Este producto TQ3 se utiliza principalmente para el marcaje de oligonucleótidos durante la síntesis.
Descripción
TQ3 está diseñado para ser un extintor superior a TAMRA, TF3 y Cy3. TQ3 tiene (a). absorción mucho más fuerte; (b). mayor eficiencia de extinción; y c). formas reactivas versátiles con la solubilidad deseada para marcar oligonucleótidos y péptidos. El producto TQ3 se utiliza principalmente para el marcaje de oligonucleótidos durante la síntesis.
| Catalogo | Producto | Presentación |
|---|---|---|
| AAT-2223 | Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å* | 100 mg |
Importante: Solo para uso en investigación (RUO). Almacenamiento: Congelación (< -15 °C). Minimizar la exposición a la luz.
Propiedades físicas
| Peso Molecular | N/D |
| Disolvente | MeCN |
Espectro
Abrir en Advanced Spectrum Viewer
Propiedades espectrales
| Absorbancia | 573 |
| Factor de corrección (260 nm) | 0.085 |
| Factor de corrección (280 nm) | 0.091 |
| Coeficiente de extinción (cm -1 M -1) | 22000 |
Imagen
Figura 1.
La síntesis de oligonucleótidos se lleva a cabo mediante la adición gradual de residuos de nucleótidos al 5′-terminus de la cadena en crecimiento hasta que se ensambla la secuencia deseada. Cada adición se denomina ciclo sintético y consta de cuatro reacciones químicas: desbloqueo (destritilación), acoplamiento, tapado y oxidación.
Productos Relacionados
| Nombre | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å* | 23000 | 0.146 | 0.183 |
| Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å* | 23000 | 0.146 | 0.183 |
| Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å* | – | – | – |
| Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å* | – | – | – |
| Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å* | 20000 | 0.147 | 0.194 |
| Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å* | 20000 | 0.147 | 0.194 |
| Tide Quencher™ 2 acid [TQ2 acid] | 21000 | 0.100 | 0.12 |
| Tide Quencher™ 2 amine [TQ2 amine] | 21000 | 0.100 | 0.12 |
| Tide Quencher™ 3 acid [TQ3 acid] | 22000 | 0.085 | 0.091 |
Bibliografía
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Application Notes (en Ingles)
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V