{"id":2438,"date":"2022-12-29T15:05:35","date_gmt":"2022-12-29T21:05:35","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2438"},"modified":"2023-01-19T18:30:42","modified_gmt":"2023-01-20T00:30:42","slug":"ifluor-860-maleimide","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-860-maleimide\/","title":{"rendered":"iFluor\u00ae 860 maleimide"},"content":{"rendered":"\n

Las im\u00e1genes de fluorescencia in vivo<\/em> utilizan una c\u00e1mara sensible para detectar la emisi\u00f3n de fluorescencia de los fluor\u00f3foros en peque\u00f1os animales vivos de cuerpo entero. Para superar la atenuaci\u00f3n de fotones en el tejido vivo, generalmente se prefieren los fluor\u00f3foros con emisi\u00f3n prolongada en la regi\u00f3n infrarroja (IR). Los avances recientes en las estrategias de obtenci\u00f3n de im\u00e1genes y las t\u00e9cnicas de reportero para la obtenci\u00f3n de im\u00e1genes por fluorescencia in vivo incluyen enfoques novedosos para mejorar la especificidad y la afinidad de las sondas y para modular y amplificar la se\u00f1al en los sitios objetivo para aumentar la sensibilidad. Otros desarrollos emergentes tienen como objetivo lograr im\u00e1genes de fluorescencia in vivo de alta resoluci\u00f3n, multimodalidad y basadas en la vida \u00fatil. <\/p>\n\n\n\n

Nuestro iFluor\u00ae 860 est\u00e1 dise\u00f1ado para marcar prote\u00ednas y otras biomol\u00e9culas con fluorescencia infrarroja. Los conjugados preparados con iFluor\u00ae 860 tienen excitaci\u00f3n y emisi\u00f3n en el rango IR. La emisi\u00f3n del colorante iFluor\u00ae 860 est\u00e1 bien separada de los fluor\u00f3foros de rojo lejano com\u00fanmente utilizados, como Cy5, Cy7 o la aloficocianina (APC), lo que facilita el an\u00e1lisis multicolor. Este fluor\u00f3foro tambi\u00e9n es \u00fatil para aplicaciones de im\u00e1genes in vivo de animales peque\u00f1os u otras aplicaciones de im\u00e1genes que requieren detecci\u00f3n IR. La maleimida iFluor\u00ae 860 es reactiva con tiol y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen tiol.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1408<\/td>iFluor\u00ae 860 maleimide<\/td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1647.66<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Ad<\/a>vanced Spectrum Viewe<\/a>r<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.1<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.14<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2500001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>853<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>878<\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de iFluor\u00ae 860 maleimida a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n


<\/strong><\/td>		0.1mg<\/strong><\/td>0.5mg<\/strong><\/td>1mg<\/strong><\/td>5mg<\/strong><\/td>10mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>60.692 \u00b5L<\/td>303.461 \u00b5L<\/td>606.921 \u00b5L<\/td>3.035 mL<\/td>6.069 mL<\/td><\/tr>
5 mM<\/strong><\/td>12.138 \u00b5L<\/td>60.692 \u00b5L<\/td>121.384 \u00b5L<\/td>606.921 \u00b5L<\/td>1.214 mL<\/td><\/tr>
10 mM<\/strong><\/td>6.069 \u00b5L<\/td>30.346 \u00b5L<\/td>60.692 \u00b5L<\/td>303.461 \u00b5L<\/td>606.921 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1. <\/strong>Las maleimidas de colorantes fluorescentes (p. ej., iFluor 860 maleimida) son la herramienta m\u00e1s popular para conjugar colorantes con un p\u00e9ptido, prote\u00edna, anticuerpo, oligonucle\u00f3tido modificado con tiol o \u00e1cido nucleico a trav\u00e9s de su grupo SH. Las maleimidas reaccionan r\u00e1pidamente con el grupo tiol de las prote\u00ednas, los oligonucle\u00f3tidos modificados con tiol y otras mol\u00e9culas que contienen tiol en condiciones neutras. Los conjugados de tinte resultantes son bastante estables.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td>690<\/td>713<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.09<\/td>0.04<\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td>757<\/td>779<\/td>2750001<\/sup><\/td>0.121<\/sup><\/td>0.044<\/td>0.039<\/td><\/tr>
iFluor\u00ae 790 maleimide<\/a><\/td>787<\/td>812<\/td>2500001<\/sup><\/td>0.131<\/sup><\/td>0.1<\/td>0.09<\/td><\/tr>
iFluor\u00ae 800 maleimide<\/a><\/td>801<\/td>820<\/td>2500001<\/sup><\/td>0.111<\/sup><\/td>0.03<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Pancreatic Cancer Cells Undergo Immunogenic Cell Death upon Exposure to Gas Plasma-Oxidized Ringer\u2019s Lactate<\/a>
Authors: <\/strong>Miebach, Lea and Mohamed, Hager and Wende, Kristian and Miller, Vandana and Bekeschus, Sander
Journal: <\/strong>Cancers (2023): 319<\/p>\n\n\n\n

Identification of distinct functional thymic programming of fetal and pediatric human $\\gamma$$\\delta$ thymocytes via single-cell analysis<\/a>
Authors: <\/strong>Sanchez Sanchez, Guillem and Papadopoulou, Maria and Azouz, Abdulkader and Tafesse, Yohannes and Mishra, Archita and Chan, Jerry KY and Fan, Yiping and Verdebout, Isoline and Porco, Silvana and Libert, Fr{\\’e}d{\\’e}rick and others,
Journal: <\/strong>Nature communications (2022): 1–19<\/p>\n\n\n\n

Conductive Gas Plasma Treatment Augments Tumor Toxicity of Ringer\u2019s Lactate Solutions in a Model of Peritoneal Carcinomatosis<\/a>
Authors: <\/strong>Miebach, Lea and Freund, Eric and Cecchini, Alessandra Louren{\\c{c}}o and Bekeschus, Sander
Journal: <\/strong>Antioxidants (2022): 1439<\/p>\n\n\n\n

A versatile platform for generating engineered extracellular vesicles with defined therapeutic properties<\/a>
Authors: <\/strong>Dooley, Kevin and McConnell, Russell E and Xu, Ke and Lewis, Nuruddeen D and Haupt, Sonya and Youniss, Madeleine R and Martin, Shelly and Sia, Chang Ling and McCoy, Christine and Moniz, Raymond J and others,
Journal: <\/strong>Molecular Therapy (2021): 1729–1743<\/p>\n\n\n\n

Soluble ST2 links inflammation to outcome after subarachnoid hemorrhage<\/a>
Authors: <\/strong>Bevers, Matthew B and Wolcott, Zoe and Bache, S\u00f8ren and Hansen, Christina and Sastre, Cristina and Mylvaganam, Ravi and Koch, Matthew J and Patel, Aman B and M\u00f8ller, Kirsten and Kimberly, W Taylor
Journal: <\/strong>Annals of neurology (2019)<\/p>\n\n\n\n

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution<\/a>
Authors: <\/strong>Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: <\/strong>Nanomedicine (2017)<\/p>\n\n\n\n

18–Color Human Blood Phenotyping Made Easy with Flow Cytometry
Authors: <\/strong>McCracken, James and Lawson, Jonel<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 18 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
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Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
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In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
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A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
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In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
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In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
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In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
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In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
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In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in an orthotopic glioblastoma model
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<\/div>\n\n\n\n

Guia de Selecci\u00f3n tintes iFlour\u00ae<\/strong><\/mark><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

La maleimida iFluor\u00ae 860 es reactiva con tiol y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen tiol.<\/p>\n","protected":false},"featured_media":2441,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2438"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2438\/revisions"}],"predecessor-version":[{"id":3676,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2438\/revisions\/3676"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2441"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2438"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2438"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}