{"id":2406,"date":"2022-12-28T16:58:07","date_gmt":"2022-12-28T22:58:07","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2406"},"modified":"2023-01-20T11:39:50","modified_gmt":"2023-01-20T17:39:50","slug":"ifluor-647-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-647-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 647 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). Los colorantes iFluor\u00ae 700 tienen un m\u00e1ximo de excitaci\u00f3n y emisi\u00f3n de fluorescencia de ~690 nm y ~713 nm respectivamente. Estas caracter\u00edsticas espectrales los convierten en una excelente alternativa al colorante de etiquetado Alexa Fluor\u00ae 700 (Alexa Fluor\u00ae es la marca comercial de Invitrogen). iFluor\u00ae 700 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1031<\/td>iFluor\u00ae 647 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71031<\/td>iFluor\u00ae 647 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71510<\/td>iFluor\u00ae 647 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71560<\/td>iFluor\u00ae 647 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1274.66<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.03<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.03<\/td><\/tr>
Factor de correcci\u00f3n (656 nm)<\/td>0.0793<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2500001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>656<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>670<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.251<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 700 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

 <\/td>0.1 mg<\/strong><\/strong><\/td>0.5 mg<\/strong><\/strong><\/td>1 mg<\/strong><\/strong><\/td>5 mg<\/strong><\/strong><\/td>10 mg<\/strong><\/strong><\/td><\/tr>
1 mM<\/strong><\/strong><\/td>78.452 \u00b5L<\/td>392.261 \u00b5L<\/td>784.523 \u00b5L<\/td>3.923 mL<\/td>7.845 mL<\/td><\/tr>
5 mM<\/strong><\/strong><\/td>15.69 \u00b5L<\/td>78.452 \u00b5L<\/td>156.905 \u00b5L<\/td>784.523 \u00b5L<\/td>1.569 mL<\/td><\/tr>
10 mM<\/strong><\/strong><\/td>7.845 \u00b5L<\/td>39.226 \u00b5L<\/td>78.452 \u00b5L<\/td>392.261 \u00b5L<\/td>784.523 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.  <\/strong>Las c\u00e9lulas HeLa se ti\u00f1eron con antitubulina de rat\u00f3n seguido de iFluorTM 647 de cabra anti-rat\u00f3n IgG (H+L) (rojo); y los n\u00facleos se ti\u00f1eron con DAPI (azul).<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2<\/strong>. <\/strong>Las c\u00e9lulas HeLa se incubaron con antitubulina de rat\u00f3n seguida de conjugado de IgG anti-rat\u00f3n de cabra iFluorTM 647 de AAT (rojo, izquierda) o IgG anti-rat\u00f3n de cabra Alexa Fluor\u00ae 647 (rojo, derecha), respectivamente. Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (Blue, Cat#17530).<\/p>\n\n\n\n

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Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3<\/strong>  Las c\u00e9lulas HeLa se incubaron con antitubulina de rat\u00f3n y anti-IgG de rat\u00f3n de cabra con biotina, seguidas de conjugado iFluor\u00ae 647-estreptavidina de AAT (rojo, izquierda) o estreptavidina conjugada con Alexa Fluor\u00ae 647 (rojo, derecha), respectivamente.<\/p>\n\n\n\n

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Fig 4.<\/figcaption><\/figure>\n\n\n\n

Figura 4.<\/strong>  Hibridaci\u00f3n fluorescente in situ de sondas de tel\u00f3mero marcadas con Cy5 e iFlour\u00ae 647-dUTP en celulas HeLa en Metafase.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

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Inhibition of MYC suppresses programmed cell death ligand-1 expression and enhances immunotherapy in triple-negative breast cancer<\/a>
Authors: <\/strong>Li, Xintong and Tang, Lin and Chen, Qin and Cheng, Xumin and Liu, Yiqiu and Wang, Cenzhu and Zhu, Chengjun and Xu, Kun and Gao, Fangyan and Huang, Jinyi and others,
Journal: <\/strong>Chinese Medical Journal (2022): 10–1097<\/p>\n\n\n\n

Immune-regulating bimetallic metal-organic framework nanoparticles designed for cancer immunotherapy<\/a>
Authors: <\/strong>Dai, Zan and Wang, Qiaoyun and Tang, Jie and Wu, Min and Li, Haoze and Yang, Yannan and Zhen, Xu and Yu, Chengzhong
Journal: <\/strong>Biomaterials (2022): 121261<\/p>\n\n\n\n

Preparation and identification of monoclonal antibodies against porcine CD103<\/a>
Authors: <\/strong>Zhang, Tao and Yu, Haoyuan and Aryal, Manita and Yang, Jing and Li, Maolin and Li, Shuxian and Zhang, Na and Shi, Han and Li, Baoyu and Liu, Guangliang and others,
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Site-specific labeling and functional efficiencies of human fibroblast growth Factor-1 with a range of fluorescent Dyes in the flexible N-Terminal region and a rigid $\\beta$-turn region<\/a>
Authors: <\/strong>Mohale, Mamello and Gundampati, Ravi Kumar and Kumar, Thallapuranam Krishnaswamy Suresh and Heyes, Colin D
Journal: <\/strong>Analytical biochemistry (2022): 114524<\/p>\n\n\n\n

A nano-innate immune system activator for cancer therapy in a 4T1 tumor-bearing mouse model<\/a>
Authors: <\/strong>Liu, Xiang-Yu and Zhu, Mao-Hua and Wang, Xiao-Yu and Dong, Xiao and Liu, Hai-Jun and Li, Rui-Yang and Jia, Shi-Chong and Lu, Qin and Zhao, Mei and Sun, Peng and others,
Journal: <\/strong>Journal of nanobiotechnology (2022): 1–15<\/p>\n\n\n\n

Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genome<\/a>
Authors: <\/strong>Est{\\`e}ve, Pierre-Olivier and Sen, Sagnik and Vishnu, Udayakumar S and Ruse, Cristian and Chin, Hang Gyeong and Pradhan, Sriharsa
Journal: <\/strong>Communications biology (2022): 1–18<\/p>\n\n\n\n

The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma Effects<\/a>
Authors: <\/strong>Shen, Hong-Yuan and Tang, Hai-Long and Zheng, Yan-Hua and Feng, Juan and Dong, Bao-Xia and Chen, Xie-Qun
Journal: <\/strong>Journal of Oncology (2022)<\/p>\n\n\n\n

A Substance P (SP)\/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641<\/a>
Authors: <\/strong>Ji, Tengfei and Ma, Keqiang and Wu, Hongsheng and Cao, Tiansheng
Journal: <\/strong>Journal of Immunology Research (2022)<\/p>\n\n\n\n

RNA-Editing Enzyme ADAR1 p150 Isoform Is Critical for Germinal Center B Cell Response<\/a>
Authors: <\/strong>Li, Yuxing and Ruan, Gui-Xin and Chen, Wenjing and Huang, Hengjun and Zhang, Rui and Wang, Jing and Li, Yan and Xu, Shengli and Ou, Xijun
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Increased maternofoetal transfer of antibodies in a murine model of systemic lupus erythematosus, but no immune activation and neuroimmune sequelae in offspring<\/a>
Authors: <\/strong>Fonager, Sofie Vestergaard and Winther, Gudrun and Wittenborn, Thomas Rea and Jensen, Lisbeth and Fahlquist-Hagert, Cecilia and Hansen, Lisbeth Ahm and F{\\”u}chtbauer, Ernst-Martin and Romero-Ramos, Marina and Degn, S{\\o}ren Egedal
Journal: <\/strong>Journal of Neuroimmunology (2022)<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n


FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a>
iFluor\u00ae Dye Selection Guide<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

iFluor\u00ae 647 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n","protected":false},"featured_media":2408,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2406"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2406\/revisions"}],"predecessor-version":[{"id":3682,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2406\/revisions\/3682"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2408"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2406"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}