{"id":11001,"date":"2023-04-17T20:04:21","date_gmt":"2023-04-18T01:04:21","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=11001"},"modified":"2023-04-17T20:09:07","modified_gmt":"2023-04-18T01:09:07","slug":"trypan-purple","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/trypan-purple\/","title":{"rendered":"Trypan Purple\u2122"},"content":{"rendered":"\n<p>Trypan Purple\u2122 es similar al Trypan Blue en permeabilidad celular. No es permeable a las c\u00e9lulas vivas. En comparaci\u00f3n con Trypan Blue, Trypan Purple\u2122 es menos t\u00f3xico para las c\u00e9lulas, en particular, tiene un efecto m\u00ednimo sobre los receptores de la superficie celular, como los receptores acoplados a prote\u00edna G (GPCR). Otra ventaja es que las c\u00e9lulas se pueden observar claramente bajo el microscopio cuando se usa Trypan Purple\u2122 mientras que Trypan Blue hace que sea bastante dif\u00edcil ver las c\u00e9lulas bajo el microscopio. <\/p>\n\n\n\n<p>Nuestro Trypan Purple\u2122 tambi\u00e9n se puede usar para prevenir tintes fluorescentes (como FDA, rodamina 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calce\u00edna AM, Fluo-3 AM, Fluo-4 AM , Quest Fluo-8\u2122 AM y Rhod-4\u2122 AM) se escapen de las celdas. Podr\u00eda inhibir las actividades de las bombas de salida de f\u00e1rmacos, ya que contiene un resto similar al probenecid, como se muestra a continuaci\u00f3n. En comparaci\u00f3n con el probenecid, es neutro, altamente soluble en agua y c\u00f3modo de usar. Su mecanismo celular a\u00fan est\u00e1 bajo investigaci\u00f3n. <\/p>\n\n\n\n<p>El Trypan Purple\u2122 est\u00e1 altamente purificado y puede usarse hasta 0.75 mM con una citotoxicidad celular m\u00ednima. Nuestra concentraci\u00f3n recomendada es de 0.5 mM.<\/p>\n\n\n\n<div style=\"height:26px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>AAT-2465<\/td><td>Trypan Purple\u2122, 0.1 M aqueous solution<\/td><td>10 ml<\/td><\/tr><tr><td>AAT-2466<\/td><td>Trypan Purple\u2122, 0.1 M aqueous solution<\/td><td>100 ml<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:12px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-1 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/safety-data-sheet-sds\/safety-data-sheet-for-trypan-purple-0-1-m-aqueous-solution-catalog-2465-version-CHIvgpzW0a.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">SDS<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/docs.aatbio.com\/products\/protocol-and-product-information-sheet-pis\/protocol-for-trypan-purple-0-1-m-aqueous-solution-version-946a6b3cec.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Protocol<\/a><\/p>\n<\/div>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Importante, Solo para uso en investigaci\u00f3n (RUO). No est\u00e1 dise\u00f1ado para uso diagn\u00f3stico o terap\u00e9utico. Almacenamiento: Refrigeraci\u00f3n 2-8 \u00b0C. Minimizar la exposici\u00f3n a la luz.<br>La fecha de caducidad es de 6 meses a partir de la fecha de recepci\u00f3n.<\/p>\n\n\n\n<div style=\"height:56px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Propiedades Fisicas<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Peso Molecular <\/td><td>~600<\/td><\/tr><tr><td>Disolvente<\/td><td>Agua<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:89px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" width=\"328\" height=\"164\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-16.png\" alt=\"\" class=\"wp-image-10995\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-16.png 328w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-16-300x150.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-16-280x140.png 280w\" sizes=\"(max-width: 328px) 100vw, 328px\" \/><figcaption><strong>Figura&nbsp;1.&nbsp;<\/strong>La estructura de Trypan Red Plus\u2122 (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).<\/figcaption><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Bibliograf\u00eda<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\">Comparative Analyses of Murine and Human Formyl Peptide Receptor 3<br><strong>Authors:&nbsp;<\/strong>Stempel, Hendrik<br><strong>Journal:&nbsp;<\/strong>(2017)<\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"http:\/\/www.jbc.org\/content\/291\/18\/9762.short\" target=\"_blank\" rel=\"noreferrer noopener\">Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems<\/a><br><strong>Authors:&nbsp;<\/strong>Stempel, Hendrik and Jung, Martin and P&amp;eacute;rez-G&amp;oacute;mez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd<br><strong>Journal:&nbsp;<\/strong>Journal of Biological Chemistry&nbsp;(2016):&nbsp;9762&#8211;9775<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Referencias<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><em>Ver todas las 32 references:&nbsp;<a href=\"https:\/\/www.aatbio.com\/resources\/citation-explorer?catalog=2457\">Citation Explorer<\/a><\/em><\/p>\n\n\n\n<p style=\"font-size:14px\">Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells<br><strong>Authors:&nbsp;<\/strong>Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.<br><strong>Journal:&nbsp;<\/strong>Retina&nbsp;(2007):&nbsp;375<\/p>\n\n\n\n<p style=\"font-size:14px\">Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract<br><strong>Authors:&nbsp;<\/strong>Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.<br><strong>Journal:&nbsp;<\/strong>J Cataract Refract Surg&nbsp;(2006):&nbsp;1483<\/p>\n\n\n\n<p style=\"font-size:14px\">Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin<br><strong>Authors:&nbsp;<\/strong>Li Q, Kato Y, Sai Y, Imai T, Tsuji A.<br><strong>Journal:&nbsp;<\/strong>Pharm Res&nbsp;(2005):&nbsp;842<\/p>\n\n\n\n<p style=\"font-size:14px\">Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy<br><strong>Authors:&nbsp;<\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.<br><strong>Journal:&nbsp;<\/strong>Gen Physiol Biophys&nbsp;(2004):&nbsp;357<\/p>\n\n\n\n<p style=\"font-size:14px\">A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor<br><strong>Authors:&nbsp;<\/strong>Heinemann A, Ofner M, Amann R, Peskar BA.<br><strong>Journal:&nbsp;<\/strong>Pharmacology&nbsp;(2003):&nbsp;49<\/p>\n\n\n\n<p style=\"font-size:14px\">Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A<br><strong>Authors:&nbsp;<\/strong>Abrahamse SL, Rechkemmer G.<br><strong>Journal:&nbsp;<\/strong>Pflugers Arch&nbsp;(2001):&nbsp;529<\/p>\n\n\n\n<p style=\"font-size:14px\">Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures<br><strong>Authors:&nbsp;<\/strong>Fast VG, Ideker RE.<br><strong>Journal:&nbsp;<\/strong>J Cardiovasc Electrophysiol&nbsp;(2000):&nbsp;547<\/p>\n\n\n\n<p style=\"font-size:14px\">Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor<br><strong>Authors:&nbsp;<\/strong>Kermode JC, Zheng Q, Cook EP.<br><strong>Journal:&nbsp;<\/strong>Blood Cells Mol Dis&nbsp;(1996):&nbsp;238<\/p>\n\n\n\n<p style=\"font-size:14px\">Probenecid inhibits platelet responses to aggregating agents in vitro and has a synergistic inhibitory effect with penicillin G<br><strong>Authors:&nbsp;<\/strong>Packham MA, R and ML, Perry DW, Ruben DH, Kinlough-Rathbone RL.<br><strong>Journal:&nbsp;<\/strong>Thromb Haemost&nbsp;(1996):&nbsp;239<\/p>\n\n\n\n<p style=\"font-size:14px\">Quantitative analysis of phagocytosis and killing of Cryptococcus neoformans by human peripheral blood mononuclear cells by flow cytometry<br><strong>Authors:&nbsp;<\/strong>Chaka W, Scharringa J, Verheul AF, Verhoef J, Van Strijp AG, Hoepelman IM.<br><strong>Journal:&nbsp;<\/strong>Clin Diagn Lab Immunol&nbsp;(1995):&nbsp;753<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Application notes <\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/abbreviation-of-common-chemical-compounds-related-to-peptides\" target=\"_blank\" rel=\"noopener\" title=\"\">Abbreviation of Common Chemical Compounds Related to Peptides<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/annexin-v\" target=\"_blank\" rel=\"noopener\" title=\"\">Annexin V<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/calcein\" target=\"_blank\" rel=\"noopener\" title=\"\">Calcein<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/human-high-temperature-requirement-serine-protease-a1-htra1-degrades-tau-protein-aggregates\" target=\"_blank\" rel=\"noopener\" title=\"\">Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/application-notes\/multi-color-labeling-and-functional-analysis-of-live-cells-using-fluorescent-calcein-am-dyes\" target=\"_blank\" rel=\"noopener\" title=\"\">Multi-Color Labeling and Functional Analysis of Live Cells Using Fluorescent Calcein AM Dyes<\/a><\/p>\n\n\n\n<div style=\"height:46px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">FAQ<\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.aatbio.com\/resources\/faq-frequently-asked-questions\/Are-inflammasomes-and-caspase-1-related\" target=\"_blank\" rel=\"noopener\" title=\"\">Are inflammasomes and caspase-1 related?<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/faq-frequently-asked-questions\/Are-there-any-alternatives-to-BrdU-Bromodeoxyuridine\" target=\"_blank\" rel=\"noopener\" title=\"\">Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/faq-frequently-asked-questions\/Are-there-upgraded-trypan-blue-derivatives-for-cell-viability-testing\" target=\"_blank\" rel=\"noopener\" title=\"\">Are there upgraded trypan blue derivatives for cell viability testing?<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/faq-frequently-asked-questions\/can-7-aad-be-fixed\" target=\"_blank\" rel=\"noopener\" title=\"\">Can 7-AAD be fixed?<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/faq-frequently-asked-questions\/Can-7-AAD-stain-live-cells\" target=\"_blank\" rel=\"noopener\" title=\"\">Can 7-AAD stain live cells?<\/a><\/p>\n\n\n\n<div style=\"height:68px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">AssayWise <\/mark><\/p>\n\n\n\n<p style=\"font-size:14px\"><a href=\"https:\/\/www.aatbio.com\/resources\/assaywise\/2017-6-1\/nucleic-acid-detection-quantification-and-imaging\" target=\"_blank\" rel=\"noopener\" title=\"\">Nucleic Acid Detection, Quantification and Imaging<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/assaywise\/2017-6-2\/a-practical-guide-for-use-of-pe-and-apc-in-flow-cytometry\" target=\"_blank\" rel=\"noopener\" title=\"\">A practical guide for use of PE and APC in flow cytometry<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/assaywise\/2018-7-1\/fluorescent-phalloidin-a-practical-stain-for-visualizing-actin-filaments\" target=\"_blank\" rel=\"noopener\" title=\"\">Fluorescent Phalloidin: A Practical Stain for Visualizing Actin Filaments<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/assaywise\/2018-7-2\/intracellular-ph-measurement-with-dual-excitation-fluorescence-sensor-bcfl\" target=\"_blank\" rel=\"noopener\" title=\"\">Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL<\/a><br><a href=\"https:\/\/www.aatbio.com\/resources\/assaywise\/2019-8-2\/power-styramide-signal-amplification-a-superior-alternative-to-tyramide-signal-amplification\" target=\"_blank\" rel=\"noopener\" title=\"\">Power Styramide\u2122 Signal Amplification a Superior Alternative to Tyramide Signal Amplification<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Colorante en soluci\u00f3n acuosa de 0.1 M <\/p>\n","protected":false},"featured_media":10995,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11001"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11001\/revisions"}],"predecessor-version":[{"id":11004,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/11001\/revisions\/11004"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/10995"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=11001"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=11001"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}