{"id":2272,"date":"2022-12-23T16:13:16","date_gmt":"2022-12-23T22:13:16","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2272"},"modified":"2023-01-20T12:15:52","modified_gmt":"2023-01-20T18:15:52","slug":"cyanine-5-monosuccinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cyanine-5-monosuccinimidyl-ester\/","title":{"rendered":"Cyanine 5 monosuccinimidyl ester"},"content":{"rendered":"\n

Se han utilizado una variedad de colorantes de cianina 5 (Cy5\u00ae) para marcar mol\u00e9culas biol\u00f3gicas para im\u00e1genes de fluorescencia y otros an\u00e1lisis bioqu\u00edmicos basados \u200b\u200ben fluorescencia. Se utilizan ampliamente para marcar p\u00e9ptidos, prote\u00ednas y oligos, etc. Los tintes Cy5\u00ae son un tipo de \u00a0fluor\u00f3foros rojos m\u00e1s comunes. Estos fluor\u00f3foros vers\u00e1tiles pueden tolerar un rango de pH de 3 a 10 para su uso en una variedad de aplicaciones a pH biol\u00f3gicamente relevantes. Los tintes tambi\u00e9n son tolerantes a DMSO y fotoestables para permitir la transferencia desde el almacenamiento hasta el ensayo sin p\u00e9rdida de rendimiento. La solubilidad acuosa elimina la necesidad de disolventes org\u00e1nicos en los buffers de ensayo. <\/p>\n\n\n\n

Nuestros Cy5\u00ae Fluors se someten a pruebas de control de calidad exhaustivas para garantizar altos niveles de crom\u00f3foro y contenido de colorante reactivo. Los tintes monorreactivos son adecuados para el etiquetado preciso y dirigido de prote\u00ednas y oligonucle\u00f3tidos, y los tintes bioreactivos son m\u00e1s adecuados para el etiquetado general. Se recomiendan colorantes de \u00e9ster NHS para marcar grupos amina y colorantes de maleimida para marcar grupos tiol. <\/p>\n\n\n\n

Este \u00e9ster Cy5\u00ae NHS reacciona f\u00e1cilmente con grupos amino. AAT Bioquest ofrece \u00e9steres de colorante Cy NHS en forma de sales de trietilamonio que son m\u00e1s solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados id\u00e9nticos a las sales de potasio del tinte Cy. Cy5\u00ae es la marca comercial de GE Healthcare<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentacion<\/th><\/tr><\/thead>
AAT-151<\/td>Cyanine 5 monosuccinimidyl ester<\/td>1mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a> <\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

\u00a0Propedades Fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>855.07<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.02<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.03<\/td><\/tr>
Factor de correcci\u00f3n (482 nm)<\/td>0.009<\/td><\/tr>
Factor de correcci\u00f3n (565 nm)<\/td>0.09<\/td><\/tr>
Coeficiente de Extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2500001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>651<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>670<\/td><\/tr>
Rendimiento Cu\u00e1ntico <\/td>0.272<\/sup>, 0.41<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Ethanol, 2<\/sup> PBS <\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica del \u00e9ster monosuccinimid\u00edli de cianina 5 [equivalente al \u00e9ster Cy5\u00ae NHS] a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>116.949 \u00b5L<\/td>584.747 \u00b5L<\/td>1.169 mL<\/td>5.847 mL<\/td>11.695 mL<\/td><\/tr>
5 mM<\/strong><\/td>23.39 \u00b5L<\/td>116.949 \u00b5L<\/td>233.899 \u00b5L<\/td>1.169 mL<\/td>2.339 mL<\/td><\/tr>
10 mM<\/strong><\/td>11.695 \u00b5L<\/td>58.475 \u00b5L<\/td>116.949 \u00b5L<\/td>584.747 \u00b5L<\/td>1.169 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1. <\/strong>Las c\u00e9lulas HeLa se incubaron con (Tubulin+) o sin (Tubulin-) anti-tubulina de rat\u00f3n seguido de conjugado de IgG anti-rat\u00f3n de cabra Cy5\u00ae de AAT (rojo, izquierda) o conjugado de IgG anti-rat\u00f3n de cabra Cy5\u00ae de Jackson (rojo, izquierda). derecha), respectivamente. Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (Blue, Cat# 17530).<\/p>\n\n\n\n

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<\/div>\n\n\n\n
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Figure 2. <\/strong>Estructura Quimica de Cyanine 5 monosuccinimidyl ester [equivalente a Cy5\u00ae NHS ester]<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td>Correction Factor (482 nm)<\/td>Correction Factor (565 nm)<\/td><\/tr>
Cyanine 3 monosuccinimidyl ester [equivalent to Cy3\u00ae NHS ester]<\/a><\/td>555<\/td>569<\/td>1500001<\/sup><\/td>0.151<\/sup><\/td>0.07<\/td>0.073<\/td>–<\/td>–<\/td><\/tr>
Cyanine 5 bissuccinimidyl ester [equivalent to Cy5\u00ae bisNHS ester]<\/a><\/td>651<\/td>670<\/td>2500001<\/sup><\/td>0.271<\/sup>, 0.42<\/sup><\/td>0.02<\/td>0.03<\/td>0.009<\/td>0.09<\/td><\/tr>
Cyanine 7 monosuccinimidyl ester [equivalent to Cy7\u00ae NHS ester]<\/a><\/td>756<\/td>779<\/td>250000<\/td>0.3<\/td>0.05<\/td>0.036<\/td>0.0005<\/td>0.0193<\/td><\/tr><\/tbody><\/table>
1<\/sup> PBS 2<\/sup> Ethanol<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 23 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Phase separation-mediated condensation of Whirlin-Myo15-Eps8 stereocilia tip complex<\/a>
Authors: <\/strong>Lin, Lin and Shi, Yingdong and Wang, Mengli and Wang, Chao and Lu, Qing and Zhu, Jinwei and Zhang, Rongguang
Journal: <\/strong>Cell Reports (2021): 108770<\/p>\n\n\n\n

Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization<\/a>
Authors: <\/strong>Xiao, Lu and Liao, Renjie and Guo, Jia
Journal: <\/strong>Molecules (2020): 4900<\/p>\n\n\n\n

A novel matrix protein PfX regulates shell ultrastructure by binding to specific calcium carbonate crystal faces<\/a>
Authors: <\/strong>Yang, Xue and Yang, Dong and Yan, Yi and Li, Shiguo and Han, Zaiming and Ji, Yinghui and Zheng, Guilan and Xie, Liping and Zhang, Rongqing
Journal: <\/strong>International Journal of Biological Macromolecules (2020)<\/p>\n\n\n\n

A basic protein, N25, from a mollusk modifies calcium carbonate morphology and shell biomineralization<\/a>
Authors: <\/strong>Yang, Dong and Yan, Yi and Yang, Xue and Liu, Jun and Zheng, Guilan and Xie, Liping and Zhang, Rongqing
Journal: <\/strong>Journal of Biological Chemistry (2019): jbc–RA118<\/p>\n\n\n\n

Highly Multiplexed Single Cell In Situ Transcriptomic Analysis.
Authors: <\/strong>Xiao, Lu
Journal: <\/strong>(2019)<\/p>\n\n\n\n

Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed Molecular Assembly via Liquid-Liquid Phase Separation<\/a>
Authors: <\/strong>He, Yunyun and Li, Jianchao and Zhang, Mingjie
Journal: <\/strong>Cell reports (2019): 974–986<\/p>\n\n\n\n

Single-cell in situ RNA analysis with switchable fluorescent oligonucleotides
Authors: <\/strong>Xiao, Lu and Guo, Jia
Journal: <\/strong>Frontiers in cell and developmental biology (2018): 42<\/p>\n\n\n\n

Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference<\/a>
Authors: <\/strong>Li, Jiahe and Wu, Connie and Wang, Wade and He, Yanpu and Elkayam, Elad and Joshua-Tor, Leemor and Hammond, Paula T
Journal: <\/strong>Proceedings of the National Academy of Sciences (2018): E2696–E2705<\/p>\n\n\n\n

Reconstituted postsynaptic density as a molecular platform for understanding synapse formation and plasticity<\/a>
Authors: <\/strong>Zeng, Menglong and Chen, Xudong and Guan, Dongshi and Xu, Jia and Wu, Haowei and Tong, Penger and Zhang, Mingjie
Journal: <\/strong>Cell (2018): 1172–1187<\/p>\n\n\n\n

A rapid test strip for diagnosing glycosylated hemoglobin (HbA1c) based on fluorescent affinity immunochromatography<\/a>
Authors: <\/strong>Ang, Chaoman and Lou, Doudou and Hu, Linling and Chen, Wei and Zhu, Yefei and Guo, Zhirui and Gu, Ning and Zhang, Yu
Journal: <\/strong>Analytical Sciences (2018): 18P135<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las\u00a021 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: <\/strong>Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: <\/strong>Appl Phys Lett (2011): 143703<\/p>\n\n\n\n

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: <\/strong>Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: <\/strong>Theranostics (2011): 381<\/p>\n\n\n\n

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: <\/strong>Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: <\/strong>Biochemistry (2011): 2691<\/p>\n\n\n\n

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: <\/strong>Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: <\/strong>JACC Cardiovasc Imaging (2009): 1114<\/p>\n\n\n\n

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: <\/strong>Volke D, Hoffmann R.
Journal: <\/strong>Electrophoresis (2008): 4516<\/p>\n\n\n\n

Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
Authors: <\/strong>Vareiro MM, Tranchant I, Maplin S, Zak K, Gani MM, Slevin CJ, Hailes HC, Tabor AB, Cameron PJ, Jenkins AT, Williams DE.
Journal: <\/strong>Anal Biochem (2008): 243<\/p>\n\n\n\n

Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
Authors: <\/strong>Menacher F, Rubner M, Berndl S, Wagenknecht HA.
Journal: <\/strong>J Org Chem (2008): 4263<\/p>\n\n\n\n

Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
Authors: <\/strong>Wu Y, Cai W, Chen X.
Journal: <\/strong>Mol Imaging Biol (2006): 226<\/p>\n\n\n\n

Cy7-Bis-dipicolylamine-zinc
Authors: <\/strong>Leung K., undefined
Journal: <\/strong>In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)<\/p>\n\n\n\n

Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: <\/strong>Cheng KT., undefined
Journal: <\/strong>In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease<\/a> <\/a>Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Equivalente a Cy5\u00ae NHS ester<\/p>\n","protected":false},"featured_media":2285,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2272"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":20,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2272\/revisions"}],"predecessor-version":[{"id":3698,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2272\/revisions\/3698"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2285"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2272"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}