{"id":11012,"date":"2023-04-18T12:34:10","date_gmt":"2023-04-18T17:34:10","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=11012"},"modified":"2023-04-18T13:08:33","modified_gmt":"2023-04-18T18:08:33","slug":"trypan-ultrablue-sal-de-sodio-grado-ultrapuro","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/trypan-ultrablue-sal-de-sodio-grado-ultrapuro\/","title":{"rendered":"Trypan Blue, sal de sodio, Grado UltraPuro\u00a0"},"content":{"rendered":"\n

El azul trip\u00e1n, un colorante diazoico, es una tinci\u00f3n vital que ti\u00f1e de azul los tejidos muertos o las c\u00e9lulas. Las c\u00e9lulas vivas o los tejidos con membranas celulares intactas no se colorean. Dado que las c\u00e9lulas son muy selectivas en los compuestos que atraviesan la membrana, en una c\u00e9lula viable el azul de tripano no se absorbe; sin embargo, atraviesa la membrana en una c\u00e9lula muerta. Por lo tanto, las c\u00e9lulas muertas se muestran como un color azul distintivo bajo un microscopio. <\/p>\n\n\n\n

El azul de tripano se usa com\u00fanmente en microscop\u00eda (para el recuento de c\u00e9lulas) y en ratones de laboratorio para evaluar la viabilidad de los tejidos. El m\u00e9todo no puede distinguir entre c\u00e9lulas necr\u00f3ticas y apopt\u00f3ticas. Tambi\u00e9n es \u00fatil observar hifas de hongos y Stramenopiles. Ofrecemos la m\u00e1s alta pureza de Trypan Blue para eliminar la impurezas fluorescentes. <\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-2452<\/td>Trypan Blue, sal de sodio, UltraPure grade<\/td>10 g<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). No est\u00e1 dise\u00f1ado para uso diagn\u00f3stico o terap\u00e9utico. Almacenamiento a temperatura ambiente 10 a 25\u00b0C. Minimizar la exposici\u00f3n a la luz.
La fecha de caducidad es de 6 meses a partir de la fecha de recepci\u00f3n.<\/p>\n\n\n\n

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Propiedades Fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>960.81<\/td><\/tr>
Disolvente<\/td>Agua<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark>
Volumen de agua necesario para reconstituir la masa espec\u00edfica de Trypan Blue, sal de sodio UltraPure Grade a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>104.079 \u00b5L<\/td>520.394 \u00b5L<\/td>1.041 mL<\/td>5.204 mL<\/td>10.408 mL<\/td><\/tr>
5 mM<\/strong><\/td>20.816 \u00b5L<\/td>104.079 \u00b5L<\/td>208.158 \u00b5L<\/td>1.041 mL<\/td>2.082 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.408 \u00b5L<\/td>52.039 \u00b5L<\/td>104.079 \u00b5L<\/td>520.394 \u00b5L<\/td>1.041 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Figura 1. <\/strong>Estructura quimica para Trypan Blue, sal de sodio Grado UltraPuro<\/p>\n\n\n\n

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Formatos Alternativos<\/mark><\/p>\n\n\n\n

Trypan Blue, sodium salt *CAS 72-57-1*<\/a><\/td><\/tr>
Trypan Blue, sodium salt *10 mM aqueous solution*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 106 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: <\/strong>Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: <\/strong>J Cataract Refract Surg (2006): 1483<\/p>\n\n\n\n

A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: <\/strong>Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: <\/strong>Eye (2006): 820<\/p>\n\n\n\n

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: <\/strong>Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: <\/strong>Am J Ophthalmol (2006): 754<\/p>\n\n\n\n

Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: <\/strong>Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: <\/strong>Am J Ophthalmol (2005): 132<\/p>\n\n\n\n

Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: <\/strong>Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: <\/strong>Quintessence Int (2005): 702<\/p>\n\n\n\n

Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: <\/strong>Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: <\/strong>J Cataract Refract Surg (2005): 938<\/p>\n\n\n\n

Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: <\/strong>Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: <\/strong>Exp Eye Res (2005): 395<\/p>\n\n\n\n

Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: <\/strong>Healey PR, Crowston JG.
Journal: <\/strong>Br J Ophthalmol (2005): 1152<\/p>\n\n\n\n

Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: <\/strong>Yamamoto N, Ozaki N, Murakami K.
Journal: <\/strong>Ophthalmologica (2005): 338<\/p>\n\n\n\n

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: <\/strong>Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: <\/strong>Transfusion (2005): 1208<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Calcein<\/a>
Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates<\/a>
Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes<\/a><\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

Are inflammasomes and caspase-1 related?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there upgraded trypan blue derivatives for cell viability testing?<\/a>
Can 7-AAD be fixed?<\/a>
Can 7-AAD stain live cells?<\/a><\/p>\n\n\n\n

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AssayWise <\/mark><\/p>\n\n\n\n

Nucleic Acid Detection, Quantification and Imaging<\/a>
A practical guide for use of PE and APC in flow cytometry<\/a>
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin Filaments<\/a>
Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL<\/a>
Power Styramide\u2122 Signal Amplification a Superior Alternative to Tyramide Signal Amplification<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Colorante purificado para eliminar las impurezas fluorescentes<\/p>\n","protected":false},"featured_media":11013,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11012"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11012\/revisions"}],"predecessor-version":[{"id":11018,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11012\/revisions\/11018"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/11013"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=11012"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=11012"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}