{"id":2358,"date":"2022-12-27T10:28:43","date_gmt":"2022-12-27T16:28:43","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2358"},"modified":"2023-01-20T11:53:44","modified_gmt":"2023-01-20T17:53:44","slug":"6-joe-se","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/6-joe-se\/","title":{"rendered":"6-JOE, SE"},"content":{"rendered":"\n

6-JOE tiene espectros de absorci\u00f3n y emisi\u00f3n similares a los de la rodamina 6G, y su SE se usa predominantemente en la secuenciaci\u00f3n autom\u00e1tica de ADN. Tambi\u00e9n se utiliza para marcar nucle\u00f3tidos.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentacion<\/th><\/tr><\/thead>
AAT-203<\/td>6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] <\/td>5mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>602.34<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>750001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>520<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>545<\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer (pH9)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de 6-JOE, SE [6-carboxi-4′,5′-dicloro-2′,7′-dimetoxifluoresce\u00edna, succinimidil \u00e9ster] *CAS#: 113394-23-3 * a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>166.019 \u00b5L<\/td>830.096 \u00b5L<\/td>1.66 mL<\/td>8.301 mL<\/td>16.602 mL<\/td><\/tr>
5 mM<\/strong><\/td>33.204 \u00b5L<\/td>166.019 \u00b5L<\/td>332.038 \u00b5L<\/td>1.66 mL<\/td>3.32 mL<\/td><\/tr>
10 mM<\/strong><\/td>16.602 \u00b5L<\/td>83.01 \u00b5L<\/td>166.019 \u00b5L<\/td>830.096 \u00b5L<\/td>1.66 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Figura 1. <\/figcaption><\/figure>\n\n\n\n

Estructura qu\u00edmica de 6-JOE, SE [6-carboxi-4′,5′-dicloro-2′,7′-dimetoxifluoresce\u00edna, succinimidil \u00e9ster] CAS#: 113394-23-3<\/em><\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Correction Factor (280 nm)<\/td><\/tr>
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.178<\/td><\/tr>
6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*<\/a><\/td>533<\/td>559<\/td>73000<\/td>0.15<\/td><\/tr>
6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester]<\/a><\/td>521<\/td>542<\/td>73000<\/td>0.13<\/td><\/tr>
6-JOE azide<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td><\/tr>
6-JOE alkyne<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td><\/tr>
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*<\/a><\/td>522<\/td>546<\/td>94000<\/td>0.214<\/td><\/tr>
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*<\/a><\/td>552<\/td>578<\/td>90000<\/td>0.178<\/td><\/tr>
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-ROXtra\u2122 SE *Superior 6-ROX Replacement*<\/a><\/td>578<\/td>595<\/td>–<\/td>–<\/td><\/tr>
6-VIC, SE [6-VIC NHS ester]<\/a><\/td>526<\/td>543<\/td>–<\/td>–<\/td><\/tr>
6-JOE Phosphoramidite<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td><\/tr>
6-NED, SE [6-NED NHS ester]<\/a><\/td>545<\/td>567<\/td>740001<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer (pH9)<\/figcaption><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors:\u00a0<\/strong>Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal:\u00a0<\/strong>J Clin Microbiol\u00a0(2005):\u00a02058<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease <\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/mark><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

[6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*<\/p>\n","protected":false},"featured_media":2360,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2358"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":9,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2358\/revisions"}],"predecessor-version":[{"id":3690,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2358\/revisions\/3690"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2360"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2358"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2358"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}