{"id":2374,"date":"2022-12-27T16:12:03","date_gmt":"2022-12-27T22:12:03","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2374"},"modified":"2023-03-10T21:34:45","modified_gmt":"2023-03-11T03:34:45","slug":"ifluor-488-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-488-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 488 succinimidyl ester"},"content":{"rendered":"\n

Aunque FITC sigue siendo el tinte marcador fluorescente m\u00e1s popular para la preparaci\u00f3n de bioconjugados fluorescentes verdes, existen ciertas limitaciones con FITC, como fotoblanqueo severo para im\u00e1genes microsc\u00f3picas y fluorescencia sensible al pH. derivados de fluoresce\u00edna como FITC. Los conjugados iFluor\u00ae 488 son significativamente m\u00e1s brillantes que los conjugados de fluoresce\u00edna y son mucho m\u00e1s fotoestables. Adem\u00e1s, la fluorescencia de iFluor\u00ae 488 no se ve afectada por el pH (4-10), que emite su m\u00e1xima fluorescencia solo a pH por encima de 9. <\/p>\n\n\n\n

El tinte iFluor\u00ae 488 SE es razonablemente estable y muestra una buena reactividad y selectividad con los grupos amino de la prote\u00edna. Este iFluor\u00ae 488 tiene propiedades espectrales y reactividad similares al \u00e9ster Alexa Fluor\u00ae 488 NHS (Alexa Fluor\u00ae es la marca comercial de Invitrogen).<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1023<\/td>iFluor\u00ae 488 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71023<\/td>iFluor\u00ae 488 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71503<\/td>iFluor\u00ae 488 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71553<\/td>iFluor\u00ae 488 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

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\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>945.07<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.21<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.11<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>750001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>491<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>516<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.91<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 488 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>105.812 \u00b5L<\/td>529.061 \u00b5L<\/td>1.058 mL<\/td>5.291 mL<\/td>10.581 mL<\/td><\/tr>
5 mM<\/strong><\/td>21.162 \u00b5L<\/td>105.812 \u00b5L<\/td>211.625 \u00b5L<\/td>1.058 mL<\/td>2.116 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.581 \u00b5L<\/td>52.906 \u00b5L<\/td>105.812 \u00b5L<\/td>529.061 \u00b5L<\/td>1.058 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HeLa se ti\u00f1eron con antitubulina de conejo seguida de iFluor 488 de cabra anti-IgG de conejo (H+L), y los n\u00facleos se ti\u00f1eron con Nuclear Red DCS1.<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2<\/strong>. An\u00e1lisis de citometr\u00eda de flujo de PBMC te\u00f1idas con iFluor\u00ae 488 conjugado anti-CD24 humano *HI45*. La se\u00f1al de fluorescencia se control\u00f3 utilizando un cit\u00f3metro de flujo Aurora en el canal B2-A espec\u00edfico de iFluor\u00ae 488.<\/p>\n\n\n\n

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Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Hibridaci\u00f3n in situ con fluorescencia de sondas de tel\u00f3mero marcadas con fluoresce\u00edna y iFluor\u00ae 488 en c\u00e9lulas HeLa en metafase.<\/p>\n\n\n\n

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Fig. 4<\/figcaption><\/figure>\n\n\n\n

Figura 4.<\/strong> An\u00e1lisis de citometr\u00eda de flujo de Alexa Fluor\u00ae 488 o iFluor\u00ae 488 anti-CD4 humano en linfocitos humanos. Las c\u00e9lulas de PBMC se ti\u00f1eron con 0,5 ug de Alexa Fluor\u00ae 488 anti-CD4 humano o 0,5 ug de iFluor\u00ae 488 anti-CD4 humano en cada prueba. El an\u00e1lisis de citometr\u00eda de flujo se realiz\u00f3 en un sistema de citometr\u00eda de flujo ACEA.<\/p>\n\n\n\n

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Fig. 5<\/figcaption><\/figure>\n\n\n\n

Figura 5<\/strong>. Las c\u00e9lulas HeLa se incubaron con (Tubulin+) o sin (Tubulin-) antitubulina de rat\u00f3n seguido de iFluor\u00ae 488 conjugado de IgG anti-rat\u00f3n de cabra (Verde, izquierda) o Alexa Fluor\u00ae 488 conjugado de IgG anti-rat\u00f3n de cabra (Verde , Derecha), respectivamente. Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (azul).<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 32 bibliograf\u00edas: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K\/Akt pathway<\/a>
Authors: <\/strong>Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: <\/strong>Bioengineered (2022): 8772–8784<\/p>\n\n\n\n

Evaluation of Usnea barbata (L.) Weber ex FH Wigg Extract in Canola Oil Loaded in Bioadhesive Oral Films for Potential Applications in Oral Cavity Infections and Malignancy<\/a>
Authors: <\/strong>Popovici, Violeta and Matei, Elena and Cozaru, Georgeta Camelia and Bucur, Laura and G{\\^\\i}rd, Cerasela Elena and Schr{\\”o}der, Verginica and Ozon, Emma Adriana and Karampelas, Oana and Musuc, Adina Magdalena and Atkinson, Irina and others,
Journal: <\/strong>Antioxidants (2022): 1601<\/p>\n\n\n\n

Independent phenotypic plasticity axes define distinct obesity sub-types<\/a>
Authors: <\/strong>Yang, Chih-Hsiang and Fagnocchi, Luca and Apostle, Stefanos and Wegert, Vanessa and Casan{\\’\\i}-Gald{\\’o}n, Salvador and Landgraf, Kathrin and Panzeri, Ilaria and Dror, Erez and Heyne, Steffen and W{\\”o}rpel, Till and others,
Journal: <\/strong>Nature metabolism (2022): 1150–1165<\/p>\n\n\n\n

ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection<\/a>
Authors: <\/strong>Zhu, Xinyu and Trimarco, Joseph D and Williams, Courtney A and Barrera, Alejandro and Reddy, Timothy E and Heaton, Nicholas S
Journal: <\/strong>Cell Reports (2022): 111540<\/p>\n\n\n\n

Osteoclasts directly influence castration-resistant prostate cancer cells<\/a>
Authors: <\/strong>Huang, Junchi and Freyhult, Eva and Buckland, Robert and Josefsson, Andreas and Damber, Jan-Erik and Wel{\\’e}n, Karin
Journal: <\/strong>Clinical \\& experimental metastasis (2022): 801–814<\/p>\n\n\n\n

Phase separation of insulin receptor substrate 1 drives the formation of insulin\/IGF-1 signalosomes<\/a>
Authors: <\/strong>Gao, Xiu Kui and Rao, Xi Sheng and Cong, Xiao Xia and Sheng, Zu Kang and Sun, Yu Ting and Xu, Shui Bo and Wang, Jian Feng and Liang, Yong Heng and Lu, Lin Rong and Ouyang, Hongwei and others,
Journal: <\/strong>Cell discovery (2022): 1–19<\/p>\n\n\n\n

Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock<\/a>
Authors: <\/strong>Mo, Weiliang and Zhang, Junchuan and Zhang, Li and Yang, Zhenming and Yang, Liang and Yao, Nan and Xiao, Yong and Li, Tianhong and Li, Yaxing and Zhang, Guangmei and others,
Journal: <\/strong>Nature communications (2022): 1–15<\/p>\n\n\n\n

Arc weakens synapses by dispersing AMPA receptors from postsynaptic density via modulating PSD phase separation<\/a>
Authors: <\/strong>Chen, Xudong and Jia, Bowen and Araki, Yoichi and Liu, Bian and Ye, Fei and Huganir, Richard and Zhang, Mingjie
Journal: <\/strong>Cell Research (2022): 914–930<\/p>\n\n\n\n

A Novel CDK4\/6 and PARP Dual Inhibitor ZC-22 Effectively Suppresses Tumor Growth and Improves the Response to Cisplatin Treatment in Breast and Ovarian Cancer<\/a>
Authors: <\/strong>Tian, Chenchen and Wei, Yufan and Li, Jianjun and Huang, Zhi and Wang, Qiong and Lin, Yingxue and Lv, Xingping and Chen, Yanan and Fan, Yan and Sun, Peiqing and others,
Journal: <\/strong>International journal of molecular sciences (2022): 2892<\/p>\n\n\n\n

p73-regulated FER1L4 lncRNA sponges the oncogenic potential of miR-1273g-3p and aids in the suppression of colorectal cancer metastasis<\/a>
Authors: <\/strong>Uboveja, Apoorva and Satija, Yatendra Kumar and Siraj, Fouzia and Saluja, Daman
Journal: <\/strong>Iscience (2022): 103811<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

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Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
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Visualizing dengue virus through Alexa Fluor labeling
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Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
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Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
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Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
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Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
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Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
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Application Notes <\/mark><\/p>\n\n\n\n

FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a>
iFluor\u00ae Dye Selection Guide<\/a>
Introducing Calbryte\u2122 Series<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are common laser lines used in flow cytometry?<\/a>
What are the spectral properties of iFluor dyes?<\/a>
Why should isotype controls be used in immunofluorescence staining?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este iFluor\u00ae 488 tiene propiedades espectrales y reactividad similares al \u00e9ster Alexa Fluor\u00ae 488 NHS (Alexa Fluor\u00ae es una marca comercial de Invitrogen).<\/p>\n","protected":false},"featured_media":2376,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2374"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":11,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2374\/revisions"}],"predecessor-version":[{"id":8355,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2374\/revisions\/8355"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2376"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2374"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2374"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}