6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]

6-ROX se usa predominantemente para marcar nucleótidos y secuenciar ácidos nucleicos. *CAS#: 216699-36-4*

Descripción

Los isómeros únicos de ROX se prefieren cada vez más para marcar péptidos y nucleótidos porque brindan una mejor resolución en las purificaciones de HPLC que a menudo se requieren en las bioconjugaciones.

6-ROX, SE es el otro isómero único purificado de 5(6)-ROX, SE. 6-ROX se usa predominantemente para marcar nucleótidos y secuenciar ácidos nucleicos. (Ver cat. AAT-389 para 5-ROX SE)

En comparación con otras rodaminas, ROX es muy inestable. Se deben tomar precauciones para evitar el deterioro durante el almacenamiento.

Catalogo	Producto	Presentación
AAT-392	6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]	5 mg
AAT-399	6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]	100 mg

SDS

Protocol

Importante, Solo para uso en investigación (RUO). Almacenamiento: Congelar (< -15 °C), Minimizar la exposición a la luz

Propiedades fisicas

Peso Molecular	631.67
Disolvente	DMSO

Calculadora

Preparación de la solución de stock común.
Volumen de DMSO necesario para reconstituir la masa específica de 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* a la concentración dada. Tenga en cuenta que el volumen es solo para preparar la solución madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales/fisiológicos apropiados.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	158.311 µL	791.553 µL	1.583 mL	7.916 mL	15.831 mL
5 mM	31.662 µL	158.311 µL	316.621 µL	1.583 mL	3.166 mL
10 mM	15.831 µL	79.155 µL	158.311 µL	791.553 µL	1.583 mL

Espectro

Abrir en Advanced Spectrum Viewer

Propiedades Espectrales

Factor de correción (280 nm)	0.168
Coeficiente de extinción (cm ^-1 M ^-1)	82000
Excitación (nm)	578
Emisión (nm)	604
Rendimiento cuántico	0.94¹

1 Buffer acuoso (pH 7.2)

Imagen

Figura 1. Estructura quimica de 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS 216699-36-4*

Productos Relacionados

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] Single isomer	522	546	94000	0.214
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] CAS#: 150810-69-8	552	578	90000	0.178
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] CAS 198978-94-8	578	604	82000	0.168
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] CAS 209734-74-7	578	604	82000	0.168
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS 92557-81-8	493	517	83000	0.178
6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] Single Isomer	533	559	73000	0.15
6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] CAS#: 113394-23-3	520	545	75000¹	–
6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester]	521	542	73000	0.13
6-ROX glycine 25 uM fluorescence reference solution for PCR reactions	578	604	82000	0.168
6-ROX azide	578	604	82000	0.168
6-ROX alkyne	578	604	82000	0.168
6-ROXtra™ SE Superior 6-ROX Replacement	578	595	–	–
6-VIC, SE [6-VIC NHS ester]	526	543	–	–
6-NED, SE [6-NED NHS ester]	545	567	74000²

¹Buffer pH9, ² PBS

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5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*

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Phalloidin-Tetramethylrhodamine Conjugate

5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]

6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]

5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]

6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]

Bis-L-Aspartic acid Beta-rhodamine 110

Bis-Arg-rhodamine 110

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5-Carboxyrhodamine 6G maleimide

Rhodamine aldehyde [5-TAMRA aldehyde]

Referencias

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926

Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
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Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
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HLA-DRB fluorotyping by dark quenching and automated analysis
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Journal: Tissue Antigens (2001): 250

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction–amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32

Application Notes

Sunnyvale Red™ SE, a Superior Replacement for 6-ROX SE