{"id":2630,"date":"2022-12-31T11:28:39","date_gmt":"2022-12-31T17:28:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2630"},"modified":"2023-01-19T18:18:51","modified_gmt":"2023-01-20T00:18:51","slug":"fdp-fluorescein-diphosphate-tetraammonium-salt","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/fdp-fluorescein-diphosphate-tetraammonium-salt\/","title":{"rendered":"FDP [Fluorescein diphosphate, tetraammonium salt]"},"content":{"rendered":"\n

Tras la interacci\u00f3n con las fosfatasas, el FDP incoloro y no fluorescente se hidroliza a fluoresce\u00edna altamente fluorescente, que exhibe excelentes propiedades espectrales que coinciden con la ventana de detecci\u00f3n \u00f3ptima de la mayor\u00eda de los instrumentos de fluorescencia que est\u00e1n equipados con excitaci\u00f3n por l\u00e1ser de arg\u00f3n. Alternativamente, FDP tambi\u00e9n se puede utilizar para detectar fosfatasas en un modo cromog\u00e9nico ya que el producto enzim\u00e1tico (fluoresce\u00edna) presenta un gran coeficiente de extinci\u00f3n (cerca de 100.000 cm-1mol-1). En alguna literatura, FDP se consider\u00f3 como uno de los sustratos de fosfatasa fluorog\u00e9nica m\u00e1s sensibles. FDP ha sido ampliamente utilizado en varios ensayos ELISA. Adem\u00e1s, tambi\u00e9n se utiliza para detectar tirosina fosfatasas. FDP es t\u00e9rmicamente inestable, y se deben tomar precauciones especiales para almacenar la muestra s\u00f3lida y las soluciones<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11600<\/td>FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* <\/td>5 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz. FDP es t\u00e9rmicamente inestable, y se deben tomar precauciones especiales para almacenar la muestra s\u00f3lida y las soluciones<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de microplacas de fluorescencia<\/mark>
Excitaci\u00f3n 490 nm
Emisi\u00f3n 514nm
Corte 500nm
Placa recomendada Negro s\u00f3lido<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>560.39<\/td><\/tr>
Disolvente<\/td>AGUA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Absorbancia<\/td>487<\/td><\/tr>
Factor de correcci\u00f3n (260 nm)<\/td>0.32<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.275<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>800001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>498<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>517<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.79002<\/sup>, 0.951<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> 0.1N NaOH, 2<\/sup> EtOH (Neutral)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de agua necesario para reconstituir la masa espec\u00edfica de FDP [difosfato de fluoresce\u00edna, sal de tetraamonio] *CAS 217305-49-2* a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>178.447 \u00b5L<\/td>892.236 \u00b5L<\/td>1.784 mL<\/td>8.922 mL<\/td>17.845 mL<\/td><\/tr>
5 mM<\/strong><\/td>35.689 \u00b5L<\/td>178.447 \u00b5L<\/td>356.894 \u00b5L<\/td>1.784 mL<\/td>3.569 mL<\/td><\/tr>
10 mM<\/strong><\/td>17.845 \u00b5L<\/td>89.224 \u00b5L<\/td>178.447 \u00b5L<\/td>892.236 \u00b5L<\/td>1.784 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Figura 1.<\/strong> Estructura qu\u00edmica de FDP [difosfato de fluoresce\u00edna, sal de tetraamonio] CAS 217305-49-2<\/em><\/p>\n\n\n\n

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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Allosteric inhibition of PPM1D serine\/threonine phosphatase via an altered conformational state<\/a>
Authors: <\/strong>Miller, Peter G and Sathappa, Murugappan and Moroco, Jamie A and Jiang, Wei and Qian, Yue and Iqbal, Sumaiya and Guo, Qi and Giacomelli, Andrew O and Shaw, Subrata and Vernier, Camille and others,
Journal: <\/strong>Nature Communications (2022): 1–16<\/p>\n\n\n\n

Ratiometric fluorescent sensing and imaging of intracellular pH by an AIE-active luminogen with intrinsic phosphatase-like catalytic activity<\/a>
Authors: <\/strong>Dai, Ling and Mao, Weilin and Hu, Lianzhe and Song, Jiaxing and Zhang, Yan and Huang, Ting and Wang, Min
Journal: <\/strong>Dyes and Pigments (2022): 110436<\/p>\n\n\n\n

On-Chip Enrichment System for Digital Bioassay Based on Aqueous Two-Phase System<\/a>
Authors: <\/strong>Minagawa, Yoshihiro and Nakata, Shoki and Date, Motoki and Ii, Yutaro and Noji, Hiroyuki
Journal: <\/strong>ACS nano (2022)<\/p>\n\n\n\n

Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array<\/a>
Authors: <\/strong>Zhang, Yi and Minagawa, Yoshihiro and Kizoe, Hiroto and Miyazaki, Kentaro and Iino, Ryota and Ueno, Hiroshi and Tabata, Kazuhito V and Shimane, Yasuhiro and Noji, Hiroyuki
Journal: <\/strong>Science advances (2019): eaav8185<\/p>\n\n\n\n

Fluorescence quantification of intracellular materials at the single-cell level by an integrated dual-well array microfluidic device<\/a>
Authors: <\/strong>Wang, Chenyu and Ren, Lufeng and Liu, Wenwen and Wei, Qingquan and Tan, Manqing and Yu, Yude
Journal: <\/strong>Analyst (2019)<\/p>\n\n\n\n

Multifunctional and Programmable Modulated Interface Reactions on Proteinosomes<\/a>
Authors: <\/strong>Zhou, Pei and Wu, Shuang and Liu, Xiaoman and Wu, Guangyu and Hegazy, Mohammad and Huang, Xin
Journal: <\/strong>ACS Applied Materials & Interfaces (2018)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Enzyme-nanoparticle functionalization of three-dimensional protein scaffolds
Authors: <\/strong>Hill RT, Shear JB.
Journal: <\/strong>Anal Chem (2006): 7022<\/p>\n\n\n\n

TNP-8N3-ADP photoaffinity labeling of two Na,K-ATPase sequences under separate Na+ plus K+ control
Authors: <\/strong>Ward DG, Taylor M, Lilley KS, Cavieres JD.
Journal: <\/strong>Biochemistry (2006): 3460<\/p>\n\n\n\n

Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development
Authors: <\/strong>Devader C, Webb RJ, Thomas GM, Dale L.
Journal: <\/strong>Gene (2006): 135<\/p>\n\n\n\n

Immunoassay for B. globigii spores as a model for detecting B. anthracis spores in finished water
Authors: <\/strong>Farrell S, Halsall HB, Heineman WR.
Journal: <\/strong>Analyst (2005): 489<\/p>\n\n\n\n

Controlled initiation of enzymatic reactions in micrometer-sized biomimetic compartments
Authors: <\/strong>Karlsson A, Sott K, Markstrom M, Davidson M, Konkoli Z, Orwar O.
Journal: <\/strong>J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2005): 1609<\/p>\n\n\n\n

Protein tyrosine phosphatase: enzymatic assays
Authors: <\/strong>Montalibet J, Skorey KI, Kennedy BP.
Journal: <\/strong>Methods (2005): 2<\/p>\n\n\n\n

Assaying Cdc25 phosphatase activity
Authors: <\/strong>Hassepass I, Hoffmann I.
Journal: <\/strong>Methods Mol Biol (2004): 153<\/p>\n\n\n\n

FITC binding site and p-nitrophenyl phosphatase activity of the Kdp-ATPase of Escherichia coli
Authors: <\/strong>Bramkamp M, Gassel M, Altendorf K.
Journal: <\/strong>Biochemistry (2004): 4559<\/p>\n\n\n\n

Flow injection analysis in a microfluidic format
Authors: <\/strong>Leach AM, Wheeler AR, Zare RN.
Journal: <\/strong>Anal Chem (2003): 967<\/p>\n\n\n\n

Measuring the specific activity of the CD45 protein tyrosine phosphatase
Authors: <\/strong>Rider DA, Young SP.
Journal: <\/strong>J Immunol Methods (2003): 127<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

FDP ha sido ampliamente utilizado en varios ensayos ELISA. Adem\u00e1s, tambi\u00e9n se utiliza para detectar tirosina fosfatasas. <\/p>\n","protected":false},"featured_media":2632,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2630"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2630\/revisions"}],"predecessor-version":[{"id":3665,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2630\/revisions\/3665"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2632"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2630"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}