{"id":10255,"date":"2023-04-04T23:25:39","date_gmt":"2023-04-05T04:25:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=10255"},"modified":"2023-04-11T12:59:29","modified_gmt":"2023-04-11T17:59:29","slug":"purecube-co-nta-agarose-xl","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/purecube-co-nta-agarose-xl\/","title":{"rendered":"PureCube Co-NTA Agarose XL"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"10255\" class=\"elementor elementor-10255\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-70a4f1cc elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"70a4f1cc\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6e2b779a\" data-id=\"6e2b779a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4e9b82f0 elementor-widget elementor-widget-text-editor\" data-id=\"4e9b82f0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p><!-- wp:spacer {\"height\":\"12px\"} --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Nuestras resinas de agarosa PureCube Co-NTA XL son perlas de resina de agarosa con un di\u00e1metro de ~400 \u00b5m. Se utilizan para la purificaci\u00f3n de prote\u00ednas etiquetadas con His activas de las c\u00e9lulas.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Nuestra experiencia de un a\u00f1o en la fabricaci\u00f3n de resina de agarosa condujo al alto rendimiento de 30 mg de prote\u00edna por ml de resina, que es l\u00edder en el mercado en comparaci\u00f3n con otros proveedores de Co-NTA. Las resinas PureCube Co-NTA son adecuadas para columnas de centrifugaci\u00f3n por lotes y FPLC. Su gran di\u00e1metro proporciona grandes velocidades de flujo incluso para los medios celulares m\u00e1s agresivos.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Para mayores rendimientos de prote\u00edna y estabilidad mec\u00e1nica, recomendamos nuestras perlas de <a title=\"\" href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/his-tag-affinity-resins-magbeads\/co-nta\/purecube-co-nta-agarose\/31403\" target=\"_blank\" rel=\"noopener\">agarosa Co-NTA con un di\u00e1metro de 40 \u00b5m<\/a> y su equivalente de <a title=\"\" href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/his-tag-affinity-resins-magbeads\/co-nta\/purecube-100-co-nta-agarose\/74403\" target=\"_blank\" rel=\"noopener\">100 \u00b5m<\/a>.<br \/>Adem\u00e1s, tambi\u00e9n puede obtener nuestras perlas Co-NTA como <a title=\"\" href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/his-tag-affinity-resins-magbeads\/co-nta\/purecube-co-nta-magbeads\/31505\" target=\"_blank\" rel=\"noopener\">perlas magn\u00e9ticas<\/a>.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:spacer {\"height\":\"80px\"} --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:table {\"style\":{\"color\":{\"gradient\":\"linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"},\"typography\":{\"fontSize\":\"18px\"}},\"textColor\":\"black\"} --><\/p>\n<figure class=\"wp-block-table\" style=\"font-size: 18px;\">\n<table class=\"has-black-color has-text-color has-background\" style=\"background: linear-gradient(0deg,#eeeeee 0%,#ffffff 28%,#f9f9f9 72%,#a9b8c3 100%);\">\n<thead>\n<tr>\n<th>Catalogo<\/th>\n<th>Producto<\/th>\n<th>Presentaci\u00f3n<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>CU-55403<\/td>\n<td>PureCube Co-NTA Agarose XL<\/td>\n<td>10 ml<\/td>\n<\/tr>\n<tr>\n<td>CU-55405<\/td>\n<td>PureCube Co-NTA Agarose XL<\/td>\n<td>50 ml<\/td>\n<\/tr>\n<tr>\n<td>CU-55410<\/td>\n<td>PureCube Co-NTA Agarose XL<\/td>\n<td>250 ml<\/td>\n<\/tr>\n<tr>\n<td>CU-55412<\/td>\n<td>PureCube Co-NTA Agarose XL<\/td>\n<td>500 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<p><!-- \/wp:table --><\/p>\n<p><!-- wp:spacer {\"height\":\"36px\"} --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:group {\"layout\":{\"type\":\"flex\",\"flexWrap\":\"nowrap\"}} --><\/p>\n<div class=\"wp-block-group\">\n<p><!-- wp:image {\"id\":9682,\"sizeSlug\":\"full\",\"linkDestination\":\"none\"} --><\/p>\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" width=\"32\" height=\"32\" class=\"wp-image-9682\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-215.png\" alt=\"\" \/><\/figure>\n<p><!-- \/wp:image --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><mark class=\"has-inline-color has-vivid-cyan-blue-color\" style=\"background-color: rgba(0, 0, 0, 0);\">Hojas de Datos \/ Protocolos<\/mark><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<\/div>\n<p><!-- \/wp:group --><\/p>\n<p><!-- wp:spacer {\"height\":\"21px\"} --><\/p>\n<div class=\"wp-block-spacer\" style=\"height: 21px;\" aria-hidden=\"true\">\u00a0<\/div>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:list {\"style\":{\"typography\":{\"fontSize\":\"16px\"}}} --><\/p>\n<ul style=\"font-size: 16px;\">\n<li><a title=\"\" href=\"https:\/\/cube-biotech.com\/media\/bd\/c7\/f6\/1666342538\/55403%20Co-NTA%20XL%20Agarose.pdf\" target=\"_blank\" rel=\"noopener\">Co-NTA XL agarose\u00a0Datasheet<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/fb\/ba\/27\/1664969680\/His-Agarose%20Native.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Native Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/e6\/1d\/75\/1664969680\/His-Agarose%20Denaturing.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Denaturing Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/e4\/09\/2b\/1664969680\/His-Batch-Spin%20Mini%20Native.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Batchspin MINI Native Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/18\/84\/47\/1664969680\/His-Batch-Spin%20Mini%20Denaturing.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Batchspin MINI Denaturing Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/70\/bb\/be\/1664969680\/His-Batch-Spin%20MIDI%20Native.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Batchspin MIDI Native Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/15\/2f\/45\/1664969680\/His-Batch-Spin%20MIDI%20Denaturing.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Batchspin MIDI Denaturing Purification\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/09\/7f\/2d\/1664969698\/His-Washing%20Regeneration%20NTA%20IDA.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Washing &amp; Regeneration\u00a0Protocol<\/a><\/li>\n<li><a href=\"https:\/\/cube-biotech.com\/media\/13\/29\/6f\/1664969698\/Packing%20Cartridge%20Columns.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Cartridge Packing\u00a0Protocol<\/a><\/li>\n<\/ul>\n<p><!-- \/wp:list --><\/p>\n<p><!-- wp:spacer {\"height\":\"85px\"} --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>\u00a0<\/p>\n<p><mark class=\"has-inline-color has-vivid-cyan-blue-color\" style=\"background-color: rgba(0, 0, 0, 0);\">Caracter\u00edsticas<\/mark><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:table --><\/p>\n<figure class=\"wp-block-table\">\n<table>\n<tbody>\n<tr>\n<td>Usage<\/td>\n<td>Specific binding and purification of 6x His tagged proteins<\/td>\n<\/tr>\n<tr>\n<td>Specificity<\/td>\n<td>Affinity to His tagged proteins<\/td>\n<\/tr>\n<tr>\n<td>Binding capacity<\/td>\n<td>&gt;30\u202fmg\/mL<\/td>\n<\/tr>\n<tr>\n<td>Bead Ligand<\/td>\n<td>Co-NTA<\/td>\n<\/tr>\n<tr>\n<td>Bead size<\/td>\n<td>400 \u03bcm<\/td>\n<\/tr>\n<tr>\n<td>Filling quantity<\/td>\n<td>Delivered as a 50\u202f% suspension<\/td>\n<\/tr>\n<tr>\n<td>Required equipment<\/td>\n<td>Lysis Buffer<br \/>Wash Buffer<br \/>Elution Buffer<br \/>Ice bath<br \/>Refrigerated centrifuge for 50\u202fmL tube (min 10,000 x g)<br \/>50\u202fmL centrifuge tube<br \/>Micropipettor and Micropipetting tips<br \/>Disposable gravity flow columns with capped bottom outlet, 2 ml<br \/>pH meter<br \/>End-over-end shaker<br \/>SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n<p><!-- \/wp:table --><\/p>\n<p><!-- wp:spacer --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><mark class=\"has-inline-color has-vivid-cyan-blue-color\" style=\"background-color: rgba(0, 0, 0, 0);\">Envio y Almacenamiento<\/mark><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph {\"style\":{\"typography\":{\"fontSize\":\"16px\"}}} --><\/p>\n<p style=\"font-size: 16px;\">Este producto se envia a temperatura ambiente. Almacenamiento de corto plazo: En buffer neutral. Almacenamiento a largo plazo: En buffer neutral con 20% etanol a 4\u00b0C.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:spacer --><\/p>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><mark class=\"has-inline-color has-vivid-cyan-blue-color\" style=\"background-color: rgba(0, 0, 0, 0);\">Resultado de laboratorio<\/mark><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:columns --><\/p>\n<div class=\"wp-block-columns\">\n<p><!-- wp:column --><\/p>\n<div class=\"wp-block-column\">\n<p><!-- wp:paragraph --><\/p>\n<p>Diferentes iones met\u00e1licos confieren diferente afinidad y especificidad de uni\u00f3n.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>La carga de diferentes iones met\u00e1licos en una resina da como resultado diferentes afinidades y especificidades para una prote\u00edna etiquetada con His. En general, el cobalto exhibe la mayor especificidad de uni\u00f3n de los iones met\u00e1licos IMAC com\u00fanmente utilizados, lo que conduce a rendimientos relativamente bajos pero de alta pureza. El cobre, en el otro extremo del espectro, tiene una alta afinidad que conduce a altos rendimientos pero a una uni\u00f3n inespec\u00edfica. En la b\u00fasqueda de la resina \u00f3ptima para purificar una prote\u00edna, se recomienda explorar diferentes ligandos quelantes (IDA o NTA) y diferentes iones met\u00e1licos.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<\/div>\n<p><!-- \/wp:column --><\/p>\n<p><!-- wp:column --><\/p>\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" class=\"wp-image-10064\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-1024x669.png\" alt=\"\" width=\"442\" height=\"288\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-1024x669.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-300x196.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-768x502.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-600x392.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9-245x160.png 245w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/image-9.png 1294w\" sizes=\"(max-width: 442px) 100vw, 442px\" \/><\/figure>\n<p style=\"font-size: 14px;\">Figura 1: Afinidad y especificidad de los iones met\u00e1licos com\u00fanmente utilizados para IMAC. Cargar una resina IMAC con diferentes iones met\u00e1licos puede ajustar la afinidad y la especificidad para optimizar la pureza y el rendimiento de una prote\u00edna purificada.<\/p>\n<\/div>\n<p>\u00a0<\/p>\n<p><span style=\"background-color: var( --e-global-color-3d761f9 ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-size: var( --e-global-typography-text-font-size ); font-style: var( --e-global-typography-text-font-style ); font-weight: var( --e-global-typography-text-font-weight ); letter-spacing: var( --e-global-typography-text-letter-spacing ); text-transform: var( --e-global-typography-text-text-transform );\">\u00a0<\/span><\/p>\n<\/div>\n<p><!-- \/wp:spacer --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>\u00a0<\/p>\n<p><!-- \/wp:spacer --><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e9a0952 elementor-widget elementor-widget-text-editor\" data-id=\"e9a0952\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>V\u00eddeos<\/strong><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7c5323a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7c5323a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-b11ee83\" data-id=\"b11ee83\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-11ca88e elementor-aspect-ratio-169 elementor-widget elementor-widget-video\" data-id=\"11ca88e\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/h6hAJq7ljgs&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;,&quot;aspect_ratio&quot;:&quot;169&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-video .elementor-widget-container{overflow:hidden;-webkit-transform:translateZ(0);transform:translateZ(0)}.elementor-widget-video .elementor-open-inline .elementor-custom-embed-image-overlay{position:absolute;top:0;left:0;width:100%;height:100%;background-size:cover;background-position:50%}.elementor-widget-video .elementor-custom-embed-image-overlay{cursor:pointer;text-align:center}.elementor-widget-video .elementor-custom-embed-image-overlay:hover .elementor-custom-embed-play i{opacity:1}.elementor-widget-video .elementor-custom-embed-image-overlay img{display:block;width:100%}.elementor-widget-video .e-hosted-video .elementor-video{-o-object-fit:cover;object-fit:cover}.e-container>.elementor-widget-video{width:var(--container-widget-width,100%)}<\/style>\t\t<div class=\"elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b89eead elementor-widget elementor-widget-text-editor\" data-id=\"b89eead\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>How to pack FPLC cartridges<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f7e2f1c\" data-id=\"f7e2f1c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f7ae8d5\" data-id=\"f7ae8d5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-44842af elementor-aspect-ratio-169 elementor-widget elementor-widget-video\" data-id=\"44842af\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/9kMMicQfgJM&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;,&quot;aspect_ratio&quot;:&quot;169&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2ed8553 elementor-widget elementor-widget-text-editor\" data-id=\"2ed8553\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>FPLC<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-04146fb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"04146fb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-f98f82c\" data-id=\"f98f82c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0fa249e elementor-aspect-ratio-169 elementor-widget elementor-widget-video\" data-id=\"0fa249e\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/17G91DI1j2w&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;,&quot;aspect_ratio&quot;:&quot;169&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0c62957 elementor-widget elementor-widget-text-editor\" data-id=\"0c62957\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Column Chromatography<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-4f1c701\" data-id=\"4f1c701\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-268f9ba\" data-id=\"268f9ba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4cc8f2c elementor-aspect-ratio-169 elementor-widget elementor-widget-video\" data-id=\"4cc8f2c\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/ohQKaTeAhzQ&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;,&quot;aspect_ratio&quot;:&quot;169&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9a32857 elementor-widget elementor-widget-text-editor\" data-id=\"9a32857\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Batch Spin Chromatography<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e2a9360 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e2a9360\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-eb10729\" data-id=\"eb10729\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-511e0e8 elementor-widget elementor-widget-text-editor\" data-id=\"511e0e8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #0000ff;\"><strong>FAQ<\/strong><\/span><\/p>\n<p><strong>&#8211; \u00bfPor qu\u00e9 deber\u00eda usar perlas de Co-NTA en lugar de Ni-NTA?<\/strong><\/p>\n<p>R: Como ilustra la figura 1 en la secci\u00f3n de &#8220;resultados de laboratorio&#8221;, las purificaciones de Co-NTA que utilizan la etiqueta His son m\u00e1s puras que las de Ni-NTA en comparaci\u00f3n. Esto tiene un costo de parte del rendimiento de prote\u00edna. Si sus necesidades se ajustan a ese patr\u00f3n, use perlas de Co-NTA en lugar de Ni-NTA.<\/p>\n<p><strong>&#8211; \u00bfCu\u00e1les son las razones de la uni\u00f3n no espec\u00edfica?<\/strong><\/p>\n<p>R: Aunque Co-NTA es la forma m\u00e1s pura de purificaci\u00f3n de etiquetas His, algunas prote\u00ednas no deseadas a\u00fan pueden unirse a las perlas. Pero el lavado con NaOH despu\u00e9s de la eluci\u00f3n de la prote\u00edna de inter\u00e9s elimina las prote\u00ednas unidas inespec\u00edficas de la resina.<\/p>\n<p><strong>&#8211; Quiero usar una alta concentraci\u00f3n de EDTA y DTT. \u00bfEs posible utilizar Co-NTA de Cube Biotech?<\/strong><\/p>\n<p>R: No, no se recomienda porque los iones de cobalto se reducen con DTT o se disuelven con EDTA. Si desea utilizar altas concentraciones de EDTA y DTT, debe utilizar nuestra\u00a0<a title=\"\" href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/his-tag-affinity-resins-magbeads\/indigo-ni\/purecube-100-indigo-ni-agarose\/75103\" target=\"_blank\" rel=\"noopener\">resina Indigo.<\/a><\/p>\n<p><strong>-\u00bfC\u00f3mo es la capacidad the flujos elevados?<\/strong><\/p>\n<p>R: Si se desean velocidades de flujo m\u00e1s altas, recomendamos usar perlas con di\u00e1metros m\u00e1s grandes.<\/p>\n<p>Ofrecemos perlas de Co-NTA con di\u00e1metros medios de 40 \u00b5m, 100 \u00b5m y 400 \u00b5m (XL).<\/p>\n<p>Con cada aumento de tama\u00f1o, las velocidades de flujo tambi\u00e9n aumentan debido al espacio proporcionalmente creciente entre las perlas. Sin embargo, la superficie de las perlas no aumenta a la misma velocidad que el di\u00e1metro (ley del cuadrado-cubo). Eso da como resultado cantidades decrecientes de prote\u00edna purificada por ml de perlas mientras aumenta el tama\u00f1o de las perlas.<\/p>\n<p>Para 40 \u00b5m de perlas de 100 \u00b5m, ambos tenemos cantidades de purificaci\u00f3n promedio de ~30 \u00b5g de prote\u00edna\/mL de perlas. Con perlas de 400 \u00b5m (XL), esto disminuye a una cantidad no especificada.<\/p>\n<p>Recomendamos leer el apartado correspondiente de la gu\u00eda &#8220;Introducci\u00f3n a las matrices de agarosa&#8221; sobre este tema para obtener informaci\u00f3n m\u00e1s detallada.<\/p>\n<p>&#8211;\u00a0<strong>Despu\u00e9s de usar DTT mi resina cambi\u00f3 de color. \u00bfC\u00f3mo regenerarlo?<\/strong><\/p>\n<p>R: Seguramente la TDT ha da\u00f1ado las perlas. Las perlas de Co-NTA solo tienen una tolerancia DTT limitada de aproximadamente 1 mM. Sin embargo, puede regenerarlos para recuperar su funcionalidad. Lea nuestro protocolo detallado para obtener m\u00e1s informaci\u00f3n al respecto.<\/p>\n<p>Sin embargo, recomendamos usar productos Ni-INDIGO en su lugar. Trabajan con los mismos buffers y protocolos que los productos Ni-NTA pero tienen una tolerancia DTT de 20 mM.<\/p>\n<p>\u00a0<\/p>\n<p><strong style=\"color: #0000ff;\"><mark class=\"has-inline-color has-vivid-red-color\">Descargo de responsabilidad:<\/mark><\/strong><\/p>\n<div class=\"wp-block-group\">\n<p class=\"has-background\"><span style=\"color: #0000ff;\"><strong>Nuestros productos est\u00e1n destinados para investigaci\u00f3n en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/strong><\/span><\/p>\n<p>\u00a0<\/p>\n<\/div>\n<p>\u00a0<\/p>\n<p>\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Resina de agarosa PureCube Co-NTA XL, desarrollada para la purificaci\u00f3n por afinidad de prote\u00ednas portadoras de etiqueta de polihistidina.<\/p>\n","protected":false},"featured_media":10258,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/10255"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":32,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/10255\/revisions"}],"predecessor-version":[{"id":10431,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/10255\/revisions\/10431"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/10258"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=10255"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=10255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}