Amplite® Blue permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD).
Descripción
Hay una variedad de sustratos disponibles para detectar la actividad de la peroxidasa en los ensayos ELISA.
Los sustratos colorimétricos de HRP (p. ej., TMB, OPD y ABTS) se han utilizado ampliamente durante años. Cada uno de estos sustratos varía mucho con respecto a sus características de rendimiento, como la sensibilidad de detección, el rango de trabajo y las relaciones señal/ruido alcanzables.
Nuestro sustrato de peroxidasa Amplite® Blue es un sustrato fluorogénico soluble para la detección de la actividad de la peroxidasa. Amplite® Blue permite realizar ensayos de detección rápida de HRP con mayor sensibilidad que los sustratos colorimétricos de HRP (como TMB y OPD). El producto fluorescente de Amplite® Blue no fotoblanquea. Amplite® Blue exhibe una línea de base plana en los ensayos, lo que facilita la sensibilidad de detección de bajo nivel y permite altas relaciones señal-ruido.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-11005 | Amplite® Blue AAT Bioquest | 25 mg |
Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelación a <-15 °C. Minimizar la exposición a la luz.
Plataforma
Lector de Microplacas de Flourescencia
| Excitación | 324 nm |
| Emisión | 409 nm |
| Cuttoff | 350 nm |
| Placa recomendada | Placa negra |
Notas importantes
Es importante almacenar a <-15 °C y debe almacenarse en un lugar fresco y oscuro.
Se puede utilizar en un plazo de 12 meses a partir de la fecha de recepción.
Imagen
Figura 1. La respuesta a la dosis de HRP se midió con el kit de ensayo de peroxidasa fluorimétrica Amplite® en una placa negra utilizando un lector de microplacas de fluorescencia Gemini (Molecular Devices).
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Referencias
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Application notes
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