici\u00f3n. Despu\u00e9s de la inanici\u00f3n, las c\u00e9lulas se trataron con la soluci\u00f3n de trabajo Autophagy Green\u2122 (Cat No. 23002) durante 20 minutos en una incubadora a 37 \u00b0C, CO2 al 5 % y luego se lavaron 3 veces. Los n\u00facleos se etiquetaron con Hoechst 33342 (Cat No. 17530). Los lisosomas se marcaron con LysoBrite\u2122 Orange (Cat No. 22657).<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2. <\/strong>Se sembraron c\u00e9lulas HeLa en microplacas de 96 pocillos y se incubaron a 37 \u00b0C, CO2 al 5 % durante 24 horas. A continuaci\u00f3n, las c\u00e9lulas se ti\u00f1eron con Hoechst 33342 5 o 10 \u00b5M durante 30 minutos a 37 \u00b0C, se lavaron y se tomaron im\u00e1genes en un microscopio Keyence BZ-X. Posteriormente, las c\u00e9lulas se fijaron con formaldeh\u00eddo al 4 % durante 20 minutos a temperatura ambiente, se lavaron y se tomaron im\u00e1genes.<\/p>\n\n\n\n

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Productos relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td><\/tr>
Hoechst 33258 *CAS 23491-45-4*<\/a><\/td>352<\/td>454<\/td>460001<\/sup><\/td>0.03401<\/sup><\/td><\/tr>
Hoechst 33258 *20 mM solution in water*<\/a><\/td>352<\/td>454<\/td>460001<\/sup><\/td>0.03401<\/sup><\/td><\/tr>
Hoechst 34580 *CAS 911004-45-0*<\/a><\/td>371<\/td>438<\/td>–<\/td>–<\/td><\/tr>
Hoechst 34580 *20 mM solution in water*<\/a><\/td>371<\/td>438<\/td>–<\/td>–<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Repair Kinetics of DSB-Foci Induced by Proton and $\\alpha$-Particle Microbeams of Different Energies<\/a>
Authors: <\/strong>Belchior, Ana and Canhoto, Jo{\\~a}o F and Giesen, Ulrich and Langner, Frank and Rabus, Hans and Schulte, Reinhard
Journal: <\/strong>Life (2022): 2040<\/p>\n\n\n\n

Repair kinetics of DSB-foci induced by proton and helium ion microbeams of different energies<\/a>
Authors: <\/strong>Belchior, Ana and Canhoto, Jo{\\~a}o F and Giesen, Ulrich and Langner, Frank and Rabus, Hans and Schulte, Reinhard
Journal: <\/strong>arXiv preprint arXiv:2206.08981 (2022)<\/p>\n\n\n\n

Aptamerized silica\/gold nanocapsules for stimulated release of doxorubicin through remote two-photon excitation<\/a>
Authors: <\/strong>Tew, Lih Shin and Lee, Tsung-Hsi and Lo, Leu-Wei and Khung, Yit Lung and Chen, Nai-Tzu
Journal: <\/strong>International Journal of Smart and Nano Materials (2022): 79–99<\/p>\n\n\n\n

NAMPT Inhibitor and P73 Activator Represses P53 R175H Mutated HNSCC Cell Proliferation in a Synergistic Manner<\/a>
Authors: <\/strong>Cai, Bi-He and Bai, Zhi-Yu and Lien, Ching-Feng and Yu, Si-Jie and Lu, Rui-Yu and Wu, Ming-Han and Wu, Wei-Chen and Chen, Chia-Chi and Hsu, Yi-Chiang
Journal: <\/strong>Biomolecules (2022): 438<\/p>\n\n\n\n

Studium bun{\\v{e}}{\\v{c}}n{\\’e} toxicity vybran{\\`y}ch nano{\\v{c}}{\\’a}stic v tk{\\’a}{\\v{n}}ov{\\`y}ch kultur{\\’a}ch.<\/a>
Authors: <\/strong>Filipov{\\’a}, Marcela
Journal: <\/strong>(2020)<\/p>\n\n\n\n

Thoroughly remold the localization and signaling pathway of TLR22<\/a>
Authors: <\/strong>Ji, Jianfei and Liao, Zhiwei and Rao, Youliang and Li, Wenqian and Yang, Chunrong and Yuan, Gailing and Feng, Hao and Xu, Zhen and Shao, Jianzhong and Su, Jianguo
Journal: <\/strong>Frontiers in immunology (2020): 3003<\/p>\n\n\n\n

Plant-derived alkaloid sinomenine potentiates glucocorticoid pharmacodynamics in mitogen-activated human peripheral blood mononuclear cells by regulating the translocation of glucocorticoid receptor<\/a>
Authors: <\/strong>Xu, Wencheng and Wang, Xiaoqin and Tu, Yuanchao and Masaki, Hiroshi and Tanaka, Sachiko and Onda, Kenji and Sugiyama, Kentaro and Yamada, Haruki and Hirano, Toshihiko
Journal: <\/strong>Phytotherapy research (2019): 187–196<\/p>\n\n\n\n

Tetrandrine and cepharanthine induce apoptosis through caspase cascade regulation, cell cycle arrest, MAPK activation and PI3K\/Akt\/mTOR signal modification in glucocorticoid resistant human leukemia Jurkat T cells<\/a>
Authors: <\/strong>Xu, Wencheng and Wang, Xiaoqin and Tu, Yuanchao and Masaki, Hiroshi and Tanaka, Sachiko and Onda, Kenji and Sugiyama, Kentaro and Yamada, Haruki and Hirano, Toshihiko
Journal: <\/strong>Chemico-Biological Interactions (2019): 108726<\/p>\n\n\n\n

Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages<\/a>
Authors: <\/strong>Lai, Chih-Ho and Huang, Ju-Chun and Cheng, Hsin-Hung and Wu, Meng-Chen and Huang, Mei-Zi and Hsu, Hui-Ying and Chen, Yu-An and Hsu, Chung-Yao and Pan, Yi-Jiun and Chu, Yen-Ting and others, undefined
Journal: <\/strong>Cellular microbiology (2018): e12947<\/p>\n\n\n\n

DOTA Functionalized Cross-Linked Small-Molecule Micelles for Theranostics Combining Magnetic Resonance Imaging and Chemotherapy<\/a>
Authors: <\/strong>Feng, Jianyue and Luo, Qiang and Chen, Yun and Li, Bing and Luo, Kui and Lan, Jingbo and Yu, Yunlong and Zhang, Shiyong
Journal: <\/strong>Bioconjugate chemistry (2018)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 42 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Usefulness of a triple fluorochrome combination Merocyanine 540\/Yo-Pro 1\/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: <\/strong>Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: <\/strong>Theriogenology (2006): 1122<\/p>\n\n\n\n

Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: <\/strong>Zhang X, Kiechle FL.
Journal: <\/strong>Ann Clin Lab Sci (2006): 185<\/p>\n\n\n\n

The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression
Authors: <\/strong>Li L, Yang L, Kotin RM.
Journal: <\/strong>J Gene Med (2005): 420<\/p>\n\n\n\n

Resistance mechanism development to the topoisomerase-I inhibitor Hoechst 33342 by Leishmania donovani
Authors: <\/strong>Marquis JF, Hardy I, Olivier M.
Journal: <\/strong>Parasitology (2005): 197<\/p>\n\n\n\n

Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: <\/strong>Aleman C, Namba AM, Casanovas J.
Journal: <\/strong>J Biomol Struct Dyn (2005): 29<\/p>\n\n\n\n

Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: <\/strong>Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: <\/strong>Arch Androl (2005): 371<\/p>\n\n\n\n

Evidence for a qualitative hierarchy within the Hoechst-33342 ‘side population’ (SP) of murine bone marrow cells
Authors: <\/strong>Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: <\/strong>Bone Marrow Transplant (2005): 807<\/p>\n\n\n\n

Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: <\/strong>Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: <\/strong>Cytometry A (2005): 40<\/p>\n\n\n\n

An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
Authors: <\/strong>Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: <\/strong>Hum Exp Toxicol (2005): 573<\/p>\n\n\n\n

Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line
Authors: <\/strong>Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: <\/strong>Indian J Exp Biol (2005): 313<\/p>\n\n\n\n

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Relative Brightness of Fluorescent Dyes<\/mark> (en Ingles) <\/mark><\/a><\/h6>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

Las tinciones de Hoechst son una familia de tinciones fluorescentes para marcar ADN en microscop\u00eda de fluorescencia. <\/p>\n","protected":false},"featured_media":2686,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2685"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2685\/revisions"}],"predecessor-version":[{"id":3659,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2685\/revisions\/3659"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2686"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2685"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}