Colorante catiónico que se acumula selectivamente en las mitocondrias, probablemente a través del gradiente de potencial de la membrana mitocondrial.
Descripción
MitoLite™ Orange 405 es un colorante catiónico que se acumula selectivamente en las mitocondrias, probablemente a través del gradiente de potencial de la membrana mitocondrial. El indicador mitocondrial es un compuesto hidrofóbico que penetra fácilmente en las células vivas intactas y queda atrapado en las mitocondrias después de que ingresa a las células. Este indicador mitocondrial fluorescente se retiene en las mitocondrias durante mucho tiempo ya que el indicador lleva un grupo de retención celular. Esta característica clave aumenta significativamente su eficacia de tinción. El protocolo de etiquetado es sólido y requiere un tiempo mínimo de manipulación.
Puede adaptarse fácilmente a una amplia variedad de plataformas de fluorescencia, como ensayos en microplaca, inmunocitoquímica y citometría de flujo. Es adecuado para células proliferantes y no proliferativas, y se puede utilizar tanto para células en suspensión como adherentes. MitoLite™ Orange 405 está excitado por el láser violeta a 405 nm, por lo que es particularmente útil para los citómetros de flujo con una fuente de láser violeta.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-22679 | MitoLite™ Orange EX405 | 500 pruebas |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Exitación | 425 |
| Emisión | 522 |
Imagen
Figura 1.
Imagen de fluorescencia de células HeLa teñidas con MitoLite™ Orange EX405 en una placa de 96 pocillos de pared negra/fondo transparente.
Productos Relacionados
| MitoLite™ Orange FX570 |
| MitoLite™ Blue FX490 |
| MitoLite™ Green EX488 |
| MitoLite™ Red FX600 |
| MitoLite™ Deep Red FX660 |
| MitoLite™ NIR FX690 |
| MitoLite™ Green FM |
| MitoLite™ Red CMXRos |
Bibliografía
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Dual-Responsive Fluorescent Probe for Imaging NAD (P) H and Mitochondrial Viscosity and Its Application in Cancer Cell Ferroptosis
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Journal: Sensors and Actuators B: Chemical (2021): 130862
Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
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Journal: Colloids and Surfaces B: Biointerfaces (2017): 131–140
Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin
Authors: Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: Apoptosis (2016): 489–501
Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers
Authors: Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: Journal of medicinal chemistry (2015): 7949–7958
Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes
Authors: Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: Journal of Pineal Research (2015): 267–275
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Application notes (en Ingles)
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
Calcein