{"id":2389,"date":"2022-12-27T17:13:39","date_gmt":"2022-12-27T23:13:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2389"},"modified":"2023-01-20T11:43:21","modified_gmt":"2023-01-20T17:43:21","slug":"ifluor-555-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-555-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 555 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). Los colorantes iFluor\u00ae 555 tienen un m\u00e1ximo de excitaci\u00f3n y emisi\u00f3n de fluorescencia de ~557 nm y ~570 nm respectivamente. La familia iFluor\u00ae 555 tiene propiedades espectrales esencialmente id\u00e9nticas a las de Cy3\u00ae (Cy3\u00ae es una marca comercial de GE Healthcare). <\/p>\n\n\n\n

En comparaci\u00f3n con las sondas Cy3, los reactivos iFluor\u00ae 555 tienen una fluorescencia mucho m\u00e1s fuerte y una mayor fotoestabilidad. Su fluorescencia es independiente del pH entre pH 3 y 11. Estas caracter\u00edsticas espectrales hacen de esta nueva familia de colorantes una alternativa superior a Cy3\u00ae. La familia iFluor\u00ae 555 se ha convertido en un excelente reemplazo para el colorante de etiquetado Cy3\u00ae y Alexa Fluor\u00ae 555 (Cy3\u00ae y Alexa Fluor\u00ae son marcas comerciales de Invitrogen y GE Health Care). <\/p>\n\n\n\n

iFluor\u00ae 555 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1028<\/td>iFluor\u00ae 555 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71028<\/td>iFluor\u00ae 555 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71507<\/td>iFluor\u00ae 555 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71557<\/td>iFluor\u00ae 555 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

\"pdf\"SDS<\/a> \"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1125.26<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.23<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.14<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1000001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>557<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>570<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.641<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 555 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>88.868 \u00b5L<\/td>444.342 \u00b5L<\/td>888.684 \u00b5L<\/td>4.443 mL<\/td>8.887 mL<\/td><\/tr>
5 mM<\/strong><\/td>17.774 \u00b5L<\/td>88.868 \u00b5L<\/td>177.737 \u00b5L<\/td>888.684 \u00b5L<\/td>1.777 mL<\/td><\/tr>
10 mM<\/strong><\/td>8.887 \u00b5L<\/td>44.434 \u00b5L<\/td>88.868 \u00b5L<\/td>444.342 \u00b5L<\/td>888.684 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.  <\/strong>Las c\u00e9lulas HeLa se incubaron con antitubulina de rat\u00f3n seguida de conjugado de IgG anti-rat\u00f3n de cabra iFluorTM 555 de AAT (rojo, derecha) o IgG anti-rat\u00f3n de cabra conjugado con Alexa Fluor\u00ae 555 (rojo, izquierda), respectivamente. Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (Blue, Cat# 17530).<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2<\/strong>.  Las c\u00e9lulas HeLa se ti\u00f1eron con antitubulina de rat\u00f3n seguido de iFluorTM 555 de cabra anti-IgG de rat\u00f3n (H+L) (rojo); los filamentos de actina se ti\u00f1eron con conjugado Phalloidin-iFluorTM 488 (verde); y los n\u00facleos se ti\u00f1eron con DAPI (azul).<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n\n\n\n

Bibliograf\u00edas<\/mark><\/p>\n\n\n\n

Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly<\/a>
Authors: <\/strong>De Munter, Stijn and Van Parys, Alexander and Bral, Layla and Ingels, Joline and Goetgeluk, Glenn and Bonte, Sarah and Pille, Melissa and Billiet, Lore and Weening, Karin and Verhee, Annick and others,
Journal: <\/strong>International journal of molecular sciences (2020): 883<\/p>\n\n\n\n

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification<\/a>
Authors: <\/strong>Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: <\/strong>Metabolomics (Los Angel) (2017): 2153–0769<\/p>\n\n\n\n

Suramin inhibits cullin-RING E3 ubiquitin ligases<\/a>
Authors: <\/strong>Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: <\/strong>Proceedings of the National Academy of Sciences (2016): E2011–E2018<\/p>\n\n\n\n

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function<\/a>
Authors: <\/strong>Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: <\/strong>International Journal of Cancer (2012): E425–E436<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las\u00a049 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: <\/strong>Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: <\/strong>Mol Biosyst (2011): 1766<\/p>\n\n\n\n

Visualizing dengue virus through Alexa Fluor labeling
Authors: <\/strong>Zhang S, Tan HC, Ooi EE.
Journal: <\/strong>J Vis Exp. (2011)<\/p>\n\n\n\n

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: <\/strong>Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: <\/strong>Biochem Biophys Res Commun (2011): 211<\/p>\n\n\n\n

Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: <\/strong>Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: <\/strong>Zhen Ci Yan Jiu (2011): 262<\/p>\n\n\n\n

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: <\/strong>Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: <\/strong>J Microbiol Methods (2011): 119<\/p>\n\n\n\n

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: <\/strong>Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: <\/strong>J Neurochem (2010): 994<\/p>\n\n\n\n

Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: <\/strong>Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: <\/strong>Org Biomol Chem (2010): 4249<\/p>\n\n\n\n

Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: <\/strong>Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: <\/strong>Zhen Ci Yan Jiu (2010): 433<\/p>\n\n\n\n

Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: <\/strong>Maurel D, Banala S, Laroche T, Johnsson K.
Journal: <\/strong>ACS Chem Biol (2010): 507<\/p>\n\n\n\n

Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: <\/strong>Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: <\/strong>Biochem Pharmacol (2010): 506<\/p>\n\n\n\n

Ver todas las 49 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n
Guia para tintes iFlour<\/mark><\/h5>\n\n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

iFluor\u00ae 555 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n","protected":false},"featured_media":2393,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2389"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2389\/revisions"}],"predecessor-version":[{"id":3684,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2389\/revisions\/3684"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2393"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2389"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}