{"id":2657,"date":"2023-01-02T12:56:30","date_gmt":"2023-01-02T18:56:30","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2657"},"modified":"2023-01-19T18:16:42","modified_gmt":"2023-01-20T00:16:42","slug":"dapi-46-diamidino-2-phenylindole-dihydrochloride-10-mm-solution-in-water","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/dapi-46-diamidino-2-phenylindole-dihydrochloride-10-mm-solution-in-water\/","title":{"rendered":"DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*\u00a0\u00a0\u00a0"},"content":{"rendered":"\n

DAPI es una tinci\u00f3n fluorescente que se une fuertemente al ADN. Se utiliza ampliamente en microscop\u00eda de fluorescencia. Dado que DAPI atraviesa una membrana celular intacta, se puede utilizar para te\u00f1ir c\u00e9lulas vivas adem\u00e1s de c\u00e9lulas fijadas. Para la microscop\u00eda de fluorescencia, DAPI se excita con luz ultravioleta. Cuando se une a ADN de doble cadena, su m\u00e1ximo de absorci\u00f3n es de 358 nm y su m\u00e1ximo de emisi\u00f3n es de 461 nm. Un inconveniente de DAPI es que su emisi\u00f3n es bastante amplia. DAPI tambi\u00e9n se une al ARN, aunque no es tan fuertemente fluorescente como se une al ADN. Su emisi\u00f3n cambia a alrededor de 500 nm cuando se une al ARN. La emisi\u00f3n azul de DAPI es conveniente para los ensayos de multiplexaci\u00f3n, ya que hay muy poca superposici\u00f3n de fluorescencia entre DAPI y mol\u00e9culas fluorescentes verdes como la fluoresce\u00edna y la prote\u00edna fluorescente verde (GFP), o manchas fluorescentes rojas como Texas Red. Adem\u00e1s de marcar n\u00facleos celulares, DAPI tambi\u00e9n se utiliza para la detecci\u00f3n de micoplasma o ADN de virus en cultivos celulares.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-17507<\/td>DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride]<\/td>2 ml<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar (<-15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>350.25<\/td><\/tr>
Disolvente<\/td>AGUA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>27000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>359<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>457<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora\u00a0<\/mark>\u00a0\u00a0<\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de agua necesario para reconstituir la masa espec\u00edfica de DAPI [4,6-diamidino-2-fenilindol, diclorhidrato] *soluci\u00f3n 10 mM en agua* a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>285.51 \u00b5L<\/td>1.428 mL<\/td>2.855 mL<\/td>14.276 mL<\/td>28.551 mL<\/td><\/tr>
5 mM<\/strong><\/td>57.102 \u00b5L<\/td>285.51 \u00b5L<\/td>571.021 \u00b5L<\/td>2.855 mL<\/td>5.71 mL<\/td><\/tr>
10 mM<\/strong><\/td>28.551 \u00b5L<\/td>142.755 \u00b5L<\/td>285.51 \u00b5L<\/td>1.428 mL<\/td>2.855 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

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Figura 1.<\/strong> La infecci\u00f3n por L. pneumophila aumenta la secreci\u00f3n de EV en las c\u00e9lulas THP-1. ( a ) Cantidad de veh\u00edculos el\u00e9ctricos en respuesta a la infecci\u00f3n por L. pneumophila. Las c\u00e9lulas THP-1 se trataron con IL-1\u03b2 (1\u2009ng\/mL) o se infectaron con L. pneumophila (MOI 0,25 o 0,5, respectivamente) durante 24\u2009h. La NTA se realiz\u00f3 con las distintas fracciones de part\u00edculas separadas por centrifugaci\u00f3n diferencial. ( b ) Western blot para prote\u00ednas marcadoras exos\u00f3micas. Se utilizaron lisados de c\u00e9lulas enteras o sedimentos de 100\u2009k derivados de c\u00e9lulas THP-1 no infectadas. Se cargaron cantidades iguales de prote\u00edna. (c) Microscop\u00eda electr\u00f3nica de transmisi\u00f3n con sedimento de 100\u2009k. Los EV purificados de las c\u00e9lulas THP-1 se fijaron y visualizaron despu\u00e9s de la tinci\u00f3n negativa con acetato de uranilo. La barra de escala representa 100\u2009nm. ( d ) Captaci\u00f3n de veh\u00edculos el\u00e9ctricos derivados de A549 por c\u00e9lulas THP-1. Las c\u00e9lulas A549 se ti\u00f1eron con el colorante de membrana PKH67. Los veh\u00edculos el\u00e9ctricos se recolectaron (pellet de 100\u2009k) y se incubaron con c\u00e9lulas THP-1 durante 1 o 3\u2009h, respectivamente. Los n\u00facleos se ti\u00f1eron con DAPI y se tomaron fotograf\u00edas con un aumento original de 630x. A: Los datos se muestran como media\u2009+\u2009SEM de tres experimentos independientes. **p\u2009<\u20090.01, ***p\u2009<\u20090.001, ****p\u2009<\u20090.0001. B\u2013D: resultados representativos. Fuente: La infecci\u00f3n por Legionella pneumophila activa las c\u00e9lulas transe\u00fantes de manera diferencial mediante ves\u00edculas de c\u00e9lulas bacterianas y hu\u00e9sped por Jung et al., Scientific Reports, julio de 2017.<\/p>\n\n\n\n

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Figura. 2<\/strong> Estructura qu\u00edmica de DAPI [4,6-diamidino-2-fenilindol, diclorhidrato] soluci\u00f3n 10 mM en agua<\/em><\/p>\n\n\n\n

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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 39 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

The sage genome provides insight into the evolutionary dynamics of diterpene biosynthesis gene cluster in plants<\/a>
Authors: <\/strong>Li, Chen-Yi and Yang, Lei and Liu, Yan and Xu, Zhou-Geng and Gao, Jian and Huang, Yan-Bo and Xu, Jing-Jing and Fan, Hang and Kong, Yu and Wei, Yu-Kun and others,
Journal: <\/strong>Cell Reports (2022): 111236<\/p>\n\n\n\n

Adipose-Derived Stem Cell-Incubated HA-Rich Sponge Matrix Implant Modulates Oxidative Stress to Enhance VEGF and TGF-$\\beta$ Secretions for Extracellular Matrix Reconstruction In Vivo<\/a>
Authors: <\/strong>Cheng, Yu-Shen and Yen, Hung-Hsun and Chang, Chung-Yen and Lien, Wei-Chih and Huang, Shu-Hung and Lee, Su-Shin and Wang, Lin and Wang, Hui-Min David
Journal: <\/strong>Oxidative Medicine and Cellular Longevity (2022)<\/p>\n\n\n\n

Carboxyl-Terminal Decoy Epitopes in the Capsid Protein of Porcine Circovirus Type 2 Are Immunogenicity-Enhancers That Elicit Predominantly Specific Antibodies in Non-Vaccinated Pigs<\/a>
Authors: <\/strong>Hung, Ling-Chu
Journal: <\/strong>Viruses (2022): 2373<\/p>\n\n\n\n

Modulating Microglia\/Macrophage Activation by CDNF Promotes Transplantation of Fetal Ventral Mesencephalic Graft Survival and Function in a Hemiparkinsonian Rat Model<\/a>
Authors: <\/strong>Tseng, Kuan-Yin and Wu, Jui-Sheng and Chen, Yuan-Hao and Airavaara, Mikko and Cheng, Cheng-Yi and Ma, Kuo-Hsing
Journal: <\/strong>Biomedicines (2022): 1446<\/p>\n\n\n\n

DLP 3D printed silica-doped HAp ceramic scaffolds inspired by the trabecular bone structure<\/a>
Authors: <\/strong>Zhang, Chengyu and Yuan, Yanping and Zeng, Yong and Chen, Jimin
Journal: <\/strong>Ceramics International (2022): 27765–27773<\/p>\n\n\n\n

Assessment of Cellular Alignment and Proliferation on Cellulose Nanofibril (CNF) Films<\/a>
Authors: <\/strong>Roozbahani, Sahar
Journal: <\/strong>(2022)<\/p>\n\n\n\n

The Autophagy Receptor TAX1BP1 (T6BP) improves antigen presentation by MHC-II molecules<\/a>
Authors: <\/strong>Sarango, Gabriela and Richetta, Cl{\\’e}mence and Pereira, Mathias and Kumari, Anita and Ghosh, Michael and Bertrand, Lisa and Pionneau, C{\\’e}dric and Le Gall, Morgane and Gr{\\’e}goire, Sylvie and Jeger-Madiot, Rapha{\\”e}l and others,
Journal: <\/strong>EMBO reports (2022): e55470<\/p>\n\n\n\n

Novel SPEF2 Variant in a Japanese Patient with Primary Ciliary Dyskinesia: A Case Report and Literature Review<\/a>
Authors: <\/strong>Mori, Mayako and Kido, Takashi and Sakamoto, Noriho and Ozasa, Mutsumi and Kido, Kumiko and Noguchi, Yasuko and Tokito, Takatomo and Okuno, Daisuke and Yura, Hirokazu and Hara, Atsuko and others,
Journal: <\/strong>Journal of Clinical Medicine (2022): 317<\/p>\n\n\n\n

A robust mechanism for resetting juvenility during each generation in Arabidopsis<\/a>
Authors: <\/strong>Gao, Jian and Zhang, Ke and Cheng, Ying-Juan and Yu, Sha and Shang, Guan-Dong and Wang, Fu-Xiang and Wu, Lian-Yu and Xu, Zhou-Geng and Mai, Yan-Xia and Zhao, Xin-Yan and others,
Journal: <\/strong>Nature Plants (2022): 257–268<\/p>\n\n\n\n

Dihydromyricetin suppresses cell metastasis in human osteosarcoma through SP-1-and NF-$\\kappa$B-modulated urokinase plasminogen activator inhibition<\/a>
Authors: <\/strong>Chou, Chia-Hsuan and Lu, Ko-Hsiu and Yang, Jia-Sin and Hsieh, Yi-Hsien and Lin, Chiao-Wen and Yang, Shun-Fa
Journal: <\/strong>Phytomedicine (2021): 153642<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 134 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry–new prospects for plant research
Authors: <\/strong>Suda J, Travnicek P.
Journal: <\/strong>Cytometry A (2006): 273<\/p>\n\n\n\n

Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads
Authors: <\/strong>Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM.
Journal: <\/strong>Chromosome Res (2006): 673<\/p>\n\n\n\n

Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L
Authors: <\/strong>Chen JM, Hong YH, Wang YP, Bowley S, Wan JM.
Journal: <\/strong>Yi Chuan (2006): 184<\/p>\n\n\n\n

DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I
Authors: <\/strong>Gichner T, Mukherjee A, Veleminsky J.
Journal: <\/strong>Mutat Res (2006): 17<\/p>\n\n\n\n

Investigation of chromosomes in varieties and translocation lines of pea Pisum sativum L. by FISH, Ag-NOR, and differential DAPI staining
Authors: <\/strong>Samatadze TE, Muravenko OM, Bol’sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV.
Journal: <\/strong>Genetika (2005): 1665<\/p>\n\n\n\n

DAPI fluorescence in nuclei isolated from tumors
Authors: <\/strong>Krishan A, D and ekar PD., undefined
Journal: <\/strong>J Histochem Cytochem (2005): 1033<\/p>\n\n\n\n

Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes
Authors: <\/strong>Barcellona ML, Gammon S, Hazlett T, Digman MA, Gratton E.
Journal: <\/strong>Microsc Res Tech (2004): 205<\/p>\n\n\n\n

DNA quantification in nuclei of cultivated mushroom with DAPI staining
Authors: <\/strong>Pancheva EV, Volkova VN, Kamzolkina OV.
Journal: <\/strong>Tsitologiia (2004): 381<\/p>\n\n\n\n

DAPI derivative: a fluorescent DNA dye that can be covalently attached to biomolecules
Authors: <\/strong>Li M, Wu RS, Tsai JS.
Journal: <\/strong>Bioorg Med Chem Lett (2003): 4351<\/p>\n\n\n\n

Quantification of sPLA2-induced early and late apoptosis changes in neuronal cell cultures using combined TUNEL and DAPI staining
Authors:\u00a0<\/strong>Daniel B, DeCoster MA.
Journal:\u00a0<\/strong>Brain Res Brain Res Protoc\u00a0(2004):\u00a0144<\/p>\n\n\n\n

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Guia de Selecci\u00f3n tintes iFlour\u00ae<\/a><\/strong><\/mark><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

DAPI es una tinci\u00f3n fluorescente que se une fuertemente al ADN. Se utiliza ampliamente en microscop\u00eda de fluorescencia. Adem\u00e1s de marcar n\u00facleos celulares, DAPI tambi\u00e9n se utiliza para la detecci\u00f3n de micoplasma o ADN de virus en cultivos celulares.<\/p>\n","protected":false},"featured_media":2660,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2657"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":12,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2657\/revisions"}],"predecessor-version":[{"id":3663,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2657\/revisions\/3663"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2660"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2657"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2657"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}