{"id":12021,"date":"2023-05-02T17:19:39","date_gmt":"2023-05-02T22:19:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=12021"},"modified":"2023-05-02T17:39:43","modified_gmt":"2023-05-02T22:39:43","slug":"indicador-ing-2-am","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/indicador-ing-2-am\/","title":{"rendered":"Indicador ING-2 AM"},"content":{"rendered":"\n

ION Natrium Green \u2013 2 (ING-2) es un indicador de sodio intracelular (Na\u207a) fluorescente de color amarillo verdoso con una carga celular mejorada y un brillo significativamente mayor que el SBFI. Sus propiedades espectrales (Ex\/Em: 525 nm\/545 nm) y su gran rango din\u00e1mico hacen de ING-2 el mejor indicador de Na\u207a para aplicaciones de detecci\u00f3n de alto rendimiento dirigidas a canales de Na\u207a y canales de cationes monovalentes no selectivos. ING-2 tiene una afinidad fisiol\u00f3gicamente m\u00e1s relevante (Kd = 20 mM) que ING-1 (Kd = 92 mM). Tambi\u00e9n es compatible con la microscop\u00eda de fluorescencia utilizando filtros comunes de fluoresce\u00edna, GFP o, mejor a\u00fan, YFP.<\/p>\n\n\n\n

Sin\u00f3nimos:<\/strong>\u00a0ION Natrium Green \u2013 2, Asante Natrium Green \u2013 2 (ANG-2)<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
ON-2011F<\/td>Indicador ING-2 AM<\/td>10 x 50 ug<\/td><\/tr>
ON-2011<\/td>Indicador ING-2 AM<\/td>3 x 50 ug<\/td><\/tr>
ON-2011C<\/td>Indicador ING-2 AM<\/td>500 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Especificaciones<\/a><\/p>\n\n\n\n

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SDS<\/a><\/p>\n\n\n\n

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Protocolo<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n

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Especificaciones<\/strong><\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>525 nm\u00a0<\/a><\/td><\/tr>
Emisi\u00f3n<\/td>545 nm<\/a><\/td><\/tr>
Kd:<\/td>20 mM<\/td><\/tr>
Solubilidad<\/td>DMSO<\/td><\/tr>
Peso molecular<\/td>1084 g\/mol<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Este es el principal indicador de Na\u207a. El ING-2 es un indicador an\u00e1logo de mayor afinidad de ING-1. Con id\u00e9nticas longitudes de onda de excitaci\u00f3n y emisi\u00f3n. Conserva las propiedades deseables de f\u00e1cil carga, retenci\u00f3n celular y buena fotoestabilidad.<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Laila Bl\u00f6mer, Marco Canepari, Luiza Filipis. Ultrafast Sodium Imaging of the Axon Initial Segment of Neurons in Mouse Brain Slices. Current Protocols in Bioinformatics<\/em>, Wiley, 2021, 1 (3).<\/a> https:\/\/doi.org\/10.1002\/cpz1.64<\/a><\/p>\n\n\n\n

NAUMANN, G., LIPPMANN, K. and EILERS, J. (2018), Photophysical properties of Na+\u2010indicator dyes suitable for quantitative two\u2010photon fluorescence\u2010lifetime measurements. Journal of Microscopy, 272: 136-144. <\/a><\/p>\n\n\n\n

Tay B, Stewart TA, Davis FM, Deuis JR, Vetter I (2019) Development of a high-throughput fluorescent no-wash sodium influx assay. PLOS ONE 14(3): e0213751. https:\/\/doi.org\/10.1371\/journal.pone.0213751<\/a><\/p>\n\n\n\n

Yurinskaya VE, Aksenov ND, Moshkov AV, Goryachaya TS, Vereninov AA. Fluorometric Na+ Evaluation in Single Cells Using Flow Cytometry: Comparison with Flame Emission Assay. Cell Physiol Biochem. 2020 May 29;54(4):556-566. doi: 10.33594\/000000239.<\/a><\/p>\n\n\n\n

Aliotta, A., Bertaggia Calderara, D. and Alberio, L. (2020), Flow Cytometric Monitoring of Dynamic Cytosolic Calcium, Sodium, and Potassium Fluxes Following Platelet Activation. Cytometry, 97: 933-944. https:\/\/doi.org\/10.1002\/cyto.a.24017<\/a><\/p>\n\n\n\n

Yao L, Fan P, Jiang Z, Viatchenko-Karpinski S, Wu Y, Kornyeyev D, Hirakawa R, Budas GR, Rajamani S, Shryock JC, Belardinelli L. Nav1.5-dependent persistent Na+ influx activates CaMKII in rat ventricular myocytes and N1325S mice. Am J Physiol Cell Physiol. 2011 Sep;301(3):C577-86. doi: 10.1152\/ajpcell.00125.2011<\/a><\/p>\n\n\n\n

Iamshanova, O., Mariot, P., Lehen\u2019kyi, V. et al. Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines. Eur Biophys J 45, 765\u2013777 (2016). https:\/\/doi.org\/10.1007\/s00249-016-1173-7<\/a><\/p>\n\n\n\n

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Garantia ON Assurance. La calidad de este producto esta garantizado. Si no est\u00e1 satisfecho con su pedido, trabajaremos para resolver de inmediato su inquietud de acuerdo con nuestros t\u00e9rminos y condiciones de ION Assurance.<\/p>\n\n\n\n

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ING-2 AM es un indicador de sodio fluorescente amarillo-verde. Permeable a la membrana. Mayor afinidad por el sodio que ING-1. Adecuado para el cribado de alto rendimiento de canales y transportadores de sodio.<\/p>\n","protected":false},"featured_media":12046,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/12021"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/12021\/revisions"}],"predecessor-version":[{"id":12048,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/12021\/revisions\/12048"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/12046"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=12021"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=12021"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}