{"id":2151,"date":"2022-12-23T11:55:38","date_gmt":"2022-12-23T17:55:38","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=2151"},"modified":"2023-01-20T12:37:21","modified_gmt":"2023-01-20T18:37:21","slug":"cyanine-3-monosuccinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cyanine-3-monosuccinimidyl-ester\/","title":{"rendered":"Cyanine 3 monosuccinimidyl ester"},"content":{"rendered":"\n

Se ha utilizado una variedad de tintes de cianina 3 (Cy3\u00ae) para marcar mol\u00e9culas biol\u00f3gicas para im\u00e1genes de fluorescencia y otros an\u00e1lisis bioqu\u00edmicos basados en fluorescencia. Se utilizan ampliamente para marcar p\u00e9ptidos, prote\u00ednas y oligos, etc. Los tintes Cy3\u00ae tienen una fluorescencia mejorada al unirse a las prote\u00ednas. El \u00e9ster Cy3\u00ae NHS reacciona f\u00e1cilmente con los grupos amino. <\/p>\n\n\n\n

AAT Bioquest ofrece \u00e9steres de colorante Cy NHS en forma de sales de trietilamonio que son m\u00e1s solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados id\u00e9nticos a las sales de potasio del tinte Cy. Cy3\u00ae es la marca registrada de GE Healthcare.<\/p>\n\n\n\n

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Cat\u00e1logo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-141<\/td>Cyanine 3 monosuccinimidyl ester<\/td>1mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"SDS<\/a> \"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelacion (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular<\/td>829.03<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.07<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.073<\/td><\/tr>
Coeficiente extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1500001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>555<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>569<\/td><\/tr>
Rendimiento Cu\u00e1ntico<\/td>0.151<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> PBS<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica del \u00e9ster monosuccinimid\u00edli de cianina 3 [equivalente al \u00e9ster Cy3\u00ae NHS] a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>120.623 \u00b5L<\/td>603.114 \u00b5L<\/td>1.206 mL<\/td>6.031 mL<\/td>12.062 mL<\/td><\/tr>
5 mM<\/strong><\/td>24.125 \u00b5L<\/td>120.623 \u00b5L<\/td>241.246 \u00b5L<\/td>1.206 mL<\/td>2.412 mL<\/td><\/tr>
10 mM<\/strong><\/td>12.062 \u00b5L<\/td>60.311 \u00b5L<\/td>120.623 \u00b5L<\/td>603.114 \u00b5L<\/td>1.206 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Las c\u00e9lulas HeLa se incubaron con (Tubulina+) o sin (Tubulina-) anti-tubulina de rat\u00f3n seguido de conjugado de IgG anti-rat\u00f3n de cabra Cy3\u00ae de AAT (Rojo, Izquierda) o IgG anti-rat\u00f3n de cabra de Jackson conjugado con Cy3\u00ae (Rojo , Derecha), respectivamente. Los n\u00facleos celulares se ti\u00f1eron con Hoechst 33342 (Blue, Cat# 17530).<\/p>\n\n\n\n

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Figura 2.<\/strong> Estructura qu\u00edmica del \u00e9ster monosuccinimid\u00edlico de cianina 3 [equivalente al \u00e9ster Cy3\u00ae NHS]<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td>Correction Factor (482 nm)<\/td>Correction Factor (565 nm)<\/td><\/tr>
Cyanine 3 bissuccinimidyl ester [equivalent to Cy3\u00ae bisNHS ester]<\/a><\/td>555<\/td>569<\/td>1500001<\/sup><\/td>0.151<\/sup><\/td>0.07<\/td>0.073<\/td>–<\/td>–<\/td><\/tr>
Cyanine 5 monosuccinimidyl ester [equivalent to Cy5\u00ae NHS ester]<\/a><\/td>651<\/td>670<\/td>2500001<\/sup><\/td>0.271<\/sup>, 0.42<\/sup><\/td>0.02<\/td>0.03<\/td>0.009<\/td>0.09<\/td><\/tr>
Cyanine 7 monosuccinimidyl ester [equivalent to Cy7\u00ae NHS ester]<\/a><\/td>756<\/td>779<\/td>250000<\/td>0.3<\/td>0.05<\/td>0.036<\/td>0.0005<\/td>0.0193<\/td><\/tr><\/tbody><\/table>
1<\/sup> PBS <\/em>2<\/sup> Ethanol<\/em><\/figcaption><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 28 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

A liquid-to-solid phase transition of Cu\/Zn superoxide dismutase 1 (SOD1) initiated by oxidation and disease mutation<\/a>
Authors: <\/strong>Gu, Siyu and Xu, Ming and Chen, Long and Shi, Xiangyan and Luo, Shi-Zhong
Journal: <\/strong>Journal of Biological Chemistry (2022): 102857<\/p>\n\n\n\n

Arc weakens synapses by dispersing AMPA receptors from postsynaptic density via modulating PSD phase separation<\/a>
Authors: <\/strong>Chen, Xudong and Jia, Bowen and Araki, Yoichi and Liu, Bian and Ye, Fei and Huganir, Richard and Zhang, Mingjie
Journal: <\/strong>Cell Research (2022): 914–930<\/p>\n\n\n\n

Caveat fluorophore: an insiders\u2019 guide to small-molecule fluorescent labels<\/a>
Authors: <\/strong>Grimm, Jonathan B and Lavis, Luke D
Journal: <\/strong>Nature methods (2022): 149–158<\/p>\n\n\n\n

Phase separation-mediated condensation of Whirlin-Myo15-Eps8 stereocilia tip complex<\/a>
Authors: <\/strong>Lin, Lin and Shi, Yingdong and Wang, Mengli and Wang, Chao and Lu, Qing and Zhu, Jinwei and Zhang, Rongguang
Journal: <\/strong>Cell Reports (2021): 108770<\/p>\n\n\n\n

A simple lectin-based biochip might display the potential clinical value of glycomics in patients with spontaneous intracerebral hemorrhage<\/a>
Authors: <\/strong>Ye, Lei and Fang, Yong-Sheng and Li, Xiao-Xue and Gao, Yi and Liu, Sheng-Sheng and Chen, Qiang and Wu, Qiang and Cheng, Hong-Wei and Du, Wei-Dong
Journal: <\/strong>Annals of Translational Medicine (2021)<\/p>\n\n\n\n

Kindlin2-mediated phase separation underlies integrin adhesion formation<\/a>
Authors: <\/strong>Li, Yujie and Zhang, Ting and Li, Huadong and Yang, Haibin and Lin, Ruihong and Sun, Kang and Wang, Lei and Zhang, Jing and Wei, Zhiyi and Yu, Cong
Journal: <\/strong>BioRxiv (2020)<\/p>\n\n\n\n

Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid mono-N-hydroxysuccinimide ester (DOTA-NHS-ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers<\/a>
Authors: <\/strong>Gao, Yi and Li, Song-Guo and Liu, Qian and Liu, Sheng-Sheng and Ye, Lei and Song, Zi-Jian and Du, Wei-Dong
Journal: <\/strong>Analytical Biochemistry (2020): 113686<\/p>\n\n\n\n

Par complex cluster formation mediated by phase separation<\/a>
Authors: <\/strong>Liu, Ziheng and Yang, Ying and Gu, Aihong and Xu, Jiawen and Mao, Ying and Lu, Haojie and Hu, Weiguo and Lei, Qun-Ying and Li, Zhouhua and Zhang, Mingjie and others,
Journal: <\/strong>Nature communications (2020): 1–18<\/p>\n\n\n\n

Phase separation-mediated formation of condensed GIT\/PIX enzyme complex module for compartmentalized signaling
Authors: <\/strong>Zhu, Jinwei and Zhou, Qingqing and Xia, Yitian and Lin, Lin and Li, Jianchao and Peng, Mengjuan and Zhang, Rongguang and Zhang, Mingjie
Journal: <\/strong>bioRxiv (2019)<\/p>\n\n\n\n

Role of flagellar hydrogen bonding in Salmonella motility and flagellar polymorphic transition<\/a>
Authors: <\/strong>Wang, Chu and Lunelli, Michele and Zschieschang, Erik and Bosse, Jens and Thuenauer, Rol and , undefined and Kolbe, Michael
Journal: <\/strong>Molecular microbiology (2019)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ve todas las 21 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: <\/strong>Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: <\/strong>Appl Phys Lett (2011): 143703<\/p>\n\n\n\n

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: <\/strong>Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: <\/strong>Theranostics (2011): 381<\/p>\n\n\n\n

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: <\/strong>Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: <\/strong>Biochemistry (2011): 2691<\/p>\n\n\n\n

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: <\/strong>Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: <\/strong>JACC Cardiovasc Imaging (2009): 1114<\/p>\n\n\n\n

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: <\/strong>Volke D, Hoffmann R.
Journal: <\/strong>Electrophoresis (2008): 4516<\/p>\n\n\n\n

Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
Authors: <\/strong>Vareiro MM, Tranchant I, Maplin S, Zak K, Gani MM, Slevin CJ, Hailes HC, Tabor AB, Cameron PJ, Jenkins AT, Williams DE.
Journal: <\/strong>Anal Biochem (2008): 243<\/p>\n\n\n\n

Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer
Authors: <\/strong>Menacher F, Rubner M, Berndl S, Wagenknecht HA.
Journal: <\/strong>J Org Chem (2008): 4263<\/p>\n\n\n\n

Near-infrared fluorescence imaging of tumor integrin alpha v beta 3 expression with Cy7-labeled RGD multimers
Authors: <\/strong>Wu Y, Cai W, Chen X.
Journal: <\/strong>Mol Imaging Biol (2006): 226<\/p>\n\n\n\n

Cy7-Bis-dipicolylamine-zinc
Authors: <\/strong>Leung K., undefined
Journal: <\/strong>In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)<\/p>\n\n\n\n

Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: <\/strong>Cheng KT., undefined
Journal: <\/strong>In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)<\/p>\n\n\n\n

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Application Notes (en Ingles) <\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Equivalente a Cy3\u00ae NHS ester<\/p>\n","protected":false},"featured_media":2218,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2151"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":64,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2151\/revisions"}],"predecessor-version":[{"id":3702,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/2151\/revisions\/3702"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2218"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=2151"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=2151"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}