Calbryte™ 520 es una nueva generación de indicadores fluorescentes para mediciones de calcio, su relación señal/ruido muy mejorada y mejores propiedades de retención intracelular hacen de Calbryte™ 520 el indicador de calcio más sólido.
Descripción
La medición del calcio es fundamental para numerosas investigaciones biológicas. Las sondas fluorescentes que muestran respuestas espectrales al unirse al calcio han permitido a los cientificos investigar los cambios en las concentraciones de calcio libre celular mediante microscopía de fluorescencia, citometría de flujo, espectroscopía de fluorescencia y lectores de microplacas de fluorescencia.
Seguido por la introducción de Fluo-3 en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron más tarde con una mejor relación señal/fondo. Sin embargo, todavía hay algunos problemas graves con Fluo-4. Por ejemplo, en cuanto a Fluo-3, en la mayoría de los ensayos de calcio intracelular con Fluo-4, se requiere probenecid para evitar que el Fluo-4 cargado en las células se escape de las células. El uso de probenecid con ensayos de calcio basados en Fluo-4 compromete los resultados del ensayo ya que está bien documentado que el probenecid tiene una variedad de efectos celulares complicados.
Calbryte™ 520 es una nueva generación de indicadores fluorescentes para mediciones de calcio, su relación señal/ruido muy mejorada y mejores propiedades de retención intracelular hacen de Calbryte™ 520 el indicador de calcio más sólido. Tiene la misma longitud de onda de excitación y emisión que Fluo-4, por lo que la misma configuración de ensayo de Fluo-4 se puede aplicar fácilmente a los ensayos de calcio basados en Calbryte™ 520.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-20656 | Calbryte™ 520, potassium salt | 2 x 50 ug |
| AAT-20658 | Calbryte™ 520, potassium salt | 10 x 50 ug |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelar a (< -15 °C), Minimizar la exposición a la luz.
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Excitación (nm) | 493 |
| Emisión (nm) | 515 |
| Rendimiento cuántico | 0.751 |
Calculadora
Preparación de la solución de stock común
Volumen de agua necesario para reconstituir la masa específica de Calbryte™ 520, sal de potasio a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 110.009 µL | 550.043 µL | 1.1 mL | 5.5 mL | 11.001 mL |
| 5 mM | 22.002 µL | 110.009 µL | 220.017 µL | 1.1 mL | 2.2 mL |
| 10 mM | 11.001 µL | 55.004 µL | 110.009 µL | 550.043 µL | 1.1 mL |
Imagen
Figura 1. Espectros de emisión de fluorescencia de Calbryte™ 520 en una solución que contiene de 0 a 39 µM de Ca2+ libre.
Productos Relacionados
| Name | Excitation (nm) | Emission (nm) | Quantum yield |
| Calbryte™ 590, potassium salt | 581 | 593 | – |
| Calbryte™ 630, potassium salt | 607 | 624 | – |
| Calbryte™-520L, potassium salt | 493 | 515 | 0.751 |
| Calbryte™-520XL, potassium salt | 493 | 515 | 0.751 |
| Cal-520®, potassium salt | 493 | 515 | 0.751 |
| Mag-520™ potassium salt | 507 | 531 | – |
Bibliografía
Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: The International Journal of Biochemistry & Cell Biology (2017)
Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: Neural Regeneration Research (2017): 945
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: Scientific Reports (2017): 41781
Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: Int J Clin Exp Med (2017): 6861–6868
Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: Journal of cellular and molecular medicine (2015): 581–594
The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: Biochemical and biophysical research communications (2015): 537–542
Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: Journal of Neural Transmission (2014): 593–600
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: Neural regeneration research (2014): 2002
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: International journal of ophthalmology (2014): 441–447
Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: British Journal of Dermatology (2013): 771–778
Referencias
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Aplication Notes (en Ingles)
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets