Equivalent a Cy7® bisNHS ester
Descripción
Se ha utilizado una variedad de tintes de cianina 7 (Cy7®) para marcar moléculas biológicas para imágenes de fluorescencia y otros análisis bioquímicos basados en fluorescencia. Se utilizan ampliamente para marcar péptidos, proteínas y oligos, etc. Los conjugados de colorante Cy7® son uno de los fluoróforos rojos del infrarrojo cercano más comunes utilizados en aplicaciones de imágenes in vivo. El éster Cy7® NHS reacciona fácilmente con los grupos amino.
AAT Bioquest ofrece ésteres de colorante Cy NHS en forma de sales de trietilamonio que son más solubles en DMSO y DMF que las sales de potasio correspondientes que ofrecen otros proveedores. Las sales de trietilamonio del tinte Cy tienen la misma reactividad y dan los conjugados idénticos a las sales de potasio del tinte Cy. Cy7® es la marca registrada de GE Healthcare.
| Catalogo | Producto | Presentacion |
|---|---|---|
| AAT-170 | Cyanine 7 bissuccinimidyl ester | 1mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelacion (< -15 °C). Minimizar la exposición a la luz.
Propiedades fisicas
| Peso Molecular | 1064.27 |
| Disolvente | DMSO |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades Espectrales
| Factor de Corrección (260 nm) | 0.05 |
| Factor de Corrección (280 nm) | 0.036 |
| Factor de Corrección (482 nm) | 0.0005 |
| Factor de Corrección (565 nm) | 0.0193 |
| Factor de Corrección (650 nm) | 0.165 |
| Coeficiente de extinción (cm -1 M -1) | 250000 |
| Excitación (nm) | 756 |
| Emisión (nm) | 779 |
| Rendimiento Cuántico | 0.3 |
Calculadora
Preparación de la solución de stock común
Volumen de DMSO necesario para reconstituir la masa específica de Cyanine 7 bissuccinimidil éster [equivalente a Cy7® bisNHS éster] a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 93.961 µL | 469.806 µL | 939.611 µL | 4.698 mL | 9.396 mL |
| 5 mM | 18.792 µL | 93.961 µL | 187.922 µL | 939.611 µL | 1.879 mL |
| 10 mM | 9.396 µL | 46.981 µL | 93.961 µL | 469.806 µL | 939.611 µL |
Estructura química del éster de bissuccinimidilo de cianina 7 [equivalente al éster de bisNHS de Cy7®]
Productos Similares
| Nombre | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
| Cyanine 3 bissuccinimidyl ester [equivalent to Cy3® bisNHS ester] | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | – | – |
| Cyanine 5 bissuccinimidyl ester [equivalent to Cy5® bisNHS ester] | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
| Cyanine 7 monosuccinimidyl ester [equivalent to Cy7® NHS ester] | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 | 0.0005 | 0.0193 |
Bibliografía
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Journal: Journal of Biomaterials Applications (2017): 923–932
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Journal: Colloids and Surfaces B: Biointerfaces (2017)
Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
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Authors: Nagamine, Takeaki and Hayakawa, Kou and Nakazato, Kyoumi and Iha, Masahiko
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Referencias
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Journal: Appl Phys Lett (2011): 143703
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Journal: Biochemistry (2011): 2691
In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
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Journal: Electrophoresis (2008): 4516
Surface plasmon resonance-enhanced fluorescence implementation of a single-step competition assay: demonstration of fatty acid measurement using an anti-fatty acid monoclonal antibody and a Cy5-labeled fatty acid
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Cy7-Bis-dipicolylamine-zinc
Authors: Leung K., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Cy7-Tetrameric arginine-glycine-aspartic acid peptide
Authors: Cheng KT., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)
Application Notes (en Ingles)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)