{"id":2482,"date":"2022-12-30T16:00:28","date_gmt":"2022-12-30T22:00:28","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2482"},"modified":"2023-01-19T18:21:45","modified_gmt":"2023-01-20T00:21:45","slug":"pe-r-phycoerythrin","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/pe-r-phycoerythrin\/","title":{"rendered":"PE [R-Phycoerythrin]"},"content":{"rendered":"\n

La R-Ficoeritrina (PE) se a\u00edsla de algas rojas. Su pico de absorci\u00f3n principal est\u00e1 a 565 nm con picos secundarios a 496 y 545 nm. La prominencia relativa de los picos secundarios var\u00eda significativamente entre los R-PE de diferentes especies. PE tiene tres tipos de subunidades: alfa (20.000 daltons), beta (20.000 daltons) y gamma (30.000 daltons). Se ha encontrado que el peso molecular del PE intacto es de aproximadamente 240.000 daltons. La subunidad alfa de PE contiene solo el crom\u00f3foro ficoeritrobilina (PEB), mientras que las subunidades beta y gamma contienen tanto PEB como ficourobilina (PUB). La variabilidad en los espectros de absorci\u00f3n de PE de varias especies refleja diferencias en la relaci\u00f3n PEB\/PUB de las subunidades. La PE y la B-PE estrechamente relacionada son las ficobiliprote\u00ednas m\u00e1s intensamente fluorescentes, con eficiencias cu\u00e1nticas probablemente superiores al 90 %, y su fluorescencia naranja es f\u00e1cilmente visible a simple vista en cualquier soluci\u00f3n moderadamente concentrada.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-2558<\/td>PE [R-Phycoerythrin] *CAS 11016-17-4*<\/td>1 mg<\/td><\/tr>
AAT-2556<\/td>PE [R-Phycoerythrin] *CAS 11016-17-4*<\/td>10 mg<\/td><\/tr>
AAT-2557<\/td>PE [R-Phycoerythrin] *CAS 11016-17-4*<\/td>100 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigeraci\u00f3n (2-8 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>-240000<\/td><\/tr>
Disolvente<\/td>AGUA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1960000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>566<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>574<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.82<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Arriba) El patr\u00f3n espectral se gener\u00f3 utilizando un cit\u00f3metro espectral de 4 l\u00e1seres. Se utilizaron l\u00e1seres desplazados espacialmente (355 nm, 405 nm, 488 nm y 640 nm) para crear cuatro perfiles de emisi\u00f3n distintos y luego, cuando se combinaron, produjeron la firma espectral general. Abajo) An\u00e1lisis de citometr\u00eda de flujo de PBMC te\u00f1idas con conjugado PE anti-CD4 humano *SK3*. La se\u00f1al de fluorescencia se control\u00f3 usando un cit\u00f3metro de flujo Aurora en el canal B6-A espec\u00edfico de PE.<\/p>\n\n\n\n

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Fig 2.<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> An\u00e1lisis de citometr\u00eda de flujo de sangre completa te\u00f1ida con conjugado de PE anti-CD4 humano *SK3*. La se\u00f1al de fluorescencia se control\u00f3 utilizando un cit\u00f3metro de flujo Aurora en el canal B4-A espec\u00edfico de PE.<\/p>\n\n\n\n

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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

CD169+ subcapsular sinus macrophage-derived microvesicles are associated with light zone follicular dendritic cells<\/a>
Authors: <\/strong>Chen, Xin and Zheng, Yuhan and Liu, Siming and Yu, Wenjing and Liu, Zhiduo
Journal: <\/strong>European Journal of Immunology (2022): 1581–1594<\/p>\n\n\n\n

Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice<\/a>
Authors: <\/strong>Tan, Yandi and Yang, Shiqi and Ma, Yao and Li, Jinlin and Xie, Qian and Liu, Chaoqi and Zhao, Yun
Journal: <\/strong>International Journal of Nanomedicine (2021): 3241<\/p>\n\n\n\n

Morphological Changes Induced By Extremely Low-Frequency Electric Fields<\/a>
Authors: <\/strong>Imani, Mahdi and Kazemi, Sepide and Saviz, Mehrdad and Farahm, undefined and , Leila and Sadeghi, Behnam and Faraji-dana, Reza
Journal: <\/strong>Bioelectromagnetics (2019)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: <\/strong>Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: <\/strong>J Biol Chem (2006): 8573<\/p>\n\n\n\n

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: <\/strong>Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: <\/strong>Photochem Photobiol Sci (2005): 1016<\/p>\n\n\n\n

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: <\/strong>Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: <\/strong>Biophys J (2004): 2598<\/p>\n\n\n\n

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: <\/strong>Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: <\/strong>Acta Biol Hung (2003): 113<\/p>\n\n\n\n

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: <\/strong>Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: <\/strong>Folia Microbiol (Praha) (2003): 59<\/p>\n\n\n\n

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: <\/strong>Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, T and eau de Marsac N, Cobley JG, Houmard J.
Journal: <\/strong>Mol Microbiol (2002): 749<\/p>\n\n\n\n

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: <\/strong>Ting CS, Rocap G, King J, Chisholm SW.
Journal: <\/strong>Microbiology (2001): 3171<\/p>\n\n\n\n

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: <\/strong>Triantafilou K, Triantafilou M, Wilson KM.
Journal: <\/strong>Cytometry (2000): 226<\/p>\n\n\n\n

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: <\/strong>Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: <\/strong>FEBS Lett (2000): 9<\/p>\n\n\n\n

Phycobiliprotein and fluorescence immunological assay
Authors: <\/strong>Wu P., undefined
Journal: <\/strong>Sheng Li Ke Xue Jin Zhan (2000): 82<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Phycobiliproteins and Their Fluorescent Labeling Applications<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

La R-Ficoeritrina (PE) se a\u00edsla de algas rojas. Su pico de absorci\u00f3n principal est\u00e1 a 565 nm con picos secundarios a 496 y 545 nm. <\/p>\n","protected":false},"featured_media":2488,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2482"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2482\/revisions"}],"predecessor-version":[{"id":3669,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2482\/revisions\/3669"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2488"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2482"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}