{"id":2292,"date":"2022-12-23T16:58:00","date_gmt":"2022-12-23T22:58:00","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2292"},"modified":"2023-01-20T12:12:10","modified_gmt":"2023-01-20T18:12:10","slug":"cyanine-5-5-bissuccinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cyanine-5-5-bissuccinimidyl-ester\/","title":{"rendered":"Cyanine 5.5 bissuccinimidyl ester"},"content":{"rendered":"\n

Se han utilizado una variedad de tintes de cianina 5.5 (Cy5.5\u00ae) para marcar mol\u00e9culas biol\u00f3gicas para im\u00e1genes de fluorescencia y otros an\u00e1lisis bioqu\u00edmicos basados en fluorescencia. Se utilizan ampliamente para marcar p\u00e9ptidos, prote\u00ednas y oligos, etc. Los tintes Cy5.5\u00ae son un tipo de los fluor\u00f3foros rojos m\u00e1s comunes. El \u00e9ster Cy5.5\u00ae NHS reacciona f\u00e1cilmente con los grupos amino. <\/p>\n\n\n\n

AAT Bioquest ofrece \u00e9steres de colorante Cy NHS en forma de sales de trietilamonio que son m\u00e1s solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados id\u00e9nticos a las sales de potasio del tinte Cy. Cy5.5\u00ae es una marca comercial de GE Healthcare.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentacion<\/th><\/tr><\/thead>
AAT-158<\/td>Cyanine 5.5 bissuccinimidyl ester<\/td>1mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a> <\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelacion (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas <\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1500.87<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de Correcci\u00f3n (260 nm)<\/td>0.05<\/td><\/tr>
Factor de Correcci\u00f3n (280 nm)<\/td>0.101<\/td><\/tr>
Factor de Correcci\u00f3n (482 nm)<\/td>0.0017<\/td><\/tr>
Factor de Correcci\u00f3n (565 nm)<\/td>0.047<\/td><\/tr>
Factor de Correcci\u00f3n (650 nm)<\/td>0.454<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>250000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>683<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>703<\/td><\/tr>
Rendimiento Cu\u00e1ntico<\/td>0.27<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de Cyanine 5.5 bissuccinimidil \u00e9ster [equivalente a Cy5.5\u00ae bisNHS \u00e9ster] a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>66.628 \u00b5L<\/td>333.14 \u00b5L<\/td>666.28 \u00b5L<\/td>3.331 mL<\/td>6.663 mL<\/td><\/tr>
5 mM<\/strong><\/td>13.326 \u00b5L<\/td>66.628 \u00b5L<\/td>133.256 \u00b5L<\/td>666.28 \u00b5L<\/td>1.333 mL<\/td><\/tr>
10 mM<\/strong><\/td>6.663 \u00b5L<\/td>33.314 \u00b5L<\/td>66.628 \u00b5L<\/td>333.14 \u00b5L<\/td>666.28 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Figura 1. <\/figcaption><\/figure>\n\n\n\n

Estructura qu\u00edmica del \u00e9ster de bissuccinimidilo de cianina 5.5 [equivalente al \u00e9ster de bisNHS de Cy5.5\u00ae]<\/p>\n\n\n\n

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Producto Similar<\/mark><\/p>\n\n\n\n

Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td>Correction Factor (482 nm)<\/td>Correction Factor (565 nm)<\/td>Correction Factor (650 nm)<\/td><\/tr>
Cyanine 5.5 monosuccinimidyl ester [equivalent to Cy5.5\u00ae NHS ester]<\/a><\/td>683<\/td>703<\/td>250000<\/td>0.27<\/td>0.05<\/td>0.101<\/td>0.0017<\/td>0.047<\/td>0.454<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization<\/a>
Authors: <\/strong>Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: <\/strong>Journal of Biomaterials Applications (2017): 923–932<\/p>\n\n\n\n

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy<\/a>
Authors: <\/strong>Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2017)<\/p>\n\n\n\n

Light\/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics<\/a>
Authors: <\/strong>Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: <\/strong>Journal of Controlled Release (2017)<\/p>\n\n\n\n

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction<\/a>
Authors: <\/strong>Liu, Cuiping and Stonestrom, Aaron J and Christian, Thomas and Yong, Jeongsik and Takase, Ryuichi and Hou, Ya-Ming and Yang, Xiaolu
Journal: <\/strong>Journal of Biological Chemistry (2016): 10426–10436<\/p>\n\n\n\n

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules<\/a>
Authors: <\/strong>Ngo, John T and Adams, Stephen R and Deerinck, Thomas J and Boassa, Daniela and Rodriguez-Rivera, Frances and Palida, Sakina F and Bertozzi, Carolyn R and Ellisman, Mark H and Tsien, Roger Y
Journal: <\/strong>Nat Chem Biol (2016): 459–465<\/p>\n\n\n\n

Design, synthesis and evaluation of VEGF-siRNA\/CRS as a novel vector for gene delivery<\/a>
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Journal: <\/strong>Drug Design, Development and Therapy (2016): 3851<\/p>\n\n\n\n

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions<\/a>
Authors: <\/strong>Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao
Journal: <\/strong>Biomaterials (2016)<\/p>\n\n\n\n

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy<\/a>
Authors: <\/strong>Deng, Xiongwei and Yin, Zhaoxia and Zhou, Zhixiang and Wang, Yihui and Zhang, Fang and Hu, Qin and Yang, Yishu and Lu, Jianqing and Wu, Yan and Sheng, Wang and others, undefined
Journal: <\/strong>ACS applied materials & interfaces (2016): 17068–17079<\/p>\n\n\n\n

Multiplexed single-cell in situ RNA analysis by reiterative hybridization<\/a>
Authors: <\/strong>Xiao, Lu and Guo, Jia
Journal: <\/strong>Analytical Methods (2015): 7290–7295<\/p>\n\n\n\n

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography<\/a>
Authors: <\/strong>Nagamine, Takeaki and Hayakawa, Kou and Nakazato, Kyoumi and Iha, Masahiko
Journal: <\/strong>Journal of Chromatography B (2015): 187–193<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
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Journal: <\/strong>Appl Phys Lett (2011): 143703<\/p>\n\n\n\n

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: <\/strong>Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: <\/strong>Theranostics (2011): 381<\/p>\n\n\n\n

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
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Journal: <\/strong>Biochemistry (2011): 2691<\/p>\n\n\n\n

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
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Journal: <\/strong>JACC Cardiovasc Imaging (2009): 1114<\/p>\n\n\n\n

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
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Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
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Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
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Cy7-Bis-dipicolylamine-zinc
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Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: <\/strong>Cheng KT., undefined
Journal: <\/strong>In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptide<\/a>s<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications <\/a>In Drug Discovery and Dis<\/a>eas<\/a>e <\/a>Diagnosis<\/a>
FITC (Fluorescein i<\/a>sothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Equivalent a Cy5.5\u00ae bisNHS ester<\/p>\n","protected":false},"featured_media":2300,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2292"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":15,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2292\/revisions"}],"predecessor-version":[{"id":3696,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2292\/revisions\/3696"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2300"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2292"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2292"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}