{"id":2420,"date":"2022-12-29T13:43:05","date_gmt":"2022-12-29T19:43:05","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2420"},"modified":"2023-01-20T11:35:46","modified_gmt":"2023-01-20T17:35:46","slug":"ifluor-700-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-700-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 700 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, particularmente anticuerpos. Estos tintes son brillantes, fotoestables y tienen una m\u00ednima  extinci\u00f3n del flourocromo sobre las prote\u00ednas. Pueden excitarse bien con los principales l\u00e1seres de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). Los colorantes iFluor\u00ae 700 tienen un m\u00e1ximo de excitaci\u00f3n y emisi\u00f3n de fluorescencia de ~690 nm y ~713 nm respectivamente. Estas caracter\u00edsticas espectrales los convierten en una excelente alternativa al colorante de etiquetado Alexa Fluor\u00ae 700 (Alexa Fluor\u00ae es la marca comercial de Invitrogen). iFluor\u00ae 700 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1036<\/td>iFluor\u00ae 700 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71036<\/td>iFluor\u00ae 700 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71514<\/td>iFluor\u00ae 700 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71564<\/td>iFluor\u00ae 700 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de microplacas de fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>691 nm<\/td><\/tr>
Emisi\u00f3n<\/td>711 nm<\/td><\/tr>
Corte<\/td>700 nm<\/td><\/tr>
Placa recomendada<\/td>Negra Solida <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>977.16<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.09<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.04<\/td><\/tr>
Factor de correcci\u00f3n (565 nm)<\/td>0.0766<\/td><\/tr>
Factor de correcci\u00f3n (656 nm)<\/td>0.4004<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2200001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>690<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>713<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.231<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 700 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>102.337 \u00b5L<\/td>511.687 \u00b5L<\/td>1.023 mL<\/td>5.117 mL<\/td>10.234 mL<\/td><\/tr>
5 mM<\/strong><\/td>20.467 \u00b5L<\/td>102.337 \u00b5L<\/td>204.675 \u00b5L<\/td>1.023 mL<\/td>2.047 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.234 \u00b5L<\/td>51.169 \u00b5L<\/td>102.337 \u00b5L<\/td>511.687 \u00b5L<\/td>1.023 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

\"\"
Fig 1.<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Las c\u00e9lulas HeLa se incubaron con (Tubulina+) o sin (Tubulina-) antitubulina de rat\u00f3n seguido de iFluor\u00ae 700 conjugado de cabra anti-IgG de rat\u00f3n (izquierda) o Alexa Fluor\u00ae 700 conjugado de cabra anti-IgG de rat\u00f3n (derecha). respectivamente. Los conjugados IgG anti-rat\u00f3n de cabra Alexa Fluor\u00ae 700 dieron una tinci\u00f3n nuclear no espec\u00edfica.<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> An\u00e1lisis de citometr\u00eda de flujo de PBMC te\u00f1idas con iFluor\u00ae 700 conjugado anti-CD3 humano *SK7*. La se\u00f1al de fluorescencia se control\u00f3 utilizando un cit\u00f3metro de flujo Aurora en el canal R4-A espec\u00edfico de iFluor\u00ae 700., Cat#17530).<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3. <\/strong>An\u00e1lisis de citometr\u00eda de flujo de APC-iFluor\u00ae700 (barra roja) o APC-Alexa Fluor\u00ae 700 (barra azul) anti-CD8 humano en linfocitos humanos. La sangre completa se ti\u00f1\u00f3 con APC-iFluor\u00ae700 o APC-Alexa Fluor\u00ae 700 anti-CD8 humano y se compar\u00f3 con la sangre completa te\u00f1ida con un control de IgG de rat\u00f3n APC-iFluor\u00ae700 y APC-Alexa Fluor\u00ae 700. La citometr\u00eda de flujo se realiz\u00f3 en un sistema de citometr\u00eda de flujo ACEA.<\/p>\n\n\n\n

<\/div>\n\n\n\n

<\/p>\n\n\n\n

Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification<\/a>
Authors: <\/strong>Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: <\/strong>Metabolomics (Los Angel) (2017): 2153–0769<\/p>\n\n\n\n

Suramin inhibits cullin-RING E3 ubiquitin ligases<\/a>
Authors: <\/strong>Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: <\/strong>Proceedings of the National Academy of Sciences (2016): E2011–E2018<\/p>\n\n\n\n

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function<\/a>
Authors: <\/strong>Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: <\/strong>International Journal of Cancer (2012): E425–E436<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 49 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: <\/strong>Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: <\/strong>Mol Biosyst (2011): 1766<\/p>\n\n\n\n

Visualizing dengue virus through Alexa Fluor labeling
Authors: <\/strong>Zhang S, Tan HC, Ooi EE.
Journal: <\/strong>J Vis Exp. (2011)<\/p>\n\n\n\n

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: <\/strong>Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: <\/strong>Biochem Biophys Res Commun (2011): 211<\/p>\n\n\n\n

Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: <\/strong>Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: <\/strong>Zhen Ci Yan Jiu (2011): 262<\/p>\n\n\n\n

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: <\/strong>Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: <\/strong>J Microbiol Methods (2011): 119<\/p>\n\n\n\n

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: <\/strong>Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: <\/strong>J Neurochem (2010): 994<\/p>\n\n\n\n

Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: <\/strong>Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: <\/strong>Org Biomol Chem (2010): 4249<\/p>\n\n\n\n

Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: <\/strong>Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: <\/strong>Zhen Ci Yan Jiu (2010): 433<\/p>\n\n\n\n

Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: <\/strong>Maurel D, Banala S, Laroche T, Johnsson K.
Journal: <\/strong>ACS Chem Biol (2010): 507<\/p>\n\n\n\n

Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: <\/strong>Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: <\/strong>Biochem Pharmacol (2010): 506<\/p>\n\n\n\n

<\/div>\n\n\n\n
\u2022 Guia de Selecci\u00f3n tintes iFlour\u00ae<\/a><\/mark><\/h5>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

El tinte iFluor\u00ae 700 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n","protected":false},"featured_media":2433,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2420"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":10,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2420\/revisions"}],"predecessor-version":[{"id":3680,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2420\/revisions\/3680"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2433"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2420"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}