Calbryte™-520L, potassium salt

Indicador de calcio Calbryte™-520L sal de potasio

Descripción

El ensayo de flujo de calcio intracelular es un método ampliamente utilizado para monitorear las vías de transducción de señales y el cribado de alto rendimiento de los receptores acoplados a proteínas G (GPCR) y los objetivos de los canales de calcio. Tras la introducción de Fluo-3 en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron más tarde con relaciones señal-fondo mejoradas y pronto se convirtieron en los indicadores de Ca2+ de elección para microscopía confocal, citometría de flujo y aplicaciones de screening de alto rendimiento.

Calbryte 520L tiene una baja afinidad con los iones de calcio con un Kd ∼91 μM. Calbryte 520L produce una señal de fluorescencia brillante en presencia de calcio a una alta concentración. Tiene una longitud de onda de excitación y emisión idéntica a la de Fluo-4; por lo tanto, los mismos ajustes de ensayo Fluo-4 se pueden aplicar fácilmente a los ensayos de calcio basados en Calbryte 520L.

Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a  (< -15 °C), Minimizar la exposición a la luz.

CatalogoProductoPresentación
AAT-20642Calbryte™-520L, potassium salt10 x 50 ug

Espectro

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Propiedades espectrales

Excitación (nm)493
Emisión (nm)515
Rendimiento cuántico0.751
1 Calcium bound (pH 7.2)

Calculadora

Volumen de agua necesario para reconstituir la masa específica de Calbryte-520L™, sal de potasio a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para obtener los buffers experimentales/fisiológicos apropiados.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM125.805 µL629.026 µL1.258 mL6.29 mL12.581 mL
5 mM25.161 µL125.805 µL251.61 µL1.258 mL2.516 mL
10 mM12.581 µL62.903 µL125.805 µL629.026 µL1.258 mL

Imagen

Figura 1. Ca2+ Dosis-respuesta dependiente de Calbryte 520

Productos Relacionados

NameExcitation (nm)Emission (nm)Quantum yield
Calbryte™ 520, potassium salt4935150.751
Calbryte™ 590, potassium salt581593
Calbryte™ 630, potassium salt607624
Calbryte™-520XL, potassium salt4935150.751
1 Calcium bound (pH 7.2)

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Cal-520®-Biotin Conjugate
Cal-520®-Biocytin Conjugate
Cal-520® NHS Ester
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Bibliografía

Calreticulin regulates TGF-&beta;1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry &amp; Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861–6868

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581–594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537–542

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593–600

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441–447

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771–778

Referencias

Ver todas las 53 referencias: Citation Explorer

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Journal: J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
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Journal: Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
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Journal: Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
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Journal: Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
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Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
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Journal: Mol Reprod Dev (2000): 335

MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
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Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
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Journal: Mol Reprod Dev (1999): 418

Aplication Notes (en Ingles)

A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets