{"id":2490,"date":"2022-12-30T16:40:47","date_gmt":"2022-12-30T22:40:47","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2490"},"modified":"2023-01-19T18:20:17","modified_gmt":"2023-01-20T00:20:17","slug":"readiuse-preactivated-apc-ifluor-700-tandem","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/readiuse-preactivated-apc-ifluor-700-tandem\/","title":{"rendered":"ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem"},"content":{"rendered":"\n

La aloficocianina (APC) es una ficobiliprote\u00edna aislada de Spirulina sp., un alga azul-verde. Al igual que otras ficobiliprote\u00ednas, APC es fluorescente, con una absorbencia extremadamente alta y una eficiencia cu\u00e1ntica alta. Es una prote\u00edna que se puede unir f\u00e1cilmente a anticuerpos y otras prote\u00ednas mediante t\u00e9cnicas convencionales de entrecruzamiento de prote\u00ednas sin alterar sus caracter\u00edsticas espectrales. Nuestro APC-iFluor\u00ae 700 Tandem es un excelente reemplazo para APC-Alexa Fluor\u00ae 700 Tandem ya que tienen espectros casi id\u00e9nticos. En algunos anticuerpos, nuestro APC-iFluor\u00ae 700 Tandem es mucho m\u00e1s brillante que APC-Alexa Fluor\u00ae 700 Tandem con un \u00edndice de tinci\u00f3n m\u00e1s alto. ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem es una prote\u00edna APC activada y se puede conjugar f\u00e1cilmente con anticuerpos con un rendimiento de conjugaci\u00f3n mucho mayor que el APC convencional.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-2570<\/td>ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem<\/td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

A<\/strong>: ReadiUse\u2122 Preactivated APC-iFluor\u2122 700 Tandem<\/td>1 vial (1 mg)<\/td><\/tr>
B<\/strong>: Buccutite\u2122 MTA<\/td>1 vial (100 \u00b5g)<\/td><\/tr>
C<\/strong>: Spin Desalting Column<\/td>No se incluye<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>700000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>651<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>710<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

imagen<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Nuestro preactivado APC-iFluor\u00ae 700 Tandem fue premodificado con nuestro Buccutite\u2122 FOL (provisto). Nuestro anticuerpo (u otras prote\u00ednas) est\u00e1  modificado con nuestro Buccutite\u2122 MTA (suministrado como muestra gratuita) para proporcionar una prote\u00edna modificada con MTA (como un anticuerpo). La prote\u00edna modificada con MTA reacciona f\u00e1cilmente con APC-iFluor\u00ae 700 Tandem modificado con FOL (incluido) dando el deseado conjugado en t\u00e1ndem del  anticuerpo APC-iFluor\u00ae 700 Tandem con un rendimiento mucho mayor que la qu\u00edmica SMCC. Adem\u00e1s, nuestro APC-iFluor\u00ae 700 Tandem preactivado reacciona con biopol\u00edmeros modificados con MTA en concentraciones mucho m\u00e1s bajas que la qu\u00edmica SMCC.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 750 Tandem<\/a><\/td>754<\/td>793<\/td>700000<\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 800 Tandem<\/a><\/td>804<\/td>660<\/td>700000<\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 700 Tandem<\/a><\/td>566<\/td>708<\/td>1960000<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: <\/strong>Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: <\/strong>J Biol Chem (2006): 8573<\/p>\n\n\n\n

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: <\/strong>Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: <\/strong>Photochem Photobiol Sci (2005): 1016<\/p>\n\n\n\n

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: <\/strong>Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: <\/strong>Biophys J (2004): 2598<\/p>\n\n\n\n

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: <\/strong>Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: <\/strong>Acta Biol Hung (2003): 113<\/p>\n\n\n\n

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: <\/strong>Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: <\/strong>Folia Microbiol (Praha) (2003): 59<\/p>\n\n\n\n

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: <\/strong>Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, T and eau de Marsac N, Cobley JG, Houmard J.
Journal: <\/strong>Mol Microbiol (2002): 749<\/p>\n\n\n\n

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: <\/strong>Ting CS, Rocap G, King J, Chisholm SW.
Journal: <\/strong>Microbiology (2001): 3171<\/p>\n\n\n\n

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: <\/strong>Triantafilou K, Triantafilou M, Wilson KM.
Journal: <\/strong>Cytometry (2000): 226<\/p>\n\n\n\n

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: <\/strong>Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: <\/strong>FEBS Lett (2000): 9<\/p>\n\n\n\n

Phycobiliprotein and fluorescence immunological assay
Authors: <\/strong>Wu P., undefined
Journal: <\/strong>Sheng Li Ke Xue Jin Zhan (2000): 82<\/p>\n\n\n\n

<\/div>\n\n\n\n

Aplication Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Buccutite\u2122 Bioconjugation Technology<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

APC es fluorescente, con una absorbencia extremadamente alta y una eficiencia cu\u00e1ntica alta.<\/p>\n","protected":false},"featured_media":2492,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2490"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2490\/revisions"}],"predecessor-version":[{"id":3667,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2490\/revisions\/3667"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2492"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2490"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2490"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}