Calbryte™ 590 es una nueva generación de indicadores fluorescentes rojos para la medición del calcio intracelular.
Descripción
El ensayo de flujo de calcio intracelular es un método ampliamente utilizado en el monitoreo de las vías de transducción de señales y el cribado de alto rendimiento de los receptores acoplados a proteínas G (GPCR) y los objetivos de los canales de calcio. Seguidos por la introducción de Rhod-2 en 1989, Rhod-4 y Cal-590 se desarrollaron más tarde con una relación señal / fondo mejorada, y se convirtieron en los indicadores fluorescentes rojos de Ca2 + ampliamente utilizados para microscopía confocal, citometría de flujo y aplicaciones de detección de alto rendimiento.
Calbryte™ 590 es una nueva generación de indicadores fluorescentes rojos para la medición del calcio intracelular. Su relación señal/fondo muy mejorada y sus propiedades de retención intracelular hacen de Calbryte™ 590 el indicador fluorescente rojo más robusto para evaluar GPCR y objetivos de canales de calcio, así como para detectar sus agonistas y antagonistas en células vivas.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-20706 | Calbryte™ 590, potassium salt | 5 x 50 ug |
| AAT-20707 | Calbryte™ 590, potassium salt | 1 mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a (< -15 °C), Minimizar la exposición a la luz.
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Excitación (nm) | 581 |
| Emisión (nm) | 593 |
Calculadora
Preparación de la solución de stock común
Volumen de agua necesario para reconstituir la masa específica de Calbryte™ 590, sal de potasio a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para obtener los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 93.025 µL | 465.125 µL | 930.25 µL | 4.651 mL | 9.302 mL |
| 5 mM | 18.605 µL | 93.025 µL | 186.05 µL | 930.25 µL | 1.86 mL |
| 10 mM | 9.302 µL | 46.512 µL | 93.025 µL | 465.125 µL | 930.25 µL |
Imagen
Figura 1. Espectros de emisión de fluorescencia de Calbryte™ 590 en solución que contiene 0 a 39 μM libre Ca2+.
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Referencias
Ver todas las 53 referencias: Citation Explorer
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Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
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MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
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