{"id":2349,"date":"2022-12-26T17:31:02","date_gmt":"2022-12-26T23:31:02","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2349"},"modified":"2023-01-20T11:56:33","modified_gmt":"2023-01-20T17:56:33","slug":"6-hex-se","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/6-hex-se\/","title":{"rendered":"6-HEX, SE"},"content":{"rendered":"\n

6-HEX, SE es el \u00e9ster succinimidilo reactivo con amina del \u00e1cido 6-HEX. Es ampliamente utilizado en la secuenciaci\u00f3n de \u00e1cidos nucleicos e investigaciones relacionadas.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentacion<\/th><\/tr><\/thead>
AAT-202<\/td>6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*<\/td>5mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelacion (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>680.06<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de Correcci\u00f3n (280 nm)<\/td>0.15<\/td><\/tr>
Coeficiente de Extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>73000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>533<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>559<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de 6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexaclorofluoresce\u00edna, succinimidil \u00e9ster] *Is\u00f3mero \u00fanico* a la concentraci\u00f3n dada . Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>147.046 \u00b5L<\/td>735.229 \u00b5L<\/td>1.47 mL<\/td>7.352 mL<\/td>14.705 mL<\/td><\/tr>
5 mM<\/strong><\/td>29.409 \u00b5L<\/td>147.046 \u00b5L<\/td>294.092 \u00b5L<\/td>1.47 mL<\/td>2.941 mL<\/td><\/tr>
10 mM<\/strong><\/td>14.705 \u00b5L<\/td>73.523 \u00b5L<\/td>147.046 \u00b5L<\/td>735.229 \u00b5L<\/td>1.47 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Figura 1. <\/figcaption><\/figure>\n\n\n\n

Estructura qu\u00edmica de 6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexaclorofluoresce\u00edna, succinimidil \u00e9ster] *Is\u00f3mero \u00fanico<\/em>*<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Correction Factor (280 nm)<\/td><\/tr>
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.178<\/td><\/tr>
6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td><\/tr>
6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester]<\/a><\/td>521<\/td>542<\/td>73000<\/td>0.13<\/td><\/tr>
6-HEX azide<\/a><\/td>533<\/td>559<\/td>73000<\/td>0.15<\/td><\/tr>
6-HEX alkyne<\/a><\/td>533<\/td>559<\/td>73000<\/td>0.15<\/td><\/tr>
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*<\/a><\/td>522<\/td>546<\/td>94000<\/td>0.214<\/td><\/tr>
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*<\/a><\/td>552<\/td>578<\/td>90000<\/td>0.178<\/td><\/tr>
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite]<\/a><\/td>533<\/td>559<\/td>73000<\/td>0.15<\/td><\/tr>
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1<\/sup> Buffer pH9<\/figcaption><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

DNaasikontaminaation m{\\”a}{\\”a}ritysmenetelm{\\”a}n karakterisointi
Authors:\u00a0<\/strong>Sn{\\”a}ll, Juho
Journal:\u00a0<\/strong>(2019)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis
Authors: <\/strong>Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F.
Journal: <\/strong>Mol Vis (2006): 1098<\/p>\n\n\n\n

Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues
Authors: <\/strong>Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D.
Journal: <\/strong>Plant J (2004): 947<\/p>\n\n\n\n

Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography
Authors: <\/strong>Fountain KJ, Gilar M, Budman Y, Gebler JC.
Journal: <\/strong>J Chromatogr B Analyt Technol Biomed Life Sci (2003): 61<\/p>\n\n\n\n

Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
Authors: <\/strong>Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC.
Journal: <\/strong>J Clin Microbiol (2003): 4016<\/p>\n\n\n\n

Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis
Authors: <\/strong>Iwamoto T, Sonobe T, Hayashi K.
Journal: <\/strong>J Clin Microbiol (2002): 4705<\/p>\n\n\n\n

Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Authors: <\/strong>Simeonov A, Nikiforov TT.
Journal: <\/strong>Nucleic Acids Res (2002): e91<\/p>\n\n\n\n

Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization
Authors: <\/strong>Reid SL, Parry D, Liu HH, Connolly BA.
Journal: <\/strong>Biochemistry (2001): 2484<\/p>\n\n\n\n

Strand-separating activity of hepatitis C virus helicase in the absence of ATP
Authors: <\/strong>Porter DJ, Preugschat F.
Journal: <\/strong>Biochemistry (2000): 5166<\/p>\n\n\n\n

Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
Authors: <\/strong>Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L.
Journal: <\/strong>J Clin Microbiol (1999): 1941<\/p>\n\n\n\n

Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA
Authors: <\/strong>Gumbs OH, Shaner SL.
Journal: <\/strong>Biochemistry (1998): 11692<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease <\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

[6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*<\/p>\n","protected":false},"featured_media":2351,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2349"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":8,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2349\/revisions"}],"predecessor-version":[{"id":3692,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2349\/revisions\/3692"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2351"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2349"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2349"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}