{"id":2468,"date":"2022-12-30T14:29:13","date_gmt":"2022-12-30T20:29:13","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=2468"},"modified":"2023-01-19T18:27:23","modified_gmt":"2023-01-20T00:27:23","slug":"percp-peridinin-chlorophyll-protein-complex","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/percp-peridinin-chlorophyll-protein-complex\/","title":{"rendered":"PerCP [Peridinin-Chlorophyll-Protein Complex]\u00a0\u00a0\u00a0"},"content":{"rendered":"\n<p>PerCP (complejo peridinina-clorofila-prote\u00edna) se a\u00edsla de Dinophyceae sp. Tiene un coeficiente de extinci\u00f3n extremadamente alto, una alta eficiencia cu\u00e1ntica y un gran cambio de Stokes. Se excita con el l\u00e1ser de arg\u00f3n a 488 nm con un pico m\u00e1ximo de emisi\u00f3n a 677 nm. La prote\u00edna PerCP se usa com\u00fanmente para el inmunomarcaje fluorescente, particularmente en aplicaciones que involucran la separaci\u00f3n de c\u00e9lulas activadas por fluorescencia (FACS). Sus conjugados en t\u00e1ndem de cianina (como PerCP-Cy5.5 desarrollado por BD) se pueden excitar con un l\u00e1ser est\u00e1ndar de 488 nm y emite en el rojo lejano a una longitud de onda m\u00e1s larga para el an\u00e1lisis de c\u00e9lulas por citometr\u00eda de flujo multicolor. Estas m\u00faltiples longitudes de onda de emisi\u00f3n hacen que los conjugados PerCP-cianina sean fluorocromos potencialmente \u00fatiles para el an\u00e1lisis multicolor con FITC, PE y otros fluorocromos. La estructura en t\u00e1ndem de PerCP puede hacer que sea m\u00e1s fotoestable que solo PerCP que generalmente se fotoblanquea r\u00e1pidamente con l\u00e1seres de gas enfriados con agua m\u00e1s potentes. <\/p>\n\n\n\n<p>AAT Bioquest ofrece tintes de marcaje de prote\u00ednas iFluor que generalmente son m\u00e1s brillantes y m\u00e1s fotoestables que los tintes de cianina correspondientes de longitudes de onda similares. Los tintes de etiquetado de prote\u00ednas iFluor son alternativas superiores a los tintes de cianina.<\/p>\n\n\n\n<div style=\"height:29px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>AAT-2559<\/td><td>PerCP [Peridinin-Chlorophyll-Protein Complex]<\/td><td>1 mg<\/td><\/tr><tr><td>AAT-2540<\/td><td>PerCP [Peridinin-Chlorophyll-Protein Complex]<\/td><td>10 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:44px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"><a href=\"https:\/\/docs.aatbio.com\/products\/safety-data-sheet-sds\/safety-data-sheet-for-percp-peridinin-chlorophyll-protein-complex-catalog-2559.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">SDS<\/a><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"><a href=\"https:\/\/docs.aatbio.com\/products\/protocol\/B1200d2.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Protocol<\/a><\/p>\n\n\n\n<p>Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigerar (2-8 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n<div style=\"height:64px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Propiedades fisicas<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Peso Molecular <\/td><td>-35000<\/td><\/tr><tr><td>Disolvente<\/td><td>AGUA<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:54px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Espectro<\/mark><\/p>\n\n\n\n<p><em>Abrir en&nbsp;<a href=\"https:\/\/www.aatbio.com\/fluorescence-excitation-emission-spectrum-graph-viewer\/percp_peridinin_chlorophyll\" target=\"_blank\" rel=\"noopener\" title=\"\">Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28.png\" alt=\"\" class=\"wp-image-2469\" width=\"857\" height=\"487\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28.png 1010w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28-300x170.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28-768x436.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28-600x341.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/image-28-280x160.png 280w\" sizes=\"(max-width: 857px) 100vw, 857px\" \/><\/figure>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Propiedades espectrales<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Coeficiente de extinci\u00f3n (cm&nbsp;<sup>-1<\/sup>&nbsp;M&nbsp;<sup>-1<\/sup>)<\/td><td>350000<\/td><\/tr><tr><td>Excitaci\u00f3n (nm)<\/td><td>477<\/td><\/tr><tr><td>Emisi\u00f3n (nm)<\/td><td>678<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:64px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Imagen<\/mark><\/p>\n\n\n\n<div style=\"height:16px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540.jpg\" alt=\"\" class=\"wp-image-2470\" width=\"621\" height=\"483\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540.jpg 1000w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540-300x234.jpg 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540-768x598.jpg 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540-600x467.jpg 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2022\/12\/2540-205x160.jpg 205w\" sizes=\"(max-width: 621px) 100vw, 621px\" \/><figcaption>Fig. 1<\/figcaption><\/figure>\n\n\n\n<p style=\"font-size:16px\"><strong>Figura 1.<\/strong> La clorofila de peridinina (PerCP) es un pigmento apocarotenoide que algunos organismos utilizan en la fotos\u00edntesis. Muchos dinoflagelados fotosint\u00e9ticos utilizan peridinina, que absorbe la luz azul verdosa en el rango de 470 a 550 nm, fuera del rango accesible a las mol\u00e9culas de clorofila. El complejo peridinina-clorofila-prote\u00edna es un complejo molecular especializado que consta de una mol\u00e9cula de prote\u00edna en forma de bote con una gran cavidad central que contiene mol\u00e9culas de peridinina, clorofila y l\u00edpidos, generalmente en una proporci\u00f3n de 4:1 de peridinina &nbsp;clorofila. PerCP se usa com\u00fanmente en inmunoensayos como la separaci\u00f3n de c\u00e9lulas activadas por fluorescencia (FACS) y la citometr\u00eda de flujo. El fluor\u00f3foro se une covalentemente a prote\u00ednas o anticuerpos para su uso en aplicaciones de investigaci\u00f3n.<\/p>\n\n\n\n<div style=\"height:56px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Bibliograf\u00eda<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\">Optimising biomass and peridinin production in the immobilised cultivation of the dinoflagellate Symbiodinium voratum<br><strong>Authors:&nbsp;<\/strong>Langenbach, Dorothee<br><strong>Journal:&nbsp;<\/strong>(2016)<\/p>\n\n\n\n<div style=\"height:53px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Referencias<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><em>Ver todas las 46 referencias:\u00a0<\/em><a href=\"https:\/\/www.aatbio.com\/resources\/citation-explorer?catalog=2559\" target=\"_blank\" rel=\"noopener\" title=\"\">Citation Explorer<\/a><\/p>\n\n\n\n<p style=\"font-size:14px\">Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120<br><strong>Authors:&nbsp;<\/strong>Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.<br><strong>Journal:&nbsp;<\/strong>J Biol Chem&nbsp;(2006):&nbsp;8573<\/p>\n\n\n\n<p style=\"font-size:14px\">Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy<br><strong>Authors:&nbsp;<\/strong>Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.<br><strong>Journal:&nbsp;<\/strong>Photochem Photobiol Sci&nbsp;(2005):&nbsp;1016<\/p>\n\n\n\n<p style=\"font-size:14px\">Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin<br><strong>Authors:&nbsp;<\/strong>Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.<br><strong>Journal:&nbsp;<\/strong>Biophys J&nbsp;(2004):&nbsp;2598<\/p>\n\n\n\n<p style=\"font-size:14px\">Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp<br><strong>Authors:&nbsp;<\/strong>Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.<br><strong>Journal:&nbsp;<\/strong>Acta Biol Hung&nbsp;(2003):&nbsp;113<\/p>\n\n\n\n<p style=\"font-size:14px\">Evaluation of Tolypothrix germplasm for phycobiliprotein content<br><strong>Authors:&nbsp;<\/strong>Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.<br><strong>Journal:&nbsp;<\/strong>Folia Microbiol (Praha)&nbsp;(2003):&nbsp;59<\/p>\n\n\n\n<p style=\"font-size:14px\">Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG<br><strong>Authors:&nbsp;<\/strong>Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, T and eau de Marsac N, Cobley JG, Houmard J.<br><strong>Journal:&nbsp;<\/strong>Mol Microbiol&nbsp;(2002):&nbsp;749<\/p>\n\n\n\n<p style=\"font-size:14px\">Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity<br><strong>Authors:&nbsp;<\/strong>Ting CS, Rocap G, King J, Chisholm SW.<br><strong>Journal:&nbsp;<\/strong>Microbiology&nbsp;(2001):&nbsp;3171<\/p>\n\n\n\n<p style=\"font-size:14px\">Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging<br><strong>Authors:&nbsp;<\/strong>Triantafilou K, Triantafilou M, Wilson KM.<br><strong>Journal:&nbsp;<\/strong>Cytometry&nbsp;(2000):&nbsp;226<\/p>\n\n\n\n<p style=\"font-size:14px\">Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon<br><strong>Authors:&nbsp;<\/strong>Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.<br><strong>Journal:&nbsp;<\/strong>FEBS Lett&nbsp;(2000):&nbsp;9<\/p>\n\n\n\n<p style=\"font-size:14px\">Phycobiliprotein and fluorescence immunological assay<br><strong>Authors:&nbsp;<\/strong>Wu P., undefined<br><strong>Journal:&nbsp;<\/strong>Sheng Li Ke Xue Jin Zhan&nbsp;(2000):&nbsp;82<\/p>\n\n\n\n<div style=\"height:74px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>El PerCP se a\u00edsla de Dinophyceae sp. Tiene un coeficiente de extinci\u00f3n extremadamente alto, una alta eficiencia cu\u00e1ntica y un gran cambio de Stokes. <\/p>\n","protected":false},"featured_media":2470,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2468"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2468\/revisions"}],"predecessor-version":[{"id":3673,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2468\/revisions\/3673"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2470"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2468"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}