El colorante iFluor 610 en una excelente alternativa al colorante de etiquetado Alexa Fluor® 610
Descripción
Los tintes iFluor® de AAT Bioquest están optimizados para marcar proteínas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento mínimo de las proteínas. Pueden excitarse bien con las principales líneas láser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm).
Los colorantes iFluor® 610 tienen un máximo de excitación y emisión de fluorescencia de ~610 nm y ~628 nm respectivamente. Estas características espectrales los convierten en un excelente reemplazo para el colorante de etiquetado Alexa Fluor® 610 (Alexa Fluor® es la marca registrada de Invitrogen).
iFluor® 610 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de proteínas.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-1038 | iFluor® 610 succinimidyl ester | 1mg |
| AAT-71038 | iFluor® 610 succinimidyl ester | 100 ug |
| AAT-71516 | iFluor® 610 succinimidyl ester | 5mg |
| AAT-71566 | iFluor® 610 succinimidyl ester | 10mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 1253.52 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Factor de corrección (260 nm) | 0.32 |
| Factor de corrección (280 nm) | 0.49 |
| Coeficiente de extinción (cm -1 M -1) | 1100001 |
| Excitación (nm) | 610 |
| Emisión (nm) | 628 |
| Rendimiento cuántico | 0.851 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de succinimidil éster iFluor® 610 a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 79.775 µL | 398.877 µL | 797.754 µL | 3.989 mL | 7.978 mL |
| 5 mM | 15.955 µL | 79.775 µL | 159.551 µL | 797.754 µL | 1.596 mL |
| 10 mM | 7.978 µL | 39.888 µL | 79.775 µL | 398.877 µL | 797.754 µL |
Imagenes
Figura 1. Las células HeLa se tiñeron con antitubulina de ratón seguido de iFluorTM 610 de cabra anti-ratón IgG (H+L), y los núcleos se tiñeron con Nuclear GreenTM DCS1 (Cat# 17550).
Figura 2. Las células HeLa se tiñeron con (Tubulin+) o sin (Tubulin-) antitubulina de ratón y luego se visualizaron con iFluor® 610 cabra anti-ratón IgG (derecha) o Alexa Fluor® 610 cabra anti-ratón IgG (izquierda).
Figura 3. Arriba) El patrón espectral se generó usando un citómetro espectral de 4 láseres. Se utilizaron láseres desplazados espacialmente (355 nm, 405 nm, 488 nm y 640 nm) para crear cuatro perfiles de emisión distintos y luego, cuando se combinaron, produjeron la firma espectral general. Abajo) Análisis de citometría de flujo de células de sangre completa teñidas con conjugado de PE/iFluor® 610 anti-CD4 humano SK3. La señal de fluorescencia se controló utilizando un citómetro de flujo Aurora en el canal B6-A específico de PE/iFluor® 610.
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Bibliografiía
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
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Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
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Bibliografía
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153–0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011–E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425–E436
Referencias
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Application Notes
iFluor® Dye Selection Guide
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
FAQ
What are common laser lines used in flow cytometry?
What are the spectral properties of iFluor dyes?
Are any of the cyanine dyes infrared?
Are coumarin dyes pH sensitive?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
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