{"id":2432,"date":"2022-12-29T14:19:29","date_gmt":"2022-12-29T20:19:29","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2432"},"modified":"2023-01-19T18:32:13","modified_gmt":"2023-01-20T00:32:13","slug":"ifluor-840-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-840-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 840 succinimidyl ester"},"content":{"rendered":"\n

Las im\u00e1genes de fluorescencia in vivo<\/em> utilizan una c\u00e1mara sensible para detectar la emisi\u00f3n de fluorescencia de los fluor\u00f3foros en peque\u00f1os animales vivos de cuerpo entero. Para superar la atenuaci\u00f3n de fotones en el tejido vivo, generalmente se prefieren los fluor\u00f3foros con emisi\u00f3n prolongada en la regi\u00f3n infrarroja (IR). Los avances recientes en las estrategias de obtenci\u00f3n de im\u00e1genes y las t\u00e9cnicas de reportero para la obtenci\u00f3n de im\u00e1genes por fluorescencia in vivo incluyen enfoques novedosos para mejorar la especificidad y la afinidad de las sondas y para modular y amplificar la se\u00f1al en los sitios objetivo para mejorar la sensibilidad. Otros desarrollos emergentes tienen como objetivo lograr im\u00e1genes de fluorescencia in vivo de alta resoluci\u00f3n, multimodalidad y basadas en la vida \u00fatil. <\/p>\n\n\n\n

Nuestro iFluor\u00ae 840 est\u00e1 dise\u00f1ado para marcar prote\u00ednas y otras biomol\u00e9culas con fluorescencia infrarroja. Los conjugados preparados con iFluor\u00ae 840 tienen excitaci\u00f3n y emisi\u00f3n en el rango IR. La emisi\u00f3n de colorante iFluor\u00ae 840 est\u00e1 bien separada de los fluor\u00f3foros de rojo lejano com\u00fanmente utilizados, como Cy5, Cy7 o la aloficocianina (APC), lo que facilita el an\u00e1lisis multicolor. Este fluor\u00f3foro tambi\u00e9n es \u00fatil para aplicaciones de im\u00e1genes in vivo de animales peque\u00f1os u otras aplicaciones de im\u00e1genes que requieren detecci\u00f3n IR. El succinimidil \u00e9ster iFluor\u00ae 840 es reactivo con amina y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen amina, particularmente anticuerpos.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1403<\/td>iFluor\u00ae 840 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71403<\/td>iFluor\u00ae 840 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71528<\/td>iFluor\u00ae 840 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71578<\/td>iFluor\u00ae 840 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1422<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.02<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.09<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2000001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>836<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>879<\/td><\/tr><\/tbody><\/table>
1<\/sup> PBS<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 840 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n



<\/strong><\/td>		0.1mg<\/strong><\/td>0.5mg<\/strong><\/td>1mg<\/strong><\/td>5mg<\/strong><\/td> 10mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>70.323 \u00b5L<\/td>351.617 \u00b5L<\/td>703.235 \u00b5L<\/td>3.516 mL<\/td>7.032 mL<\/td><\/tr>
5 mM<\/strong><\/td>14.065 \u00b5L<\/td>70.323 \u00b5L<\/td>140.647 \u00b5L<\/td>703.235 \u00b5L<\/td>1.406 mL<\/td><\/tr>
10 mM<\/strong><\/td>7.032 \u00b5L<\/td>35.162 \u00b5L<\/td>70.323 \u00b5L<\/td>351.617 \u00b5L<\/td>703.235 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Los \u00e9steres NHS con colorantes fluorescentes (o \u00e9steres de succinimidil) son la herramienta m\u00e1s popular para conjugar colorantes con un p\u00e9ptido, prote\u00edna, anticuerpo, oligonucle\u00f3tido modificado con amino o \u00e1cido nucleico. Los \u00e9steres  NHS reaccionan r\u00e1pidamente con las aminas primarias (R-NH2) de prote\u00ednas, oligonucle\u00f3tidos modificados con amina y otras mol\u00e9culas que contienen amina. El resultado de los tintes conjugados resultantes son bastante estables.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding
Authors: <\/strong>Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: <\/strong>Front Neuroanat (2018): 88<\/p>\n\n\n\n

Bioactive magnetic near Infra-Red fluorescent core-shell iron oxide\/human serum albumin nanoparticles for controlled release of growth factors for augmentation of human mesenchymal stem cell growth and differentiation
Authors: <\/strong>Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: <\/strong>J Nanobiotechnology (2015): 34<\/p>\n\n\n\n

Novel near infra-red fluorescent pH sensors based on 1-aminoperylene bisimides covalently grafted onto poly(acryloylmorpholine)
Authors: <\/strong>Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: <\/strong>Chem Commun (Camb) (2013): 2139-41<\/p>\n\n\n\n

In vitro and ex vivo evaluation of smart infra-red fluorescent caspase-3 probes for molecular imaging of cardiovascular apoptosis
Authors: <\/strong>Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: <\/strong>Int J Mol Imaging (2011): 413290<\/p>\n\n\n\n

Simple sensitive and simultaneous high-performance liquid chromatography method of glucoconjugated and non-glucoconjugated porphyrins and chlorins using near infra-red fluorescence detection
Authors: <\/strong>Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: <\/strong>J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72<\/p>\n\n\n\n

An infra-red light-transmitting aperture controller for use in single-cell fluorescence photometry
Authors: <\/strong>Mahaut-Smith, M. P.
Journal: <\/strong>J Microsc (1998): 60-6<\/p>\n\n\n\n

Photosynthetic induction in C4 leaves : An investigation using infra-red gas analysis and chlorophyll a fluorescence
Authors: <\/strong>Furbank, R. T., Walker, D. A.
Journal: <\/strong>Planta (1985): 75-83<\/p>\n\n\n\n

Fluorescent infra-red angiography of the fundus oculi using indocyanine green dye
Authors: <\/strong>Chopdar, A., Turk, A. M., Hill, D. W.
Journal: <\/strong>Trans Ophthalmol Soc U K (1978): 142-6<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution<\/a>
Authors: <\/strong>Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: <\/strong>Nanomedicine (2017)<\/p>\n\n\n\n

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: <\/strong>Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: <\/strong>Cancer Res (2007): 2791<\/p>\n\n\n\n

Fluorescence imaging in vivo: recent advances
Authors: <\/strong>Rao J, Dragulescu-Andrasi A, Yao H.
Journal: <\/strong>Curr Opin Biotechnol (2007): 17<\/p>\n\n\n\n

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: <\/strong>Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: <\/strong>Photochem Photobiol (2007): 1157<\/p>\n\n\n\n

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: <\/strong>Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: <\/strong>J Immunol Methods (2007): 65<\/p>\n\n\n\n

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: <\/strong>Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: <\/strong>Nat Protoc (2007): 2166<\/p>\n\n\n\n

In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
Authors: <\/strong>Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: <\/strong>Am J Respir Crit Care Med (2007): 22<\/p>\n\n\n\n

In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: <\/strong>Szabo A, Vollmar B, Boros M, Menger MD.
Journal: <\/strong>J Surg Res. (2007)<\/p>\n\n\n\n

In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
Authors: <\/strong>Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: <\/strong>Neoplasia (2006): 607<\/p>\n\n\n\n

In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
Authors: <\/strong>Al-Gubory KH, Houdebine LM.
Journal: <\/strong>Eur J Cell Biol (2006): 837<\/p>\n\n\n\n

<\/div>\n\n\n\n
Guia de Selecci\u00f3n tintes iFlour\u00ae<\/mark><\/h6>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

El succinimidil \u00e9ster iFluor\u00ae 840 es reactivo con amina y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen amina, particularmente anticuerpos.<\/p>\n","protected":false},"featured_media":2435,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2432"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2432\/revisions"}],"predecessor-version":[{"id":3678,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2432\/revisions\/3678"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2435"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2432"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2432"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}